dbPTM

WNK3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	WNK3_HUMAN
UniProt AC	Q9BYP7
Protein Name	Serine/threonine-protein kinase WNK3 {ECO:0000305}
Gene Name	WNK3 {ECO:0000312\|HGNC:HGNC:14543}
Organism	Homo sapiens (Human).
Sequence Length	1800
Subcellular Localization	Cytoplasm .
Protein Description	Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. [PubMed: 16275913]
Protein Sequence	MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGLQKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAALLKPDLVRSLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSSQTGSSEQVQINSTSTQTSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQHSMSAVPGRHPLPSPKNTSNKEISRDTLLTIENNPCHRALFTSKSEHKDVVDGKISECASVETKQPAILYQVEDNRQIMAPVTNSSSYSTTSVRAVPAECEGLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGNSLTTLAFDQKPQTLSVQQPAMDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQPTTVLESDGERPPKLEFADNRIKTLDEKLRNLLYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVAASQPANISVFKRDLNVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAIHLQTGGGYFGLSFTCPSLKNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEEMRSAIAPDPIPLTRESTADTRALNRCKAMSGSFQRGRFQVITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKSQLVEIEPATQNPKTSFSYEKLQALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPEKEFEETSATGSSMQSGSELLLKEREILTAGKQPSSDSEFSASLAGSGKSVAKTGPESNQCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQRLREKHIQEVVNLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSKLRSRPQSLTHVDNGIVATGKSCLINELENPLCVESNAASCQQSPASKKGMFTDDLHKLVDDWTKEAVGNSLIKPSLNQLKQSQHKLETENWNKVSENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAGYPVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGPK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MATDSGDPAS -----CCCCCCCCCC	42.42	29507054
5	Phosphorylation	---MATDSGDPASTE ---CCCCCCCCCCCC	40.52	29507054
10	Phosphorylation	TDSGDPASTEDSEKP CCCCCCCCCCCCCCC	37.40	29507054
11	Phosphorylation	DSGDPASTEDSEKPD CCCCCCCCCCCCCCC	46.01	29507054
14	Phosphorylation	DPASTEDSEKPDGIS CCCCCCCCCCCCCCC	41.09	29507054
44	Phosphorylation	ARLKEKNSTFSASGE EHHHHCCCCCCCCCC	41.73	28509920
45	Phosphorylation	RLKEKNSTFSASGET HHHHCCCCCCCCCCC	30.88	28509920
47	Phosphorylation	KEKNSTFSASGETVE HHCCCCCCCCCCCHH	22.93	28857561
49	Phosphorylation	KNSTFSASGETVERK CCCCCCCCCCCHHHH	35.64	24114839
52	Phosphorylation	TFSASGETVERKRFF CCCCCCCCHHHHHHH	31.06	28509920
62	Phosphorylation	RKRFFRKSVEMTEDD HHHHHHHCCCCCCCC	21.03	29978859
66	Phosphorylation	FRKSVEMTEDDKVAE HHHCCCCCCCCHHHC	23.82	24719451
77	Acetylation	KVAESSPKDERIKAA HHHCCCCHHHHHHHH	74.33	19413330
140	Phosphorylation	AEMKAVATSPSGRFL HHHHHHEECCCCCEE	34.27	22210691
141	Phosphorylation	EMKAVATSPSGRFLK HHHHHEECCCCCEEE	13.82	-
143	Phosphorylation	KAVATSPSGRFLKFD HHHEECCCCCEEEEE	42.22	22210691
215	Phosphorylation	RFYDSWESILKGKKC HHHHHHHHHHCCCCE	27.28	24719451
227	Phosphorylation	KKCIVLVTELMTSGT CCEEEEEEECCCCCH	21.17	30387612
232	Phosphorylation	LVTELMTSGTLKTYL EEEECCCCCHHHHHH	18.80	24719451
234	Phosphorylation	TELMTSGTLKTYLKR EECCCCCHHHHHHHH	25.50	24719451
238	Phosphorylation	TSGTLKTYLKRFKVM CCCHHHHHHHHHCCC	14.51	30387612
252	Phosphorylation	MKPKVLRSWCRQILK CCHHHHHHHHHHHHH	26.77	22964224
299	Phosphorylation	IGDLGLATLMRTSFA ECHHHHHHHHHHHHH	26.57	22199227
303	Phosphorylation	GLATLMRTSFAKSVI HHHHHHHHHHHHHHC	17.77	29514088
304	Phosphorylation	LATLMRTSFAKSVIG HHHHHHHHHHHHHCC	16.91	24719451
308	Phosphorylation	MRTSFAKSVIGTPEF HHHHHHHHHCCCCHH	18.61	-
343	Phosphorylation	MCMLEMATSEYPYSE HHHHHHHCCCCCHHH	22.30	26552605
344	Phosphorylation	CMLEMATSEYPYSEC HHHHHHCCCCCHHHH	26.12	26552605
346	Phosphorylation	LEMATSEYPYSECQN HHHHCCCCCHHHHHC	13.77	26552605
348	Phosphorylation	MATSEYPYSECQNAA HHCCCCCHHHHHCHH	19.05	26552605
349	Phosphorylation	ATSEYPYSECQNAAQ HCCCCCHHHHHCHHH	27.65	26552605
358	Phosphorylation	CQNAAQIYRKVTSGI HHCHHHHHHHHHCCC	7.72	26552605
362	Phosphorylation	AQIYRKVTSGIKPAS HHHHHHHHCCCCCCC	25.03	23909892
363	Phosphorylation	QIYRKVTSGIKPASF HHHHHHHCCCCCCCC	41.32	23909892
439	Acetylation	VEDPKKLKGKHKDNE HCCHHHHCCCCCCCC	74.84	20167786
443	Acetylation	KKLKGKHKDNEAIEF HHHCCCCCCCCCEEE	66.78	20167786
468	Acetylation	EVAYEMVKSGFFHES HHHHHHHHCCCCCHH	43.48	20167786
475	Phosphorylation	KSGFFHESDSKAVAK HCCCCCHHHCHHHHH	38.32	-
489	Phosphorylation	KSIRDRVTPIKKTRE HHHHHHCCCCHHCCC	21.86	24719451
509	Phosphorylation	CLEERRDSQCKSMGN CCHHHHHHCCCCCCC	35.35	-
525	Phosphorylation	FPQPQNTTLPLAPAQ CCCCCCCCCCCCCCH	33.65	-
682	Phosphorylation	VPQIKPVSQPVGAEQ CCCCCCCCCCCCHHH	36.98	27251275
701	Phosphorylation	LKPDLVRSLNQDVAT HCHHHHHHCCCCCCC	24.96	27251275
709	O-linked_Glycosylation	LNQDVATTKENVSSP CCCCCCCCCCCCCCC	26.99	21740066
714	Phosphorylation	ATTKENVSSPDNPSG CCCCCCCCCCCCCCC	47.97	22199227
715	Phosphorylation	TTKENVSSPDNPSGN CCCCCCCCCCCCCCC	31.80	22199227
734	Phosphorylation	RIKQRRASCPRPEKG HHHHHHHCCCCCCCC	23.89	18187866
865	Phosphorylation	SNESAPQSSPVGRWR CCCCCCCCCCCCCEE	35.23	24275569
893	Phosphorylation	SPPSLQHSMSAVPGR CCCCHHHHCCCCCCC	11.06	24114839
895	Phosphorylation	PSLQHSMSAVPGRHP CCHHHHCCCCCCCCC	29.18	24114839
905	Phosphorylation	PGRHPLPSPKNTSNK CCCCCCCCCCCCCCC	57.07	24719451
921	Phosphorylation	ISRDTLLTIENNPCH CCCCCEEEECCCHHH	29.38	22985185
974	O-linked_Glycosylation	RQIMAPVTNSSSYST CEEEEEECCCCCCCC	28.46	21740066
1070	Phosphorylation	TVNTEASSPKTVIPT EEECCCCCCCEECCC	37.15	-
1085	Phosphorylation	QTPGLEPTTLQPTTV CCCCCCCCCCCCCEE	30.66	30266825
1086	Phosphorylation	TPGLEPTTLQPTTVL CCCCCCCCCCCCEEE	33.83	30266825
1090	Phosphorylation	EPTTLQPTTVLESDG CCCCCCCCEEEECCC	19.31	30266825
1091	Phosphorylation	PTTLQPTTVLESDGE CCCCCCCEEEECCCC	29.76	30266825
1095	Phosphorylation	QPTTVLESDGERPPK CCCEEEECCCCCCCC	46.91	30266825
1122	Phosphorylation	EKLRNLLYQEHSISS HHHHHHHHHHHCHHH	18.28	29978859
1192	Phosphorylation	LLSNVAASQPANISV HHHHHHHCCCCCEEE	27.27	29396449
1198	Phosphorylation	ASQPANISVFKRDLN HCCCCCEEEEECCCC	22.70	29396449
1288	2-Hydroxyisobutyrylation	RQSTSFFKRSKVRQV HHCCCHHHHHCCEEE	54.47	-
1302	Phosphorylation	VETEEMRSAIAPDPI ECCHHHHHHCCCCCC	23.79	22210691
1312	O-linked_Glycosylation	APDPIPLTRESTADT CCCCCCCCCCCCCCH	27.34	21740066
1315	Phosphorylation	PIPLTRESTADTRAL CCCCCCCCCCCHHHH	26.13	-
1316	Phosphorylation	IPLTRESTADTRALN CCCCCCCCCCHHHHH	24.65	22210691
1329	Phosphorylation	LNRCKAMSGSFQRGR HHHHHHHCCCCCCCC	35.82	27732954
1331	Phosphorylation	RCKAMSGSFQRGRFQ HHHHHCCCCCCCCEE	15.76	30377224
1334	Methylation	AMSGSFQRGRFQVIT HHCCCCCCCCEEEEE	35.92	115920085
1341	Phosphorylation	RGRFQVITIPQQQSA CCCEEEEEECHHHCC	27.96	-
1393	Phosphorylation	ATQNPKTSFSYEKLQ CCCCCCCCCCHHHHH	20.51	27732954
1395	Phosphorylation	QNPKTSFSYEKLQAL CCCCCCCCHHHHHHH	31.95	27732954
1396	Phosphorylation	NPKTSFSYEKLQALQ CCCCCCCHHHHHHHH	18.31	27732954
1470	Phosphorylation	LTAGKQPSSDSEFSA HHCCCCCCCCCCHHH	44.26	30266825
1471	Phosphorylation	TAGKQPSSDSEFSAS HCCCCCCCCCCHHHH	52.41	30266825
1473	Phosphorylation	GKQPSSDSEFSASLA CCCCCCCCCHHHHHC	42.35	30266825
1476	Phosphorylation	PSSDSEFSASLAGSG CCCCCCHHHHHCCCC	16.94	27251275
1538	Phosphorylation	EDLKVELQRLREKHI HHHHHHHHHHHHHHH	28.93	32142685
1576	Phosphorylation	IKDSKTQSTEIPLPP HCCCCCCCCCCCCCC	32.99	24275569
1577	Phosphorylation	KDSKTQSTEIPLPPA CCCCCCCCCCCCCCC	28.28	22210691
1585	Phosphorylation	EIPLPPASPRRPRSF CCCCCCCCCCCCCCH	25.39	30266825
1598	Phosphorylation	SFKSKLRSRPQSLTH CHHHHHHCCCCCCCC	59.76	29978859
1602	Phosphorylation	KLRSRPQSLTHVDNG HHHCCCCCCCCCCCC	37.61	29978859
1604	Phosphorylation	RSRPQSLTHVDNGIV HCCCCCCCCCCCCEE	25.71	29978859
1638	Phosphorylation	NAASCQQSPASKKGM CCHHCCCCCCHHCCC	9.14	21613606
1641	Phosphorylation	SCQQSPASKKGMFTD HCCCCCCHHCCCCCH	37.98	-
1647	Phosphorylation	ASKKGMFTDDLHKLV CHHCCCCCHHHHHHH	21.51	22985185
1652	Acetylation	MFTDDLHKLVDDWTK CCCHHHHHHHCHHHH	58.46	12654585
1665	Phosphorylation	TKEAVGNSLIKPSLN HHHHHHCCCCCCCHH	26.08	24719451
1670	Phosphorylation	GNSLIKPSLNQLKQS HCCCCCCCHHHHHHH	34.64	29978859
1677	Phosphorylation	SLNQLKQSQHKLETE CHHHHHHHHHHHHCC	32.44	29978859

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	KLHL2	O95198	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of WNK3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of WNK3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
NED4L_HUMAN	NEDD4L	physical	24920754

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of WNK3_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1471, AND MASSSPECTROMETRY.	19413330 [Abstract]
"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column."; Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.; Anal. Sci. 24:161-166(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-734, AND MASSSPECTROMETRY.	18187866 [Abstract]