dbPTM

SHOT1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SHOT1_HUMAN
UniProt AC	A0MZ66
Protein Name	Shootin-1 {ECO:0000312\|HGNC:HGNC:29319}
Gene Name	SHTN1 {ECO:0000312\|HGNC:HGNC:29319}
Organism	Homo sapiens (Human).
Sequence Length	631
Subcellular Localization	Perikaryon . Cell projection, axon . Cell projection, growth cone . Cytoplasm, cytoskeleton . Cell projection, filopodium . Cell projection, lamellipodium . Localizes in multiple growth cones at neurite tips before the neuronal symmetry-breaking step
Protein Description	Involved in the generation of internal asymmetric signals required for neuronal polarization and neurite outgrowth. Mediates netrin-1-induced F-actin-substrate coupling or 'clutch engagement' within the axon growth cone through activation of CDC42, RAC1 and PAK1-dependent signaling pathway, thereby converting the F-actin retrograde flow into traction forces, concomitantly with filopodium extension and axon outgrowth. Plays a role in cytoskeletal organization by regulating the subcellular localization of phosphoinositide 3-kinase (PI3K) activity at the axonal growth cone. Plays also a role in regenerative neurite outgrowth. In the developing cortex, cooperates with KIF20B to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in the accumulation of phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the growth cone of primary hippocampal neurons..
Protein Sequence	MNSSDEEKQLQLITSLKEQAIGEYEDLRAENQKTKEKCDKIRQERDEAVKKLEEFQKISHMVIEEVNFMQNHLEIEKTCRESAEALATKLNKENKTLKRISMLYMAKLGPDVITEEINIDDEDSTTDTDGAAETCVSVQCQKQIKELRDQIVSVQEEKKILAIELENLKSKLVEVIEEVNKVKQEKTVLNSEVLEQRKVLEKCNRVSMLAVEEYEEMQVNLELEKDLRKKAESFAQEMFIEQNKLKRQSHLLLQSSIPDQQLLKALDENAKLTQQLEEERIQHQQKVKELEEQLENETLHKEIHNLKQQLELLEEDKKELELKYQNSEEKARNLKHSVDELQKRVNQSENSVPPPPPPPPPLPPPPPNPIRSLMSMIRKRSHPSGSGAKKEKATQPETTEEVTDLKRQAVEEMMDRIKKGVHLRPVNQTARPKTKPESSKGCESAVDELKGILGTLNKSTSSRSLKSLDPENSETELERILRRRKVTAEADSSSPTGILATSESKSMPVLGSVSSVTKTALNKKTLEAEFNSPSPPTPEPGEGPRKLEGCTSSKVTFQPPSSIGCRKKYIDGEKQAEPVVVLDPVSTHEPQTKDQVAEKDPTQHKEDEGEIQPENKEDSIENVRETDSSNC

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MNSSDEEK -------CCCHHHHH	11.22	-
3	Phosphorylation	-----MNSSDEEKQL -----CCCHHHHHHH	37.56	29116813
4	Phosphorylation	----MNSSDEEKQLQ ----CCCHHHHHHHH	44.00	29116813
14	Phosphorylation	EKQLQLITSLKEQAI HHHHHHHHHHHHHHH	36.28	25159151
15	Phosphorylation	KQLQLITSLKEQAIG HHHHHHHHHHHHHHH	30.37	25159151
17	Neddylation	LQLITSLKEQAIGEY HHHHHHHHHHHHHHH	47.77	32015554
17	Ubiquitination	LQLITSLKEQAIGEY HHHHHHHHHHHHHHH	47.77	21906983
17 (in isoform 1)	Ubiquitination	-	47.77	21906983
17 (in isoform 2)	Ubiquitination	-	47.77	21906983
17 (in isoform 3)	Ubiquitination	-	47.77	21906983
17 (in isoform 4)	Ubiquitination	-	47.77	21906983
24	Phosphorylation	KEQAIGEYEDLRAEN HHHHHHHHHHHHHHH	14.99	21945579
24 (in isoform 3)	Phosphorylation	-	14.99	27642862
29	Ubiquitination	GEYEDLRAENQKTKE HHHHHHHHHHHHHHH	27.65	32015554
35	Acetylation	RAENQKTKEKCDKIR HHHHHHHHHHHHHHH	63.31	19829747
37	Acetylation	ENQKTKEKCDKIRQE HHHHHHHHHHHHHHH	49.65	19829755
41	Phosphorylation	TKEKCDKIRQERDEA HHHHHHHHHHHHHHH	3.34	32142685
54 (in isoform 7)	Ubiquitination	-	64.91	21906983
73 (in isoform 7)	Ubiquitination	-	10.46	21906983
82	Phosphorylation	IEKTCRESAEALATK HHHHHHHHHHHHHHH	16.44	28985074
89	Ubiquitination	SAEALATKLNKENKT HHHHHHHHHHHHCHH	44.44	32015554
99	Ubiquitination	KENKTLKRISMLYMA HHCHHHHHHHHHHHH	29.35	30230243
101	Phosphorylation	NKTLKRISMLYMAKL CHHHHHHHHHHHHHH	13.53	22617229
104	Phosphorylation	LKRISMLYMAKLGPD HHHHHHHHHHHHCCC	6.08	30108239
109	Ubiquitination	MLYMAKLGPDVITEE HHHHHHHCCCEEEEE	18.69	32015554
111	Ubiquitination	YMAKLGPDVITEEIN HHHHHCCCEEEEEEC	42.52	30230243
112 (in isoform 7)	Ubiquitination	-	7.03	21906983
121	Ubiquitination	TEEINIDDEDSTTDT EEEECCCCCCCCCCC	58.78	30230243
158	2-Hydroxyisobutyrylation	IVSVQEEKKILAIEL HCCHHHHHHHHHEEH	44.34	-
159	Ubiquitination	VSVQEEKKILAIELE CCHHHHHHHHHEEHH	46.35	30230243
169	Ubiquitination	AIELENLKSKLVEVI HEEHHHHHHHHHHHH	58.06	30230243
170	Ubiquitination	IELENLKSKLVEVIE EEHHHHHHHHHHHHH	34.09	30230243
171	Ubiquitination	ELENLKSKLVEVIEE EHHHHHHHHHHHHHH	55.77	30230243
181	Ubiquitination	EVIEEVNKVKQEKTV HHHHHHHHHHHHHHH	56.86	30230243
187	Phosphorylation	NKVKQEKTVLNSEVL HHHHHHHHHCCHHHH	29.49	29978859
189	Phosphorylation	VKQEKTVLNSEVLEQ HHHHHHHCCHHHHHH	7.65	32142685
191	Phosphorylation	QEKTVLNSEVLEQRK HHHHHCCHHHHHHHH	25.56	29978859
204 (in isoform 7)	Ubiquitination	-	59.46	21906983
211	Ubiquitination	NRVSMLAVEEYEEMQ CCCEEEEHHHHHHHH	5.17	29967540
214	Phosphorylation	SMLAVEEYEEMQVNL EEEEHHHHHHHHHHH	12.04	-
214 (in isoform 3)	Phosphorylation	-	12.04	27642862
228	Ubiquitination	LELEKDLRKKAESFA HHHHHHHHHHHHHHH	48.87	30230243
229	Ubiquitination	ELEKDLRKKAESFAQ HHHHHHHHHHHHHHH	64.73	30230243
230	Ubiquitination	LEKDLRKKAESFAQE HHHHHHHHHHHHHHH	51.45	30230243
233	Phosphorylation	DLRKKAESFAQEMFI HHHHHHHHHHHHHHH	30.64	27251275
249	Phosphorylation	QNKLKRQSHLLLQSS HCHHHHHHHHHHHCC	21.82	25159151
249 (in isoform 2)	Phosphorylation	-	21.82	-
255	Phosphorylation	QSHLLLQSSIPDQQL HHHHHHHCCCCHHHH	30.15	23403867
256	Phosphorylation	SHLLLQSSIPDQQLL HHHHHHCCCCHHHHH	25.77	23403867
263	Ubiquitination	SIPDQQLLKALDENA CCCHHHHHHHHHHHH	2.37	30230243
271	Ubiquitination	KALDENAKLTQQLEE HHHHHHHHHHHHHHH	64.74	29967540
275	Ubiquitination	ENAKLTQQLEEERIQ HHHHHHHHHHHHHHH	45.68	29967540
288	Ubiquitination	IQHQQKVKELEEQLE HHHHHHHHHHHHHHH	64.71	30230243
323	Ubiquitination	DKKELELKYQNSEEK HHHHHHHHHCCHHHH	35.10	30230243
324	Phosphorylation	KKELELKYQNSEEKA HHHHHHHHCCHHHHH	26.08	23403867
332	Ubiquitination	QNSEEKARNLKHSVD CCHHHHHHHHHHHHH	61.14	33845483
335	Ubiquitination	EEKARNLKHSVDELQ HHHHHHHHHHHHHHH	37.07	29967540
337	Phosphorylation	KARNLKHSVDELQKR HHHHHHHHHHHHHHH	29.67	18452278
337 (in isoform 2)	Phosphorylation	-	29.67	-
346	Ubiquitination	DELQKRVNQSENSVP HHHHHHHHHCCCCCC	43.63	32015554
346 (in isoform 5)	Ubiquitination	-	43.63	21906983
348	Phosphorylation	LQKRVNQSENSVPPP HHHHHHHCCCCCCCC	33.28	27174698
351	Phosphorylation	RVNQSENSVPPPPPP HHHHCCCCCCCCCCC	31.76	27174698
372	Phosphorylation	PPPNPIRSLMSMIRK CCCCHHHHHHHHHHH	29.19	27174698
375	Phosphorylation	NPIRSLMSMIRKRSH CHHHHHHHHHHHCCC	19.15	27422710
376 (in isoform 6)	Ubiquitination	-	1.82	21906983
381	Phosphorylation	MSMIRKRSHPSGSGA HHHHHHCCCCCCCCC	42.67	27282143
384	Phosphorylation	IRKRSHPSGSGAKKE HHHCCCCCCCCCCCC	39.92	26091039
386	Phosphorylation	KRSHPSGSGAKKEKA HCCCCCCCCCCCCCC	39.66	23312004
390	Ubiquitination	PSGSGAKKEKATQPE CCCCCCCCCCCCCCC	65.01	29967540
392	Ubiquitination	GSGAKKEKATQPETT CCCCCCCCCCCCCCH	66.66	33845483
394	Phosphorylation	GAKKEKATQPETTEE CCCCCCCCCCCCHHH	56.58	21815630
398	Phosphorylation	EKATQPETTEEVTDL CCCCCCCCHHHHHHH	46.31	20068231
398	Ubiquitination	EKATQPETTEEVTDL CCCCCCCCHHHHHHH	46.31	27667366
398 (in isoform 2)	Phosphorylation	-	46.31	-
399	Phosphorylation	KATQPETTEEVTDLK CCCCCCCHHHHHHHH	28.61	20068231
399 (in isoform 2)	Phosphorylation	-	28.61	-
403	Phosphorylation	PETTEEVTDLKRQAV CCCHHHHHHHHHHHH	38.60	27135362
404	Phosphorylation	ETTEEVTDLKRQAVE CCHHHHHHHHHHHHH	56.20	33259812
406	2-Hydroxyisobutyrylation	TEEVTDLKRQAVEEM HHHHHHHHHHHHHHH	45.32	-
406	Acetylation	TEEVTDLKRQAVEEM HHHHHHHHHHHHHHH	45.32	24430961
406	Methylation	TEEVTDLKRQAVEEM HHHHHHHHHHHHHHH	45.32	24430961
406	Ubiquitination	TEEVTDLKRQAVEEM HHHHHHHHHHHHHHH	45.32	21906983
406 (in isoform 1)	Ubiquitination	-	45.32	21906983
406 (in isoform 2)	Ubiquitination	-	45.32	21906983
406 (in isoform 3)	Ubiquitination	-	45.32	21906983
406 (in isoform 4)	Ubiquitination	-	45.32	21906983
406 (in isoform 5)	Ubiquitination	-	45.32	21906983
416	Methylation	AVEEMMDRIKKGVHL HHHHHHHHHHCCCCC	25.40	115916949
425	Ubiquitination	KKGVHLRPVNQTARP HCCCCCCCCCCCCCC	35.45	29967540
425 (in isoform 5)	Ubiquitination	-	35.45	21906983
433	Phosphorylation	VNQTARPKTKPESSK CCCCCCCCCCCCCCC	64.72	32142685
436 (in isoform 6)	Ubiquitination	-	52.51	21906983
439	Phosphorylation	PKTKPESSKGCESAV CCCCCCCCCCHHHHH	31.25	21964256
441	Phosphorylation	TKPESSKGCESAVDE CCCCCCCCHHHHHHH	23.52	32142685
446	Phosphorylation	SKGCESAVDELKGIL CCCHHHHHHHHHHHH	9.05	32142685
450	Ubiquitination	ESAVDELKGILGTLN HHHHHHHHHHHHHCC	41.69	29967540
455	Phosphorylation	ELKGILGTLNKSTSS HHHHHHHHCCCCCCC	24.58	23312004
455 (in isoform 6)	Ubiquitination	-	24.58	21906983
458	Ubiquitination	GILGTLNKSTSSRSL HHHHHCCCCCCCCCH	59.22	27667366
459	Phosphorylation	ILGTLNKSTSSRSLK HHHHCCCCCCCCCHH	32.23	28857561
460	Phosphorylation	LGTLNKSTSSRSLKS HHHCCCCCCCCCHHH	32.12	23312004
461	Phosphorylation	GTLNKSTSSRSLKSL HHCCCCCCCCCHHHC	30.74	23312004
462	Phosphorylation	TLNKSTSSRSLKSLD HCCCCCCCCCHHHCC	26.44	23312004
464	Phosphorylation	NKSTSSRSLKSLDPE CCCCCCCCHHHCCCC	41.94	26657352
464 (in isoform 5)	Ubiquitination	-	41.94	21906983
466	Ubiquitination	STSSRSLKSLDPENS CCCCCCHHHCCCCCC	50.66	21906983
466 (in isoform 1)	Ubiquitination	-	50.66	21906983
466 (in isoform 3)	Ubiquitination	-	50.66	21906983
466 (in isoform 4)	Ubiquitination	-	50.66	21906983
467	Phosphorylation	TSSRSLKSLDPENSE CCCCCHHHCCCCCCH	43.21	29255136
473	Phosphorylation	KSLDPENSETELERI HHCCCCCCHHHHHHH	44.21	25159151
475	Phosphorylation	LDPENSETELERILR CCCCCCHHHHHHHHH	45.77	30206219
485	Ubiquitination	ERILRRRKVTAEADS HHHHHHCCCCCCCCC	41.97	21906983
485 (in isoform 1)	Ubiquitination	-	41.97	21906983
485 (in isoform 3)	Ubiquitination	-	41.97	21906983
485 (in isoform 4)	Ubiquitination	-	41.97	21906983
487	Phosphorylation	ILRRRKVTAEADSSS HHHHCCCCCCCCCCC	23.09	27273156
492	Phosphorylation	KVTAEADSSSPTGIL CCCCCCCCCCCCEEE	39.50	30266825
493	Phosphorylation	VTAEADSSSPTGILA CCCCCCCCCCCEEEE	40.67	30266825
493 (in isoform 3)	Phosphorylation	-	40.67	21406692
494	Phosphorylation	TAEADSSSPTGILAT CCCCCCCCCCEEEEE	30.36	19664994
494 (in isoform 6)	Ubiquitination	-	30.36	21906983
496	Phosphorylation	EADSSSPTGILATSE CCCCCCCCEEEEECC	38.51	30266825
501	Phosphorylation	SPTGILATSESKSMP CCCEEEEECCCCCCC	29.58	30175587
502	Phosphorylation	PTGILATSESKSMPV CCEEEEECCCCCCCC	33.98	23403867
504	Phosphorylation	GILATSESKSMPVLG EEEEECCCCCCCCEE	29.78	23403867
506	Phosphorylation	LATSESKSMPVLGSV EEECCCCCCCCEECH	37.28	29255136
506 (in isoform 3)	Phosphorylation	-	37.28	21406692
512	Phosphorylation	KSMPVLGSVSSVTKT CCCCCEECHHHHHHH	18.05	23927012
514	Phosphorylation	MPVLGSVSSVTKTAL CCCEECHHHHHHHHH	22.31	25159151
515	Phosphorylation	PVLGSVSSVTKTALN CCEECHHHHHHHHHC	31.48	23927012
517	Phosphorylation	LGSVSSVTKTALNKK EECHHHHHHHHHCCC	25.32	23927012
519	Phosphorylation	SVSSVTKTALNKKTL CHHHHHHHHHCCCCC	27.32	22210691
524	Ubiquitination	TKTALNKKTLEAEFN HHHHHCCCCCEECCC	58.21	21906983
524 (in isoform 1)	Ubiquitination	-	58.21	21906983
524 (in isoform 3)	Ubiquitination	-	58.21	21906983
524 (in isoform 4)	Ubiquitination	-	58.21	21906983
525	Phosphorylation	KTALNKKTLEAEFNS HHHHCCCCCEECCCC	31.29	21815630
532	Phosphorylation	TLEAEFNSPSPPTPE CCEECCCCCCCCCCC	31.88	25159151
534	Phosphorylation	EAEFNSPSPPTPEPG EECCCCCCCCCCCCC	43.25	30278072
537	Phosphorylation	FNSPSPPTPEPGEGP CCCCCCCCCCCCCCC	43.60	28355574
556	Phosphorylation	GCTSSKVTFQPPSSI CCCCCCEEECCCCCC	21.93	23312004
556 (in isoform 5)	Ubiquitination	-	21.93	21906983
561	Phosphorylation	KVTFQPPSSIGCRKK CEEECCCCCCCCCHH	41.07	25159151
562	Phosphorylation	VTFQPPSSIGCRKKY EEECCCCCCCCCHHC	28.86	25159151
586	Phosphorylation	VVVLDPVSTHEPQTK EEEECCCCCCCCCCH	29.45	28348404
586 (in isoform 6)	Ubiquitination	-	29.45	21906983
587	Phosphorylation	VVLDPVSTHEPQTKD EEECCCCCCCCCCHH	30.41	28348404
599	Acetylation	TKDQVAEKDPTQHKE CHHHHCCCCCCCCCC	60.31	26051181
616	Ubiquitination	GEIQPENKEDSIENV CCCCCCCHHHHHHHH	62.33	2190698
616 (in isoform 1)	Ubiquitination	-	62.33	21906983
619	Phosphorylation	QPENKEDSIENVRET CCCCHHHHHHHHHHH	32.66	25159151
626	Phosphorylation	SIENVRETDSSNC-- HHHHHHHHCCCCC--	30.53	29449344
628	Phosphorylation	ENVRETDSSNC---- HHHHHHCCCCC----	30.76	25627689
629	Phosphorylation	NVRETDSSNC----- HHHHHCCCCC-----	45.05	25627689

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
101	S	Phosphorylation	Kinase	PAK1	Q13153	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
101	S	Phosphorylation	-
Phosphorylated on Ser-101 and Ser-249 by PAK1 through a CDC42- and RAC1-dependent signaling pathway, which enhances its association with F-actin retrograde flow in filopodia and lamellipodia of axonal growth cones.
101	S	Phosphorylation	-
Phosphorylation on Ser-101 and Ser-249 is increased by netrin-1.
249	S	Phosphorylation	20068231
Phosphorylated on Ser-101 and Ser-249 by PAK1 through a CDC42- and RAC1-dependent signaling pathway, which enhances its association with F-actin retrograde flow in filopodia and lamellipodia of axonal growth cones.
249	S	Phosphorylation	20068231
Phosphorylation on Ser-101 and Ser-249 is increased by netrin-1.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SHOT1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
UBXN1_HUMAN	UBXN1	physical	22939629
UNK_HUMAN	UNK	physical	22939629
CAP1_HUMAN	CAP1	physical	22863883
FAS_HUMAN	FASN	physical	22863883
ENPL_HUMAN	HSP90B1	physical	22863883
KCD12_HUMAN	KCTD12	physical	22863883
TPM4_HUMAN	TPM4	physical	22863883
SHOT1_HUMAN	KIAA1598	physical	25416956

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SHOT1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-506, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-494 AND THR-496, ANDMASS SPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-494; SER-506; SER-515;SER-532 AND THR-537, AND MASS SPECTROMETRY.	18669648 [Abstract]
"A probability-based approach for high-throughput proteinphosphorylation analysis and site localization."; Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.; Nat. Biotechnol. 24:1285-1292(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-537, AND MASSSPECTROMETRY.	16964243 [Abstract]

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