dbPTM

ARHG7_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ARHG7_HUMAN
UniProt AC	Q14155
Protein Name	Rho guanine nucleotide exchange factor 7
Gene Name	ARHGEF7
Organism	Homo sapiens (Human).
Sequence Length	803
Subcellular Localization	Cell junction, focal adhesion. Cell projection, ruffle. Cytoplasm, cell cortex. Cell projection, lamellipodium. Detected at cell adhesions. A small proportion is detected at focal adhesions.
Protein Description	Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons..
Protein Sequence	MNSAEQTVTWLITLGVLESPKKTISDPEGFLQASLKDGVVLCRLLERLLPGTIEKVYPEPRSESECLSNIREFLRGCGASLRLELLFPPSQPPQHLVTTILLSASTFDANDLYQGQNFNKVLSSLVTLNKVTADIGLGSDSVCARPSSHRIKSFDSLGSQSLHTRTSKLFQGQYRSLDMTDNSNNQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTLNGRTGWFPSNYVREVKASEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETENEYSKELQTVLSTYLRPLQTSEKLSSANISYLMGNLEEICSFQQMLVQSLEECTKLPEAQQRVGGCFLNLMPQMKTLYLTYCANHPSAVNVLTEHSEELGEFMETKGASSPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYHTDRQDIQKSMAAFKNLSAQCQEVRKRKELELQILTEAIRNWEGDDIKTLGNVTYMSQVLIQCAGSEEKNERYLLLFPNVLLMLSASPRMSGFIYQGKLPTTGMTITKLEDSENHRNAFEISGSMIERILVSCNNQQDLQEWVEHLQKQTKVTSVGNPTIKPHSVPSHTLPSHPVTPSSKHADSKPAPLTPAYHTLPHPSHHGTPHTTINWGPLEPPKTPKPWSLSCLRPAPPLRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFASRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLMHNHVLADDDQPSLDSLGRRSSLSRLEPSDLSEDSDYDSIWTAHSYRMGSTSRKSCCSYISHQN

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1 (in isoform 1)	Acetylation	-	8.91	22814378
1 (in isoform 6)	Acetylation	-	8.91	22814378
2 (in isoform 1)	Acetylation	-	50.34	22223895
2 (in isoform 6)	Acetylation	-	50.34	22223895
3	Phosphorylation	-----MNSAEQTVTW -----CCHHHHHHHH	31.70	23663014
7	Phosphorylation	-MNSAEQTVTWLITL -CCHHHHHHHHHHHH	16.06	23663014
9	Phosphorylation	NSAEQTVTWLITLGV CHHHHHHHHHHHHCH	20.24	23663014
13	Phosphorylation	QTVTWLITLGVLESP HHHHHHHHHCHHCCC	18.45	23663014
19	Phosphorylation	ITLGVLESPKKTISD HHHCHHCCCCCCCCC	37.55	23663014
23	Phosphorylation	VLESPKKTISDPEGF HHCCCCCCCCCCCCH	31.66	26699800
25	Phosphorylation	ESPKKTISDPEGFLQ CCCCCCCCCCCCHHH	52.96	26657352
62	Phosphorylation	KVYPEPRSESECLSN HHCCCCCCHHHHHHH	57.07	27732954
64	Phosphorylation	YPEPRSESECLSNIR CCCCCCHHHHHHHHH	34.55	27732954
123	Phosphorylation	QNFNKVLSSLVTLNK CCHHHHHHHHHHHCC	25.31	23403867
124	Phosphorylation	NFNKVLSSLVTLNKV CHHHHHHHHHHHCCE	23.77	23403867
127	Phosphorylation	KVLSSLVTLNKVTAD HHHHHHHHHCCEECC	29.88	-
139	Phosphorylation	TADIGLGSDSVCARP ECCCCCCCCCCCCCC	31.58	23403867
141	Phosphorylation	DIGLGSDSVCARPSS CCCCCCCCCCCCCCC	22.31	23403867
147	Phosphorylation	DSVCARPSSHRIKSF CCCCCCCCCCCCCCH	32.97	23403867
148	Phosphorylation	SVCARPSSHRIKSFD CCCCCCCCCCCCCHH	21.33	23403867
153	Phosphorylation	PSSHRIKSFDSLGSQ CCCCCCCCHHHCCCC	31.73	23911959
156	Phosphorylation	HRIKSFDSLGSQSLH CCCCCHHHCCCCCCH	32.40	23403867
159	Phosphorylation	KSFDSLGSQSLHTRT CCHHHCCCCCCHHHH	23.14	23403867
161	Phosphorylation	FDSLGSQSLHTRTSK HHHCCCCCCHHHHHH	24.66	23403867
166	Phosphorylation	SQSLHTRTSKLFQGQ CCCCHHHHHHHHCCC	31.22	28857561
167	Phosphorylation	QSLHTRTSKLFQGQY CCCHHHHHHHHCCCC	24.87	28857561
174	Phosphorylation	SKLFQGQYRSLDMTD HHHHCCCCCCCCCCC	14.70	24117733
176	Phosphorylation	LFQGQYRSLDMTDNS HHCCCCCCCCCCCCC	24.64	26846344
180	Phosphorylation	QYRSLDMTDNSNNQL CCCCCCCCCCCCCEE	31.53	23403867
183	Phosphorylation	SLDMTDNSNNQLVVR CCCCCCCCCCEEEEE	39.71	24117733
204	Phosphorylation	QTNEDELSFSKGDVI CCCCCCCCCCCCCEE	25.98	24719451
225	Phosphorylation	EGGWWEGTLNGRTGW ECCEEEEEECCCCCE	12.92	24076635
242	Ubiquitination	SNYVREVKASEKPVS HHHEEEEECCCCCCC	41.25	21906983
244	Phosphorylation	YVREVKASEKPVSPK HEEEEECCCCCCCCC	40.20	23403867
249	Phosphorylation	KASEKPVSPKSGTLK ECCCCCCCCCCCCCC	34.83	28355574
251	Ubiquitination	SEKPVSPKSGTLKSP CCCCCCCCCCCCCCC	54.75	2190698
252	Phosphorylation	EKPVSPKSGTLKSPP CCCCCCCCCCCCCCC	40.15	23927012
254	Phosphorylation	PVSPKSGTLKSPPKG CCCCCCCCCCCCCCC	37.80	23927012
257	Phosphorylation	PKSGTLKSPPKGFDT CCCCCCCCCCCCCCC	49.93	23927012
264	Phosphorylation	SPPKGFDTTAINKSY CCCCCCCCCCCCHHH	18.83	23927012
265	Phosphorylation	PPKGFDTTAINKSYY CCCCCCCCCCCHHHH	27.64	23927012
270	Phosphorylation	DTTAINKSYYNVVLQ CCCCCCHHHHHHHHH	28.50	22199227
271	Phosphorylation	TTAINKSYYNVVLQN CCCCCHHHHHHHHHH	10.73	22199227
272	Phosphorylation	TAINKSYYNVVLQNI CCCCHHHHHHHHHHH	14.47	22199227
295	Phosphorylation	KELQTVLSTYLRPLQ HHHHHHHHHHHCCCC	15.63	28857561
296	Phosphorylation	ELQTVLSTYLRPLQT HHHHHHHHHHCCCCC	23.55	28857561
297	Phosphorylation	LQTVLSTYLRPLQTS HHHHHHHHHCCCCCC	9.42	28857561
312 (in isoform 5)	Ubiquitination	-	2.61	-
325 (in isoform 5)	Ubiquitination	-	5.18	-
349 (in isoform 5)	Ubiquitination	-	2.63	-
392	Phosphorylation	FMETKGASSPGILVL HHHHCCCCCCCEEEE	45.81	28348404
393	Phosphorylation	METKGASSPGILVLT HHHCCCCCCCEEEEE	26.98	20068231
400	Phosphorylation	SPGILVLTTGLSKPF CCCEEEEECCCCCCC	16.02	20068231
401	Phosphorylation	PGILVLTTGLSKPFM CCEEEEECCCCCCCC	31.78	20068231
404	Phosphorylation	LVLTTGLSKPFMRLD EEEECCCCCCCCHHH	39.36	20068231
427	Phosphorylation	LERHMEDYHTDRQDI HHHHHHHHCCCHHHH	8.04	-
512	Phosphorylation	PNVLLMLSASPRMSG CCHHHHHCCCCCCCC	16.85	29514088
514	Phosphorylation	VLLMLSASPRMSGFI HHHHHCCCCCCCCCE	15.06	26074081
516	Phosphorylation	LMLSASPRMSGFIYQ HHHCCCCCCCCCEEC	29.42	18184567
516 (in isoform 1)	Phosphorylation	-	29.42	15513924
518	Phosphorylation	LSASPRMSGFIYQGK HCCCCCCCCCEECCC	31.65	19664994
522	Phosphorylation	PRMSGFIYQGKLPTT CCCCCCEECCCCCCC	14.67	30266825
526 (in isoform 1)	Phosphorylation	-	6.11	15513924
528	Phosphorylation	IYQGKLPTTGMTITK EECCCCCCCCEEEEE	46.55	23403867
529	Phosphorylation	YQGKLPTTGMTITKL ECCCCCCCCEEEEEC	24.02	23403867
531	Sulfoxidation	GKLPTTGMTITKLED CCCCCCCEEEEECCC	1.99	21406390
532	Phosphorylation	KLPTTGMTITKLEDS CCCCCCEEEEECCCC	27.17	28464451
534	Phosphorylation	PTTGMTITKLEDSEN CCCCEEEEECCCCCC	22.02	23403867
539	Phosphorylation	TITKLEDSENHRNAF EEEECCCCCCCCCCC	30.81	23927012
549	Phosphorylation	HRNAFEISGSMIERI CCCCCHHCHHHHHHH	20.02	27251275
551	Phosphorylation	NAFEISGSMIERILV CCCHHCHHHHHHHHH	15.14	28857561
551 (in isoform 1)	Phosphorylation	-	15.14	16674116
552	Sulfoxidation	AFEISGSMIERILVS CCHHCHHHHHHHHHH	4.15	21406390
554 (in isoform 1)	Phosphorylation	-	25.65	24719451
560 (in isoform 1)	Phosphorylation	-	4.29	30266825
577 (in isoform 1)	Phosphorylation	-	34.22	26657352
579 (in isoform 1)	Phosphorylation	-	5.68	30266825
580	Phosphorylation	LQKQTKVTSVGNPTI HHHHCCEEECCCCCC	21.17	23403867
581	Phosphorylation	QKQTKVTSVGNPTIK HHHCCEEECCCCCCC	30.89	23403867
583 (in isoform 1)	Phosphorylation	-	33.88	30266825
586	Phosphorylation	VTSVGNPTIKPHSVP EEECCCCCCCCCCCC	45.71	23403867
589 (in isoform 1)	Phosphorylation	-	22.57	26356563
591	Phosphorylation	NPTIKPHSVPSHTLP CCCCCCCCCCCCCCC	44.22	23403867
593 (in isoform 1)	Phosphorylation	-	24.33	26356563
594	Phosphorylation	IKPHSVPSHTLPSHP CCCCCCCCCCCCCCC	27.27	23403867
595 (in isoform 1)	Phosphorylation	-	25.92	28796482
596	Phosphorylation	PHSVPSHTLPSHPVT CCCCCCCCCCCCCCC	44.71	23403867
599	Phosphorylation	VPSHTLPSHPVTPSS CCCCCCCCCCCCCCC	43.46	23403867
603	Phosphorylation	TLPSHPVTPSSKHAD CCCCCCCCCCCCCCC	23.15	25159151
605	Phosphorylation	PSHPVTPSSKHADSK CCCCCCCCCCCCCCC	42.53	28152594
606	Phosphorylation	SHPVTPSSKHADSKP CCCCCCCCCCCCCCC	29.66	28152594
609 (in isoform 5)	Ubiquitination	-	26.89	-
614 (in isoform 1)	Phosphorylation	-	18.57	29743597
617	Phosphorylation	DSKPAPLTPAYHTLP CCCCCCCCCCCCCCC	12.57	28796482
620	Phosphorylation	PAPLTPAYHTLPHPS CCCCCCCCCCCCCCC	9.13	28796482
622	Phosphorylation	PLTPAYHTLPHPSHH CCCCCCCCCCCCCCC	29.34	28796482
632 (in isoform 5)	Ubiquitination	-	27.67	-
636 (in isoform 5)	Phosphorylation	-	4.25	16674116
639 (in isoform 5)	Phosphorylation	-	16.66	24719451
643 (in isoform 5)	Ubiquitination	-	43.29	-
645 (in isoform 5)	Phosphorylation	-	84.15	30266825
653	Phosphorylation	TPKPWSLSCLRPAPP CCCCCCCCCCCCCCC	12.94	18491316
656 (in isoform 5)	Ubiquitination	-	29.66	-
662 (in isoform 5)	Phosphorylation	-	28.97	26657352
663 (in isoform 5)	Ubiquitination	-	35.68	-
664	O-linked_Glycosylation	PAPPLRPSAALCYKE CCCCCCCCEEEECCH	21.53	25367160
664 (in isoform 5)	Phosphorylation	-	21.53	30266825
668 (in isoform 5)	Phosphorylation	-	3.80	30266825
673 (in isoform 1)	Ubiquitination	-	7.92	21906983
674	Phosphorylation	LCYKEDLSKSPKTMK EECCHHHCCCHHHHH	43.33	30266825
674 (in isoform 5)	Phosphorylation	-	43.33	26356563
675	Acetylation	CYKEDLSKSPKTMKK ECCHHHCCCHHHHHH	77.42	7695211
676	Phosphorylation	YKEDLSKSPKTMKKL CCHHHCCCHHHHHHH	28.40	30266825
678	Acetylation	EDLSKSPKTMKKLLP HHHCCCHHHHHHHCC	69.23	7695223
678 (in isoform 5)	Phosphorylation	-	69.23	26356563
679	Phosphorylation	DLSKSPKTMKKLLPK HHCCCHHHHHHHCCC	37.29	26074081
680 (in isoform 5)	Phosphorylation	-	4.50	28796482
681	Acetylation	SKSPKTMKKLLPKRK CCCHHHHHHHCCCCC	45.56	30587019
682	Acetylation	KSPKTMKKLLPKRKP CCHHHHHHHCCCCCC	44.42	7695235
683 (in isoform 5)	Ubiquitination	-	9.15	21906983
694	Phosphorylation	RKPERKPSDEEFASR CCCCCCCCHHHHHCH	61.77	29255136
699 (in isoform 5)	Phosphorylation	-	16.23	29743597
700	Phosphorylation	PSDEEFASRKSTAAL CCHHHHHCHHHHHHH	45.63	23927012
702	Ubiquitination	DEEFASRKSTAALEE HHHHHCHHHHHHHHH	50.06	-
703	Phosphorylation	EEFASRKSTAALEED HHHHCHHHHHHHHHH	23.36	29255136
704	Phosphorylation	EFASRKSTAALEEDA HHHCHHHHHHHHHHH	20.98	29255136
715	Ubiquitination	EEDAQILKVIEAYCT HHHHHHHHHHHHHHC	42.68	-
720	Phosphorylation	ILKVIEAYCTSAKTR HHHHHHHHHCCCCHH	5.40	26552605
722	Phosphorylation	KVIEAYCTSAKTRQT HHHHHHHCCCCHHHH	21.02	26552605
723	Phosphorylation	VIEAYCTSAKTRQTL HHHHHHCCCCHHHHH	24.98	26552605
725	Acetylation	EAYCTSAKTRQTLNS HHHHCCCCHHHHHHC	43.96	25953088
760	Phosphorylation	LDSLGRRSSLSRLEP HHHHCCCCCCCCCCC	33.98	30576142
761	Phosphorylation	DSLGRRSSLSRLEPS HHHCCCCCCCCCCCC	28.66	20068231
763	Phosphorylation	LGRRSSLSRLEPSDL HCCCCCCCCCCCCCC	36.45	30576142
771	Phosphorylation	RLEPSDLSEDSDYDS CCCCCCCCCCCCCCC	44.33	25921289
774	Phosphorylation	PSDLSEDSDYDSIWT CCCCCCCCCCCCCHH	33.74	25921289
776	Phosphorylation	DLSEDSDYDSIWTAH CCCCCCCCCCCHHHH	18.62	30576142
784	Phosphorylation	DSIWTAHSYRMGSTS CCCHHHHHHCCCCCC	16.39	-
785	Phosphorylation	SIWTAHSYRMGSTSR CCHHHHHHCCCCCCC	8.51	-
794	Phosphorylation	MGSTSRKSCCSYISH CCCCCCHHHHHHHHC	21.17	27732954
797	Phosphorylation	TSRKSCCSYISHQN- CCCHHHHHHHHCCC-	29.81	27732954
800	Phosphorylation	KSCCSYISHQN---- HHHHHHHHCCC----	15.70	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
516	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
516	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
518	S	Phosphorylation	Kinase	PRKCG	P05129	GPS
526	T	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
526	T	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
620	Y	Phosphorylation	Kinase	PTK2	P34152-2	GPS
694	S	Phosphorylation	Kinase	CAMK1	Q14012	Uniprot
761	S	Phosphorylation	Kinase	PRKCG	P05129	GPS
-	K	Ubiquitination	E3 ubiquitin ligase	CBL	P22681	PMID:16407834

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
694	S	Phosphorylation		18184567
Phosphorylated on Ser-694 by CaMK1; enhancement of GEF activity and downstream activation of RAC1.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ARHG7_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SHAN2_HUMAN	SHANK2	physical	12626503
SHAN1_HUMAN	SHANK1	physical	12626503
CBL_HUMAN	CBL	physical	12935897
SHAN3_HUMAN	SHANK3	physical	12626503
CBL_HUMAN	CBL	physical	16407834
PAK1_HUMAN	PAK1	physical	16407834
CBL_HUMAN	CBL	physical	14505571
CBLB_HUMAN	CBLB	physical	16228008
CBL_HUMAN	CBL	physical	22588125
ITCH_HUMAN	ITCH	physical	17652093
LRRK2_HUMAN	LRRK2	physical	21048939
CBL_HUMAN	CBL	physical	23175185
KDM2A_HUMAN	KDM2A	physical	26344197
ARHG6_HUMAN	ARHGEF6	physical	28514442
GIT1_HUMAN	GIT1	physical	28514442
PAK1_HUMAN	PAK1	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ARHG7_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176 AND SER-518, ANDMASS SPECTROMETRY.	19690332 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-694, AND MASSSPECTROMETRY.	18669648 [Abstract]
"Activity-dependent synaptogenesis: regulation by a CaM-kinasekinase/CaM-kinase I/betaPIX signaling complex."; Saneyoshi T., Wayman G., Fortin D., Davare M., Hoshi N., Nozaki N.,Natsume T., Soderling T.R.; Neuron 57:94-107(2008). Cited for: FUNCTION, PHOSPHORYLATION AT SER-694, AND INTERACTION WITH CAMK1.	18184567 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-249 AND SER-518, ANDMASS SPECTROMETRY.	17081983 [Abstract]

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