dbPTM

AFAP1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	AFAP1_HUMAN
UniProt AC	Q8N556
Protein Name	Actin filament-associated protein 1
Gene Name	AFAP1
Organism	Homo sapiens (Human).
Sequence Length	730
Subcellular Localization	Cytoplasm, cytoskeleton . Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles upon their induction.
Protein Description	Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells..
Protein Sequence	MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCNITYIPKDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDSECPPPPSSPVHKAELEKKLSSERPSSDGEGVVENGITTCNGKEQVKRKKSSKSEAKGTVSKVTGKKITKIISLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDNKLIFHKDRTDLKTHIVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPEALHYDYIDVEMSASVIQTAKQTFCFMNRRVISANPYLGGTSNGYAHPSGTALHYDDVPCINGSLKGKKPPVASNGVTGKGKTLSSQPKKADPAAVVKRTGSNAAQYKYGKNRVEADAKRLQTKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKPQAILEEKLKQLEEECRQKEAERVSLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPISSCDTSDTEGPVPVNSAAVLKKSQAAPGSSPCRGHVLRKAKEWELKNGT

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEELIVEL -------CHHHHHHH	9.01	22814378
19	Phosphorylation	LELLDHEYLTSTVRE HHHHCHHHHHCHHHH	16.32	68854629
42	Ubiquitination	LLRIQSSKGFDVKDH HHHHHHCCCCCCCHH	69.69	-
47	Ubiquitination	SSKGFDVKDHAQKQE HCCCCCCCHHHHHHH	45.71	-
93	Phosphorylation	TSSLPEGYYEEAVPL CCCCCCCCCCCCCCC	13.17	9655255
94	Phosphorylation	SSLPEGYYEEAVPLS CCCCCCCCCCCCCCC	20.39	9655255
101	Phosphorylation	YEEAVPLSPGKAPEY CCCCCCCCCCCCCCH	26.07	80868205
119	Phosphorylation	NYDSDAMSSSYESYD CCCCCCCCCCCCCCC	20.18	27251275
120	Phosphorylation	YDSDAMSSSYESYDE CCCCCCCCCCCCCCC	24.69	27251275
121	Phosphorylation	DSDAMSSSYESYDEE CCCCCCCCCCCCCCC	26.13	27251275
122	Phosphorylation	SDAMSSSYESYDEEE CCCCCCCCCCCCCCC	15.73	27251275
124	Phosphorylation	AMSSSYESYDEEEED CCCCCCCCCCCCCCC	29.65	27251275
125	Phosphorylation	MSSSYESYDEEEEDG CCCCCCCCCCCCCCC	18.55	9655255
147	Phosphorylation	QWPSEEASMDLVKDA CCCCHHHCCHHHHHH	18.99	28387310
155	Acetylation	MDLVKDAKICAFLLR CHHHHHHHHHHHHHH	48.75	26051181
171	Acetylation	KRFGQWTKLLCVIKD HHHCCCEEEEEEEEC	36.18	26051181
177	Acetylation	TKLLCVIKDTKLLCY EEEEEEEECCEEEEE	38.66	26051181
180	Acetylation	LCVIKDTKLLCYKSS EEEEECCEEEEEECC	49.98	26051181
208	Acetylation	CNITYIPKDSKKKKH CCEEEECCCCCCCCC	66.14	34677437
212	Acetylation	YIPKDSKKKKHELKI EECCCCCCCCCEEEE	71.63	34677445
220	Phosphorylation	KKHELKITQQGTDPL CCCEEEEEECCCCCE	17.16	20068231
224	Phosphorylation	LKITQQGTDPLVLAV EEEEECCCCCEEEEE	30.06	20068231
233	Phosphorylation	PLVLAVQSKEQAEQW CEEEEECCHHHHHHH	31.40	20068231
234	Ubiquitination	LVLAVQSKEQAEQWL EEEEECCHHHHHHHH	36.43	-
242	Acetylation	EQAEQWLKVIKEAYS HHHHHHHHHHHHHHC	38.67	26051181
242	Ubiquitination	EQAEQWLKVIKEAYS HHHHHHHHHHHHHHC	38.67	-
248	Phosphorylation	LKVIKEAYSGCSGPV HHHHHHHHCCCCCCC	13.49	24732914
249	Phosphorylation	KVIKEAYSGCSGPVD HHHHHHHCCCCCCCC	40.13	24732914
252	Phosphorylation	KEAYSGCSGPVDSEC HHHHCCCCCCCCCCC	50.05	24732914
257	Phosphorylation	GCSGPVDSECPPPPS CCCCCCCCCCCCCCC	41.13	23927012
264	Phosphorylation	SECPPPPSSPVHKAE CCCCCCCCCCCCHHH	53.71	22167270
265	Phosphorylation	ECPPPPSSPVHKAEL CCCCCCCCCCCHHHH	36.39	22167270
277	Phosphorylation	AELEKKLSSERPSSD HHHHHHHCCCCCCCC	38.75	29255136
278	Phosphorylation	ELEKKLSSERPSSDG HHHHHHCCCCCCCCC	48.32	30266825
282	Phosphorylation	KLSSERPSSDGEGVV HHCCCCCCCCCCCCC	47.48	29255136
283	Phosphorylation	LSSERPSSDGEGVVE HCCCCCCCCCCCCCC	52.93	29255136
294	Phosphorylation	GVVENGITTCNGKEQ CCCCCCCEECCCHHH	27.05	23403867
295	Phosphorylation	VVENGITTCNGKEQV CCCCCCEECCCHHHH	11.31	23403867
307	Phosphorylation	EQVKRKKSSKSEAKG HHHHCCCCCCCCCCC	46.14	26074081
308	Phosphorylation	QVKRKKSSKSEAKGT HHHCCCCCCCCCCCC	49.43	26074081
310	Phosphorylation	KRKKSSKSEAKGTVS HCCCCCCCCCCCCEE	44.74	26074081
315	Phosphorylation	SKSEAKGTVSKVTGK CCCCCCCCEEEHHCC	22.39	30576142
317	Phosphorylation	SEAKGTVSKVTGKKI CCCCCCEEEHHCCCH	22.65	26074081
320	Phosphorylation	KGTVSKVTGKKITKI CCCEEEHHCCCHHEE	47.20	26074081
329	Phosphorylation	KKITKIISLGKKKPS CCHHEEEECCCCCCC	34.24	20860994
336	Phosphorylation	SLGKKKPSTDEQTSS ECCCCCCCCCCCCCC	58.32	25463755
337	Phosphorylation	LGKKKPSTDEQTSSA CCCCCCCCCCCCCCC	52.67	25463755
341	Phosphorylation	KPSTDEQTSSAEEDV CCCCCCCCCCCHHCC	23.70	23927012
342	Phosphorylation	PSTDEQTSSAEEDVP CCCCCCCCCCHHCCC	27.52	25463755
343	Phosphorylation	STDEQTSSAEEDVPT CCCCCCCCCHHCCCC	42.99	25463755
350	Phosphorylation	SAEEDVPTCGYLNVL CCHHCCCCCCHHHHH	20.57	41242819
353	Phosphorylation	EDVPTCGYLNVLSNS HCCCCCCHHHHHCCC	9.39	23927012
358	Phosphorylation	CGYLNVLSNSRWRER CCHHHHHCCCCHHHH	28.60	24732914
360	Phosphorylation	YLNVLSNSRWRERWC HHHHHCCCCHHHHEE	29.82	24732914
373	Ubiquitination	WCRVKDNKLIFHKDR EEEEECCEEEEECCC	53.76	-
378	Malonylation	DNKLIFHKDRTDLKT CCEEEEECCCCCCCC	37.85	26320211
378	Ubiquitination	DNKLIFHKDRTDLKT CCEEEEECCCCCCCC	37.85	-
384	Ubiquitination	HKDRTDLKTHIVSIP ECCCCCCCCCEEEEE	40.65	-
389	Phosphorylation	DLKTHIVSIPLRGCE CCCCCEEEEECCCCE	20.20	24719451
424	Phosphorylation	EVAVLEASSSEDMGR EEEEEEECCCCHHHH	25.64	27732954
425	Phosphorylation	VAVLEASSSEDMGRW EEEEEECCCCHHHHH	44.81	27732954
426	Phosphorylation	AVLEASSSEDMGRWI EEEEECCCCHHHHHH	34.81	27732954
451	Phosphorylation	TDPEALHYDYIDVEM CCHHHHCCCEEEHHH	15.99	131049
453	Phosphorylation	PEALHYDYIDVEMSA HHHHCCCEEEHHHCH	7.58	9655255
483	Phosphorylation	RVISANPYLGGTSNG EEEECCCCCCCCCCC	20.13	26356563
487	Phosphorylation	ANPYLGGTSNGYAHP CCCCCCCCCCCCCCC	19.74	26356563
488	Phosphorylation	NPYLGGTSNGYAHPS CCCCCCCCCCCCCCC	31.37	26356563
491	Phosphorylation	LGGTSNGYAHPSGTA CCCCCCCCCCCCCCC	13.14	119545
495	Phosphorylation	SNGYAHPSGTALHYD CCCCCCCCCCCCCCC	38.62	26356563
497	Phosphorylation	GYAHPSGTALHYDDV CCCCCCCCCCCCCCC	30.31	26356563
501	Phosphorylation	PSGTALHYDDVPCIN CCCCCCCCCCCCCCC	18.06	26356563
510	Phosphorylation	DVPCINGSLKGKKPP CCCCCCCCCCCCCCC	23.55	26356563
512	Acetylation	PCINGSLKGKKPPVA CCCCCCCCCCCCCCC	71.08	26051181
520	Phosphorylation	GKKPPVASNGVTGKG CCCCCCCCCCCCCCC	34.06	46160037
524	Phosphorylation	PVASNGVTGKGKTLS CCCCCCCCCCCCCCC	34.28	72840035
528	Ubiquitination	NGVTGKGKTLSSQPK CCCCCCCCCCCCCCC	50.18	-
529	Phosphorylation	GVTGKGKTLSSQPKK CCCCCCCCCCCCCCC	40.31	23403867
531	Phosphorylation	TGKGKTLSSQPKKAD CCCCCCCCCCCCCCC	31.96	23403867
532	Phosphorylation	GKGKTLSSQPKKADP CCCCCCCCCCCCCCH	54.32	23403867
537	Phosphorylation	LSSQPKKADPAAVVK CCCCCCCCCHHHHEE	33.86	-
537 (in isoform 2)	Phosphorylation	-	33.86	25884760
546	Phosphorylation	PAAVVKRTGSNAAQY HHHHEECCCCCHHHH	38.96	25159151
548	Phosphorylation	AVVKRTGSNAAQYKY HHEECCCCCHHHHHH	23.76	28355574
553	Phosphorylation	TGSNAAQYKYGKNRV CCCCHHHHHHCCCHH	11.05	30835073
554	Ubiquitination	GSNAAQYKYGKNRVE CCCHHHHHHCCCHHH	35.13	-
555	Phosphorylation	SNAAQYKYGKNRVEA CCHHHHHHCCCHHHH	28.53	23312004
570	Ubiquitination	DAKRLQTKEEELLKR HHHHHHHHHHHHHHH	50.60	-
606	Ubiquitination	IEVNAGRKPQAILEE HHHCCCCCHHHHHHH	40.33	-
614	Ubiquitination	PQAILEEKLKQLEEE HHHHHHHHHHHHHHH	52.16	-
631	Phosphorylation	QKEAERVSLELELTE HHHHHHHHHHEEHHH	23.50	46160043
632	Phosphorylation	KEAERVSLELELTEV HHHHHHHHHEEHHHH	8.78	24719451
637	Phosphorylation	VSLELELTEVKESLK HHHHEEHHHHHHHHH	29.38	46160055
652	Phosphorylation	KALAGGVTLGLAIEP HHHHCCCEEEEEECC	20.55	22964224
661	Phosphorylation	GLAIEPKSGTSSPQS EEEECCCCCCCCCCC	59.00	22167270
663	Phosphorylation	AIEPKSGTSSPQSPV EECCCCCCCCCCCCE	32.87	22167270
664	Phosphorylation	IEPKSGTSSPQSPVF ECCCCCCCCCCCCEE	43.47	22167270
665	Phosphorylation	EPKSGTSSPQSPVFR CCCCCCCCCCCCEEC	27.14	22167270
668	Phosphorylation	SGTSSPQSPVFRHRT CCCCCCCCCEECCCC	26.81	19664994
675	Phosphorylation	SPVFRHRTLENSPIS CCEECCCCCCCCCCC	32.09	9497717
679	Phosphorylation	RHRTLENSPISSCDT CCCCCCCCCCCCCCC	17.78	23927012
682	Phosphorylation	TLENSPISSCDTSDT CCCCCCCCCCCCCCC	28.44	25159151
683	Phosphorylation	LENSPISSCDTSDTE CCCCCCCCCCCCCCC	19.41	23927012
686	Phosphorylation	SPISSCDTSDTEGPV CCCCCCCCCCCCCCC	32.59	30278072
687	Phosphorylation	PISSCDTSDTEGPVP CCCCCCCCCCCCCCC	29.32	30278072
689	Phosphorylation	SSCDTSDTEGPVPVN CCCCCCCCCCCCCCC	42.48	30278072
697	Phosphorylation	EGPVPVNSAAVLKKS CCCCCCCHHHHHHHH	20.17	23403867
704	Phosphorylation	SAAVLKKSQAAPGSS HHHHHHHHHCCCCCC	24.38	24732914
710	Phosphorylation	KSQAAPGSSPCRGHV HHHCCCCCCCCCHHH	30.01	25159151
711	Phosphorylation	SQAAPGSSPCRGHVL HHCCCCCCCCCHHHH	33.65	25159151
730	Phosphorylation	EWELKNGT------- HHHCCCCC-------	44.84	21712546
748	Phosphorylation	------------------------- -------------------------		24719451
749	Phosphorylation	-------------------------- --------------------------		24719451
752	Phosphorylation	----------------------------- -----------------------------		24719451
763	Phosphorylation	---------------------------------------- ----------------------------------------		24719451
766	Phosphorylation	------------------------------------------- -------------------------------------------		27251275
770	Phosphorylation	----------------------------------------------- -----------------------------------------------		24719451
771	Phosphorylation	------------------------------------------------ ------------------------------------------------		27251275
794	Phosphorylation	----------------------------------------------------------------------- -----------------------------------------------------------------------		24719451
795	Phosphorylation	------------------------------------------------------------------------ ------------------------------------------------------------------------		27251275

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
93	Y	Phosphorylation	Kinase	SRC	P12931	PSP
94	Y	Phosphorylation	Kinase	SRC	P12931	PSP
125	Y	Phosphorylation	Kinase	SRC	P12931	PSP
277	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
451	Y	Phosphorylation	Kinase	SRC	P12931	PSP
453	Y	Phosphorylation	Kinase	SRC	P12931	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of AFAP1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of AFAP1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AFAP1_HUMAN	AFAP1	physical	9619827
ACTC_HUMAN	ACTC1	physical	12134071
KPCB_HUMAN	PRKCB	physical	12134071
KPCE_HUMAN	PRKCE	physical	12134071
KPCG_HUMAN	PRKCG	physical	12134071
KPCD_HUMAN	PRKCD	physical	12134071
KPCA_HUMAN	PRKCA	physical	12134071

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of AFAP1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-668, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-664; SER-668 ANDSER-687, AND MASS SPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-277; SER-278; SER-282;SER-283; THR-663; SER-664; SER-665; SER-668; SER-679; THR-686 ANDSER-687, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-668, AND MASSSPECTROMETRY.	18220336 [Abstract]
"Phosphoproteome analysis of the human mitotic spindle."; Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R.; Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-665 AND SER-668, ANDMASS SPECTROMETRY.	16565220 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-282; SER-336; THR-337;THR-341; SER-342 AND SER-343, AND MASS SPECTROMETRY.	17081983 [Abstract]