dbPTM

IASPP_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	IASPP_HUMAN
UniProt AC	Q8WUF5
Protein Name	RelA-associated inhibitor
Gene Name	PPP1R13L
Organism	Homo sapiens (Human).
Sequence Length	828
Subcellular Localization	Cytoplasm . Nucleus . Predominantly cytoplasmic but also nuclear.
Protein Description	Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis..
Protein Sequence	MDSEAFQSARDFLDMNFQSLAMKHMDLKQMELDTAAAKVDELTKQLESLWSDSPAPPGPQAGPPSRPPRYSSSSIPEPFGSRGSPRKAATDGADTPFGRSESAPTLHPYSPLSPKGRPSSPRTPLYLQPDAYGSLDRATSPRPRAFDGAGSSLGRAPSPRPGPGPLRQQGPPTPFDFLGRAGSPRGSPLAEGPQAFFPERGPSPRPPATAYDAPASAFGSSLLGSGGSAFAPPLRAQDDLTLRRRPPKAWNESDLDVAYEKKPSQTASYERLDVFARPASPSLQLLPWRESSLDGLGGTGKDNLTSATLPRNYKVSPLASDRRSDAGSYRRSLGSAGPSGTLPRSWQPVSRIPMPPSSPQPRGAPRQRPIPLSMIFKLQNAFWEHGASRAMLPGSPLFTRAPPPKLQPQPQPQPQPQSQPQPQLPPQPQTQPQTPTPAPQHPQQTWPPVNEGPPKPPTELEPEPEIEGLLTPVLEAGDVDEGPVARPLSPTRLQPALPPEAQSVPELEEVARVLAEIPRPLKRRGSMEQAPAVALPPTHKKQYQQIISRLFHRHGGPGPGGPEPELSPITEGSEARAGPPAPAPPAPIPPPAPSQSSPPEQPQSMEMRSVLRKAGSPRKARRARLNPLVLLLDAALTGELEVVQQAVKEMNDPSQPNEEGITALHNAICGANYSIVDFLITAGANVNSPDSHGWTPLHCAASCNDTVICMALVQHGAAIFATTLSDGATAFEKCDPYREGYADCATYLADVEQSMGLMNSGAVYALWDYSAEFGDELSFREGESVTVLRRDGPEETDWWWAALHGQEGYVPRNYFGLFPRVKPQRSKV

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MDSEAFQS -------CCHHHHHH	13.28	22814378
19	Phosphorylation	FLDMNFQSLAMKHMD HHHHHHHHHHHHHCC	17.60	28555341
28	Ubiquitination	AMKHMDLKQMELDTA HHHHCCHHHHHHHHH	43.25	-
43	Phosphorylation	AAKVDELTKQLESLW HHHHHHHHHHHHHHH	18.07	24719451
48	Phosphorylation	ELTKQLESLWSDSPA HHHHHHHHHHCCCCC	43.67	24719451
51	Phosphorylation	KQLESLWSDSPAPPG HHHHHHHCCCCCCCC	33.24	22199227
53	Phosphorylation	LESLWSDSPAPPGPQ HHHHHCCCCCCCCCC	19.90	22199227
65	Phosphorylation	GPQAGPPSRPPRYSS CCCCCCCCCCCCCCC	61.63	24719451
70	Phosphorylation	PPSRPPRYSSSSIPE CCCCCCCCCCCCCCC	20.76	28985074
71	Phosphorylation	PSRPPRYSSSSIPEP CCCCCCCCCCCCCCC	25.64	25159151
72	Phosphorylation	SRPPRYSSSSIPEPF CCCCCCCCCCCCCCC	21.12	30278072
73	Phosphorylation	RPPRYSSSSIPEPFG CCCCCCCCCCCCCCC	26.75	30278072
74	Phosphorylation	PPRYSSSSIPEPFGS CCCCCCCCCCCCCCC	44.07	30278072
81	Phosphorylation	SIPEPFGSRGSPRKA CCCCCCCCCCCCCCC	33.57	22167270
82	Methylation	IPEPFGSRGSPRKAA CCCCCCCCCCCCCCC	50.41	54558243
84	Phosphorylation	EPFGSRGSPRKAATD CCCCCCCCCCCCCCC	22.03	22167270
87	Methylation	GSRGSPRKAATDGAD CCCCCCCCCCCCCCC	45.84	116253281
87	Ubiquitination	GSRGSPRKAATDGAD CCCCCCCCCCCCCCC	45.84	-
90	Phosphorylation	GSPRKAATDGADTPF CCCCCCCCCCCCCCC	39.56	22199227
95	Phosphorylation	AATDGADTPFGRSES CCCCCCCCCCCCCCC	21.71	21815630
100	Phosphorylation	ADTPFGRSESAPTLH CCCCCCCCCCCCCCC	35.96	21945579
102	Phosphorylation	TPFGRSESAPTLHPY CCCCCCCCCCCCCCC	40.57	25159151
105	Phosphorylation	GRSESAPTLHPYSPL CCCCCCCCCCCCCCC	37.10	21945579
109	Phosphorylation	SAPTLHPYSPLSPKG CCCCCCCCCCCCCCC	16.51	21945579
110	Phosphorylation	APTLHPYSPLSPKGR CCCCCCCCCCCCCCC	24.66	22167270
113	Phosphorylation	LHPYSPLSPKGRPSS CCCCCCCCCCCCCCC	28.13	25159151
117	Methylation	SPLSPKGRPSSPRTP CCCCCCCCCCCCCCC	33.77	115488459
119	Phosphorylation	LSPKGRPSSPRTPLY CCCCCCCCCCCCCCC	52.17	23927012
120	Phosphorylation	SPKGRPSSPRTPLYL CCCCCCCCCCCCCCC	22.53	25159151
122	Dimethylation	KGRPSSPRTPLYLQP CCCCCCCCCCCCCCC	51.42	-
122	Methylation	KGRPSSPRTPLYLQP CCCCCCCCCCCCCCC	51.42	54558235
123	Phosphorylation	GRPSSPRTPLYLQPD CCCCCCCCCCCCCCC	22.49	21945579
126	Phosphorylation	SSPRTPLYLQPDAYG CCCCCCCCCCCCCCC	12.42	21945579
132	Phosphorylation	LYLQPDAYGSLDRAT CCCCCCCCCCCCCCC	18.03	21945579
134	Phosphorylation	LQPDAYGSLDRATSP CCCCCCCCCCCCCCC	17.89	22167270
137	Methylation	DAYGSLDRATSPRPR CCCCCCCCCCCCCCC	45.09	58859091
139	Phosphorylation	YGSLDRATSPRPRAF CCCCCCCCCCCCCCC	39.91	30576142
140	Phosphorylation	GSLDRATSPRPRAFD CCCCCCCCCCCCCCC	20.23	30576142
142	Methylation	LDRATSPRPRAFDGA CCCCCCCCCCCCCCC	32.86	58859107
144	Methylation	RATSPRPRAFDGAGS CCCCCCCCCCCCCCC	49.52	58859131
151	Phosphorylation	RAFDGAGSSLGRAPS CCCCCCCCCCCCCCC	23.12	20873877
152	Phosphorylation	AFDGAGSSLGRAPSP CCCCCCCCCCCCCCC	33.65	21712546
155	Dimethylation	GAGSSLGRAPSPRPG CCCCCCCCCCCCCCC	48.93	-
155	Methylation	GAGSSLGRAPSPRPG CCCCCCCCCCCCCCC	48.93	54558251
158	Phosphorylation	SSLGRAPSPRPGPGP CCCCCCCCCCCCCCC	32.34	25159151
160	Methylation	LGRAPSPRPGPGPLR CCCCCCCCCCCCCCH	53.82	54558267
167	Dimethylation	RPGPGPLRQQGPPTP CCCCCCCHHCCCCCC	29.88	-
167	Methylation	RPGPGPLRQQGPPTP CCCCCCCHHCCCCCC	29.88	24129315
173	Phosphorylation	LRQQGPPTPFDFLGR CHHCCCCCCCCCCCC	39.46	28188228
180	Methylation	TPFDFLGRAGSPRGS CCCCCCCCCCCCCCC	37.61	24129315
183	Phosphorylation	DFLGRAGSPRGSPLA CCCCCCCCCCCCCCC	15.50	22167270
185	Methylation	LGRAGSPRGSPLAEG CCCCCCCCCCCCCCC	60.98	54558259
187	Phosphorylation	RAGSPRGSPLAEGPQ CCCCCCCCCCCCCCC	20.30	22167270
200	Methylation	PQAFFPERGPSPRPP CCCCCCCCCCCCCCC	63.66	58854889
203	Phosphorylation	FFPERGPSPRPPATA CCCCCCCCCCCCCCC	36.04	25159151
205	Methylation	PERGPSPRPPATAYD CCCCCCCCCCCCCCC	54.92	58859099
209	O-linked_Glycosylation	PSPRPPATAYDAPAS CCCCCCCCCCCCCHH	31.37	31492838
209	Phosphorylation	PSPRPPATAYDAPAS CCCCCCCCCCCCCHH	31.37	25394399
211	Phosphorylation	PRPPATAYDAPASAF CCCCCCCCCCCHHHH	14.32	21712546
216	Phosphorylation	TAYDAPASAFGSSLL CCCCCCHHHHCHHHH	24.07	25106551
220	Phosphorylation	APASAFGSSLLGSGG CCHHHHCHHHHCCCC	16.03	26356563
221	Phosphorylation	PASAFGSSLLGSGGS CHHHHCHHHHCCCCC	28.17	26356563
225	Phosphorylation	FGSSLLGSGGSAFAP HCHHHHCCCCCCCCC	39.69	26356563
228	Phosphorylation	SLLGSGGSAFAPPLR HHHCCCCCCCCCCCC	24.60	26356563
235	Methylation	SAFAPPLRAQDDLTL CCCCCCCCCCCCCCC	35.66	58859115
241	Phosphorylation	LRAQDDLTLRRRPPK CCCCCCCCCCCCCCC	25.80	18212344
243	Methylation	AQDDLTLRRRPPKAW CCCCCCCCCCCCCCC	27.59	58859123
253	Phosphorylation	PPKAWNESDLDVAYE CCCCCCHHHCCCCCC	40.02	28555341
259	Phosphorylation	ESDLDVAYEKKPSQT HHHCCCCCCCCCCCC	28.29	25394399
264	Phosphorylation	VAYEKKPSQTASYER CCCCCCCCCCCCEEE	49.39	30576142
266	Phosphorylation	YEKKPSQTASYERLD CCCCCCCCCCEEEEE	23.16	26356563
268	Phosphorylation	KKPSQTASYERLDVF CCCCCCCCEEEEEEE	30.80	30576142
269	Phosphorylation	KPSQTASYERLDVFA CCCCCCCEEEEEEEC	11.56	27259358
280	Phosphorylation	DVFARPASPSLQLLP EEECCCCCCCCCCCC	20.08	25159151
282	Phosphorylation	FARPASPSLQLLPWR ECCCCCCCCCCCCCC	26.67	25159151
291	Phosphorylation	QLLPWRESSLDGLGG CCCCCCHHCCCCCCC	27.85	23403867
292	Phosphorylation	LLPWRESSLDGLGGT CCCCCHHCCCCCCCC	26.23	27794612
299	Phosphorylation	SLDGLGGTGKDNLTS CCCCCCCCCCCCCCC	38.98	23403867
301	Ubiquitination	DGLGGTGKDNLTSAT CCCCCCCCCCCCCCC	43.19	-
305	Phosphorylation	GTGKDNLTSATLPRN CCCCCCCCCCCCCCC	23.67	29255136
306	Phosphorylation	TGKDNLTSATLPRNY CCCCCCCCCCCCCCC	23.56	29255136
308	Phosphorylation	KDNLTSATLPRNYKV CCCCCCCCCCCCCCC	36.74	29255136
313	Phosphorylation	SATLPRNYKVSPLAS CCCCCCCCCCCCCCC	17.66	23403867
314	Methylation	ATLPRNYKVSPLASD CCCCCCCCCCCCCCC	39.52	115975571
314	Ubiquitination	ATLPRNYKVSPLASD CCCCCCCCCCCCCCC	39.52	-
316	Phosphorylation	LPRNYKVSPLASDRR CCCCCCCCCCCCCCC	15.28	25159151
320	Phosphorylation	YKVSPLASDRRSDAG CCCCCCCCCCCCCCC	39.06	29255136
324	Phosphorylation	PLASDRRSDAGSYRR CCCCCCCCCCCCHHH	32.48	28555341
328	Phosphorylation	DRRSDAGSYRRSLGS CCCCCCCCHHHHCCC	19.72	28555341
332	Phosphorylation	DAGSYRRSLGSAGPS CCCCHHHHCCCCCCC	28.25	22167270
335	Phosphorylation	SYRRSLGSAGPSGTL CHHHHCCCCCCCCCC	35.03	22167270
339	Phosphorylation	SLGSAGPSGTLPRSW HCCCCCCCCCCCCCC	43.42	22617229
341	Phosphorylation	GSAGPSGTLPRSWQP CCCCCCCCCCCCCCC	37.62	25159151
344	Methylation	GPSGTLPRSWQPVSR CCCCCCCCCCCCCCC	54.35	82797337
345	Phosphorylation	PSGTLPRSWQPVSRI CCCCCCCCCCCCCCC	28.29	29759185
350	Phosphorylation	PRSWQPVSRIPMPPS CCCCCCCCCCCCCCC	31.04	29759185
357	Phosphorylation	SRIPMPPSSPQPRGA CCCCCCCCCCCCCCC	49.10	30266825
358	Phosphorylation	RIPMPPSSPQPRGAP CCCCCCCCCCCCCCC	33.07	30266825
362	Methylation	PPSSPQPRGAPRQRP CCCCCCCCCCCCCCC	48.97	82796411
373	Phosphorylation	RQRPIPLSMIFKLQN CCCCCCHHHHHHHHH	12.41	28555341
395	Phosphorylation	SRAMLPGSPLFTRAP CCCCCCCCCCCCCCC	19.25	22167270
399	Phosphorylation	LPGSPLFTRAPPPKL CCCCCCCCCCCCCCC	33.34	23403867
400	Methylation	PGSPLFTRAPPPKLQ CCCCCCCCCCCCCCC	37.45	54558227
489	Phosphorylation	GPVARPLSPTRLQPA CCCCCCCCCCCCCCC	27.23	24505115
491	Phosphorylation	VARPLSPTRLQPALP CCCCCCCCCCCCCCC	40.41	23312004
522	Methylation	AEIPRPLKRRGSMEQ HHCCCCHHHCCCHHH	42.61	115975579
522	Ubiquitination	AEIPRPLKRRGSMEQ HHCCCCHHHCCCHHH	42.61	-
526	Phosphorylation	RPLKRRGSMEQAPAV CCHHHCCCHHHCCCC	19.82	30266825
538	Phosphorylation	PAVALPPTHKKQYQQ CCCCCCCCCHHHHHH	44.37	20068231
541	Ubiquitination	ALPPTHKKQYQQIIS CCCCCCHHHHHHHHH	46.78	2189047
541 (in isoform 1)	Ubiquitination	-	46.78	21890473
543	Phosphorylation	PPTHKKQYQQIISRL CCCCHHHHHHHHHHH	15.97	27642862
567	Phosphorylation	GGPEPELSPITEGSE CCCCCCCCCCCCCCC	16.76	25159151
570	Phosphorylation	EPELSPITEGSEARA CCCCCCCCCCCCCCC	37.36	23927012
573	Phosphorylation	LSPITEGSEARAGPP CCCCCCCCCCCCCCC	23.11	20873877
594	Phosphorylation	PIPPPAPSQSSPPEQ CCCCCCCCCCCCCCC	44.81	21712546
596	Phosphorylation	PPPAPSQSSPPEQPQ CCCCCCCCCCCCCCC	49.50	23663014
597	Phosphorylation	PPAPSQSSPPEQPQS CCCCCCCCCCCCCCC	36.52	21712546
604	Phosphorylation	SPPEQPQSMEMRSVL CCCCCCCCHHHHHHH	24.32	22199227
616	Phosphorylation	SVLRKAGSPRKARRA HHHHHCCCHHHHHHH	26.79	26853621

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
84	S	Phosphorylation	Kinase	CDK1	P06493	PSP
113	S	Phosphorylation	Kinase	CDK1	P06493	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of IASPP_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of IASPP_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TF65_HUMAN	RELA	physical	12134007
SP1_HUMAN	SP1	physical	12134007
TF65_HUMAN	RELA	physical	10336463
P53_HUMAN	TP53	physical	20840860
EP300_HUMAN	EP300	physical	21513714
P53_HUMAN	TP53	physical	21513714
P53_HUMAN	TP53	physical	17906639
CBP_HUMAN	CREBBP	physical	25675294
EP300_HUMAN	EP300	physical	25675294
NF2L2_HUMAN	NFE2L2	physical	29033244
KEAP1_HUMAN	KEAP1	physical	29033244

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of IASPP_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-70; SER-183 AND SER-187,AND MASS SPECTROMETRY.	19690332 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102; SER-110; SER-113;SER-119; SER-120; THR-123; SER-134; SER-158; SER-183; SER-187;SER-203; SER-225; SER-316; SER-332; SER-526; SER-567 AND SER-597, ANDMASS SPECTROMETRY.	18669648 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-567, AND MASSSPECTROMETRY.	18691976 [Abstract]
"A probability-based approach for high-throughput proteinphosphorylation analysis and site localization."; Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.; Nat. Biotechnol. 24:1285-1292(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110; SER-113; SER-183AND SER-187, AND MASS SPECTROMETRY.	16964243 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-567, AND MASSSPECTROMETRY.	17081983 [Abstract]