dbPTM

EST1A_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	EST1A_HUMAN
UniProt AC	Q86US8
Protein Name	Telomerase-binding protein EST1A
Gene Name	SMG6
Organism	Homo sapiens (Human).
Sequence Length	1419
Subcellular Localization	Nucleus, nucleolus. Chromosome, telomere . Cytoplasm, cytosol. Particularly enriched in the nucleolus.
Protein Description	Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. May have a general role in telomere regulation. Promotes in vitro the ability of TERT to elongate telomeres. Overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at the fusion points) and did not perturb TRF2 telomeric localization. Binds to the single-stranded 5'-(GTGTGG)(4)GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER).; Plays a role in nonsense-mediated mRNA decay. Is thought to provide a link to the mRNA degradation machinery as it has endonuclease activity required to initiate NMD, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Degrades single-stranded RNA (ssRNA), but not ssDNA or dsRNA..
Protein Sequence	MAEGLERVRISASELRGILATLAPQAGSRENMKELKEARPRKDNRRPDLEIYKPGLSRLRNKPKIKEPPGSEEFKDEIVNDRDCSAVENGTQPVKDVCKELNNQEQNGPIDPENNRGQESFPRTAGQEDRSLKIIKRTKKPDLQIYQPGRRLQTVSKESASRVEEEEVLNQVEQLRVEEDECRGNVAKEEVANKPDRAEIEKSPGGGRVGAAKGEKGKRMGKGEGVRETHDDPARGRPGSAKRYSRSDKRRNRYRTRSTSSAGSNNSAEGAGLTDNGCRRRRQDRTKERPRLKKQVSVSSTDSLDEDRIDEPDGLGPRRSSERKRHLERNWSGRGEGEQKNSAKEYRGTLRVTFDAEAMNKESPMVRSARDDMDRGKPDKGLSSGGKGSEKQESKNPKQELRGRGRGILILPAHTTLSVNSAGSPESAPLGPRLLFGSGSKGSRSWGRGGTTRRLWDPNNPDQKPALKTQTPQLHFLDTDDEVSPTSWGDSRQAQASYYKFQNSDNPYYYPRTPGPASQYPYTGYNPLQYPVGPTNGVYPGPYYPGYPTPSGQYVCSPLPTSTMSPEEVEQHMRNLQQQELHRLLRVADNQELQLSNLLSRDRISPEGLEKMAQLRAELLQLYERCILLDIEFSDNQNVDQILWKNAFYQVIEKFRQLVKDPNVENPEQIRNRLLELLDEGSDFFDSLLQKLQVTYKFKLEDYMDGLAIRSKPLRKTVKYALISAQRCMICQGDIARYREQASDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKAEQMEKKQHEEFDLSPDQWRKGKKSTFRHVGDDTTRLEIWIHPSHPRSSQGTESGKDSEQENGLGSLSPSDLNKRFILSFLHAHGKLFTRIGMETFPAVAEKVLKEFQVLLQHSPSPIGSTRMLQLMTINMFAVHNSQLKDCFSEECRSVIQEQAAALGLAMFSLLVRRCTCLLKESAKAQLSSPEDQDDQDDIKVSSFVPDLKELLPSVKVWSDWMLGYPDTWNPPPTSLDLPSHVAVDVWSTLADFCNILTAVNQSEVPLYKDPDDDLTLLILEEDRLLSGFVPLLAAPQDPCYVEKTSDKVIAADCKRVTVLKYFLEALCGQEEPLLAFKGGKYVSVAPVPDTMGKEMGSQEGTRLEDEEEDVVIEDFEEDSEAEGSGGEDDIRELRAKKLALARKIAEQQRRQEKIQAVLEDHSQMRQMELEIRPLFLVPDTNGFIDHLASLARLLESRKYILVVPLIVINELDGLAKGQETDHRAGGYARVVQEKARKSIEFLEQRFESRDSCLRALTSRGNELESIAFRSEDITGQLGNNDDLILSCCLHYCKDKAKDFMPASKEEPIRLLREVVLLTDDRNLRVKALTRNVPVRDIPAFLTWAQVG

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
11	Phosphorylation	GLERVRISASELRGI CHHHEEECHHHHHHH	18.38	23403867
28	Phosphorylation	TLAPQAGSRENMKEL HHCCCCCCHHHHHHH	39.16	28348404
36	Ubiquitination	RENMKELKEARPRKD HHHHHHHHHHCCCCC	50.88	24816145
52	Phosphorylation	RRPDLEIYKPGLSRL CCCCCCCCCCCHHHH	11.18	21945579
53	Acetylation	RPDLEIYKPGLSRLR CCCCCCCCCCHHHHC	37.97	25953088
57	Phosphorylation	EIYKPGLSRLRNKPK CCCCCCHHHHCCCCC	34.87	21945579
60	Ubiquitination	KPGLSRLRNKPKIKE CCCHHHHCCCCCCCC	48.27	29967540
71	Phosphorylation	KIKEPPGSEEFKDEI CCCCCCCCHHHHHHH	39.46	28348404
83	Ubiquitination	DEIVNDRDCSAVENG HHHCCCCCCHHHHCC	33.32	22817900
85	Phosphorylation	IVNDRDCSAVENGTQ HCCCCCCHHHHCCCC	39.37	24719451
87	Ubiquitination	NDRDCSAVENGTQPV CCCCCHHHHCCCCCH	3.18	22817900
91	Phosphorylation	CSAVENGTQPVKDVC CHHHHCCCCCHHHHH	41.08	24719451
103	Ubiquitination	DVCKELNNQEQNGPI HHHHHHCCCCCCCCC	60.43	29967540
126	Ubiquitination	ESFPRTAGQEDRSLK CCCCCCCCCCHHHHH	30.69	24816145
138	Phosphorylation	SLKIIKRTKKPDLQI HHHHHHCCCCCCCEE	38.12	-
146	Phosphorylation	KKPDLQIYQPGRRLQ CCCCCEEECCCCCEE	8.83	21945579
154	Phosphorylation	QPGRRLQTVSKESAS CCCCCEEECCHHHHC	31.19	23312004
156	Phosphorylation	GRRLQTVSKESASRV CCCEEECCHHHHCHH	33.81	23312004
157	Acetylation	RRLQTVSKESASRVE CCEEECCHHHHCHHC	51.88	25953088
157	Ubiquitination	RRLQTVSKESASRVE CCEEECCHHHHCHHC	51.88	24816145
182	Ubiquitination	LRVEEDECRGNVAKE HHCCCCHHCCHHHHH	11.90	24816145
203	Phosphorylation	DRAEIEKSPGGGRVG CHHHHHCCCCCCCCC	18.91	23401153
213	Ubiquitination	GGRVGAAKGEKGKRM CCCCCCCCCCCCCCC	68.05	24816145
220	Ubiquitination	KGEKGKRMGKGEGVR CCCCCCCCCCCCCCC	8.08	29967540
229	Phosphorylation	KGEGVRETHDDPARG CCCCCCCCCCCCCCC	21.75	26074081
240	Phosphorylation	PARGRPGSAKRYSRS CCCCCCCCHHCCCCC	33.04	26074081
241	Ubiquitination	ARGRPGSAKRYSRSD CCCCCCCHHCCCCCH	13.27	29967540
245	Phosphorylation	PGSAKRYSRSDKRRN CCCHHCCCCCHHHHH	29.37	-
247	Phosphorylation	SAKRYSRSDKRRNRY CHHCCCCCHHHHHCC	41.36	-
256	Phosphorylation	KRRNRYRTRSTSSAG HHHHCCCCCCCCCCC	21.75	23090842
258	Phosphorylation	RNRYRTRSTSSAGSN HHCCCCCCCCCCCCC	31.84	23663014
259	Phosphorylation	NRYRTRSTSSAGSNN HCCCCCCCCCCCCCC	24.34	23663014
260	Phosphorylation	RYRTRSTSSAGSNNS CCCCCCCCCCCCCCC	21.31	23663014
261	Phosphorylation	YRTRSTSSAGSNNSA CCCCCCCCCCCCCCC	36.42	23663014
264	Phosphorylation	RSTSSAGSNNSAEGA CCCCCCCCCCCCCCC	32.33	23663014
267	Phosphorylation	SSAGSNNSAEGAGLT CCCCCCCCCCCCCCC	31.76	23663014
274	Phosphorylation	SAEGAGLTDNGCRRR CCCCCCCCCCCHHHH	26.88	23090842
287	Ubiquitination	RRRQDRTKERPRLKK HHCCCCCCCCCCHHC	53.25	29967540
297	Phosphorylation	PRLKKQVSVSSTDSL CCHHCCEECCCCCCC	17.49	28985074
299	Phosphorylation	LKKQVSVSSTDSLDE HHCCEECCCCCCCCC	21.73	30177828
300	Phosphorylation	KKQVSVSSTDSLDED HCCEECCCCCCCCCC	33.79	30177828
301	Phosphorylation	KQVSVSSTDSLDEDR CCEECCCCCCCCCCC	23.31	30177828
303	Phosphorylation	VSVSSTDSLDEDRID EECCCCCCCCCCCCC	37.50	30177828
332	Phosphorylation	RHLERNWSGRGEGEQ HHHHHHCCCCCCCCC	23.35	23401153
353	Phosphorylation	YRGTLRVTFDAEAMN HEEEEEEEECHHHHC	14.90	24905233
363	Phosphorylation	AEAMNKESPMVRSAR HHHHCCCCCCHHHCH	21.65	25159151
367	Ubiquitination	NKESPMVRSARDDMD CCCCCCHHHCHHHCC	20.53	24816145
368	Phosphorylation	KESPMVRSARDDMDR CCCCCHHHCHHHCCC	19.08	24905233
398	Ubiquitination	KQESKNPKQELRGRG HCCCCCCCHHHCCCC	66.84	24816145
401	Ubiquitination	SKNPKQELRGRGRGI CCCCCHHHCCCCCEE	6.97	29967540
404	Dimethylation	PKQELRGRGRGILIL CCHHHCCCCCEEEEE	25.79	-
404	Methylation	PKQELRGRGRGILIL CCHHHCCCCCEEEEE	25.79	-
406	Dimethylation	QELRGRGRGILILPA HHHCCCCCEEEEECC	27.84	-
406	Methylation	QELRGRGRGILILPA HHHCCCCCEEEEECC	27.84	-
424	Phosphorylation	LSVNSAGSPESAPLG EEECCCCCCCCCCCC	25.80	-
433	Methylation	ESAPLGPRLLFGSGS CCCCCCCEEEECCCC	42.07	-
438	Phosphorylation	GPRLLFGSGSKGSRS CCEEEECCCCCCCCC	32.34	23911959
440	Phosphorylation	RLLFGSGSKGSRSWG EEEECCCCCCCCCCC	35.22	22199227
441	Ubiquitination	LLFGSGSKGSRSWGR EEECCCCCCCCCCCC	65.51	-
443	Phosphorylation	FGSGSKGSRSWGRGG ECCCCCCCCCCCCCC	27.20	23186163
468	Ubiquitination	PDQKPALKTQTPQLH CCCCCCCCCCCCCEE	40.47	29967540
469	Phosphorylation	DQKPALKTQTPQLHF CCCCCCCCCCCCEEE	38.23	23927012
471	Phosphorylation	KPALKTQTPQLHFLD CCCCCCCCCCEEECC	20.45	23927012
479	Phosphorylation	PQLHFLDTDDEVSPT CCEEECCCCCCCCCC	47.71	23927012
484	Phosphorylation	LDTDDEVSPTSWGDS CCCCCCCCCCCCCCC	22.03	30266825
486	Phosphorylation	TDDEVSPTSWGDSRQ CCCCCCCCCCCCCCH	29.61	30266825
487	Phosphorylation	DDEVSPTSWGDSRQA CCCCCCCCCCCCCHH	32.23	30266825
491	Phosphorylation	SPTSWGDSRQAQASY CCCCCCCCCHHHHHE	23.50	30266825
508	Phosphorylation	FQNSDNPYYYPRTPG ECCCCCCCCCCCCCC	23.14	28796482
509	Phosphorylation	QNSDNPYYYPRTPGP CCCCCCCCCCCCCCC	14.30	28796482
510	Phosphorylation	NSDNPYYYPRTPGPA CCCCCCCCCCCCCCH	4.76	28796482
512	Methylation	DNPYYYPRTPGPASQ CCCCCCCCCCCCHHH	37.11	-
629	Ubiquitination	LYERCILLDIEFSDN HHHHHHHHCCCCCCC	2.94	24816145
660	Ubiquitination	EKFRQLVKDPNVENP HHHHHHHCCCCCCCH	75.86	24816145
711	Phosphorylation	MDGLAIRSKPLRKTV CCCEEECCCCHHHHH	32.29	24719451
769	Phosphorylation	APKNGRPYNQLALLA CCCCCCCCHHHHHHH	17.74	26657352
778	Phosphorylation	QLALLAVYTRRKLDA HHHHHHHHHHHHHHH	6.74	30206219
779	Phosphorylation	LALLAVYTRRKLDAV HHHHHHHHHHHHHHH	19.87	30206219
788	Phosphorylation	RKLDAVYYYMRSLAA HHHHHHHHHHHHHHH	5.56	-
789	Phosphorylation	KLDAVYYYMRSLAAS HHHHHHHHHHHHHHC	2.88	-
796	Phosphorylation	YMRSLAASNPILTAK HHHHHHHCCCCCCHH	37.22	-
803	Acetylation	SNPILTAKESLMSLF CCCCCCHHHHHHHHH	42.47	7667043
814	Ubiquitination	MSLFEETKRKAEQME HHHHHHHHHHHHHHH	56.00	-
823	Ubiquitination	KAEQMEKKQHEEFDL HHHHHHHHHHHCCCC	43.51	29967540
831	Phosphorylation	QHEEFDLSPDQWRKG HHHCCCCCHHHHHCC	28.42	25159151
860	Phosphorylation	LEIWIHPSHPRSSQG EEEEECCCCCCCCCC	31.77	23898821
864	Phosphorylation	IHPSHPRSSQGTESG ECCCCCCCCCCCCCC	31.31	23927012
865	Phosphorylation	HPSHPRSSQGTESGK CCCCCCCCCCCCCCC	33.63	23927012
868	Phosphorylation	HPRSSQGTESGKDSE CCCCCCCCCCCCCCC	21.24	23927012
870	Phosphorylation	RSSQGTESGKDSEQE CCCCCCCCCCCCCCC	51.48	23401153
874	Phosphorylation	GTESGKDSEQENGLG CCCCCCCCCCCCCCC	44.67	23927012
882	Phosphorylation	EQENGLGSLSPSDLN CCCCCCCCCCHHHHH	30.62	23403867
884	Phosphorylation	ENGLGSLSPSDLNKR CCCCCCCCHHHHHHH	24.85	25159151
886	Phosphorylation	GLGSLSPSDLNKRFI CCCCCCHHHHHHHHH	51.89	23403867
918	Acetylation	TFPAVAEKVLKEFQV CCHHHHHHHHHHHHH	43.03	15612443
960	Ubiquitination	SQLKDCFSEECRSVI HHHHHHCCHHHHHHH	38.52	22817900
964	Ubiquitination	DCFSEECRSVIQEQA HHCCHHHHHHHHHHH	36.92	22817900
987	Phosphorylation	SLLVRRCTCLLKESA HHHHHHHHHHHHHHH	12.36	23403867
991	Ubiquitination	RRCTCLLKESAKAQL HHHHHHHHHHHHHHC	36.35	22817900
995	Ubiquitination	CLLKESAKAQLSSPE HHHHHHHHHHCCCCC	46.04	22817900
995 (in isoform 1)	Ubiquitination	-	46.04	21906983
999	Phosphorylation	ESAKAQLSSPEDQDD HHHHHHCCCCCCCCC	31.59	25159151
1000	Phosphorylation	SAKAQLSSPEDQDDQ HHHHHCCCCCCCCCH	40.68	21815630
1011	Ubiquitination	QDDQDDIKVSSFVPD CCCHHCCCHHHCCCC	42.96	29967540
1025	Phosphorylation	DLKELLPSVKVWSDW CHHHHHHCCCEECCH	34.24	27794612
1149	Ubiquitination	EEPLLAFKGGKYVSV CCCEEEEECCCEEEE	62.23	29967540
1152	Ubiquitination	LLAFKGGKYVSVAPV EEEEECCCEEEEEEC	51.66	-
1169	Phosphorylation	TMGKEMGSQEGTRLE CCCHHCCCCCCCCCC	24.61	28634120
1173	Phosphorylation	EMGSQEGTRLEDEEE HCCCCCCCCCCCCCC	31.18	28634120
1191	Phosphorylation	IEDFEEDSEAEGSGG EECCCCCCCCCCCCC	41.82	27422710
1196	Phosphorylation	EDSEAEGSGGEDDIR CCCCCCCCCCHHHHH	34.82	27422710
1225	Ubiquitination	EQQRRQEKIQAVLED HHHHHHHHHHHHHHC	31.60	-
1236	Sulfoxidation	VLEDHSQMRQMELEI HHHCHHHHHHCCEEE	3.44	21406390
1309	Ubiquitination	VVQEKARKSIEFLEQ HHHHHHHHHHHHHHH	61.72	29967540
1346	Phosphorylation	AFRSEDITGQLGNND EEECCCCCCCCCCCH	31.06	29759185
1358	Phosphorylation	NNDDLILSCCLHYCK CCHHHHHHHHHHHHH	8.87	29759185
1376	Ubiquitination	KDFMPASKEEPIRLL HHCCCCCCHHHHHHH	69.10	29967540
1390	Phosphorylation	LREVVLLTDDRNLRV HHEEEEECCCCCHHH	31.52	20860994

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of EST1A_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of EST1A_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
256	Phosphorylation	263 (7)	K ⇒ Q	rs216195	Intraocular pressure	29617998
258	Phosphorylation	263 (5)	K ⇒ Q	rs216195	Intraocular pressure	29617998
259	Phosphorylation	263 (4)	K ⇒ Q	rs216195	Intraocular pressure	29617998
260	Phosphorylation	263 (3)	K ⇒ Q	rs216195	Intraocular pressure	29617998
261	Phosphorylation	263 (2)	K ⇒ Q	rs216195	Intraocular pressure	29617998
267	Phosphorylation	263 (4)	K ⇒ Q	rs216195	Intraocular pressure	29617998

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
HDAC8_HUMAN	HDAC8	physical	16809764
HBB_HUMAN	HBB	physical	21145460
RBM8A_HUMAN	RBM8A	physical	20930030
RENT1_HUMAN	UPF1	physical	20930030
MGN_HUMAN	MAGOH	physical	20930030
PABP1_HUMAN	PABPC1	physical	20930030
EST1A_HUMAN	SMG6	physical	20930030
REN3B_HUMAN	UPF3B	physical	20930030
SMG5_HUMAN	SMG5	physical	20930030
SMG7_HUMAN	SMG7	physical	20930030

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of EST1A_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, AND MASSSPECTROMETRY.	19413330 [Abstract]
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-479, AND MASSSPECTROMETRY.	19690332 [Abstract]