C1TC_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: C1TC_HUMAN
• UniProt AC: P11586
• Protein Name: C-1-tetrahydrofolate synthase, cytoplasmic
• Gene Name: MTHFD1
• Organism: Homo sapiens (Human).
• Sequence Length: 935
• Subcellular Localization: Cytoplasm.
• Protein Sequence: MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTAHLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGETKAKVLLSALERLKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLYQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFSDIQIRRLKRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MAPAEILN -------CCCHHHHC	10.20	19413330
1	Sulfoxidation	-------MAPAEILN -------CCCHHHHC	10.20	28183972
10	Acetylation	PAEILNGKEISAQIR CHHHHCCCHHHHHHH	51.29	22424773
10	Malonylation	PAEILNGKEISAQIR CHHHHCCCHHHHHHH	51.29	26320211
10	Ubiquitination	PAEILNGKEISAQIR CHHHHCCCHHHHHHH	51.29	-
21	Malonylation	AQIRARLKNQVTQLK HHHHHHHHHHHHHHH	39.52	26320211
21	Ubiquitination	AQIRARLKNQVTQLK HHHHHHHHHHHHHHH	39.52	-
25	Phosphorylation	ARLKNQVTQLKEQVP HHHHHHHHHHHHHCC	20.58	20068231
28	Ubiquitination	KNQVTQLKEQVPGFT HHHHHHHHHHCCCCC	35.96	21906983
35	Phosphorylation	KEQVPGFTPRLAILQ HHHCCCCCCCEEEEE	17.26	23312004
37	Methylation	QVPGFTPRLAILQVG HCCCCCCCEEEEEEC	33.42	115483999
52	Nitration	NRDDSNLYINVKLKA CCCCCCEEEEEEEEE	8.49	-
52	Phosphorylation	NRDDSNLYINVKLKA CCCCCCEEEEEEEEE	8.49	-
56	Acetylation	SNLYINVKLKAAEEI CCEEEEEEEEEHHHH	39.52	23954790
58	Acetylation	LYINVKLKAAEEIGI EEEEEEEEEHHHHCC	39.57	27178108
58	Malonylation	LYINVKLKAAEEIGI EEEEEEEEEHHHHCC	39.57	26320211
58	Ubiquitination	LYINVKLKAAEEIGI EEEEEEEEEHHHHCC	39.57	-
66	2-Hydroxyisobutyrylation	AAEEIGIKATHIKLP EHHHHCCCEEEEECC	41.86	-
66	Acetylation	AAEEIGIKATHIKLP EHHHHCCCEEEEECC	41.86	25953088
66	Malonylation	AAEEIGIKATHIKLP EHHHHCCCEEEEECC	41.86	26320211
66	Ubiquitination	AAEEIGIKATHIKLP EHHHHCCCEEEEECC	41.86	-
71	Acetylation	GIKATHIKLPRTTTE CCCEEEEECCCCCCH	44.85	25953088
71	Ubiquitination	GIKATHIKLPRTTTE CCCEEEEECCCCCCH	44.85	-
75	Phosphorylation	THIKLPRTTTESEVM EEEECCCCCCHHHHH	35.81	26437602
79	Phosphorylation	LPRTTTESEVMKYIT CCCCCCHHHHHHHHH	33.14	26437602
82	Sulfoxidation	TTTESEVMKYITSLN CCCHHHHHHHHHHCC	2.09	30846556
108	Phosphorylation	LPLDSENSINTEEVI CCCCCCCCCCHHHHH	17.11	22468782
128	Phosphorylation	EKDVDGLTSINAGRL CCCCCCCCCCCCCCC	32.84	20873877
129	Phosphorylation	KDVDGLTSINAGRLA CCCCCCCCCCCCCCC	20.91	20873877
148	Phosphorylation	NDCFIPCTPKGCLEL CCCCCCCCCHHHHHH	23.76	25159151
150	Ubiquitination	CFIPCTPKGCLELIK CCCCCCCHHHHHHHH	44.07	-
152	Glutathionylation	IPCTPKGCLELIKET CCCCCHHHHHHHHHH	3.13	22555962
157	Acetylation	KGCLELIKETGVPIA HHHHHHHHHHCCCCC	62.94	25953088
157	Ubiquitination	KGCLELIKETGVPIA HHHHHHHHHHCCCCC	62.94	-
191	Phosphorylation	LLLWNNATVTTCHSK EEEECCCEEEECCCC	22.10	28857561
193	Phosphorylation	LWNNATVTTCHSKTA EECCCEEEECCCCCC	21.25	28348404
194	Phosphorylation	WNNATVTTCHSKTAH ECCCEEEECCCCCCC	12.13	28348404
197	Phosphorylation	ATVTTCHSKTAHLDE CEEEECCCCCCCCCC	33.57	20873877
198	Ubiquitination	TVTTCHSKTAHLDEE EEEECCCCCCCCCCC	26.51	-
223	Sumoylation	TGQPEMVKGEWIKPG CCCCCEECCCEECCC	50.21	-
223	Ubiquitination	TGQPEMVKGEWIKPG CCCCCEECCCEECCC	50.21	-
228	Ubiquitination	MVKGEWIKPGAIVID EECCCEECCCEEEEE	38.17	-
236	Glutathionylation	PGAIVIDCGINYVPD CCEEEEECCCCCCCC	3.97	22555962
251	Malonylation	DKKPNGRKVVGDVAY CCCCCCCEEECCEEH	42.54	26320211
251	Ubiquitination	DKKPNGRKVVGDVAY CCCCCCCEEECCEEH	42.54	-
258	Nitration	KVVGDVAYDEAKERA EEECCEEHHHHHHHH	17.73	-
258	Phosphorylation	KVVGDVAYDEAKERA EEECCEEHHHHHHHH	17.73	28152594
262	2-Hydroxyisobutyrylation	DVAYDEAKERASFIT CEEHHHHHHHHCEEE	45.76	-
262	Acetylation	DVAYDEAKERASFIT CEEHHHHHHHHCEEE	45.76	23954790
262	Ubiquitination	DVAYDEAKERASFIT CEEHHHHHHHHCEEE	45.76	21906983
266	Phosphorylation	DEAKERASFITPVPG HHHHHHHCEEECCCC	24.33	21406692
269	Phosphorylation	KERASFITPVPGGVG HHHHCEEECCCCCCC	19.07	21406692
279	Phosphorylation	PGGVGPMTVAMLMQS CCCCCHHHHHHHHHH	14.30	21406692
286	Phosphorylation	TVAMLMQSTVESAKR HHHHHHHHHHHHHHH	22.06	22210691
287	Phosphorylation	VAMLMQSTVESAKRF HHHHHHHHHHHHHHH	16.05	22210691
290	Phosphorylation	LMQSTVESAKRFLEK HHHHHHHHHHHHHHH	34.45	24719451
297	2-Hydroxyisobutyrylation	SAKRFLEKFKPGKWM HHHHHHHHHCCCCEE	61.92	-
297	Acetylation	SAKRFLEKFKPGKWM HHHHHHHHHCCCCEE	61.92	26051181
302	Acetylation	LEKFKPGKWMIQYNN HHHHCCCCEEEEECC	41.96	25953088
304	Sulfoxidation	KFKPGKWMIQYNNLN HHCCCCEEEEECCCC	1.24	28183972
307	Phosphorylation	PGKWMIQYNNLNLKT CCCEEEEECCCCCCC	8.62	29496907
313	Ubiquitination	QYNNLNLKTPVPSDI EECCCCCCCCCCCCC	50.11	21906983
314	Phosphorylation	YNNLNLKTPVPSDID ECCCCCCCCCCCCCC	32.46	21815630
318	Phosphorylation	NLKTPVPSDIDISRS CCCCCCCCCCCCCCC	47.64	21815630
323	Phosphorylation	VPSDIDISRSCKPKP CCCCCCCCCCCCCCC	17.88	21815630
324	Methylation	PSDIDISRSCKPKPI CCCCCCCCCCCCCCH	46.54	115484023
333	2-Hydroxyisobutyrylation	CKPKPIGKLAREIGL CCCCCHHHHHHHHCC	39.43	-
333	Acetylation	CKPKPIGKLAREIGL CCCCCHHHHHHHHCC	39.43	25953088
333	Ubiquitination	CKPKPIGKLAREIGL CCCCCHHHHHHHHCC	39.43	-
352	Ubiquitination	VELYGETKAKVLLSA EHHHCHHHHHHHHHH	41.74	-
354	Ubiquitination	LYGETKAKVLLSALE HHCHHHHHHHHHHHH	34.66	21906983
354	Ubiquitination	LYGETKAKVLLSALE HHCHHHHHHHHHHHH	34.66	21906983
358	Phosphorylation	TKAKVLLSALERLKH HHHHHHHHHHHHHCC	27.01	21712546
364	Ubiquitination	LSALERLKHRPDGKY HHHHHHHCCCCCCCE	43.73	-
370	Ubiquitination	LKHRPDGKYVVVTGI HCCCCCCCEEEEEEE	42.10	21906983
370	2-Hydroxyisobutyrylation	LKHRPDGKYVVVTGI HCCCCCCCEEEEEEE	42.10	-
370	Ubiquitination	LKHRPDGKYVVVTGI HCCCCCCCEEEEEEE	42.10	21906983
371	Phosphorylation	KHRPDGKYVVVTGIT CCCCCCCEEEEEEEC	12.19	28152594
375	Phosphorylation	DGKYVVVTGITPTPL CCCEEEEEEECCCCC	15.97	28152594
380	Phosphorylation	VVTGITPTPLGEGKS EEEEECCCCCCCCCC	23.61	20071362
402	Phosphorylation	QALGAHLYQNVFACV HHHHHHHHHCEEECC	6.54	22461510
408	S-palmitoylation	LYQNVFACVRQPSQG HHHCEEECCCCCCCC	1.44	29575903
410	Ubiquitination	QNVFACVRQPSQGPT HCEEECCCCCCCCCC	42.44	21890473
413	Phosphorylation	FACVRQPSQGPTFGI EECCCCCCCCCCCEE	39.16	20873877
426	Ubiquitination	GIKGGAAGGGYSQVI EECCCCCCCCCCCEE	29.53	21890473
469	Phosphorylation	ARIFHELTQTDKALF HHHHHHHHHHCHHHH	26.83	20068231
471	Phosphorylation	IFHELTQTDKALFNR HHHHHHHHCHHHHHH	33.94	20068231
473	Ubiquitination	HELTQTDKALFNRLV HHHHHHCHHHHHHHC	50.83	21906983
473	2-Hydroxyisobutyrylation	HELTQTDKALFNRLV HHHHHHCHHHHHHHC	50.83	-
473	Acetylation	HELTQTDKALFNRLV HHHHHHCHHHHHHHC	50.83	23954790
473	Ubiquitination	HELTQTDKALFNRLV HHHHHHCHHHHHHHC	50.83	21906983
482	Phosphorylation	LFNRLVPSVNGVRRF HHHHHCCCCCCCCCC	22.27	20873877
488	Methylation	PSVNGVRRFSDIQIR CCCCCCCCCCHHHHH	32.77	72609329
490	Phosphorylation	VNGVRRFSDIQIRRL CCCCCCCCHHHHHHH	31.80	30266825
495	Methylation	RFSDIQIRRLKRLGI CCCHHHHHHHHHCCC	22.85	115484007
504	Ubiquitination	LKRLGIEKTDPTTLT HHHCCCCCCCCCCCC	57.20	21906983
505	Phosphorylation	KRLGIEKTDPTTLTD HHCCCCCCCCCCCCH	34.13	-
511	Phosphorylation	KTDPTTLTDEEINRF CCCCCCCCHHHHHHH	38.99	-
527	Phosphorylation	RLDIDPETITWQRVL CCCCCHHHCCEEEEE	29.15	21406692
529	Ubiquitination	DIDPETITWQRVLDT CCCHHHCCEEEEECC	23.98	21890473
529	Phosphorylation	DIDPETITWQRVLDT CCCHHHCCEEEEECC	23.98	21406692
536	Phosphorylation	TWQRVLDTNDRFLRK CEEEEECCCCCHHHH	34.55	-
539	Methylation	RVLDTNDRFLRKITI EEECCCCCHHHHCEE	34.27	-
543	2-Hydroxyisobutyrylation	TNDRFLRKITIGQAP CCCCHHHHCEECCCC	46.47	-
543	Malonylation	TNDRFLRKITIGQAP CCCCHHHHCEECCCC	46.47	26320211
543	Ubiquitination	TNDRFLRKITIGQAP CCCCHHHHCEECCCC	46.47	-
545	Phosphorylation	DRFLRKITIGQAPTE CCHHHHCEECCCCCC	23.30	20873877
551	Phosphorylation	ITIGQAPTEKGHTRT CEECCCCCCCCCCCE	54.03	20873877
553	Acetylation	IGQAPTEKGHTRTAQ ECCCCCCCCCCCEEE	59.47	23954790
553	Malonylation	IGQAPTEKGHTRTAQ ECCCCCCCCCCCEEE	59.47	26320211
553	Ubiquitination	IGQAPTEKGHTRTAQ ECCCCCCCCCCCEEE	59.47	19608861
558	Phosphorylation	TEKGHTRTAQFDISV CCCCCCCEEECCHHH	26.43	22210691
567	Phosphorylation	QFDISVASEIMAVLA ECCHHHHHHHHHHHH	26.04	22210691
577	Phosphorylation	MAVLALTTSLEDMRE HHHHHHHCCHHHHHH	31.97	22210691
588	Ubiquitination	DMRERLGKMVVASSK HHHHHHHCHHHHCCC	32.87	21906983
588	Acetylation	DMRERLGKMVVASSK HHHHHHHCHHHHCCC	32.87	25953088
588	Ubiquitination	DMRERLGKMVVASSK HHHHHHHCHHHHCCC	32.87	21906983
589	Sulfoxidation	MRERLGKMVVASSKK HHHHHHCHHHHCCCC	2.52	30846556
595	Acetylation	KMVVASSKKGEPVSA CHHHHCCCCCCCCCH	63.40	25953088
601	Phosphorylation	SKKGEPVSAEDLGVS CCCCCCCCHHHHCCC	36.33	28857561
608	Phosphorylation	SAEDLGVSGALTVLM CHHHHCCCHHHHHHC	19.29	-
612	Phosphorylation	LGVSGALTVLMKDAI HCCCHHHHHHCHHCC	15.85	-
644	Ubiquitination	GPFANIAHGNSSIIA ECCCCCCCCCCCHHH	31.97	21890473
684	Acetylation	MEKFFNIKCRYSGLC HHHHCCCEECCCCCC	17.48	25953088
684	Succinylation	MEKFFNIKCRYSGLC HHHHCCCEECCCCCC	17.48	23954790
684	Ubiquitination	MEKFFNIKCRYSGLC HHHHCCCEECCCCCC	17.48	-
721	Acetylation	TAGLPLPKAYIQENL CCCCCCCHHHHHHHH	62.60	23954790
721	Ubiquitination	TAGLPLPKAYIQENL CCCCCCCHHHHHHHH	62.60	-
723	Phosphorylation	GLPLPKAYIQENLEL CCCCCHHHHHHHHHH	14.84	28152594
733	Ubiquitination	ENLELVEKGFSNLKK HHHHHHHHHHHHHHH	58.75	-
739	2-Hydroxyisobutyrylation	EKGFSNLKKQIENAR HHHHHHHHHHHHHHH	47.05	-
739	Acetylation	EKGFSNLKKQIENAR HHHHHHHHHHHHHHH	47.05	25953088
739	Ubiquitination	EKGFSNLKKQIENAR HHHHHHHHHHHHHHH	47.05	-
747	Sulfoxidation	KQIENARMFGIPVVV HHHHHHHHHCCCEEE	3.16	28183972
761	Phosphorylation	VAVNAFKTDTESELD EEEECCCCCCHHHHH	41.52	-
763	Phosphorylation	VNAFKTDTESELDLI EECCCCCCHHHHHHH	46.79	27732954
765	Phosphorylation	AFKTDTESELDLISR CCCCCCHHHHHHHHH	45.62	27732954
771	Phosphorylation	ESELDLISRLSREHG HHHHHHHHHHHHHHC	34.17	21712546
784	Ubiquitination	HGAFDAVKCTHWAEG HCCCCEEECCCCCCC	34.83	21906983
784	Ubiquitination	HGAFDAVKCTHWAEG HCCCCEEECCCCCCC	34.83	21906983
786	Phosphorylation	AFDAVKCTHWAEGGK CCCEEECCCCCCCCH	18.09	21082442
793	Acetylation	THWAEGGKGALALAQ CCCCCCCHHHHHHHH	52.44	26051181
793	Ubiquitination	THWAEGGKGALALAQ CCCCCCCHHHHHHHH	52.44	-
810	Phosphorylation	QRAAQAPSSFQLLYD HHHHCCCCHHHHHHC	46.96	28857561
811	Phosphorylation	RAAQAPSSFQLLYDL HHHCCCCHHHHHHCC	18.89	27050516
819	Acetylation	FQLLYDLKLPVEDKI HHHHHCCCCCHHHHH	48.09	23954790
819	Succinylation	FQLLYDLKLPVEDKI HHHHHCCCCCHHHHH	48.09	23954790
819	Ubiquitination	FQLLYDLKLPVEDKI HHHHHCCCCCHHHHH	48.09	-
825	2-Hydroxyisobutyrylation	LKLPVEDKIRIIAQK CCCCHHHHHHHHHHH	22.00	-
825	Acetylation	LKLPVEDKIRIIAQK CCCCHHHHHHHHHHH	22.00	26051181
825	Ubiquitination	LKLPVEDKIRIIAQK CCCCHHHHHHHHHHH	22.00	-
832	Acetylation	KIRIIAQKIYGADDI HHHHHHHHHHCCCCH	28.95	25953088
832	Ubiquitination	KIRIIAQKIYGADDI HHHHHHHHHHCCCCH	28.95	-
834	Phosphorylation	RIIAQKIYGADDIEL HHHHHHHHCCCCHHC	17.19	28152594
840	Ubiquitination	IYGADDIELLPEAQH HHCCCCHHCCCHHHH	51.77	21890473
848	Ubiquitination	LLPEAQHKAEVYTKQ CCCHHHHHCEEECCC	33.88	21906983
854	Acetylation	HKAEVYTKQGFGNLP HHCEEECCCCCCCCC	30.64	25953088
854	Ubiquitination	HKAEVYTKQGFGNLP HHCEEECCCCCCCCC	30.64	-
863	Glutathionylation	GFGNLPICMAKTHLS CCCCCCEEEEEECCC	1.73	22555962
866	Acetylation	NLPICMAKTHLSLSH CCCEEEEEECCCCCC	15.03	25953088
866	Ubiquitination	NLPICMAKTHLSLSH CCCEEEEEECCCCCC	15.03	-
867	Phosphorylation	LPICMAKTHLSLSHN CCEEEEEECCCCCCC	20.64	26074081
870	Phosphorylation	CMAKTHLSLSHNPEQ EEEEECCCCCCCHHH	21.88	23401153
872	Phosphorylation	AKTHLSLSHNPEQKG EEECCCCCCCHHHCC	20.17	26074081
882	Phosphorylation	PEQKGVPTGFILPIR HHHCCCCCEEEEEHH	42.17	21712546

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of C1TC_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of C1TC_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of C1TC_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SYIM_HUMAN	IARS2	physical	22939629
P5CS_HUMAN	ALDH18A1	physical	22939629
SYSM_HUMAN	SARS2	physical	22939629
CAVN3_HUMAN	PRKCDBP	physical	22939629
PPT2_HUMAN	PPT2	physical	22939629
ICT1_HUMAN	ICT1	physical	22939629
PYC_HUMAN	PC	physical	22939629
RU17_HUMAN	SNRNP70	physical	22939629
CADH2_HUMAN	CDH2	physical	22939629
RM49_HUMAN	MRPL49	physical	22939629
PERI_HUMAN	PRPH	physical	22939629
TM165_HUMAN	TMEM165	physical	22939629
FAF2_HUMAN	FAF2	physical	22939629
RM23_HUMAN	MRPL23	physical	22939629
SFXN1_HUMAN	SFXN1	physical	22939629
RL32_HUMAN	RPL32	physical	22939629
LYAG_HUMAN	GAA	physical	22939629
DREB_HUMAN	DBN1	physical	22939629
SF3B3_HUMAN	SF3B3	physical	22939629
METK2_HUMAN	MAT2A	physical	22863883
PAPOA_HUMAN	PAPOLA	physical	26344197
PFKAL_HUMAN	PFKL	physical	26344197
PRP19_HUMAN	PRPF19	physical	26344197
RL35A_HUMAN	RPL35A	physical	26344197
RL7_HUMAN	RPL7	physical	26344197
SAR1A_HUMAN	SAR1A	physical	26344197
UBA1_HUMAN	UBA1	physical	26344197
XPP1_HUMAN	XPNPEP1	physical	26344197

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 601634: Neural tube defects, folate-sensitive (NTDFS)
• 114500: Colorectal cancer (CRC)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• DB00116: Tetrahydrofolic acid

Related Literatures of Post-Translational Modification

Acetylation

"Lysine acetylation targets protein complexes and co-regulates majorcellular functions.";
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.;
Science 325:834-840(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-553, AND MASS SPECTROMETRY.
PubMed: 19608861