SYIM_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SYIM_HUMAN
• UniProt AC: Q9NSE4
• Protein Name: Isoleucine--tRNA ligase, mitochondrial
• Gene Name: IARS2
• Organism: Homo sapiens (Human).
• Sequence Length: 1012
• Subcellular Localization: Mitochondrion matrix.
• Protein Sequence: MRWGLRPRGPGAAALATARSLWGTPRLPCSPGWQGATKRLLVRSVSGASNHQPNSNSGRYRDTVLLPQTSFPMKLLGRQQPDTELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRFHMMNGSKIHFVPGWDCHGLPIEIKVLSELGREAQNLSAMEIRKKARSFAKAAIEKQKSAFIRWGIMADWNNCYYTFDGKYEAKQLRTFYQMYDKGLVYRSYKPVFWSPSSRTALAEAELEYNPEHVSRSIYVKFPLLKPSPKLASLIDGSSPVSILVWTTQPWTIPANEAVCYMPESKYAVVKCSKSGDLYVLAADKVASVASTLETTFETISTLSGVDLENGTCSHPLIPDKASPLLPANHVTMAKGTGLVHTAPAHGMEDYGVASQHNLPMDCLVDEDGVFTDVAGPELQNKAVLEEGTDVVIKMLQTAKNLLKEEKLVHSYPYDWRTKKPVVIRASKQWFINITDIKTAAKELLKKVKFIPGSALNGMVEMMDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIVKLVEQHGSDIWWTLPPEQLLPKEVLSEVGGPDALEYVPGQDILDIWFDSGTSWSYVLPGPDQRADLYLEGKDQLGGWFQSSLLTSVAARKRAPYKTVIVHGFTLGEKGEKMSKSLGNVIHPDVVVNGGQDQSKEPPYGADVLRWWVADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADFNPETDSIPVNDMYVIDQYMLHLLQDLANKITELYKQYDFGKVVRLLRTFYTRELSNFYFSIIKDRLYCEKENDPKRRSCQTALVEILDVIVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVITVIEPGLLFEIIEMLQSEETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREESSYKVIVMPTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
44	Phosphorylation	TKRLLVRSVSGASNH HHHHEEEECCCCCCC	17.10	-
46	Phosphorylation	RLLVRSVSGASNHQP HHEEEECCCCCCCCC	29.86	23898821
57	Phosphorylation	NHQPNSNSGRYRDTV CCCCCCCCCCCCCEE	25.19	-
60	Phosphorylation	PNSNSGRYRDTVLLP CCCCCCCCCCEEEEE	19.16	22817900
70	Phosphorylation	TVLLPQTSFPMKLLG EEEEECCCCCHHHCC	23.31	30631047
74	Acetylation	PQTSFPMKLLGRQQP ECCCCCHHHCCCCCC	40.58	25953088
74	Malonylation	PQTSFPMKLLGRQQP ECCCCCHHHCCCCCC	40.58	26320211
74	Succinylation	PQTSFPMKLLGRQQP ECCCCCHHHCCCCCC	40.58	-
74	Succinylation	PQTSFPMKLLGRQQP ECCCCCHHHCCCCCC	40.58	23954790
83	Phosphorylation	LGRQQPDTELEIQQK CCCCCCCCCHHHHHH	49.50	22468782
94	Phosphorylation	IQQKCGFSELYSWQR HHHHHCHHHHHHHHH	16.39	22468782
97	Phosphorylation	KCGFSELYSWQRERK HHCHHHHHHHHHHHC	12.10	22468782
133	Ubiquitination	HALNKILKDIANRFH HHHHHHHHHHHHHHH	51.03	29967540
163	Acetylation	HGLPIEIKVLSELGR CCCCHHHHHHHHHHH	25.08	25038526
166	Phosphorylation	PIEIKVLSELGREAQ CHHHHHHHHHHHHHH	33.49	28060719
176	Phosphorylation	GREAQNLSAMEIRKK HHHHHHCCHHHHHHH	32.87	29396449
178	Sulfoxidation	EAQNLSAMEIRKKAR HHHHCCHHHHHHHHH	3.85	21406390
186	Phosphorylation	EIRKKARSFAKAAIE HHHHHHHHHHHHHHH	34.12	20068231
189	2-Hydroxyisobutyrylation	KKARSFAKAAIEKQK HHHHHHHHHHHHHHH	36.02	-
189	Acetylation	KKARSFAKAAIEKQK HHHHHHHHHHHHHHH	36.02	19608861
189	Malonylation	KKARSFAKAAIEKQK HHHHHHHHHHHHHHH	36.02	26320211
189	Ubiquitination	KKARSFAKAAIEKQK HHHHHHHHHHHHHHH	36.02	19608861
194	2-Hydroxyisobutyrylation	FAKAAIEKQKSAFIR HHHHHHHHHHHHHHH	57.84	-
194	Succinylation	FAKAAIEKQKSAFIR HHHHHHHHHHHHHHH	57.84	-
194	Succinylation	FAKAAIEKQKSAFIR HHHHHHHHHHHHHHH	57.84	-
222	2-Hydroxyisobutyrylation	FDGKYEAKQLRTFYQ ECCCEEHHHHHHHHH	37.61	-
222	Acetylation	FDGKYEAKQLRTFYQ ECCCEEHHHHHHHHH	37.61	22424773
222	Malonylation	FDGKYEAKQLRTFYQ ECCCEEHHHHHHHHH	37.61	26320211
225	Methylation	KYEAKQLRTFYQMYD CEEHHHHHHHHHHHH	21.94	115480091
231	Phosphorylation	LRTFYQMYDKGLVYR HHHHHHHHHCCCCEE	10.05	-
233	2-Hydroxyisobutyrylation	TFYQMYDKGLVYRSY HHHHHHHCCCCEEEE	36.33	-
233	Acetylation	TFYQMYDKGLVYRSY HHHHHHHCCCCEEEE	36.33	19608861
233	Malonylation	TFYQMYDKGLVYRSY HHHHHHHCCCCEEEE	36.33	26320211
237	Phosphorylation	MYDKGLVYRSYKPVF HHHCCCCEEEECCEE	10.01	-
241	Acetylation	GLVYRSYKPVFWSPS CCCEEEECCEEECCC	35.63	19608861
241	Malonylation	GLVYRSYKPVFWSPS CCCEEEECCEEECCC	35.63	26320211
241	Methylation	GLVYRSYKPVFWSPS CCCEEEECCEEECCC	35.63	70837
241	Succinylation	GLVYRSYKPVFWSPS CCCEEEECCEEECCC	35.63	-
241	Succinylation	GLVYRSYKPVFWSPS CCCEEEECCEEECCC	35.63	-
241	Ubiquitination	GLVYRSYKPVFWSPS CCCEEEECCEEECCC	35.63	22817900
251	Phosphorylation	FWSPSSRTALAEAEL EECCCCCCHHHHHHH	28.15	21406692
260	Phosphorylation	LAEAELEYNPEHVSR HHHHHHHCCHHHCCE	48.95	21406692
266	Phosphorylation	EYNPEHVSRSIYVKF HCCHHHCCEEEEEEC	23.40	27080861
268	Phosphorylation	NPEHVSRSIYVKFPL CHHHCCEEEEEECCC	15.68	27080861
270	Phosphorylation	EHVSRSIYVKFPLLK HHCCEEEEEECCCCC	9.99	27080861
277	Acetylation	YVKFPLLKPSPKLAS EEECCCCCCCHHHHH	51.65	23236377
317	Acetylation	VCYMPESKYAVVKCS EEECCCCCEEEEEEC	35.25	25038526
322	Acetylation	ESKYAVVKCSKSGDL CCCEEEEEECCCCCE	26.62	25953088
445	2-Hydroxyisobutyrylation	EGTDVVIKMLQTAKN CCHHHHHHHHHHHHH	23.19	-
445	Ubiquitination	EGTDVVIKMLQTAKN CCHHHHHHHHHHHHH	23.19	21890473
451	Malonylation	IKMLQTAKNLLKEEK HHHHHHHHHHHHHHH	52.06	26320211
455	Sumoylation	QTAKNLLKEEKLVHS HHHHHHHHHHHCHHC	67.98	-
455	Sumoylation	QTAKNLLKEEKLVHS HHHHHHHHHHHCHHC	67.98	-
455	Ubiquitination	QTAKNLLKEEKLVHS HHHHHHHHHHHCHHC	67.98	22817900
458	Acetylation	KNLLKEEKLVHSYPY HHHHHHHHCHHCCCC	57.83	25038526
458	Ubiquitination	KNLLKEEKLVHSYPY HHHHHHHHCHHCCCC	57.83	21906983
471	Ubiquitination	PYDWRTKKPVVIRAS CCCCCCCCCEEEEEC	42.29	24816145
479	Acetylation	PVVIRASKQWFINIT CEEEEECCCEEEEHH	51.03	25825284
479	Succinylation	PVVIRASKQWFINIT CEEEEECCCEEEEHH	51.03	-
479	Succinylation	PVVIRASKQWFINIT CEEEEECCCEEEEHH	51.03	-
489	2-Hydroxyisobutyrylation	FINITDIKTAAKELL EEEHHHHHHHHHHHH	34.62	-
493	Ubiquitination	TDIKTAAKELLKKVK HHHHHHHHHHHHHCC	46.56	29967540
500	Acetylation	KELLKKVKFIPGSAL HHHHHHCCCCCHHHH	46.59	25953088
500	Succinylation	KELLKKVKFIPGSAL HHHHHHCCCCCHHHH	46.59	-
500	Succinylation	KELLKKVKFIPGSAL HHHHHHCCCCCHHHH	46.59	-
538	Ubiquitination	PIPVFHHKTKDEYLI CCCEEECCCCCCEEE	49.01	23503661
540	Acetylation	PVFHHKTKDEYLINS CEEECCCCCCEEECC	54.06	23236377
540	Malonylation	PVFHHKTKDEYLINS CEEECCCCCCEEECC	54.06	26320211
540	Ubiquitination	PVFHHKTKDEYLINS CEEECCCCCCEEECC	54.06	23503661
543	Phosphorylation	HHKTKDEYLINSQTT ECCCCCCEEECCCCH	24.02	-
547	Phosphorylation	KDEYLINSQTTEHIV CCCEEECCCCHHHHH	23.16	30576142
549	Phosphorylation	EYLINSQTTEHIVKL CEEECCCCHHHHHHH	33.69	30576142
550	Phosphorylation	YLINSQTTEHIVKLV EEECCCCHHHHHHHH	20.23	23312004
621	Phosphorylation	PDQRADLYLEGKDQL CCCCEEEEECCCCCC	11.85	-
625	Acetylation	ADLYLEGKDQLGGWF EEEEECCCCCCCHHH	32.69	25038526
649	Malonylation	ARKRAPYKTVIVHGF HHCCCCCCEEEEECE	34.94	26320211
657	Phosphorylation	TVIVHGFTLGEKGEK EEEEECEECCHHHCH	38.14	-
661	Acetylation	HGFTLGEKGEKMSKS ECEECCHHHCHHHHH	71.25	19608861
661	Malonylation	HGFTLGEKGEKMSKS ECEECCHHHCHHHHH	71.25	26320211
661	Ubiquitination	HGFTLGEKGEKMSKS ECEECCHHHCHHHHH	71.25	-
687	Acetylation	NGGQDQSKEPPYGAD CCCCCCCCCCCCCCC	69.48	25038526
687	Ubiquitination	NGGQDQSKEPPYGAD CCCCCCCCCCCCCCC	69.48	21963094
725	Acetylation	AARDDISKLRNTLRF HHHHHHHHHHHHHHH	52.37	25825284
725	Malonylation	AARDDISKLRNTLRF HHHHHHHHHHHHHHH	52.37	26320211
725	Succinylation	AARDDISKLRNTLRF HHHHHHHHHHHHHHH	52.37	27452117
725	Ubiquitination	AARDDISKLRNTLRF HHHHHHHHHHHHHHH	52.37	24816145
729	Phosphorylation	DISKLRNTLRFLLGN HHHHHHHHHHHHHHC	16.93	23828894
775	2-Hydroxyisobutyrylation	NKITELYKQYDFGKV HHHHHHHHHCCHHHH	55.81	-
775	Acetylation	NKITELYKQYDFGKV HHHHHHHHHCCHHHH	55.81	19608861
775	Succinylation	NKITELYKQYDFGKV HHHHHHHHHCCHHHH	55.81	-
775	Succinylation	NKITELYKQYDFGKV HHHHHHHHHCCHHHH	55.81	27452117
781	2-Hydroxyisobutyrylation	YKQYDFGKVVRLLRT HHHCCHHHHHHHHHH	37.23	-
781	Acetylation	YKQYDFGKVVRLLRT HHHCCHHHHHHHHHH	37.23	19608861
781	Malonylation	YKQYDFGKVVRLLRT HHHCCHHHHHHHHHH	37.23	26320211
781	Succinylation	YKQYDFGKVVRLLRT HHHCCHHHHHHHHHH	37.23	-
781	Succinylation	YKQYDFGKVVRLLRT HHHCCHHHHHHHHHH	37.23	27452117
798	Phosphorylation	TRELSNFYFSIIKDR HHHHHHHHHHHHHHH	10.73	30576142
800	Phosphorylation	ELSNFYFSIIKDRLY HHHHHHHHHHHHHHH	16.42	30576142
803	Acetylation	NFYFSIIKDRLYCEK HHHHHHHHHHHHHCC	34.82	23954790
803	Malonylation	NFYFSIIKDRLYCEK HHHHHHHHHHHHHCC	34.82	26320211
815	Ubiquitination	CEKENDPKRRSCQTA HCCCCCCCCCHHHHH	64.41	24816145
865	Phosphorylation	VFRTGWISTSSIWKK CEECCCEEHHHHHCC	18.74	28348404
866	Phosphorylation	FRTGWISTSSIWKKP EECCCEEHHHHHCCC	19.84	28348404
867	Phosphorylation	RTGWISTSSIWKKPG ECCCEEHHHHHCCCC	17.03	28348404
872	Ubiquitination	STSSIWKKPGLEEAV EHHHHHCCCCHHHHH	29.35	-
881	Phosphorylation	GLEEAVESACAMRDS CHHHHHHHHHHHCHH	23.27	20068231
883	Glutathionylation	EEAVESACAMRDSFL HHHHHHHHHHCHHHC	4.21	22555962
888	Phosphorylation	SACAMRDSFLGSIPG HHHHHCHHHCCCCCC	16.41	21712546
896	Malonylation	FLGSIPGKNAAEYKV HCCCCCCCCCCEEEE	37.75	26320211
896	Ubiquitination	FLGSIPGKNAAEYKV HCCCCCCCCCCEEEE	37.75	23503661
902	Ubiquitination	GKNAAEYKVITVIEP CCCCCEEEEEEEECC	20.75	23503661
949	Sulfoxidation	LAQEPREMTADVIEL HHCCCHHCCCCEEEE	3.85	21406390
950	Phosphorylation	AQEPREMTADVIELK HCCCHHCCCCEEEEC	18.23	20068231
957	Malonylation	TADVIELKGKFLINL CCCEEEECCEEEEEE	47.46	26320211
959	Ubiquitination	DVIELKGKFLINLEG CEEEECCEEEEEECC	35.10	-
973	Phosphorylation	GGDIREESSYKVIVM CCCCCCCCCEEEEEE	33.93	29759185
974	Phosphorylation	GDIREESSYKVIVMP CCCCCCCCEEEEEEE	32.05	29759185
975	Phosphorylation	DIREESSYKVIVMPT CCCCCCCEEEEEEEC	20.67	29759185
976	Acetylation	IREESSYKVIVMPTT CCCCCCEEEEEEECC	27.68	25038526
976	Ubiquitination	IREESSYKVIVMPTT CCCCCCEEEEEEECC	27.68	24816145
982	Phosphorylation	YKVIVMPTTKEKCPR EEEEEEECCCCCCCC	32.19	20068231
983	Phosphorylation	KVIVMPTTKEKCPRC EEEEEECCCCCCCCC	31.19	29759185
992	Acetylation	EKCPRCWKYTAESSD CCCCCCCEEECCCCC	36.03	25038526
993	Phosphorylation	KCPRCWKYTAESSDT CCCCCCEEECCCCCC	6.15	28152594
994	Phosphorylation	CPRCWKYTAESSDTL CCCCCEEECCCCCCC	21.88	28152594
1012	Ubiquitination	CAEVVSGK------- HHHHHCCC-------	50.05	24816145

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of SYIM_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SYIM_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SYIM_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
A4_HUMAN	APP	physical	21832049
GRSF1_HUMAN	GRSF1	physical	26344197
HNRH1_HUMAN	HNRNPH1	physical	26344197
HNRH2_HUMAN	HNRNPH2	physical	26344197
HNRH3_HUMAN	HNRNPH3	physical	26344197
HYAL4_HUMAN	HYAL4	physical	26344197
EPHA4_HUMAN	EPHA4	physical	28514442
DDX11_HUMAN	DDX11	physical	28514442
GAK_HUMAN	GAK	physical	28514442
STAT2_HUMAN	STAT2	physical	28514442
ESPL1_HUMAN	ESPL1	physical	28514442
ASPM_HUMAN	ASPM	physical	28514442
TMM70_HUMAN	TMEM70	physical	28514442
MTO1_HUMAN	MTO1	physical	28514442
SMG8_HUMAN	SMG8	physical	28514442
DHX40_HUMAN	DHX40	physical	28514442
BBS1_HUMAN	BBS1	physical	28514442
FANCA_HUMAN	FANCA	physical	28514442
IF122_HUMAN	IFT122	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 616007: Cataracts, growth hormone deficiency, sensory neuropathy, sensorineural hearing loss, and skeletal dysplasia (CAGSSS)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• DB00167: L-Isoleucine

Related Literatures of Post-Translational Modification

Acetylation

"Lysine acetylation targets protein complexes and co-regulates majorcellular functions.";
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.;
Science 325:834-840(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-189; LYS-233; LYS-241;LYS-775 AND LYS-781, AND MASS SPECTROMETRY.
PubMed: 19608861

Phosphorylation

"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks.";
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.;
Cell 127:635-648(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, AND MASSSPECTROMETRY.
PubMed: 17081983