dbPTM

SMG8_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SMG8_HUMAN
UniProt AC	Q8ND04
Protein Name	Protein SMG8
Gene Name	SMG8
Organism	Homo sapiens (Human).
Sequence Length	991
Subcellular Localization
Protein Description	Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG9 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required to mediate the recruitment of SMG1 to the ribosome:SURF complex and to suppress SMG1 kinase activity until the ribosome:SURF complex locates the exon junction complex (EJC). Acts as a regulator of kinase activity..
Protein Sequence	MAGPVSLRDLLMGASAWMGSESPGGSPTEGGGSAAGGPEPPWREDEICVVGIFGKTALRLNSEKFSLVNTVCDRQVFPLFRHQDPGDPGPGIRTEAGAVGEAGGAEDPGAAAGGSVRGSGAVAEGNRTEAGSQDYSLLQAYYSQESKVLYLLLTSICDNSQLLRACRALQSGEAGGGLSLPHAEAHEFWKHQEKLQCLSLLYLFSVCHILLLVHPTCSFDITYDRVFRALDGLRQKVLPLLKTAIKDCPVGKDWKLNCRPCPPRLLFLFQLNGALKVEPPRNQDPAHPDKPKKHSPKRRLQHALEDQIYRIFRKSRVLTNQSINCLFTVPANQAFVYIVPGSQEEDPVGMLLDQLRSHCTVKDPESLLVPAPLSGPRRYQVMRQHSRQQLSFHIDSSSSSSSGQLVDFTLREFLWQHVELVLSKKGFDDSVGRNPQPSHFELPTYQKWISAASKLYEVAIDGKEEDLGSPTGELTSKILSSIKVLEGFLDIDTKFSENRCQKALPMAHSAYQSNLPHNYTMTVHKNQLAQALRVYSQHARGPAFHKYAMQLHEDCYKFWSNGHQLCEERSLTDQHCVHKFHSLPKSGEKPEADRNPPVLYHNSRARSTGACNCGRKQAPRDDPFDIKAANYDFYQLLEEKCCGKLDHINFPVFEPSTPDPAPAKNESSPAPPDSDADKLKEKEPQTQGESTSLSLALSLGQSTDSLGTYPADPQAGGDNPEVHGQVEVKTEKRPNFVDRQASTVEYLPGMLHSNCPKGLLPKFSSWSLVKLGPAKSYNFHTGLDQQGFIPGTNYLMPWDIVIRTRAEDEGDLDTNSWPAPNKAIPGKRSAVVMGRGRRRDDIARAFVGFEYEDSRGRRFMCSGPDKVMKVMGSGPKESALKALNSDMPLYILSSSQGRGLKPHYAQLMRLFVVVPDAPLQIILMPQVQPGPPPCPVFYPEKQEITLPPDGLWVLRFPYAYVTERGPCFPPKENVQLMSYKVLRGVLKAVTQ

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Phosphorylation	--MAGPVSLRDLLMG --CCCCCCHHHHHHC	22.33	24043423
15	Phosphorylation	RDLLMGASAWMGSES HHHHHCCCCCCCCCC	19.41	24043423
20	Phosphorylation	GASAWMGSESPGGSP CCCCCCCCCCCCCCC	20.85	24043423
22	Phosphorylation	SAWMGSESPGGSPTE CCCCCCCCCCCCCCC	30.46	24043423
26	Phosphorylation	GSESPGGSPTEGGGS CCCCCCCCCCCCCCC	34.27	24043423
28	Phosphorylation	ESPGGSPTEGGGSAA CCCCCCCCCCCCCCC	49.30	24043423
33	Phosphorylation	SPTEGGGSAAGGPEP CCCCCCCCCCCCCCC	20.44	24043423
64	Ubiquitination	ALRLNSEKFSLVNTV HHHCCCHHCHHHHHH	39.82	29967540
64 (in isoform 2)	Ubiquitination	-	39.82	-
66	Phosphorylation	RLNSEKFSLVNTVCD HCCCHHCHHHHHHHC	42.34	21406692
66 (in isoform 2)	Phosphorylation	-	42.34	21406692
70	Phosphorylation	EKFSLVNTVCDRQVF HHCHHHHHHHCCCCC	18.29	21406692
115	Phosphorylation	PGAAAGGSVRGSGAV CCCCCCCCCCCCCCE	14.02	29978859
135	Phosphorylation	TEAGSQDYSLLQAYY CCCCCCCHHHHHHHH	8.12	22817900
141	Phosphorylation	DYSLLQAYYSQESKV CHHHHHHHHCCHHHH	7.24	-
142	Phosphorylation	YSLLQAYYSQESKVL HHHHHHHHCCHHHHH	13.59	-
146	Phosphorylation	QAYYSQESKVLYLLL HHHHCCHHHHHHHHH	22.04	-
236 (in isoform 2)	Ubiquitination	-	39.03	-
236	Ubiquitination	ALDGLRQKVLPLLKT HHHHHHHHHHHHHHH	39.03	-
242	Methylation	QKVLPLLKTAIKDCP HHHHHHHHHHHHCCC	43.52	115980877
242	Ubiquitination	QKVLPLLKTAIKDCP HHHHHHHHHHHHCCC	43.52	29967540
246	Ubiquitination	PLLKTAIKDCPVGKD HHHHHHHHCCCCCCC	51.30	29967540
252 (in isoform 2)	Ubiquitination	-	61.73	-
252	Ubiquitination	IKDCPVGKDWKLNCR HHCCCCCCCCEECCE	61.73	29967540
290	Ubiquitination	QDPAHPDKPKKHSPK CCCCCCCCCCCCCHH	64.29	29967540
309 (in isoform 2)	Phosphorylation	-	7.63	27642862
309	Phosphorylation	HALEDQIYRIFRKSR HHHHHHHHHHHHHCC	7.63	25839225
362	Ubiquitination	LRSHCTVKDPESLLV HHHCCCCCCHHHCCE	49.79	-
362 (in isoform 2)	Ubiquitination	-	49.79	-
374	Phosphorylation	LLVPAPLSGPRRYQV CCEECCCCCHHHHHH	45.77	21815630
399	Phosphorylation	FHIDSSSSSSSGQLV EEEECCCCCCCCCEE	35.87	-
409	Phosphorylation	SGQLVDFTLREFLWQ CCCEEEEEHHHHHHH	22.05	24719451
425	Ubiquitination	VELVLSKKGFDDSVG HHHHHCCCCCCCCCC	62.53	29967540
438	Phosphorylation	VGRNPQPSHFELPTY CCCCCCCCCCCCCHH	35.24	29978859
444	Phosphorylation	PSHFELPTYQKWISA CCCCCCCHHHHHHHH	51.73	29978859
445	Phosphorylation	SHFELPTYQKWISAA CCCCCCHHHHHHHHH	13.22	29978859
447	Ubiquitination	FELPTYQKWISAASK CCCCHHHHHHHHHHH	35.76	29967540
454	Ubiquitination	KWISAASKLYEVAID HHHHHHHHHHEEECC	50.81	29967540
463	Ubiquitination	YEVAIDGKEEDLGSP HEEECCCCCHHCCCC	54.93	21906983
463 (in isoform 1)	Ubiquitination	-	54.93	21890473
463 (in isoform 2)	Ubiquitination	-	54.93	21890473
463 (in isoform 3)	Ubiquitination	-	54.93	21906983
469	Phosphorylation	GKEEDLGSPTGELTS CCCHHCCCCCHHHHH	26.96	30266825
471	Phosphorylation	EEDLGSPTGELTSKI CHHCCCCCHHHHHHH	45.01	30266825
475	Phosphorylation	GSPTGELTSKILSSI CCCCHHHHHHHHHHH	24.54	30266825
476	Phosphorylation	SPTGELTSKILSSIK CCCHHHHHHHHHHHH	30.26	30266825
477	Ubiquitination	PTGELTSKILSSIKV CCHHHHHHHHHHHHH	42.29	29967540
480	Phosphorylation	ELTSKILSSIKVLEG HHHHHHHHHHHHHHC	33.23	24719451
483	Ubiquitination	SKILSSIKVLEGFLD HHHHHHHHHHHCCCC	42.59	-
494	Ubiquitination	GFLDIDTKFSENRCQ CCCCCCCCCCCCHHH	43.05	29967540
502	Ubiquitination	FSENRCQKALPMAHS CCCCHHHHHHHHHHH	56.36	29967540
536	Phosphorylation	AQALRVYSQHARGPA HHHHHHHHHHCCCHH	16.78	27251275
556	Phosphorylation	MQLHEDCYKFWSNGH HHHHHHHHHHHHCHH	22.88	-
570	Phosphorylation	HQLCEERSLTDQHCV HHHHHHHCCCCHHHH	38.61	27251275
579	Ubiquitination	TDQHCVHKFHSLPKS CCHHHHHHHHCCCCC	25.11	29967540
585	Ubiquitination	HKFHSLPKSGEKPEA HHHHCCCCCCCCCCH	76.12	29967540
586	Phosphorylation	KFHSLPKSGEKPEAD HHHCCCCCCCCCCHH	50.69	20363803
589	Ubiquitination	SLPKSGEKPEADRNP CCCCCCCCCCHHCCC	52.24	29967540
627	Ubiquitination	RDDPFDIKAANYDFY CCCCCCCCCCCCCHH	43.20	29967540
634 (in isoform 2)	Phosphorylation	-	8.71	27642862
640	Ubiquitination	FYQLLEEKCCGKLDH HHHHHHHHHCCCCCC	26.00	29967540
656	Phosphorylation	NFPVFEPSTPDPAPA CCCCCCCCCCCCCCC	45.43	30266825
657	Phosphorylation	FPVFEPSTPDPAPAK CCCCCCCCCCCCCCC	42.29	30266825
667	Phosphorylation	PAPAKNESSPAPPDS CCCCCCCCCCCCCCC	51.52	30266825
667 (in isoform 2)	Phosphorylation	-	51.52	21406692
668	Phosphorylation	APAKNESSPAPPDSD CCCCCCCCCCCCCCC	20.92	29255136
668 (in isoform 2)	Phosphorylation	-	20.92	21406692
674	Phosphorylation	SSPAPPDSDADKLKE CCCCCCCCCHHHHHH	40.24	30266825
730	Phosphorylation	HGQVEVKTEKRPNFV CCEEEEECCCCCCCC	52.49	24719451
732	Ubiquitination	QVEVKTEKRPNFVDR EEEEECCCCCCCCCC	77.66	29967540
742	Phosphorylation	NFVDRQASTVEYLPG CCCCCCCCCCCCCCC	25.34	27273156
743	Phosphorylation	FVDRQASTVEYLPGM CCCCCCCCCCCCCCC	22.00	30266825
746	Phosphorylation	RQASTVEYLPGMLHS CCCCCCCCCCCCCCC	17.54	30266825
753	Phosphorylation	YLPGMLHSNCPKGLL CCCCCCCCCCCCCCC	35.84	23403867
764	Phosphorylation	KGLLPKFSSWSLVKL CCCCCCCCCCEEEEC	36.70	-
770 (in isoform 2)	Ubiquitination	-	53.68	-
770	Ubiquitination	FSSWSLVKLGPAKSY CCCCEEEECCCCCCC	53.68	-
835	Methylation	RSAVVMGRGRRRDDI CEEEEECCCCCHHHH	19.83	12019741
869	Acetylation	SGPDKVMKVMGSGPK ECCHHHHHHHCCCCH	31.86	12653621
869	Ubiquitination	SGPDKVMKVMGSGPK ECCHHHHHHHCCCCH	31.86	29967540
876	Ubiquitination	KVMGSGPKESALKAL HHHCCCCHHHHHHHH	68.76	22817900
876	Acetylation	KVMGSGPKESALKAL HHHCCCCHHHHHHHH	68.76	12653631
876 (in isoform 2)	Ubiquitination	-	68.76	-
878	Phosphorylation	MGSGPKESALKALNS HCCCCHHHHHHHHCC	45.06	-
881 (in isoform 1)	Ubiquitination	-	51.01	21890473
881 (in isoform 2)	Ubiquitination	-	51.01	21890473
881	Ubiquitination	GPKESALKALNSDMP CCHHHHHHHHCCCCC	51.01	21890473
885	Phosphorylation	SALKALNSDMPLYIL HHHHHHCCCCCEEEE	35.82	27080861
890	Phosphorylation	LNSDMPLYILSSSQG HCCCCCEEEEECCCC	8.24	29083192
893	Phosphorylation	DMPLYILSSSQGRGL CCCEEEEECCCCCCC	20.56	25850435
894	Phosphorylation	MPLYILSSSQGRGLK CCEEEEECCCCCCCC	24.20	25850435
895	Phosphorylation	PLYILSSSQGRGLKP CEEEEECCCCCCCCH	33.02	29507054
898	Methylation	ILSSSQGRGLKPHYA EEECCCCCCCCHHHH	39.37	24129315
987	Ubiquitination	KVLRGVLKAVTQ--- HHHHHHHHHHHC---	38.10	-
1019 (in isoform 2)	Ubiquitination	-		-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of SMG8_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of SMG8_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SMG8_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
LRC49_HUMAN	LRRC49	physical	28514442
TPGS1_HUMAN	TPGS1	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SMG8_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-469, AND MASSSPECTROMETRY.	18669648 [Abstract]