dbPTM

TNKS1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	TNKS1_HUMAN
UniProt AC	O95271
Protein Name	Tankyrase-1
Gene Name	TNKS
Organism	Homo sapiens (Human).
Sequence Length	1327
Subcellular Localization	Cytoplasm. Golgi apparatus membrane Peripheral membrane protein. Chromosome, centromere. Nucleus, nuclear pore complex. Chromosome, telomere . Cytoplasm, cytoskeleton, spindle pole. Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicl
Protein Description	Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1. Stimulates 26S proteasome activity..
Protein Sequence	MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCCSTTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVSGALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELRERLTYEFKGHSLLQAAREADLAKVKKTLALEIINFKQPQSHETALHCAVASLHPKRKQVTELLLRKGANVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTLGQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEAVQQILSESTPIRTSDVDYRLLEASKAGDLETVKQLCSSQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCTPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVVSASLISPASTPSCLSAASSIDNLTGPLAELAVGGASNAGDGAAGTERKEGEVAGLDMNISQFLKSLGLEHLRDIFETEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERLLGGQQGTNPYLTFHCVNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRIQKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPEAPSQTATAAEQKT

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
44	Phosphorylation	PGLAPGTTPASPTAS CCCCCCCCCCCCCCC	23.83	-
49	Phosphorylation	GTTPASPTASGLAPF CCCCCCCCCCCCCCC	30.84	-
210	Ubiquitination	DAANVNAKDMAGRKS HHHCCCHHHHCCCCC	42.72	21890473
210	Ubiquitination	DAANVNAKDMAGRKS HHHCCCHHHHCCCCC	42.72	22817900
210 (in isoform 1)	Ubiquitination	-	42.72	21890473
210 (in isoform 2)	Ubiquitination	-	42.72	21890473
217	Phosphorylation	KDMAGRKSSPLHFAA HHHCCCCCCCCHHCC	35.51	21712546
218	Phosphorylation	DMAGRKSSPLHFAAG HHCCCCCCCCHHCCC	34.22	25627689
327	Ubiquitination	DLADPSAKAVLTGEY HHCCCCCCHHHCCCC	43.28	22817900
335	Acetylation	AVLTGEYKKDELLEA HHHCCCCCHHHHHHH	49.82	19816121
335	Ubiquitination	AVLTGEYKKDELLEA HHHCCCCCHHHHHHH	49.82	29967540
336	Ubiquitination	VLTGEYKKDELLEAA HHCCCCCHHHHHHHH	56.38	29967540
345	Phosphorylation	ELLEAARSGNEEKLM HHHHHHHHCCHHHHH	41.04	29514088
350	Ubiquitination	ARSGNEEKLMALLTP HHHCCHHHHHHHHCC	37.07	29967540
365	Phosphorylation	LNVNCHASDGRKSTP CCCCCCCCCCCCCCC	19.55	22817900
369	Ubiquitination	CHASDGRKSTPLHLA CCCCCCCCCCCCHHH	65.30	29967540
379	Phosphorylation	PLHLAAGYNRVRIVQ CCHHHCCCCHHHHHH	8.62	-
411	Phosphorylation	VPLHNACSYGHYEVT EECCCCCCCCCHHHH	31.57	22817900
412	Phosphorylation	PLHNACSYGHYEVTE ECCCCCCCCCHHHHH	14.06	22817900
470	Phosphorylation	LVNCHGKSAVDMAPT EEECCCCCCCCCCCC	37.81	24144214
477	Phosphorylation	SAVDMAPTPELRERL CCCCCCCCHHHHHHH	21.62	24719451
489	Ubiquitination	ERLTYEFKGHSLLQA HHHCEEECCHHHHHH	43.72	32015554
492	Phosphorylation	TYEFKGHSLLQAARE CEEECCHHHHHHHHH	39.17	-
507	Ubiquitination	ADLAKVKKTLALEII HCHHHHHHHHHHEEE	52.32	-
536	Ubiquitination	AVASLHPKRKQVTEL HHHHHCCCHHHHHHH	62.57	29967540
541	Phosphorylation	HPKRKQVTELLLRKG CCCHHHHHHHHHHCC	20.58	-
579	Ubiquitination	DVMEVLHKHGAKMNA HHHHHHHHCCCHHHH	39.30	23503661
583 (in isoform 2)	Ubiquitination	-	36.96	-
583	Ubiquitination	VLHKHGAKMNALDTL HHHHCCCHHHHHHHH	36.96	32015554
640	Phosphorylation	EAVQQILSESTPIRT HHHHHHHHCCCCCCC	31.00	-
659	Ubiquitination	YRLLEASKAGDLETV HHHHHHHCCCCHHHH	64.53	33845483
667	Ubiquitination	AGDLETVKQLCSSQN CCCHHHHHHHHHCCC	45.00	29967540
709	Ubiquitination	YLLHHGADVHAKDKG HHHHCCCCCCCCCCC	37.01	27667366
726	Phosphorylation	VPLHNACSYGHYEVA CCCCCCCCCCCHHHH	31.57	-
727	Phosphorylation	PLHNACSYGHYEVAE CCCCCCCCCCHHHHH	14.06	-
742	Phosphorylation	LLVRHGASVNVADLW HHHHCCCCCCHHHHH	20.86	22817900
750	Ubiquitination	VNVADLWKFTPLHEA CCHHHHHHCCHHHHH	46.81	-
760	Ubiquitination	PLHEAAAKGKYEICK HHHHHHHCCCHHHHH	51.73	32015554
762	Ubiquitination	HEAAAKGKYEICKLL HHHHHCCCHHHHHHH	38.72	22817900
767 (in isoform 1)	Ubiquitination	-	48.07	21890473
767	Ubiquitination	KGKYEICKLLLKHGA CCCHHHHHHHHHCCC	48.07	21890473
767	Ubiquitination	KGKYEICKLLLKHGA CCCHHHHHHHHHCCC	48.07	22817900
771	Ubiquitination	EICKLLLKHGADPTK HHHHHHHHCCCCCCC	39.71	22817900
777	Phosphorylation	LKHGADPTKKNRDGN HHCCCCCCCCCCCCC	56.02	-
778	Acetylation	KHGADPTKKNRDGNT HCCCCCCCCCCCCCC	54.13	11925781
779	Acetylation	HGADPTKKNRDGNTP CCCCCCCCCCCCCCC	60.74	11925791
791	Ubiquitination	NTPLDLVKEGDTDIQ CCCHHHHHCCCCCHH	64.38	32015554
812	Ubiquitination	AALLDAAKKGCLARV HHHHHHHHHCHHHHH	51.96	-
813	Ubiquitination	ALLDAAKKGCLARVQ HHHHHHHHCHHHHHH	50.56	27667366
821	Ubiquitination	GCLARVQKLCTPENI CHHHHHHHHCCCCCC	42.55	32015554
839	Phosphorylation	DTQGRNSTPLHLAAG CCCCCCCCCCEEECC	33.16	21818122
901	Ubiquitination	TCVNATDKWAFTPLH CCCCCCCCCCCCHHH	35.75	29967540
916	Phosphorylation	EAAQKGRTQLCALLL HHHHCCHHHHHHHHH	34.26	-
930	Phosphorylation	LAHGADPTMKNQEGQ HHCCCCCCCCCCCCC	41.13	21818122
932	Ubiquitination	HGADPTMKNQEGQTP CCCCCCCCCCCCCCC	58.94	-
978	Phosphorylation	VVSASLISPASTPSC EEEEHHCCCCCCHHH	21.51	22817900
982	Phosphorylation	SLISPASTPSCLSAA HHCCCCCCHHHHHHH	22.71	22817900
987	Phosphorylation	ASTPSCLSAASSIDN CCCHHHHHHHHHCCC	26.56	22817900
991	Phosphorylation	SCLSAASSIDNLTGP HHHHHHHHCCCCCCC	29.27	22817900
1020	Ubiquitination	GAAGTERKEGEVAGL CCCCCCCCCCCCCCC	64.93	-
1081	Ubiquitination	GHRHKLIKGVERLLG CHHHHHHHHHHHHHC	68.30	-
1128	Phosphorylation	VEEEMQSTIREHRDG HHHHHHHHHHHHCCC	13.73	21818122
1152	Ubiquitination	YNVIRIQKVVNKKLR HCHHHHHHHHCHHHH	45.86	29967540
1167	Ubiquitination	ERFCHRQKEVSEENH HHHHHCHHCCCCCCC	61.10	29967540
1195	Ubiquitination	FINAIIHKGFDERHA HHHHHHHCCCCHHCC	51.36	32015554
1238	Ubiquitination	GTGCPTHKDRSCYIC CCCCCCCCCCCEEEE	58.26	-
1258	Ubiquitination	FCRVTLGKSFLQFST HCHHHCCCCHHHHHH	40.94	-
1289	Phosphorylation	PSVNGLAYAEYVIYR CCCCCEEEEEEEEEC	12.66	-
1292	Phosphorylation	NGLAYAEYVIYRGEQ CCEEEEEEEEECCCC	5.51	22817900
1308	Phosphorylation	YPEYLITYQIMKPEA CCEEEEEEEECCCCC	6.77	24260401

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
839	T	Phosphorylation	Kinase	PLK1	P53350	PSP
930	T	Phosphorylation	Kinase	PLK1	P53350	PSP
978	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
978	S	Phosphorylation	Kinase	PLK1	P53350	PSP
982	T	Phosphorylation	Kinase	GSK3B	P49841	PSP
982	T	Phosphorylation	Kinase	PLK1	P53350	PSP
987	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
991	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
1128	T	Phosphorylation	Kinase	PLK1	P53350	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	RNF146	Q9NTX7	PMID:21478859

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of TNKS1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of TNKS1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TERF1_HUMAN	TERF1	physical	11739745
TB182_HUMAN	TNKS1BP1	physical	11854288
TERF1_HUMAN	TERF1	physical	11854288
TNKS1_HUMAN	TNKS	physical	9822378
TERF1_HUMAN	TERF1	physical	9822378
FACD2_HUMAN	FANCD2	physical	21314979
GMDS_HUMAN	GMDS	physical	22645305
TINF2_HUMAN	TINF2	physical	15133513
TERF1_HUMAN	TERF1	physical	15133513
TNKS1_HUMAN	TNKS	physical	15133513
AXIN1_HUMAN	AXIN1	physical	25547115
PTEN_HUMAN	PTEN	physical	25547115
TNKS1_HUMAN	TNKS	physical	25547115

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of TNKS1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column."; Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.; Anal. Sci. 24:161-166(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-365, AND MASSSPECTROMETRY.	18187866 [Abstract]