dbPTM

SRBP1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SRBP1_HUMAN
UniProt AC	P36956
Protein Name	Sterol regulatory element-binding protein 1
Gene Name	SREBF1
Organism	Homo sapiens (Human).
Sequence Length	1147
Subcellular Localization	Endoplasmic reticulum membrane Multi-pass membrane protein . Golgi apparatus membrane Multi-pass membrane protein . Cytoplasmic vesicle, COPII-coated vesicle membrane Multi-pass membrane protein . Moves from the endoplasmic reticulum to the Gol
Protein Description	Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3')..
Protein Sequence	MDEPPFSEAALEQALGEPCDLDAALLTDIEDMLQLINNQDSDFPGLFDPPYAGSGAGGTDPASPDTSSPGSLSPPPATLSSSLEAFLSGPQAAPSPLSPPQPAPTPLKMYPSMPAFSPGPGIKEESVPLSILQTPTPQPLPGALLPQSFPAPAPPQFSSTPVLGYPSPPGGFSTGSPPGNTQQPLPGLPLASPPGVPPVSLHTQVQSVVPQQLLTVTAAPTAAPVTTTVTSQIQQVPVLLQPHFIKADSLLLTAMKTDGATVKAAGLSPLVSGTTVQTGPLPTLVSGGTILATVPLVVDAEKLPINRLAAGSKAPASAQSRGEKRTAHNAIEKRYRSSINDKIIELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLSLRTAVHKSKSLKDLVSACGSGGNTDVLMEGVKTEVEDTLTPPPSDAGSPFQSSPLSLGSRGSGSGGSGSDSEPDSPVFEDSKAKPEQRPSLHSRGMLDRSRLALCTLVFLCLSCNPLASLLGARGLPSPSDTTSVYHSPGRNVLGTESRDGPGWAQWLLPPVVWLLNGLLVLVSLVLLFVYGEPVTRPHSGPAVYFWRHRKQADLDLARGDFAQAAQQLWLALRALGRPLPTSHLDLACSLLWNLIRHLLQRLWVGRWLAGRAGGLQQDCALRVDASASARDAALVYHKLHQLHTMGKHTGGHLTATNLALSALNLAECAGDAVSVATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGSVPPAMQWLCHPVGHRFFVDGDWSVLSTPWESLYSLAGNPVDPLAQVTQLFREHLLERALNCVTQPNPSPGSADGDKEFSDALGYLQLLNSCSDAAGAPAYSFSISSSMATTTGVDPVAKWWASLTAVVIHWLRRDEEAAERLCPLVEHLPRVLQESERPLPRAALHSFKAARALLGCAKAESGPASLTICEKASGYLQDSLATTPASSSIDKAVQLFLCDLLLVVRTSLWRQQQPPAPAPAAQGTSSRPQASALELRGFQRDLSSLRRLAQSFRPAMRRVFLHEATARLMAGASPTRTHQLLDRSLRRRAGPGGKGGAVAELEPRPTRREHAEALLLASCYLPPGFLSAPGQRVGMLAEAARTLEKLGDRRLLHDCQQMLMRLGGGTTVTSS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
59	Phosphorylation	AGSGAGGTDPASPDT CCCCCCCCCCCCCCC	37.58	-
63	Phosphorylation	AGGTDPASPDTSSPG CCCCCCCCCCCCCCC	28.79	22384276
98	Phosphorylation	QAAPSPLSPPQPAPT CCCCCCCCCCCCCCC	37.61	-
105	Phosphorylation	SPPQPAPTPLKMYPS CCCCCCCCCCCCCCC	43.27	-
110	Phosphorylation	APTPLKMYPSMPAFS CCCCCCCCCCCCCCC	7.20	27080861
112	Phosphorylation	TPLKMYPSMPAFSPG CCCCCCCCCCCCCCC	21.65	29978859
117	Phosphorylation	YPSMPAFSPGPGIKE CCCCCCCCCCCCCCC	30.93	25159151
123	Sumoylation	FSPGPGIKEESVPLS CCCCCCCCCCCCCEE	62.43	-
123	Sumoylation	FSPGPGIKEESVPLS CCCCCCCCCCCCCEE	62.43	-
232 (in isoform 3)	Ubiquitination	-	28.30	21906983
249	Phosphorylation	PHFIKADSLLLTAMK CCEEECCHHHHHHHC	26.27	28555341
256	Ubiquitination	SLLLTAMKTDGATVK HHHHHHHCCCCCEEE	40.79	21906983
256 (in isoform 1)	Ubiquitination	-	40.79	21906983
256 (in isoform 2)	Ubiquitination	-	40.79	21906983
275	Phosphorylation	SPLVSGTTVQTGPLP CCCCCCCEEECCCCC	17.72	-
286 (in isoform 4)	Ubiquitination	-	34.32	21906983
289 (in isoform 3)	Ubiquitination	-	19.15	21906983
312	Phosphorylation	INRLAAGSKAPASAQ CCHHHCCCCCCHHHH	22.40	-
313	Acetylation	NRLAAGSKAPASAQS CHHHCCCCCCHHHHC	58.43	25953088
313	Ubiquitination	NRLAAGSKAPASAQS CHHHCCCCCCHHHHC	58.43	21906983
313 (in isoform 1)	Ubiquitination	-	58.43	21906983
313 (in isoform 2)	Ubiquitination	-	58.43	21906983
321	Dimethylation	APASAQSRGEKRTAH CCHHHHCCCCHHHHH	45.25	-
321	Methylation	APASAQSRGEKRTAH CCHHHHCCCCHHHHH	45.25	106701399
323 (in isoform 3)	Ubiquitination	-	37.95	21906983
324	Acetylation	SAQSRGEKRTAHNAI HHHCCCCHHHHHHHH	58.85	12640139
324	Ubiquitination	SAQSRGEKRTAHNAI HHHCCCCHHHHHHHH	58.85	-
332 (in isoform 3)	Ubiquitination	-	29.13	21906983
333	2-Hydroxyisobutyrylation	TAHNAIEKRYRSSIN HHHHHHHHHHHHHHC	48.92	-
333	Acetylation	TAHNAIEKRYRSSIN HHHHHHHHHHHHHHC	48.92	12640139
337	Phosphorylation	AIEKRYRSSINDKII HHHHHHHHHHCHHHH	27.00	-
338	Phosphorylation	IEKRYRSSINDKIIE HHHHHHHHHCHHHHH	19.20	16381800
342	Ubiquitination	YRSSINDKIIELKDL HHHHHCHHHHHHHHH	40.89	-
343 (in isoform 4)	Ubiquitination	-	3.10	21906983
347	Ubiquitination	NDKIIELKDLVVGTE CHHHHHHHHHHHHCC	35.90	21906983
347 (in isoform 1)	Ubiquitination	-	35.90	21906983
347 (in isoform 2)	Ubiquitination	-	35.90	21906983
356	Ubiquitination	LVVGTEAKLNKSAVL HHHHCCCCCCHHHHH	46.49	21906983
356 (in isoform 1)	Ubiquitination	-	46.49	21906983
356 (in isoform 2)	Ubiquitination	-	46.49	21906983
359	Ubiquitination	GTEAKLNKSAVLRKA HCCCCCCHHHHHHHH	50.67	-
360	Phosphorylation	TEAKLNKSAVLRKAI CCCCCCHHHHHHHHH	23.65	29214152
365	Acetylation	NKSAVLRKAIDYIRF CHHHHHHHHHHHHHH	45.60	25953088
365	Ubiquitination	NKSAVLRKAIDYIRF CHHHHHHHHHHHHHH	45.60	-
377 (in isoform 4)	Ubiquitination	-	40.66	21906983
379	Ubiquitination	FLQHSNQKLKQENLS HHHHHCHHHHHHCHH	63.00	-
381	Ubiquitination	QHSNQKLKQENLSLR HHHCHHHHHHCHHHH	63.70	-
386	Phosphorylation	KLKQENLSLRTAVHK HHHHHCHHHHHHHHH	27.64	29449344
386 (in isoform 4)	Ubiquitination	-	27.64	21906983
389 (in isoform 4)	Ubiquitination	-	38.02	-
394	Phosphorylation	LRTAVHKSKSLKDLV HHHHHHHCCCHHHHH	16.76	28857561
395 (in isoform 4)	Ubiquitination	-	65.69	-
396	Phosphorylation	TAVHKSKSLKDLVSA HHHHHCCCHHHHHHH	47.98	28857561
398	Ubiquitination	VHKSKSLKDLVSACG HHHCCCHHHHHHHHC	56.99	-
402	Phosphorylation	KSLKDLVSACGSGGN CCHHHHHHHHCCCCC	25.74	-
409 (in isoform 4)	Ubiquitination	-	36.31	-
411 (in isoform 4)	Ubiquitination	-	35.24	-
418	Sumoylation	DVLMEGVKTEVEDTL HHHCCCCCCEEEECC	50.23	-
418	Sumoylation	DVLMEGVKTEVEDTL HHHCCCCCCEEEECC	50.23	-
419	Phosphorylation	VLMEGVKTEVEDTLT HHCCCCCCEEEECCC	42.74	28102081
424	Phosphorylation	VKTEVEDTLTPPPSD CCCEEEECCCCCCCC	21.01	28102081
425 (in isoform 4)	Ubiquitination	-	11.93	-
426	Phosphorylation	TEVEDTLTPPPSDAG CEEEECCCCCCCCCC	35.52	25159151
428 (in isoform 4)	Ubiquitination	-	35.47	-
430	Phosphorylation	DTLTPPPSDAGSPFQ ECCCCCCCCCCCCCC	45.96	16825193
434	Phosphorylation	PPPSDAGSPFQSSPL CCCCCCCCCCCCCCC	25.02	19126544
439	Phosphorylation	AGSPFQSSPLSLGSR CCCCCCCCCCCCCCC	21.05	16880739
446 (in isoform 3)	Ubiquitination	-	41.31	21906983
448	Phosphorylation	LSLGSRGSGSGGSGS CCCCCCCCCCCCCCC	28.77	25159151
450	Phosphorylation	LGSRGSGSGGSGSDS CCCCCCCCCCCCCCC	41.50	25159151
453	Phosphorylation	RGSGSGGSGSDSEPD CCCCCCCCCCCCCCC	38.35	25159151
455	Phosphorylation	SGSGGSGSDSEPDSP CCCCCCCCCCCCCCC	39.39	25159151
457	Phosphorylation	SGGSGSDSEPDSPVF CCCCCCCCCCCCCCC	53.37	25159151
461	Phosphorylation	GSDSEPDSPVFEDSK CCCCCCCCCCCCCCC	33.41	23663014
467	Phosphorylation	DSPVFEDSKAKPEQR CCCCCCCCCCCHHHC	27.58	24144214
470	Ubiquitination	VFEDSKAKPEQRPSL CCCCCCCCHHHCCCH	53.47	21906983
470 (in isoform 1)	Ubiquitination	-	53.47	21906983
470 (in isoform 2)	Ubiquitination	-	53.47	21906983
500 (in isoform 4)	Ubiquitination	-	3.82	21906983
505	Phosphorylation	LSCNPLASLLGARGL HHCCHHHHHHCCCCC	31.63	24719451
518	Phosphorylation	GLPSPSDTTSVYHSP CCCCCCCCCCEEECC	25.88	30576142
519	O-linked_Glycosylation	LPSPSDTTSVYHSPG CCCCCCCCCEEECCC	22.61	OGP
524	Phosphorylation	DTTSVYHSPGRNVLG CCCCEEECCCCCCCC	16.03	30576142
532	Phosphorylation	PGRNVLGTESRDGPG CCCCCCCCCCCCCCC	26.99	30576142
563 (in isoform 3)	Ubiquitination	-	3.59	21906983
567	Phosphorylation	SLVLLFVYGEPVTRP HHHHHHHHCCCCCCC	14.40	-
587	Ubiquitination	VYFWRHRKQADLDLA EEEEECHHHCCHHHC	43.65	21906983
587 (in isoform 1)	Ubiquitination	-	43.65	21906983
587 (in isoform 2)	Ubiquitination	-	43.65	21906983
617 (in isoform 4)	Ubiquitination	-	34.24	21906983
651 (in isoform 3)	Ubiquitination	-	21.23	21906983
675	Ubiquitination	DAALVYHKLHQLHTM HHHHHHHHHHHHHHC	29.94	21906983
675 (in isoform 1)	Ubiquitination	-	29.94	21906983
675 (in isoform 2)	Ubiquitination	-	29.94	21906983
705 (in isoform 4)	Ubiquitination	-	4.22	21906983
727	Ubiquitination	VAAALRVKTSLPRAL HHHHHHHHCCHHHHH	26.43	-
757 (in isoform 4)	Ubiquitination	-	16.78	-
900 (in isoform 3)	Ubiquitination	-	7.43	21906983
924	Methylation	RAALHSFKAARALLG HHHHHHHHHHHHHHC	44.12	24624087
924	Ubiquitination	RAALHSFKAARALLG HHHHHHHHHHHHHHC	44.12	21906983
924 (in isoform 1)	Ubiquitination	-	44.12	21906983
924 (in isoform 2)	Ubiquitination	-	44.12	21906983
934	Ubiquitination	RALLGCAKAESGPAS HHHHCHHCCCCCCCC	57.68	-
947	Ubiquitination	ASLTICEKASGYLQD CCEEEEHHHCCCCCH	43.36	-
954 (in isoform 4)	Ubiquitination	-	42.92	21906983
964 (in isoform 4)	Ubiquitination	-	32.10	-
977 (in isoform 4)	Ubiquitination	-	1.86	-
983	Phosphorylation	LLLVVRTSLWRQQQP HHHHHHHHHHHCCCC	18.51	24719451
1019	Phosphorylation	RGFQRDLSSLRRLAQ HHHHHCHHHHHHHHH	31.45	30301811
1020	Phosphorylation	GFQRDLSSLRRLAQS HHHHCHHHHHHHHHH	32.94	25954137
1027	Phosphorylation	SLRRLAQSFRPAMRR HHHHHHHHHHHHHHH	20.02	20446291
1049	Phosphorylation	ARLMAGASPTRTHQL HHHHCCCCCCHHHHH	26.36	23312004
1051	Phosphorylation	LMAGASPTRTHQLLD HHCCCCCCHHHHHHH	45.78	23312004
1053	Phosphorylation	AGASPTRTHQLLDRS CCCCCCHHHHHHHHH	19.22	26434776
1060	Phosphorylation	THQLLDRSLRRRAGP HHHHHHHHHHHHCCC	26.37	29214152
1070	Ubiquitination	RRAGPGGKGGAVAEL HHCCCCCCCCCCEEC	61.15	21906983
1070 (in isoform 1)	Ubiquitination	-	61.15	21906983
1100 (in isoform 4)	Ubiquitination	-	33.90	21906983
1121	Ubiquitination	EAARTLEKLGDRRLL HHHHHHHHHCCHHHH	61.96	2190698
1121 (in isoform 1)	Ubiquitination	-	61.96	21906983
1151 (in isoform 4)	Ubiquitination	-		21906983

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
39	S	Phosphorylation	Kinase	MAPK14	Q16539	GPS
59	T	Phosphorylation	Kinase	PKM	P14618	PSP
63	S	Phosphorylation	Kinase	P38B	Q15759	PSP
63	S	Phosphorylation	Kinase	MAPK14	Q16539	GPS
63	S	Phosphorylation	Kinase	P38G	P53778	PSP
81	T	Phosphorylation	Kinase	MAPK8	P45983	GPS
81	T	Phosphorylation	Kinase	MAPK1	P28482	GPS
93	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
93	S	Phosphorylation	Kinase	MAPK8	P45983	GPS
117	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
117	S	Phosphorylation	Kinase	MAPK3	P27361	GPS
117	S	Phosphorylation	Kinase	MAPK8	P45983	GPS
117	S	Phosphorylation	Kinase	JNK2	P45984	PSP
314	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
337	S	Phosphorylation	Kinase	SIK1	P57059	Uniprot
338	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
338	S	Phosphorylation	Kinase	SIK1	P57059	Uniprot
372	S	Phosphorylation	Kinase	PRKAA1	Q13131	GPS
396	S	Phosphorylation	Kinase	AMPK	Q9Y478	Uniprot
402	T	Phosphorylation	Kinase	MAPK14	Q16539	GPS
402	S	Phosphorylation	Kinase	SIK1	P57059	Uniprot
426	T	Phosphorylation	Kinase	P38B	Q15759	PSP
426	T	Phosphorylation	Kinase	P38G	P53778	PSP
426	T	Phosphorylation	Kinase	MAPK14	Q16539	GPS
426	T	Phosphorylation	Kinase	GSK3B	P49841	PSP
430	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
434	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
439	S	Phosphorylation	Kinase	CDK1	P06493	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	FBXW7	Q969H0	PMID:24658274

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
402	S	Phosphorylation		-
Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SRBP1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TWST2_HUMAN	TWIST2	physical	14654692
UBC9_HUMAN	UBE2I	physical	12615929
CBP_HUMAN	CREBBP	physical	8918891
SP1_HUMAN	SP1	physical	10224053
NR0B1_HUMAN	NR0B1	physical	11713202
EP300_HUMAN	EP300	physical	12640139
ANDR_HUMAN	AR	physical	18245227
SP1_HUMAN	SP1	physical	10235267
EP300_HUMAN	EP300	physical	10235267
CBP_HUMAN	CREBBP	physical	10235267
MED15_HUMAN	MED15	physical	10235267
MED23_HUMAN	MED23	physical	10235267
MED7_HUMAN	MED7	physical	10235267
MED13_HUMAN	MED13	physical	10235267
MED12_HUMAN	MED12	physical	10235267
MED1_HUMAN	MED1	physical	10235267
MED14_HUMAN	MED14	physical	10235267
MED24_HUMAN	MED24	physical	10235267
MED26_HUMAN	MED26	physical	10235267
MED6_HUMAN	MED6	physical	10235267
CBP_HUMAN	CREBBP	physical	9765204
HNF4A_HUMAN	HNF4A	physical	17636037
MED15_HUMAN	MED15	physical	20534441
TRRAP_HUMAN	TRRAP	physical	20534441
KAT2A_HUMAN	KAT2A	physical	20534441
ATM_HUMAN	ATM	physical	20534441
EP300_HUMAN	EP300	physical	20534441
GCN1_HUMAN	GCN1L1	physical	20534441
TAD2B_HUMAN	TADA2B	physical	20534441
TADA3_HUMAN	TADA3	physical	20534441
RUVB2_HUMAN	RUVBL2	physical	20534441
MED12_HUMAN	MED12	physical	11834832
MED13_HUMAN	MED13	physical	11834832
MED23_HUMAN	MED23	physical	11834832
MED14_HUMAN	MED14	physical	11834832
MED15_HUMAN	MED15	physical	11834832
MED24_HUMAN	MED24	physical	11834832
MED25_HUMAN	MED25	physical	11834832
MED17_HUMAN	MED17	physical	11834832
MED26_HUMAN	MED26	physical	11834832
MED7_HUMAN	MED7	physical	11834832
MED6_HUMAN	MED6	physical	11834832
MED21_HUMAN	MED21	physical	11834832
CDK8_HUMAN	CDK8	physical	11834832
FBXW7_HUMAN	FBXW7	physical	19126544
CBP_HUMAN	CREBBP	physical	16799563
MED15_HUMAN	MED15	physical	16799563
MED1_HUMAN	MED1	physical	16799563
CDK8_HUMAN	CDK8	physical	16799563
MED6_HUMAN	MED6	physical	16799563
MED13_HUMAN	MED13	physical	16799563
MED12_HUMAN	MED12	physical	16799563
MED14_HUMAN	MED14	physical	16799563
MED23_HUMAN	MED23	physical	16799563
MED24_HUMAN	MED24	physical	16799563
MED25_HUMAN	MED25	physical	16799563
MED17_HUMAN	MED17	physical	16799563
MED26_HUMAN	MED26	physical	16799563
SRBP2_HUMAN	SREBF2	physical	15798184
SRBP1_HUMAN	SREBF1	physical	15798184
ZBT7A_HUMAN	ZBTB7A	physical	18682402
LMNA_HUMAN	LMNA	physical	19727227
EGF_HUMAN	EGF	physical	19727227
NDF1_RAT	Neurod1	physical	16055439
SIR1_HUMAN	SIRT1	physical	20817729
ID2_HUMAN	ID2	physical	10585876
ID3_HUMAN	ID3	physical	10585876
ANM5_HUMAN	PRMT5	physical	26759235
FBXW7_HUMAN	FBXW7	physical	26759235
PRVA_HUMAN	PVALB	physical	28514442
SORL_HUMAN	SORL1	physical	28514442
PLK1_HUMAN	PLK1	physical	27579997
FBXW7_HUMAN	FBXW7	physical	27579997
RPB1_HUMAN	POLR2A	physical	29033323

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SRBP1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-117, AND MASSSPECTROMETRY.	18669648 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1019 AND SER-1020, ANDMASS SPECTROMETRY.	17081983 [Abstract]

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