ARHG4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ARHG4_HUMAN
• UniProt AC: Q9NR80
• Protein Name: Rho guanine nucleotide exchange factor 4
• Gene Name: ARHGEF4
• Organism: Homo sapiens (Human).
• Sequence Length: 690
• Subcellular Localization: Isoform 3: Cytoplasm. Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Associated with membrane ruffles.
• Protein Description: Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression..
• Protein Sequence: MPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
62	Phosphorylation	PRGIPHRSPVSVDDL CCCCCCCCCCCHHHH	26.98	25159151
65	Phosphorylation	IPHRSPVSVDDLWLE CCCCCCCCHHHHHHH	24.18	25159151
73	Ubiquitination	VDDLWLEKTQRKKLQ HHHHHHHHHHHHHHH	48.03	-
105	Phosphorylation	GVKCWRKTIITSPES CCEEEEEEEECCHHH	14.72	27050516
108	Phosphorylation	CWRKTIITSPESLNL EEEEEEECCHHHCCC	34.09	25159151
109	Phosphorylation	WRKTIITSPESLNLP EEEEEECCHHHCCCC	19.09	27050516
112	Phosphorylation	TIITSPESLNLPRRS EEECCHHHCCCCCCC	26.41	25159151
165	Phosphorylation	VGSEEDLYDDLHSSS CCCHHHHHHHHHCCC	21.52	-
175	Phosphorylation	LHSSSHHYSHPGGGG HHCCCCCCCCCCCCC	11.84	-
279	Phosphorylation	DGGAEAQSSKDQMRT CCCCCCCCCHHHHHH	46.53	-
280	Phosphorylation	GGAEAQSSKDQMRTN CCCCCCCCHHHHHHH	28.33	-
286	Phosphorylation	SSKDQMRTNVINEIL CCHHHHHHHHHHHHH	28.61	-
328	Phosphorylation	FSEEQLRTIFGNIED CCHHHHHHHHCCHHH	29.29	24719451
624	Ubiquitination	SITELQRKQAMLNAS CHHHHHHHHHHHHHH	29.46	-
632	Ubiquitination	QAMLNASKQQVTGKP HHHHHHHHHHHCCCC	42.19	-
644 (in isoform 4)	Phosphorylation	-	16.12	26657352
655 (in isoform 4)	Phosphorylation	-	31.65	25404012
663 (in isoform 4)	Phosphorylation	-	37.72	25404012
667 (in isoform 4)	Phosphorylation	-	5.10	25404012
670 (in isoform 4)	Phosphorylation	-	34.87	25404012

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ARHG4_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ARHG4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ARHG4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
APC_HUMAN	APC	physical	10947987
RHOA_HUMAN	RHOA	physical	10947987
RAC1_HUMAN	RAC1	physical	10947987
1B07_HUMAN	HLA-B	physical	20936779
1B18_HUMAN	HLA-B	physical	20936779

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.