dbPTM

PHLP1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PHLP1_HUMAN
UniProt AC	O60346
Protein Name	PH domain leucine-rich repeat-containing protein phosphatase 1
Gene Name	PHLPP1
Organism	Homo sapiens (Human).
Sequence Length	1717
Subcellular Localization	Cytoplasm. Membrane Peripheral membrane protein. Nucleus. In colorectal cancer tissue, expression is concentrated at the lateral membrane of epithelial cells. Isoform 2: Cell membrane .
Protein Description	Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA. [PubMed: 15808505]
Protein Sequence	MEPAAAATVQRLPELGREDRASAPAAAAAAAAAAAAAAAALAAAAGGGRSPEPALTPAAPSGGNGSGSGAREEAPGEAPPGPLPGRAGGAGRRRRRGAPQPIAGGAAPVPGAGGGANSLLLRRGRLKRNLSAAAAAASSSSSSSAAAASHSPGAAGLPASCSASASLCTRSLDRKTLLLKHRQTLQLQPSDRDWVRHQLQRGCVHVFDRHMASTYLRPVLCTLDTTAGEVAARLLQLGHKGGGVVKVLGQGPGAAAAREPAEPPPEAGPRLAPPEPRDSEVPPARSAPGAFGGPPRAPPADLPLPVGGPGGWSRRASPAPSDSSPGEPFVGGPVSSPRAPRPVVSDTESFSLSPSAESVSDRLDPYSSGGGSSSSSEELEADAASAPTGVPGQPRRPGHPAQPLPLPQTASSPQPQQKAPRAIDSPGGAVREGSCEEKAAAAVAPGGLQSTPGRSGVTAEKAPPPPPPPTLYVQLHGETTRRLEAEEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPKLQELDLTGNPRLVLDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGGSFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGAVPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPPGALSENSPAYPSEQRCMLHPICLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFTNGSRVEVEVDIHCSRAKEKEKQQHLLQVPAEASDEGIVISANEDEPGLPRKADFSAVGTIGRRRANGSVAPQERSHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQPPPPPQLQPQLPRHYQLDQLPDYYDTPL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEPAAAAT -------CCHHHHHH	53.63	22814378
50	Phosphorylation	AAAGGGRSPEPALTP HHHCCCCCCCCCCCC	36.57	28348404
56	Phosphorylation	RSPEPALTPAAPSGG CCCCCCCCCCCCCCC	17.00	28348404
118	Phosphorylation	GAGGGANSLLLRRGR CCCCCHHHHHHHCCH	21.62	25850435
131	Phosphorylation	GRLKRNLSAAAAAAS CHHHHHHHHHHHHHH	21.29	28450419
138	Phosphorylation	SAAAAAASSSSSSSA HHHHHHHHCCCHHHH	26.48	27080861
139	Phosphorylation	AAAAAASSSSSSSAA HHHHHHHCCCHHHHH	29.52	29970186
140	Phosphorylation	AAAAASSSSSSSAAA HHHHHHCCCHHHHHH	31.57	29970186
141	Phosphorylation	AAAASSSSSSSAAAA HHHHHCCCHHHHHHH	35.87	29970186
142	Phosphorylation	AAASSSSSSSAAAAS HHHHCCCHHHHHHHH	30.20	29970186
143	Phosphorylation	AASSSSSSSAAAASH HHHCCCHHHHHHHHC	26.41	25627689
144	Phosphorylation	ASSSSSSSAAAASHS HHCCCHHHHHHHHCC	24.71	-
149	Phosphorylation	SSSAAAASHSPGAAG HHHHHHHHCCCCCCC	21.55	25627689
151	Phosphorylation	SAAAASHSPGAAGLP HHHHHHCCCCCCCCC	23.64	25627689
169	Phosphorylation	SASASLCTRSLDRKT CCCHHHHHCCCCCCH	28.69	-
171	Phosphorylation	SASLCTRSLDRKTLL CHHHHHCCCCCCHHH	19.23	30576142
176	Phosphorylation	TRSLDRKTLLLKHRQ HCCCCCCHHHHHCCC	24.43	30576142
184	Phosphorylation	LLLKHRQTLQLQPSD HHHHCCCCCCCCCCC	19.21	21406692
190	Phosphorylation	QTLQLQPSDRDWVRH CCCCCCCCCHHHHHH	32.11	21406692
222	O-linked_Glycosylation	YLRPVLCTLDTTAGE HHHCEEEECCCCHHH	24.41	OGP
240	Ubiquitination	RLLQLGHKGGGVVKV HHHHCCCCCCCEEEE	58.41	30230243
246	Ubiquitination	HKGGGVVKVLGQGPG CCCCCEEEECCCCCC	29.36	21890473
279	Phosphorylation	APPEPRDSEVPPARS CCCCCCCCCCCCCCC	41.60	-
313	Phosphorylation	VGGPGGWSRRASPAP CCCCCCCCCCCCCCC	18.37	24719451
317	Phosphorylation	GGWSRRASPAPSDSS CCCCCCCCCCCCCCC	21.56	29255136
321	Phosphorylation	RRASPAPSDSSPGEP CCCCCCCCCCCCCCC	52.83	21815630
323	Phosphorylation	ASPAPSDSSPGEPFV CCCCCCCCCCCCCCC	42.65	29255136
324	Phosphorylation	SPAPSDSSPGEPFVG CCCCCCCCCCCCCCC	41.75	29255136
335	Phosphorylation	PFVGGPVSSPRAPRP CCCCCCCCCCCCCCC	37.45	29255136
336	Phosphorylation	FVGGPVSSPRAPRPV CCCCCCCCCCCCCCC	20.74	29255136
409	Phosphorylation	QPLPLPQTASSPQPQ CCCCCCCCCCCCCCC	26.94	23663014
411	Phosphorylation	LPLPQTASSPQPQQK CCCCCCCCCCCCCCC	46.03	25159151
412	Phosphorylation	PLPQTASSPQPQQKA CCCCCCCCCCCCCCC	25.70	25159151
425	Phosphorylation	KAPRAIDSPGGAVRE CCCCCCCCCCCCCCC	21.23	30266825
434	Phosphorylation	GGAVREGSCEEKAAA CCCCCCCCHHHHHHH	17.38	22210691
450	Phosphorylation	VAPGGLQSTPGRSGV HCCCCCCCCCCCCCC	41.43	25159151
451	Phosphorylation	APGGLQSTPGRSGVT CCCCCCCCCCCCCCC	19.46	21815630
524	Phosphorylation	IGCLIRFYAGKPHST HHEEEEEECCCCCCC	12.47	22210691
534	Phosphorylation	KPHSTGSSERIQLSG CCCCCCCCCCEEECC	32.04	22210691
691	Phosphorylation	LNELQRFTKLKSLNL HHHHHHHHHCHHCCC	37.34	22210691
797	O-linked_Glycosylation	MSGNCVETLRLQALR HCCCHHHHHHHHHHH	9.22	30379171
1071	Ubiquitination	EIDLSGNKLKAIPTT EECCCCCCCCCCCHH	55.69	29967540
1077	Phosphorylation	NKLKAIPTTIMNCRR CCCCCCCHHHHHHHH	23.88	23403867
1078	Phosphorylation	KLKAIPTTIMNCRRM CCCCCCHHHHHHHHC	16.93	23403867
1146	Ubiquitination	PRLVLDHKTLELLNN CEEEECHHHHHHHHC	55.24	22817900
1147	O-linked_Glycosylation	RLVLDHKTLELLNNI EEEECHHHHHHHHCC	23.26	30379171
1206	Phosphorylation	CDNREALYGVFDGDR CCCHHHHHCEECCCC	20.49	27642862
1242	Phosphorylation	KTKNEEEYMVNTFIV HCCCHHHHHHHHHHH	15.05	27732954
1246	Phosphorylation	EEEYMVNTFIVMQRK HHHHHHHHHHHHHHH	11.88	27732954
1323	Ubiquitination	LKRIKQHKAIITEDG HHHHHHCCEEECCCC	38.49	29967540
1359	Phosphorylation	VPRPHVQSVLLTPQD CCCCCCCEEEECCCC	17.40	19797085
1363	Phosphorylation	HVQSVLLTPQDEFFI CCCEEEECCCCEEEE	18.10	19797085
1379	Phosphorylation	GSKGLWDSLSVEEAV CCCCHHHCCCHHHHH	15.80	19797085
1381	Phosphorylation	KGLWDSLSVEEAVEA CCHHHCCCHHHHHHH	31.28	19797085
1400	Ubiquitination	PDALAAAKKLCTLAQ HHHHHHHHHHHHHHH	41.36	29967540
1524	Phosphorylation	MLHPICLSNSFQRQL CCCHHHHCHHHHHHH	26.47	28450419
1526	Phosphorylation	HPICLSNSFQRQLSS CHHHHCHHHHHHHHH	21.27	28450419
1617	Phosphorylation	ADFSAVGTIGRRRAN CCCCCCCCCCCCCCC	17.41	29255136
1651	Phosphorylation	PLRKPGGYFAAPAQP CCCCCCCCCCCCCCC	8.92	27642862
1704	Phosphorylation	QPQLPRHYQLDQLPD CCCCCCCCCHHHCCC	16.43	27642862
1712	Phosphorylation	QLDQLPDYYDTPL-- CHHHCCCCCCCCC--	10.99	19060867
1713	Phosphorylation	LDQLPDYYDTPL--- HHHCCCCCCCCC---	22.26	27642862

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
1359	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
1359	S	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
1363	T	Phosphorylation	Kinase	GSK3B	P49841	PSP
1363	T	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
1379	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
1379	S	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
1381	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
1381	S	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
-	K	Ubiquitination	E3 ubiquitin ligase	BTRC	Q9Y297	PMID:24658274

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of PHLP1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PHLP1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FBW1A_HUMAN	BTRC	physical	19797085
FKBP5_HUMAN	FKBP5	physical	19615732
PPP5_HUMAN	PPP5C	physical	19615732
UBP1_HUMAN	USP1	physical	19615732
NHRF2_HUMAN	SLC9A3R2	physical	19615732
SGT1_HUMAN	SUGT1	physical	19615732
PHLP2_HUMAN	PHLPP2	physical	19615732
SCRIB_HUMAN	SCRIB	physical	19615732
WDR48_HUMAN	WDR48	physical	19615732
RBM15_HUMAN	RBM15	physical	19615732
UBP46_HUMAN	USP46	physical	19615732
SNX27_HUMAN	SNX27	physical	19615732
WDR20_HUMAN	WDR20	physical	19615732
UBP12_HUMAN	USP12	physical	19615732
AKT1_HUMAN	AKT1	physical	20605778
UBP46_HUMAN	USP46	physical	22391563
UBP1_HUMAN	USP1	physical	22426999
SNX6_HUMAN	SNX6	physical	22939629
BUD23_HUMAN	WBSCR22	physical	22939629
AKT1_HUMAN	AKT1	physical	15808505
UBP12_HUMAN	USP12	physical	25216524
WDR48_HUMAN	WDR48	physical	25216524
WDR20_HUMAN	WDR20	physical	25216524
NHRF1_HUMAN	SLC9A3R1	physical	21804599
BAG2_HUMAN	BAG2	physical	27880917
FKBP4_HUMAN	FKBP4	physical	27880917
FKBP5_HUMAN	FKBP5	physical	27880917
MCMBP_HUMAN	MCMBP	physical	27880917
NUDC3_HUMAN	NUDCD3	physical	27880917
PHLP2_HUMAN	PHLPP2	physical	27880917
2AAA_HUMAN	PPP2R1A	physical	27880917
2ABA_HUMAN	PPP2R2A	physical	27880917
RPAP3_HUMAN	RPAP3	physical	27880917
NHRF2_HUMAN	SLC9A3R2	physical	27880917
SGT1_HUMAN	SUGT1	physical	27880917
UBP1_HUMAN	USP1	physical	27880917
UBP12_HUMAN	USP12	physical	27880917
UBP46_HUMAN	USP46	physical	27880917
WDR20_HUMAN	WDR20	physical	27880917
WDR48_HUMAN	WDR48	physical	27880917
AIP_HUMAN	AIP	physical	27880917
CBPC1_HUMAN	AGTPBP1	physical	27880917
NUDC_HUMAN	NUDC	physical	27880917
RBM15_HUMAN	RBM15	physical	27880917
GLMN_HUMAN	GLMN	physical	27880917
FANCI_HUMAN	FANCI	physical	27097374
AKT1_HUMAN	AKT1	physical	27097374
WDR48_HUMAN	WDR48	physical	24145035
CHIP_HUMAN	STUB1	physical	24145035
COA1_HUMAN	COA1	physical	24145035
SGT1_HUMAN	SUGT1	physical	24145035
UBP12_HUMAN	USP12	physical	24145035
UBP1_HUMAN	USP1	physical	24145035
UBP46_HUMAN	USP46	physical	24145035
SCRIB_HUMAN	SCRIB	physical	24145035
IRS4_HUMAN	IRS4	physical	24145035

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PHLP1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Time-resolved mass spectrometry of tyrosine phosphorylation sites inthe epidermal growth factor receptor signaling network reveals dynamicmodules."; Zhang Y., Wolf-Yadlin A., Ross P.L., Pappin D.J., Rush J.,Lauffenburger D.A., White F.M.; Mol. Cell. Proteomics 4:1240-1250(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1712, AND MASSSPECTROMETRY.	15951569 [Abstract]