KANK1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: KANK1_HUMAN
• UniProt AC: Q14678
• Protein Name: KN motif and ankyrin repeat domain-containing protein 1
• Gene Name: KANK1
• Organism: Homo sapiens (Human).
• Sequence Length: 1352
• Subcellular Localization: Cell projection, ruffle membrane . Colocalizes with KIF21A in membrane ruffles.; Isoform 1: Cytoplasm. Nucleus. Shuttles between the cytoplasm and nucleus.; Isoform 2: Cytoplasm. Nucleus. Cell projection, ruffle membrane. Shuttles between t
• Protein Description: Involved in the control of cytoskeleton formation by regulating actin polymerization. Inhibits actin fiber formation and cell migration. Inhibits RhoA activity; the function involves phosphorylation through PI3K/Akt signaling and may depend on the competetive interaction with 14-3-3 adapter proteins to sequester them from active complexes. Inhibits the formation of lamellipodia but not of filopodia; the function may depend on the competetive interaction with BAIAP2 to block its association with activated RAC1. Inhibits fibronectin-mediated cell spreading; the function is partially mediated by BAIAP2. Inhibits neurite outgrowth. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. In the nucleus, is involved in beta-catenin-dependent activation of transcription. Potential tumor suppressor for renal cell carcinoma..
• Protein Sequence: MAHTTKVNGSASGKAGDILSGDQDKEQKDPYFVETPYGYQLDLDFLKYVDDIQKGNTIKRLNIQKRRKPSVPCPEPRTTSGQQGIWTSTESLSSSNSDDNKQCPNFLIARSQVTSTPISKPPPPLETSLPFLTIPENRQLPPPSPQLPKHNLHVTKTLMETRRRLEQERATMQMTPGEFRRPRLASFGGMGTTSSLPSFVGSGNHNPAKHQLQNGYQGNGDYGSYAPAAPTTSSMGSSIRHSPLSSGISTPVTNVSPMHLQHIREQMAIALKRLKELEEQVRTIPVLQVKISVLQEEKRQLVSQLKNQRAASQINVCGVRKRSYSAGNASQLEQLSRARRSGGELYIDYEEEEMETVEQSTQRIKEFRQLTADMQALEQKIQDSSCEASSELRENGECRSVAVGAEENMNDIVVYHRGSRSCKDAAVGTLVEMRNCGVSVTEAMLGVMTEADKEIELQQQTIESLKEKIYRLEVQLRETTHDREMTKLKQELQAAGSRKKVDKATMAQPLVFSKVVEAVVQTRDQMVGSHMDLVDTCVGTSVETNSVGISCQPECKNKVVGPELPMNWWIVKERVEMHDRCAGRSVEMCDKSVSVEVSVCETGSNTEESVNDLTLLKTNLNLKEVRSIGCGDCSVDVTVCSPKECASRGVNTEAVSQVEAAVMAVPRTADQDTSTDLEQVHQFTNTETATLIESCTNTCLSTLDKQTSTQTVETRTVAVGEGRVKDINSSTKTRSIGVGTLLSGHSGFDRPSAVKTKESGVGQININDNYLVGLKMRTIACGPPQLTVGLTASRRSVGVGDDPVGESLENPQPQAPLGMMTGLDHYIERIQKLLAEQQTLLAENYSELAEAFGEPHSQMGSLNSQLISTLSSINSVMKSASTEELRNPDFQKTSLGKITGNYLGYTCKCGGLQSGSPLSSQTSQPEQEVGTSEGKPISSLDAFPTQEGTLSPVNLTDDQIAAGLYACTNNESTLKSIMKKKDGNKDSNGAKKNLQFVGINGGYETTSSDDSSSDESSSSESDDECDVIEYPLEEEEEEEDEDTRGMAEGHHAVNIEGLKSARVEDEMQVQECEPEKVEIRERYELSEKMLSACNLLKNTINDPKALTSKDMRFCLNTLQHEWFRVSSQKSAIPAMVGDYIAAFEAISPDVLRYVINLADGNGNTALHYSVSHSNFEIVKLLLDADVCNVDHQNKAGYTPIMLAALAAVEAEKDMRIVEELFGCGDVNAKASQAGQTALMLAVSHGRIDMVKGLLACGADVNIQDDEGSTALMCASEHGHVEIVKLLLAQPGCNGHLEDNDGSTALSIALEAGHKDIAVLLYAHVNFAKAQSPGTPRLGRKTSPGPTHRGSFD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
13 (in isoform 2)	Phosphorylation	-	24.45	29507054
20	Phosphorylation	GKAGDILSGDQDKEQ CCCCCCCCCCCCCCC	40.43	46163855
37	Phosphorylation	PYFVETPYGYQLDLD CCEEECCCCEEEEHH	35.57	27642862
54	Ubiquitination	KYVDDIQKGNTIKRL HHHHHHHCCCEEEEC	55.23	29967540
70	Phosphorylation	IQKRRKPSVPCPEPR CCCCCCCCCCCCCCC	41.55	26657352
87	Phosphorylation	SGQQGIWTSTESLSS CCCCCEEECCHHCCC	24.69	28348404
88	Phosphorylation	GQQGIWTSTESLSSS CCCCEEECCHHCCCC	18.38	24719451
89	Phosphorylation	QQGIWTSTESLSSSN CCCEEECCHHCCCCC	23.40	28348404
114	Phosphorylation	LIARSQVTSTPISKP EEEECCCCCCCCCCC	21.17	28348404
115	Phosphorylation	IARSQVTSTPISKPP EEECCCCCCCCCCCC	33.07	28348404
116	Phosphorylation	ARSQVTSTPISKPPP EECCCCCCCCCCCCC	18.44	21712546
119	Phosphorylation	QVTSTPISKPPPPLE CCCCCCCCCCCCCCC	40.07	24719451
127	Phosphorylation	KPPPPLETSLPFLTI CCCCCCCCCCCCEEC	43.15	28348404
128	Phosphorylation	PPPPLETSLPFLTIP CCCCCCCCCCCEECC	25.35	21712546
133	Phosphorylation	ETSLPFLTIPENRQL CCCCCCEECCCCCCC	34.56	46163859
144	Phosphorylation	NRQLPPPSPQLPKHN CCCCCCCCCCCCCCC	30.93	30576142
148	Ubiquitination	PPPSPQLPKHNLHVT CCCCCCCCCCCCHHH	30.52	29967540
167	Phosphorylation	ETRRRLEQERATMQM HHHHHHHHHHHHHCC	50.49	33259812
171	Phosphorylation	RLEQERATMQMTPGE HHHHHHHHHCCCCCC	17.73	25002506
175	Phosphorylation	ERATMQMTPGEFRRP HHHHHCCCCCCCCCC	15.96	63781323
186	Phosphorylation	FRRPRLASFGGMGTT CCCCCCCCCCCCCCC	29.08	29255136
192	Phosphorylation	ASFGGMGTTSSLPSF CCCCCCCCCCCCCCC	18.02	23403867
193	Phosphorylation	SFGGMGTTSSLPSFV CCCCCCCCCCCCCCC	15.58	23403867
194	Phosphorylation	FGGMGTTSSLPSFVG CCCCCCCCCCCCCCC	29.70	23403867
195	Phosphorylation	GGMGTTSSLPSFVGS CCCCCCCCCCCCCCC	42.05	23403867
198	Phosphorylation	GTTSSLPSFVGSGNH CCCCCCCCCCCCCCC	37.55	23403867
202	Phosphorylation	SLPSFVGSGNHNPAK CCCCCCCCCCCCHHH	30.99	23403867
216	Phosphorylation	KHQLQNGYQGNGDYG HHHHCCCCCCCCCCC	22.31	21406692
222	Phosphorylation	GYQGNGDYGSYAPAA CCCCCCCCCCCCCCC	14.90	21406692
224	Phosphorylation	QGNGDYGSYAPAAPT CCCCCCCCCCCCCCC	16.34	21406692
225	Phosphorylation	GNGDYGSYAPAAPTT CCCCCCCCCCCCCCC	16.55	21406692
231	Phosphorylation	SYAPAAPTTSSMGSS CCCCCCCCCCCCCCC	34.28	21406692
232	Phosphorylation	YAPAAPTTSSMGSSI CCCCCCCCCCCCCCC	20.11	21406692
233	Phosphorylation	APAAPTTSSMGSSIR CCCCCCCCCCCCCCC	22.32	21406692
234	Phosphorylation	PAAPTTSSMGSSIRH CCCCCCCCCCCCCCC	25.68	21406692
237	Phosphorylation	PTTSSMGSSIRHSPL CCCCCCCCCCCCCCC	17.60	21406692
238	Phosphorylation	TTSSMGSSIRHSPLS CCCCCCCCCCCCCCC	20.22	21406692
242	Phosphorylation	MGSSIRHSPLSSGIS CCCCCCCCCCCCCCC	20.07	29255136
245	Phosphorylation	SIRHSPLSSGISTPV CCCCCCCCCCCCCCC	29.87	23663014
246	Phosphorylation	IRHSPLSSGISTPVT CCCCCCCCCCCCCCC	48.07	23663014
249	Phosphorylation	SPLSSGISTPVTNVS CCCCCCCCCCCCCCC	30.13	23663014
250	Phosphorylation	PLSSGISTPVTNVSP CCCCCCCCCCCCCCH	21.62	23663014
253	Phosphorylation	SGISTPVTNVSPMHL CCCCCCCCCCCHHHH	30.98	23663014
256	Phosphorylation	STPVTNVSPMHLQHI CCCCCCCCHHHHHHH	20.69	23663014
283	Phosphorylation	ELEEQVRTIPVLQVK HHHHHHCCCCHHHHH	30.67	50565381
306	Ubiquitination	RQLVSQLKNQRAASQ HHHHHHHHHHHHHHH	43.32	29967540
308	Ubiquitination	LVSQLKNQRAASQIN HHHHHHHHHHHHHCC	34.50	29967540
312	Phosphorylation	LKNQRAASQINVCGV HHHHHHHHHCCCCCC	30.52	28555341
323	Phosphorylation	VCGVRKRSYSAGNAS CCCCCCCCCCCCCHH	26.86	29255136
324	Phosphorylation	CGVRKRSYSAGNASQ CCCCCCCCCCCCHHH	13.43	22167270
325	Phosphorylation	GVRKRSYSAGNASQL CCCCCCCCCCCHHHH	30.83	19664994
330	Phosphorylation	SYSAGNASQLEQLSR CCCCCCHHHHHHHHH	39.02	22167270
336	Phosphorylation	ASQLEQLSRARRSGG HHHHHHHHHHHHCCC	24.48	23403867
341	Phosphorylation	QLSRARRSGGELYID HHHHHHHCCCCEEEE	45.67	41443325
371	Phosphorylation	IKEFRQLTADMQALE HHHHHHHHHHHHHHH	16.57	28348404
385	Phosphorylation	EQKIQDSSCEASSEL HHHHHHCCCHHCHHH	25.02	21815630
429	Phosphorylation	CKDAAVGTLVEMRNC HHHCHHHHHHHHHHC	22.49	46163865
461	Phosphorylation	EIELQQQTIESLKEK HHHHHHHHHHHHHHH	23.52	27732954
464	Phosphorylation	LQQQTIESLKEKIYR HHHHHHHHHHHHHHH	40.67	27732954
466	Ubiquitination	QQTIESLKEKIYRLE HHHHHHHHHHHHHHH	67.26	29967540
479	Phosphorylation	LEVQLRETTHDREMT HHHHHHCCCCCHHHH	24.01	68723863
480	Phosphorylation	EVQLRETTHDREMTK HHHHHCCCCCHHHHH	19.61	22210691
505	Phosphorylation	RKKVDKATMAQPLVF CCCCCHHHHCCHHHH	20.84	29083192
513	Phosphorylation	MAQPLVFSKVVEAVV HCCHHHHHHHHHHHH	19.63	29083192
522	Phosphorylation	VVEAVVQTRDQMVGS HHHHHHHCHHHHCCC	25.30	50565421
529	Phosphorylation	TRDQMVGSHMDLVDT CHHHHCCCCHHHCCC	12.29	50565373
544	Phosphorylation	CVGTSVETNSVGISC CCCCCCCCCCCEEEC	30.55	50565397
546	Phosphorylation	GTSVETNSVGISCQP CCCCCCCCCEEECCH	29.19	50565413
585	Phosphorylation	HDRCAGRSVEMCDKS CCHHCCCCHHHCCCC	22.85	50565365
592	Phosphorylation	SVEMCDKSVSVEVSV CHHHCCCCEEEEEEE	13.33	50565389
641	Phosphorylation	SVDVTVCSPKECASR EEEEEEECHHHHHHC	34.37	25627689
714	Phosphorylation	TSTQTVETRTVAVGE CCCCEEEEEEEEECC	27.57	22210691
733	Phosphorylation	DINSSTKTRSIGVGT ECCCCCCEEEEECCH	29.72	22798277
734	Ubiquitination	INSSTKTRSIGVGTL CCCCCCEEEEECCHH	28.03	29967540
735	Phosphorylation	NSSTKTRSIGVGTLL CCCCCEEEEECCHHH	29.00	22798277
756	Phosphorylation	DRPSAVKTKESGVGQ CCCCCEEECCCCCCE	33.18	46163877
770	Phosphorylation	QININDNYLVGLKMR EEECCCCEEEEEEEE	12.96	11185067
778	Phosphorylation	LVGLKMRTIACGPPQ EEEEEEEEEEECCCE	14.93	20068231
791	Phosphorylation	PQLTVGLTASRRSVG CEEEEEEEECCCCCC	19.36	28857561
793	Phosphorylation	LTVGLTASRRSVGVG EEEEEEECCCCCCCC	24.00	20068231
796	Phosphorylation	GLTASRRSVGVGDDP EEEECCCCCCCCCCC	23.32	20068231
879	Phosphorylation	SINSVMKSASTEELR HHHHHHHHCCHHHHH	14.89	23401153
881	Phosphorylation	NSVMKSASTEELRNP HHHHHHCCHHHHHCC	43.36	30266825
882	Phosphorylation	SVMKSASTEELRNPD HHHHHCCHHHHHCCC	32.94	30266825
892	Ubiquitination	LRNPDFQKTSLGKIT HHCCCCCCCCHHHCC	39.82	29967540
916	Phosphorylation	CGGLQSGSPLSSQTS CCCCCCCCCCCCCCC	28.10	25627689
930	Ubiquitination	SQPEQEVGTSEGKPI CCCHHHCCCCCCCCC	23.83	29967540
946	Ubiquitination	SLDAFPTQEGTLSPV CCCCCCCCCCCCCCC	47.57	29967540
1086	Phosphorylation	IRERYELSEKMLSAC HHHHHHHHHHHHHHH	24.19	24114839
1088	Ubiquitination	ERYELSEKMLSACNL HHHHHHHHHHHHHHH	41.59	29967540
1091	Phosphorylation	ELSEKMLSACNLLKN HHHHHHHHHHHHHHH	27.55	28857561
1104	Ubiquitination	KNTINDPKALTSKDM HHHCCCHHHCCHHHH	59.91	29967540
1231	Phosphorylation	GDVNAKASQAGQTAL CCCCCCHHHHHHHHH	21.61	22210691
1236	Phosphorylation	KASQAGQTALMLAVS CHHHHHHHHHHHHHH	22.35	-
1331	Phosphorylation	VNFAKAQSPGTPRLG HHHHHCCCCCCCCCC	30.11	22199227
1334	Phosphorylation	AKAQSPGTPRLGRKT HHCCCCCCCCCCCCC	14.50	22199227
1341	Phosphorylation	TPRLGRKTSPGPTHR CCCCCCCCCCCCCCC	38.50	23927012
1342	Phosphorylation	PRLGRKTSPGPTHRG CCCCCCCCCCCCCCC	30.78	28985074
1346	Phosphorylation	RKTSPGPTHRGSFD- CCCCCCCCCCCCCC-	30.07	23927012
1350	Phosphorylation	PGPTHRGSFD----- CCCCCCCCCC-----	26.63	23927012

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
325	S	Phosphorylation	Kinase	PKB	P31749	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of KANK1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of KANK1_HUMAN !!

Protein-Protein Interaction

Oops, there are no PPI records of KANK1_HUMAN !!

Drug and Disease Associations

• Kegg Disease
• H01097: Cerebral palsy
• OMIM Disease
• 612900: Cerebral palsy, spastic quadriplegic 2 (CPSQ2)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions.";
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.;
Sci. Signal. 2:RA46-RA46(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-325, AND MASSSPECTROMETRY.
PubMed: 19690332