GLU2B_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GLU2B_HUMAN
• UniProt AC: P14314
• Protein Name: Glucosidase 2 subunit beta
• Gene Name: PRKCSH
• Organism: Homo sapiens (Human).
• Sequence Length: 528
• Subcellular Localization: Endoplasmic reticulum .
• Protein Description: Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins..
• Protein Sequence: MLLPLLLLLPMCWAVEVKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRLKKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYRSEALPTDLPAPSAPDLTEPKEEQPPVPSSPTEEEEEEEEEEEEEAEEEEEEEDSEEAPPPLSPPQPASPAEEDKMPPYDEQTQAFIDAAQEARNKFEEAERSLKDMEESIRNLEQEISFDFGPNGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
24	Phosphorylation	VKRPRGVSLTNHHFY CCCCCCCCCCCCCCC	32.09	25849741
26	Phosphorylation	RPRGVSLTNHHFYDE CCCCCCCCCCCCCCC	25.62	26133373
31	Phosphorylation	SLTNHHFYDESKPFT CCCCCCCCCCCCCCC	17.91	28348404
72	N-linked_Glycosylation	PGTAACPNGSFHCTN CCCCCCCCCCEEECC	59.64	UniProtKB CARBOHYD
83	Ubiquitination	HCTNTGYKPLYIPSN EECCCCCCCCEECCC	29.86	-
83	Methylation	HCTNTGYKPLYIPSN EECCCCCCCCEECCC	29.86	115975685
89	Phosphorylation	YKPLYIPSNRVNDGV CCCCEECCCCCCCCC	27.92	-
117	Acetylation	VICENTCKEKGRKER CCCCCHHHHCCHHHH	61.49	26051181
126	Phosphorylation	KGRKERESLQQMAEV CCHHHHHHHHHHHHH	37.39	25159151
130	Sulfoxidation	ERESLQQMAEVTREG HHHHHHHHHHHHHHH	1.90	21406390
134	O-linked_Glycosylation	LQQMAEVTREGFRLK HHHHHHHHHHHHHHH	17.95	55826287
142	2-Hydroxyisobutyrylation	REGFRLKKILIEDWK HHHHHHHHHHHHHHH	47.54	-
142	Ubiquitination	REGFRLKKILIEDWK HHHHHHHHHHHHHHH	47.54	-
149	2-Hydroxyisobutyrylation	KILIEDWKKAREEKQ HHHHHHHHHHHHHHH	49.21	-
149	Succinylation	KILIEDWKKAREEKQ HHHHHHHHHHHHHHH	49.21	23954790
149	Acetylation	KILIEDWKKAREEKQ HHHHHHHHHHHHHHH	49.21	27452117
158	2-Hydroxyisobutyrylation	AREEKQKKLIELQAG HHHHHHHHHHHHHHC	52.80	-
166	Succinylation	LIELQAGKKSLEDQV HHHHHHCCCCHHHHH	42.05	-
166	Acetylation	LIELQAGKKSLEDQV HHHHHHCCCCHHHHH	42.05	25953088
166	Ubiquitination	LIELQAGKKSLEDQV HHHHHHCCCCHHHHH	42.05	-
166	Succinylation	LIELQAGKKSLEDQV HHHHHHCCCCHHHHH	42.05	23954790
166	2-Hydroxyisobutyrylation	LIELQAGKKSLEDQV HHHHHHCCCCHHHHH	42.05	-
167	2-Hydroxyisobutyrylation	IELQAGKKSLEDQVE HHHHHCCCCHHHHHH	60.39	-
167	Ubiquitination	IELQAGKKSLEDQVE HHHHHCCCCHHHHHH	60.39	-
168	Phosphorylation	ELQAGKKSLEDQVEM HHHHCCCCHHHHHHH	40.24	28355574
175	Sulfoxidation	SLEDQVEMLRTVKEE CHHHHHHHHHHHHHH	3.01	28465586
177	Methylation	EDQVEMLRTVKEEAE HHHHHHHHHHHHHHH	35.84	115488789
180	Succinylation	VEMLRTVKEEAEKPE HHHHHHHHHHHHCHH	49.58	23954790
196	2-Hydroxyisobutyrylation	EAKEQHQKLWEEQLA HHHHHHHHHHHHHHH	54.14	-
196	Ubiquitination	EAKEQHQKLWEEQLA HHHHHHHHHHHHHHH	54.14	-
206	Ubiquitination	EEQLAAAKAQQEQEL HHHHHHHHHHHHHHH	41.84	-
228	Phosphorylation	LDDDMDGTVSVTELQ CCCCCCCEEEEEEEE	12.82	26074081
230	Phosphorylation	DDMDGTVSVTELQTH CCCCCEEEEEEEECC	24.14	26074081
232	Phosphorylation	MDGTVSVTELQTHPE CCCEEEEEEEECCCC	24.38	26074081
263	O-linked_Glycosylation	LLSGDTQTDATSFYD HHCCCCCCCHHHHHH	29.69	OGP
280	Phosphorylation	WAAIRDKYRSEALPT HHHHHHHHHHCCCCC	24.54	26657352
282	O-linked_Glycosylation	AIRDKYRSEALPTDL HHHHHHHHCCCCCCC	24.91	23301498
282	Phosphorylation	AIRDKYRSEALPTDL HHHHHHHHCCCCCCC	24.91	26657352
287	O-linked_Glycosylation	YRSEALPTDLPAPSA HHHCCCCCCCCCCCC	51.52	23301498
287	Phosphorylation	YRSEALPTDLPAPSA HHHCCCCCCCCCCCC	51.52	26657352
293	O-linked_Glycosylation	PTDLPAPSAPDLTEP CCCCCCCCCCCCCCC	55.32	23301498
293	Phosphorylation	PTDLPAPSAPDLTEP CCCCCCCCCCCCCCC	55.32	25693802
298	O-linked_Glycosylation	APSAPDLTEPKEEQP CCCCCCCCCCCCCCC	58.59	23301498
298	Phosphorylation	APSAPDLTEPKEEQP CCCCCCCCCCCCCCC	58.59	25693802
335	Phosphorylation	EEEEEEDSEEAPPPL HHHHHHCCCCCCCCC	40.39	25137130
343	O-linked_Glycosylation	EEAPPPLSPPQPASP CCCCCCCCCCCCCCC	40.06	OGP
343	Phosphorylation	EEAPPPLSPPQPASP CCCCCCCCCCCCCCC	40.06	25137130
349	Phosphorylation	LSPPQPASPAEEDKM CCCCCCCCCCCCCCC	31.18	-
349	O-linked_Glycosylation	LSPPQPASPAEEDKM CCCCCCCCCCCCCCC	31.18	OGP
363	O-linked_Glycosylation	MPPYDEQTQAFIDAA CCCCCHHHHHHHHHH	21.59	OGP
376	Ubiquitination	AAQEARNKFEEAERS HHHHHHHHHHHHHHH	48.91	-
376	Acetylation	AAQEARNKFEEAERS HHHHHHHHHHHHHHH	48.91	26822725
376	2-Hydroxyisobutyrylation	AAQEARNKFEEAERS HHHHHHHHHHHHHHH	48.91	-
383	Phosphorylation	KFEEAERSLKDMEES HHHHHHHHHHHHHHH	31.14	-
385	Ubiquitination	EEAERSLKDMEESIR HHHHHHHHHHHHHHH	57.16	-
385	Succinylation	EEAERSLKDMEESIR HHHHHHHHHHHHHHH	57.16	23954790
385	2-Hydroxyisobutyrylation	EEAERSLKDMEESIR HHHHHHHHHHHHHHH	57.16	-
387	Sulfoxidation	AERSLKDMEESIRNL HHHHHHHHHHHHHHH	6.18	21406390
390	Phosphorylation	SLKDMEESIRNLEQE HHHHHHHHHHHHHHH	17.34	26546556
423	Phosphorylation	YELTTNEYVYRLCPF EECCCCCHHHHHCCH	12.59	25884760
431	Acetylation	VYRLCPFKLVSQKPK HHHHCCHHHCCCCCC	32.87	25953088
434	Phosphorylation	LCPFKLVSQKPKLGG HCCHHHCCCCCCCCC	42.81	21082442
435	Ubiquitination	CPFKLVSQKPKLGGS CCHHHCCCCCCCCCC	59.60	21890473
436	Ubiquitination	PFKLVSQKPKLGGSP CHHHCCCCCCCCCCC	36.29	-
438	Ubiquitination	KLVSQKPKLGGSPTS HHCCCCCCCCCCCCC	67.79	21890473
442	Phosphorylation	QKPKLGGSPTSLGTW CCCCCCCCCCCCCCC	24.17	28450419
444	Phosphorylation	PKLGGSPTSLGTWGS CCCCCCCCCCCCCCC	37.98	27251275
445	Phosphorylation	KLGGSPTSLGTWGSW CCCCCCCCCCCCCCC	28.34	27251275
448	Phosphorylation	GSPTSLGTWGSWIGP CCCCCCCCCCCCCCC	31.76	28450419
451	Phosphorylation	TSLGTWGSWIGPDHD CCCCCCCCCCCCCCC	13.69	-
459	Acetylation	WIGPDHDKFSAMKYE CCCCCCCCCCCCEEE	37.49	26051181
459	2-Hydroxyisobutyrylation	WIGPDHDKFSAMKYE CCCCCCCCCCCCEEE	37.49	-
459	Ubiquitination	WIGPDHDKFSAMKYE CCCCCCCCCCCCEEE	37.49	-
464	Ubiquitination	HDKFSAMKYEQGTGC CCCCCCCEEECCCCC	45.40	-
464	Acetylation	HDKFSAMKYEQGTGC CCCCCCCEEECCCCC	45.40	27452117
465	Phosphorylation	DKFSAMKYEQGTGCW CCCCCCEEECCCCCC	10.62	28152594
471	Glutathionylation	KYEQGTGCWQGPNRS EEECCCCCCCCCCCC	2.17	22555962
476	N-linked_Glycosylation	TGCWQGPNRSTTVRL CCCCCCCCCCCCEEE	58.31	UniProtKB CARBOHYD
484	Ubiquitination	RSTTVRLLCGKETMV CCCCEEEECCCCEEC	2.25	21890473
485	Glutathionylation	STTVRLLCGKETMVT CCCEEEECCCCEECC	9.66	22555962
487	Ubiquitination	TVRLLCGKETMVTST CEEEECCCCEECCCC	49.36	2189047
487	2-Hydroxyisobutyrylation	TVRLLCGKETMVTST CEEEECCCCEECCCC	49.36	-
490	Sulfoxidation	LLCGKETMVTSTTEP EECCCCEECCCCCCH	3.04	21406390
494	Phosphorylation	KETMVTSTTEPSRCE CCEECCCCCCHHHHH	26.30	21712546
498	Phosphorylation	VTSTTEPSRCEYLME CCCCCCHHHHHHHHH	43.24	21712546

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
24	S	Phosphorylation	Kinase	FAM20C	Q8IXL6	Uniprot
89	S	Phosphorylation	Kinase	PKC	-	Uniprot
168	S	Phosphorylation	Kinase	FAM20C	Q8IXL6	Uniprot
383	S	Phosphorylation	Kinase	PKC	-	Uniprot
390	S	Phosphorylation	Kinase	PKC	-	Uniprot
434	S	Phosphorylation	Kinase	PKC	-	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of GLU2B_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GLU2B_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NU153_HUMAN	NUP153	physical	22939629
PNPT1_HUMAN	PNPT1	physical	22939629
PDIA1_HUMAN	P4HB	physical	22939629
RS6_HUMAN	RPS6	physical	21988832
VDR_HUMAN	VDR	physical	21988832
ARMX3_HUMAN	ARMCX3	physical	22863883
BUB1B_HUMAN	BUB1B	physical	22863883
PUR6_HUMAN	PAICS	physical	22863883
RNBP6_HUMAN	RANBP6	physical	22863883
DNA2_HUMAN	DNA2	physical	26186194
APC7_HUMAN	ANAPC7	physical	26186194
PREP_HUMAN	PITRM1	physical	26186194
TSC2_HUMAN	TSC2	physical	26186194
PEX14_HUMAN	PEX14	physical	26186194
TBA1A_HUMAN	TUBA1A	physical	26186194
S27A2_HUMAN	SLC27A2	physical	26186194
BBS1_HUMAN	BBS1	physical	26186194
AURKA_HUMAN	AURKA	physical	26186194
TBB3_HUMAN	TUBB3	physical	26186194
COPG1_HUMAN	COPG1	physical	26186194
INT7_HUMAN	INTS7	physical	26186194
DEN6A_HUMAN	DENND6A	physical	26186194
FUK_HUMAN	FUK	physical	26186194
IPO9_HUMAN	IPO9	physical	26344197
FUK_HUMAN	FUK	physical	28514442
TSC2_HUMAN	TSC2	physical	28514442
DEN6A_HUMAN	DENND6A	physical	28514442
PEX14_HUMAN	PEX14	physical	28514442
TBA4A_HUMAN	TUBA4A	physical	28514442
AURKA_HUMAN	AURKA	physical	28514442
EFNB3_HUMAN	EFNB3	physical	28514442
TBB3_HUMAN	TUBB3	physical	28514442
PREP_HUMAN	PITRM1	physical	28514442
S27A2_HUMAN	SLC27A2	physical	28514442
COPG1_HUMAN	COPG1	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 174050: Polycystic liver disease (PCLD)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.