AP1B1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AP1B1_HUMAN
• UniProt AC: Q10567
• Protein Name: AP-1 complex subunit beta-1
• Gene Name: AP1B1
• Organism: Homo sapiens (Human).
• Sequence Length: 949
• Subcellular Localization: Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
• Protein Description: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules..
• Protein Sequence: MTDSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASMTVGKDVSALFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKTAAVCVAKLHDINAQLVEDQGFLDTLKDLISDSNPMVVANAVAALSEIAESHPSSNLLDLNPQSINKLLTALNECTEWGQIFILDCLANYMPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFMEMLSKDLDYYGTLLKKLAPPLVTLLSAEPELQYVALRNINLIVQKRPEILKHEMKVFFVKYNDPIYVKLEKLDIMIRLASQANIAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIKDIFRKYPNKYESVIATLCENLDSLDEPEARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLQLLTAIVKLFLKKPTETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVAAKEVVLAEKPLISEETDLIEPTLLDELICYIGTLASVYHKPPSAFVEGGRGVVHKSLPPRTASSESAESPETAPTGAPPGEQPDVIPAQGDLLGDLLNLDLGPPVSGPPLATSSVQMGAVDLLGGGLDSLMGDEPEGIGGTNFVAPPTAAVPANLGAPIGSGLSDLFDLTSGVGTLSGSYVAPKAVWLPAMKAKGLEISGTFTRQVGSISMDLQLTNKALQVMTDFAIQFNRNSFGLAPATPLQVHAPLSPNQTVEISLPLSTVGSVMKMEPLNNLQVAVKNNIDVFYFSTLYPLHILFVEDGKMDRQMFLATWKDIPNENEAQFQIRDCPLNAEAASSKLQSSNIFTVAKRNVEGQDMLYQSLKLTNGIWVLAELRIQPGNPSCTDLELSLKCRAPEVSQHVYQAYETILKN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MTDSKYFTT ------CCCCCCEEC	49.32	17192257
4	Phosphorylation	----MTDSKYFTTTK ----CCCCCCEECCC	21.79	25002506
5	Ubiquitination	---MTDSKYFTTTKK ---CCCCCCEECCCC	47.39	23000965
5 (in isoform 3)	Ubiquitination	-	47.39	-
6	Phosphorylation	--MTDSKYFTTTKKG --CCCCCCEECCCCC	15.31	29759185
8	Phosphorylation	MTDSKYFTTTKKGEI CCCCCCEECCCCCEE	30.39	28060719
9	Phosphorylation	TDSKYFTTTKKGEIF CCCCCEECCCCCEEE	27.07	28857561
10	Phosphorylation	DSKYFTTTKKGEIFE CCCCEECCCCCEEEE	27.92	28857561
11	2-Hydroxyisobutyrylation	SKYFTTTKKGEIFEL CCCEECCCCCEEEEE	57.60	-
11	Ubiquitination	SKYFTTTKKGEIFEL CCCEECCCCCEEEEE	57.60	23000965
12	Ubiquitination	KYFTTTKKGEIFELK CCEECCCCCEEEEEE	60.73	33845483
12 (in isoform 3)	Malonylation	-	60.73	26320211
12 (in isoform 3)	Ubiquitination	-	60.73	-
24	Phosphorylation	ELKAELNSDKKEKKK EEECHHCCCHHHHHH	64.53	50565767
31	Ubiquitination	SDKKEKKKEAVKKVI CCHHHHHHHHHHHHH	63.07	-
35	Ubiquitination	EKKKEAVKKVIASMT HHHHHHHHHHHHHHC	48.66	-
40	Phosphorylation	AVKKVIASMTVGKDV HHHHHHHHHCCCCCH	12.12	25954137
42	Phosphorylation	KKVIASMTVGKDVSA HHHHHHHCCCCCHHH	24.50	25954137
60	Ubiquitination	DVVNCMQTDNLELKK HHHHHHCCCCHHHHH	10.44	29901268
70	Phosphorylation	LELKKLVYLYLMNYA HHHHHHHHHHHHHHH	10.48	24260401
74	Ubiquitination	KLVYLYLMNYAKSQP HHHHHHHHHHHHHCC	1.97	33845483
76	Phosphorylation	VYLYLMNYAKSQPDM HHHHHHHHHHHCCCC	11.05	24260401
79	Phosphorylation	YLMNYAKSQPDMAIM HHHHHHHHCCCCEEH	38.48	20068231
90	Phosphorylation	MAIMAVNTFVKDCED CEEHHHHHHHHHCCC	24.17	46164897
90	Ubiquitination	MAIMAVNTFVKDCED CEEHHHHHHHHHCCC	24.17	32015554
95	Glutathionylation	VNTFVKDCEDPNPLI HHHHHHHCCCCCHHH	5.46	22555962
95	S-palmitoylation	VNTFVKDCEDPNPLI HHHHHHHCCCCCHHH	5.46	26865113
117	Ubiquitination	MGCIRVDKITEYLCE CCCEEHHHHHHHHHH	48.98	21890473
117	Ubiquitination	MGCIRVDKITEYLCE CCCEEHHHHHHHHHH	48.98	22817900
117 (in isoform 1)	Ubiquitination	-	48.98	21890473
117 (in isoform 2)	Ubiquitination	-	48.98	21890473
117 (in isoform 3)	Ubiquitination	-	48.98	-
117	Ubiquitination	MGCIRVDKITEYLCE CCCEEHHHHHHHHHH	48.98	21890473
117	Ubiquitination	MGCIRVDKITEYLCE CCCEEHHHHHHHHHH	48.98	21890473
119	Phosphorylation	CIRVDKITEYLCEPL CEEHHHHHHHHHHHH	25.07	28152594
121	Phosphorylation	RVDKITEYLCEPLRK EHHHHHHHHHHHHHH	14.17	28152594
123	Glutathionylation	DKITEYLCEPLRKCL HHHHHHHHHHHHHHC	5.04	22555962
128	Ubiquitination	YLCEPLRKCLKDEDP HHHHHHHHHCCCCCH	52.15	23503661
129	Glutathionylation	LCEPLRKCLKDEDPY HHHHHHHHCCCCCHH	4.64	22555962
131	Ubiquitination	EPLRKCLKDEDPYVR HHHHHHCCCCCHHHH	69.42	33845483
131 (in isoform 3)	Ubiquitination	-	69.42	-
136	Phosphorylation	CLKDEDPYVRKTAAV HCCCCCHHHHHHHHH	25.80	82609
138	Methylation	KDEDPYVRKTAAVCV CCCCHHHHHHHHHHH	25.43	-
139	Ubiquitination	DEDPYVRKTAAVCVA CCCHHHHHHHHHHHH	32.26	27667366
147	Ubiquitination	TAAVCVAKLHDINAQ HHHHHHHHHHHHCHH	26.42	32015554
245	Phosphorylation	QSICERVTPRLSHAN HHHHHHHCCCCCCCC	14.65	15994073
249	Phosphorylation	ERVTPRLSHANSAVV HHHCCCCCCCCHHHH	23.10	25332170
258	Phosphorylation	ANSAVVLSAVKVLMK CCHHHHHHHHHHHHH	21.29	25332170
272	Ubiquitination	KFMEMLSKDLDYYGT HHHHHHHCCCCHHHH	59.35	22817900
272 (in isoform 1)	Ubiquitination	-	59.35	21890473
272 (in isoform 2)	Ubiquitination	-	59.35	21890473
276	Phosphorylation	MLSKDLDYYGTLLKK HHHCCCCHHHHHHHH	15.87	26074081
277	Phosphorylation	LSKDLDYYGTLLKKL HHCCCCHHHHHHHHH	11.87	26074081
279	Phosphorylation	KDLDYYGTLLKKLAP CCCCHHHHHHHHHHC	16.88	30815383
282	Ubiquitination	DYYGTLLKKLAPPLV CHHHHHHHHHHCCHH	49.18	21890473
282	2-Hydroxyisobutyrylation	DYYGTLLKKLAPPLV CHHHHHHHHHHCCHH	49.18	-
282	Ubiquitination	DYYGTLLKKLAPPLV CHHHHHHHHHHCCHH	49.18	21890473
282 (in isoform 1)	Ubiquitination	-	49.18	21890473
282 (in isoform 2)	Ubiquitination	-	49.18	21890473
282	Ubiquitination	DYYGTLLKKLAPPLV CHHHHHHHHHHCCHH	49.18	21890473
282	Ubiquitination	DYYGTLLKKLAPPLV CHHHHHHHHHHCCHH	49.18	21890473
300	Phosphorylation	SAEPELQYVALRNIN CCCHHHHHHHHHCCC	10.58	110749201
318	Acetylation	QKRPEILKHEMKVFF ECCHHHHCCCEEEEE	43.08	19608861
322	Acetylation	EILKHEMKVFFVKYN HHHCCCEEEEEEECC	32.67	22424773
328	Phosphorylation	MKVFFVKYNDPIYVK EEEEEEECCCCEEEE	21.35	28152594
333	Phosphorylation	VKYNDPIYVKLEKLD EECCCCEEEEHHHHH	9.27	110749209
335	Ubiquitination	YNDPIYVKLEKLDIM CCCCEEEEHHHHHHH	33.68	21906983
335 (in isoform 1)	Ubiquitination	-	33.68	21890473
335 (in isoform 2)	Ubiquitination	-	33.68	21890473
359	Ubiquitination	AQVLAELKEYATEVD HHHHHHHHHHHCCCC	39.94	16196087
361	Phosphorylation	VLAELKEYATEVDVD HHHHHHHHHCCCCHH	19.75	20068231
363	Phosphorylation	AELKEYATEVDVDFV HHHHHHHCCCCHHHH	35.32	20068231
383	Ubiquitination	AIGRCAIKVEQSAER HHHHHEEEHHHHHHH	22.61	24816145
383 (in isoform 3)	Ubiquitination	-	22.61	-
393	Phosphorylation	QSAERCVSTLLDLIQ HHHHHHHHHHHHHHH	19.57	28857561
394	Phosphorylation	SAERCVSTLLDLIQT HHHHHHHHHHHHHHH	15.79	29083192
401	Phosphorylation	TLLDLIQTKVNYVVQ HHHHHHHHHHHHHHH	29.93	29083192
481	Ubiquitination	HDESTQVQLQLLTAI CCCCHHHHHHHHHHH	17.42	33845483
497	Phosphorylation	KLFLKKPTETQELVQ HHHCCCCCHHHHHHH	61.62	29978859
499	Phosphorylation	FLKKPTETQELVQQV HCCCCCHHHHHHHHH	29.80	29978859
508	Phosphorylation	ELVQQVLSLATQDSD HHHHHHHHHHHCCCC	19.66	29978859
511	Phosphorylation	QQVLSLATQDSDNPD HHHHHHHHCCCCCCC	37.90	29978859
514	Phosphorylation	LSLATQDSDNPDLRD HHHHHCCCCCCCHHH	29.48	29978859
524	Phosphorylation	PDLRDRGYIYWRLLS CCHHHHCEEEEEECC	7.54	7458789
531	Phosphorylation	YIYWRLLSTDPVAAK EEEEEECCCCCCCHH	35.04	28450419
532	Phosphorylation	IYWRLLSTDPVAAKE EEEEECCCCCCCHHH	44.27	28450419
534	Ubiquitination	WRLLSTDPVAAKEVV EEECCCCCCCHHHHH	20.10	29901268
538	Ubiquitination	STDPVAAKEVVLAEK CCCCCCHHHHHHCCC	41.10	33845483
574	Nitrated tyrosine	IGTLASVYHKPPSAF HHHHHHHHCCCCHHH	11.13	-
574	Nitration	IGTLASVYHKPPSAF HHHHHHHHCCCCHHH	11.13	-
591	Ubiquitination	GGRGVVHKSLPPRTA CCCCCEECCCCCCCC	41.92	23000965
592	Phosphorylation	GRGVVHKSLPPRTAS CCCCEECCCCCCCCC	31.09	24719451
597	Phosphorylation	HKSLPPRTASSESAE ECCCCCCCCCCCCCC	36.34	18423515
599	Phosphorylation	SLPPRTASSESAESP CCCCCCCCCCCCCCC	33.74	24275569
600	Phosphorylation	LPPRTASSESAESPE CCCCCCCCCCCCCCC	32.86	18423525
602	Phosphorylation	PRTASSESAESPETA CCCCCCCCCCCCCCC	39.01	18423535
605	Phosphorylation	ASSESAESPETAPTG CCCCCCCCCCCCCCC	27.96	69013251
661	Ubiquitination	GAVDLLGGGLDSLMG CCHHHHCCCHHHHCC	33.20	33845483
666	Ubiquitination	LGGGLDSLMGDEPEG HCCCHHHHCCCCCCC	4.43	33845483
673	Ubiquitination	LMGDEPEGIGGTNFV HCCCCCCCCCCCCCC	34.26	33845483
723	Ubiquitination	YVAPKAVWLPAMKAK EECCCEEEHHHHHCC	11.07	33845483
727	Sulfoxidation	KAVWLPAMKAKGLEI CEEEHHHHHCCCCEE	4.02	30846556
728	Acetylation	AVWLPAMKAKGLEIS EEEHHHHHCCCCEEE	49.40	25953088
730	Ubiquitination	WLPAMKAKGLEISGT EHHHHHCCCCEEEEE	59.52	33845483
735	Phosphorylation	KAKGLEISGTFTRQV HCCCCEEEEEEECCC	23.54	22210691
739	Phosphorylation	LEISGTFTRQVGSIS CEEEEEEECCCCCEE	21.74	69213769
786	Phosphorylation	LQVHAPLSPNQTVEI EEEECCCCCCCEEEE	22.27	7968539
790	Phosphorylation	APLSPNQTVEISLPL CCCCCCCEEEEEEEC	27.09	45741499
849	Ubiquitination	DRQMFLATWKDIPNE CHHHEEEECCCCCCC	34.88	29967540
860	Ubiquitination	IPNENEAQFQIRDCP CCCCCCHHHEEECCC	25.30	21890473
860	Ubiquitination	IPNENEAQFQIRDCP CCCCCCHHHEEECCC	25.30	21890473
869	Ubiquitination	QIRDCPLNAEAASSK EEECCCCCHHHHHHH	22.37	29967540
869 (in isoform 3)	Ubiquitination	-	22.37	-
876	Ubiquitination	NAEAASSKLQSSNIF CHHHHHHHCHHCCCE	48.31	29967540
879	Phosphorylation	AASSKLQSSNIFTVA HHHHHCHHCCCEEEE	35.47	46164879
880	Ubiquitination	ASSKLQSSNIFTVAK HHHHCHHCCCEEEEE	22.45	21890473
880	Phosphorylation	ASSKLQSSNIFTVAK HHHHCHHCCCEEEEE	22.45	46164885
880	Ubiquitination	ASSKLQSSNIFTVAK HHHHCHHCCCEEEEE	22.45	21890473
880 (in isoform 2)	Ubiquitination	-	22.45	21890473
880 (in isoform 3)	Malonylation	-	22.45	26320211
880 (in isoform 3)	Ubiquitination	-	22.45	-
880	Ubiquitination	ASSKLQSSNIFTVAK HHHHCHHCCCEEEEE	22.45	21890473
887	2-Hydroxyisobutyrylation	SNIFTVAKRNVEGQD CCCEEEEECCCCCHH	39.92	-
887	Ubiquitination	SNIFTVAKRNVEGQD CCCEEEEECCCCCHH	39.92	27667366
887 (in isoform 1)	Ubiquitination	-	39.92	21890473
897	Phosphorylation	VEGQDMLYQSLKLTN CCCHHHHHHHHCCCC	6.87	27259358
899	Phosphorylation	GQDMLYQSLKLTNGI CHHHHHHHHCCCCCE	17.57	-
919 (in isoform 3)	Ubiquitination	-	36.37	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of AP1B1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of AP1B1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AP1B1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AP1G1_HUMAN	AP1G1	physical	9733768
ARF1_HUMAN	ARF1	physical	11926829
AMPH_HUMAN	AMPH	physical	9280305
KI13A_HUMAN	KIF13A	physical	11106728
AP2M1_HUMAN	AP2M1	physical	7593184
AP1M1_HUMAN	AP1M1	physical	7593184
AP1G1_HUMAN	AP1G1	physical	22939629
AP1M1_HUMAN	AP1M1	physical	22939629
AP1S2_HUMAN	AP1S2	physical	22939629
AP1S1_HUMAN	AP1S1	physical	22939629
NTRK2_HUMAN	NTRK2	physical	21988832
INT9_HUMAN	INTS9	physical	22863883
TBD2A_HUMAN	TBC1D2	physical	22863883
CIP4_HUMAN	TRIP10	physical	22863883
TRM61_HUMAN	TRMT61A	physical	22863883
AP1S1_HUMAN	AP1S1	physical	26344197
AP1S2_HUMAN	AP1S2	physical	26344197
RHG01_HUMAN	ARHGAP1	physical	26344197
FLNB_HUMAN	FLNB	physical	26344197
AAKB1_HUMAN	PRKAB1	physical	26344197
TTC37_HUMAN	TTC37	physical	26344197
UBA1_HUMAN	UBA1	physical	26344197

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Lysine acetylation targets protein complexes and co-regulates majorcellular functions.";
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.;
Science 325:834-840(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-318, AND MASS SPECTROMETRY.
PubMed: 19608861

Phosphorylation

"Global survey of phosphotyrosine signaling identifies oncogenickinases in lung cancer.";
Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J.,Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L.,Mitchell J., Wetzel R., Macneill J., Ren J.M., Yuan J.,Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X.,Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.;
Cell 131:1190-1203(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-897, AND MASSSPECTROMETRY.
PubMed: 18083107