dbPTM

AMPH_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	AMPH_HUMAN
UniProt AC	P49418
Protein Name	Amphiphysin
Gene Name	AMPH
Organism	Homo sapiens (Human).
Sequence Length	695
Subcellular Localization	Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane Peripheral membrane protein Cytoplasmic side. Cytoplasm, cytoskeleton.
Protein Description	May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton..
Protein Sequence	MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGMQEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSRKLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSRRVGFYVNTFKNVSSLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFTIQGAPSDSGPLRIAKTPSPPEEPSPLPSPTASPNHTLAPASPAPARPRSPSQTRKGPPVPPLPKVTPTKELQQENIISFFEDNFVPEISVTTPSQNEVPEVKKEETLLDLDFDPFKPEVTPAGSAGVTHSPMSQTLPWDLWTTSTDLVQPASGGSFNGFTQPQDTSLFTMQTDQSMICNLAESEQAPPTEPKAEEPLAAVTPAVGLDLGMDTRAEEPVEEAVIIPGADADAAVGTLVSAAEGAPGEEAEAEKATVPAGEGVSLEEAKIGTETTEGAESAQPEAEELEATVPQEKVIPSVVIEPASNHEEEGENEITIGAEPKETTEDAAPPGPTSETPELATEQKPIQDPQPTPSAPAMGAADQLASAREASQELPPGFLYKVETLHDFEAANSDELTLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Acetylation	---MADIKTGIFAKN ---CCCHHHHHHHHH	41.04	20167786
5	Ubiquitination	---MADIKTGIFAKN ---CCCHHHHHHHHH	41.04	23000965
6	Phosphorylation	--MADIKTGIFAKNV --CCCHHHHHHHHHH	35.52	18088087
11	Acetylation	IKTGIFAKNVQKRLN HHHHHHHHHHHHHHH	47.07	90365
11	Ubiquitination	IKTGIFAKNVQKRLN HHHHHHHHHHHHHHH	47.07	23000965
15	Acetylation	IFAKNVQKRLNRAQE HHHHHHHHHHHHHHH	55.35	20167786
23	Acetylation	RLNRAQEKVLQKLGK HHHHHHHHHHHHHCC	35.84	72587103
34	Phosphorylation	KLGKADETKDEQFEE HHCCCCCCCHHHHHH	44.32	50565003
63	Phosphorylation	LQRELRGYLAAIKGM HHHHHHHHHHHHHCH	6.17	26846344
74	Phosphorylation	IKGMQEASMKLTESL HHCHHHHHHHHHHHH	18.72	26846344
78	Phosphorylation	QEASMKLTESLHEVY HHHHHHHHHHHHHHH	20.29	26846344
80	Phosphorylation	ASMKLTESLHEVYEP HHHHHHHHHHHHHCC	29.77	30576142
85	Phosphorylation	TESLHEVYEPDWYGR HHHHHHHHCCCCCCH	20.82	26846344
90	Phosphorylation	EVYEPDWYGREDVKM HHHCCCCCCHHHHHH	17.43	26846344
141	Ubiquitination	RIAKRSRKLVDYDSA HHHHHHHHHCCHHHH	54.85	-
145	Phosphorylation	RSRKLVDYDSARHHL HHHHHCCHHHHHHHH	12.34	-
147	Phosphorylation	RKLVDYDSARHHLEA HHHCCHHHHHHHHHH	22.45	110745667
158	Phosphorylation	HLEALQSSKRKDESR HHHHHHHCCCCCHHH	24.42	23403867
210	Phosphorylation	VNTFKNVSSLEAKFH EECCCCHHHHHHHHH	38.30	68739755
211	Phosphorylation	NTFKNVSSLEAKFHK ECCCCHHHHHHHHHH	26.41	23403867
228	Phosphorylation	AVLCHKLYEVMTKLG HHHHHHHHHHHHHHC	15.98	27196784
250	Phosphorylation	FTIQGAPSDSGPLRI EEECCCCCCCCCCCE	43.82	19060867
252	Phosphorylation	IQGAPSDSGPLRIAK ECCCCCCCCCCCEEE	46.80	24076635
260	Phosphorylation	GPLRIAKTPSPPEEP CCCCEEECCCCCCCC	21.70	28348404
262	Phosphorylation	LRIAKTPSPPEEPSP CCEEECCCCCCCCCC	59.32	18510355
268	Phosphorylation	PSPPEEPSPLPSPTA CCCCCCCCCCCCCCC	42.20	20363803
272	Phosphorylation	EEPSPLPSPTASPNH CCCCCCCCCCCCCCC	42.43	11113134
274	Phosphorylation	PSPLPSPTASPNHTL CCCCCCCCCCCCCCC	44.07	29523821
276	Phosphorylation	PLPSPTASPNHTLAP CCCCCCCCCCCCCCC	28.81	18510355
280	Phosphorylation	PTASPNHTLAPASPA CCCCCCCCCCCCCCC	31.12	29449344
285	Phosphorylation	NHTLAPASPAPARPR CCCCCCCCCCCCCCC	22.12	17419807
293	Phosphorylation	PAPARPRSPSQTRKG CCCCCCCCCCCCCCC	31.42	16733250
295	Phosphorylation	PARPRSPSQTRKGPP CCCCCCCCCCCCCCC	45.49	85847
297	Phosphorylation	RPRSPSQTRKGPPVP CCCCCCCCCCCCCCC	38.39	27251275
310	Phosphorylation	VPPLPKVTPTKELQQ CCCCCCCCCCHHHHH	31.22	28978645
312	Phosphorylation	PLPKVTPTKELQQEN CCCCCCCCHHHHHHC	27.99	16733250
333	Phosphorylation	DNFVPEISVTTPSQN CCCCCEEEEECCCCC	16.26	27251275
335	Phosphorylation	FVPEISVTTPSQNEV CCCEEEEECCCCCCC	26.63	27251275
336	Phosphorylation	VPEISVTTPSQNEVP CCEEEEECCCCCCCC	20.84	27251275
350	Phosphorylation	PEVKKEETLLDLDFD CCCCCCCEECCCCCC	33.44	16945112
387	Phosphorylation	LPWDLWTTSTDLVQP CCCCCCCCCCCCEEC	20.32	16945112
445	Phosphorylation	EEPLAAVTPAVGLDL CCCCCCCCHHHCCCC	10.78	21586621
456	Phosphorylation	GLDLGMDTRAEEPVE CCCCCCCCCCCCCCC	23.92	21586631
498	Phosphorylation	EAEAEKATVPAGEGV HHHHHCCCCCCCCCC	37.78	30266825
506	Phosphorylation	VPAGEGVSLEEAKIG CCCCCCCCHHHCCCC	40.70	15345747
507	Phosphorylation	PAGEGVSLEEAKIGT CCCCCCCHHHCCCCC	6.83	32142685
522	Phosphorylation	ETTEGAESAQPEAEE CCCCCHHHCCCCHHH	31.50	-
542	Phosphorylation	PQEKVIPSVVIEPAS CHHHCCCEEEEECCC	20.21	30266825
549	Phosphorylation	SVVIEPASNHEEEGE EEEEECCCCCCCCCC	49.81	18510355
560	Phosphorylation	EEGENEITIGAEPKE CCCCCEEECCCCCCC	13.74	30266825
638	Phosphorylation	HDFEAANSDELTLQR HHHHHCCCCCCEECC	27.95	-
641	Ubiquitination	EAANSDELTLQRGDV HHCCCCCCEECCCCE	7.51	23000965
654	Phosphorylation	DVVLVVPSDSEADQD CEEEEECCCCHHHCC	42.00	27251275
656	Phosphorylation	VLVVPSDSEADQDAG EEEECCCCHHHCCCC	38.74	27251275
683	Ubiquitination	YRDLATYKGLFPENF HHHHHHHCCCCCCCC	44.79	23000965
807	Ubiquitination	----------------------------------------------------------------------------------------------------------------------- -----------------------------------------------------------------------------------------------------------------------		23000965
849	Ubiquitination	----------------------------------------------------------------------------------------------------------------------------------------------------------------- -----------------------------------------------------------------------------------------------------------------------------------------------------------------		23000965
909	Ubiquitination	----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		23000965
1213	Ubiquitination	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		23000965
1255	Ubiquitination	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		23000965

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
272	S	Phosphorylation	Kinase	CDK1	P06493	PSP
272	S	Phosphorylation	Kinase	CDK5	Q00535	PSP
272	S	Phosphorylation	Kinase	CDK_GROUP	-	PhosphoELM
272	S	Phosphorylation	Kinase	CDK-FAMILY	-	GPS
276	S	Phosphorylation	Kinase	CDK1	P06493	PSP
276	S	Phosphorylation	Kinase	CDK5	Q00535	PSP
276	S	Phosphorylation	Kinase	CDK_GROUP	-	PhosphoELM
276	S	Phosphorylation	Kinase	CDK-FAMILY	-	GPS
285	S	Phosphorylation	Kinase	CDK_GROUP	-	PhosphoELM
285	S	Phosphorylation	Kinase	CDK1	P06493	PSP
285	S	Phosphorylation	Kinase	CDK-FAMILY	-	GPS
285	S	Phosphorylation	Kinase	CDK5	Q00535	PSP
293	S	Phosphorylation	Kinase	DYR1A	Q13627	PhosphoELM
295	S	Phosphorylation	Kinase	DYRK1A	Q13627	PSP
310	T	Phosphorylation	Kinase	DYRK1A	Q13627	PSP
312	T	Phosphorylation	Kinase	DYRK1A	Q13627	PSP
350	T	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
387	T	Phosphorylation	Kinase	CSNK2A1	P68400	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of AMPH_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of AMPH_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PLD2_HUMAN	PLD2	physical	10764771
PLD1_HUMAN	PLD1	physical	10764771
DYN1_HUMAN	DNM1	physical	9341169
SH3G2_HUMAN	SH3GL2	physical	9341169
SYNJ1_HUMAN	SYNJ1	physical	9341169
VTNC_HUMAN	VTN	physical	12565847
CLH1_HUMAN	CLTC	physical	9603201
BIN1_HUMAN	BIN1	physical	9348539
DYN1_HUMAN	DNM1	physical	9348539
CD5R1_HUMAN	CDK5R1	physical	11113134
DYN1_HUMAN	DNM1	physical	9280305
CABIN_HUMAN	CABIN1	physical	10931822
DYN1_HUMAN	DNM1	physical	9148966
ITSN1_HUMAN	ITSN1	physical	21712076
REPS1_HUMAN	REPS1	physical	20946875
SGIP1_HUMAN	SGIP1	physical	20946875
REPS2_HUMAN	REPS2	physical	18647389
P73_HUMAN	TP73	physical	11709703
AP2B1_HUMAN	AP2B1	physical	10748223
SYNEM_HUMAN	SYNM	physical	10748223
SYNEM_HUMAN	SYNM	physical	8552632
PP2BC_HUMAN	PPP3CC	physical	25416956
DEUP1_HUMAN	CCDC67	physical	25416956
ARL16_HUMAN	ARL16	physical	25416956
DYN1_RAT	Dnm1	physical	9736607
AP1G1_HUMAN	AP1G1	physical	14973137
BIN1_HUMAN	BIN1	physical	28514442
AP2A2_HUMAN	AP2A2	physical	28514442
AP2A1_HUMAN	AP2A1	physical	28514442
AP1B1_HUMAN	AP1B1	physical	28514442
AP2M1_HUMAN	AP2M1	physical	28514442
AP2S1_HUMAN	AP2S1	physical	28514442
AP2B1_HUMAN	AP2B1	physical	28514442
AAGAB_HUMAN	AAGAB	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of AMPH_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Phosphoproteome of resting human platelets."; Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J.,Schuetz C., Walter U., Gambaryan S., Sickmann A.; J. Proteome Res. 7:526-534(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-6, AND MASSSPECTROMETRY.	18088087 [Abstract]