M4K4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: M4K4_HUMAN
• UniProt AC: O95819
• Protein Name: Mitogen-activated protein kinase kinase kinase kinase 4
• Gene Name: MAP4K4
• Organism: Homo sapiens (Human).
• Sequence Length: 1239
• Subcellular Localization: Cytoplasm .
• Protein Description: Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322..
• Protein Sequence: MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MANDSPAKS ------CCCCCCCHH	39.58	19369195
5	Phosphorylation	---MANDSPAKSLVD ---CCCCCCCHHHEE	26.94	19664994
9	Phosphorylation	ANDSPAKSLVDIDLS CCCCCCHHHEEECHH	35.41	27732954
16	Phosphorylation	SLVDIDLSSLRDPAG HHEEECHHHCCCCCC	24.47	30301811
17	Phosphorylation	LVDIDLSSLRDPAGI HEEECHHHCCCCCCE	34.42	25954137
35	Phosphorylation	VEVVGNGTYGQVYKG EEEECCCCCCEEECC	28.60	18452278
36	Phosphorylation	EVVGNGTYGQVYKGR EEECCCCCCEEECCC	13.63	25884760
40	Phosphorylation	NGTYGQVYKGRHVKT CCCCCEEECCCCCCC	10.39	18452278
46	Ubiquitination	VYKGRHVKTGQLAAI EECCCCCCCCCEEEE	40.59	-
54	Ubiquitination	TGQLAAIKVMDVTED CCCEEEEEEEECCCC	27.12	-
59	Phosphorylation	AIKVMDVTEDEEEEI EEEEEECCCCCHHHH	33.19	25159151
84	Phosphorylation	SHHRNIATYYGAFIK CCCCCHHHHEEEEEC	17.30	24076635
85	Phosphorylation	HHRNIATYYGAFIKK CCCCHHHHEEEEECC	7.29	29083192
86	Phosphorylation	HRNIATYYGAFIKKS CCCHHHHEEEEECCC	9.53	25839225
93	Phosphorylation	YGAFIKKSPPGHDDQ EEEEECCCCCCCHHH	31.18	20860994
124	Phosphorylation	VKNTKGNTLKEDWIA HHCCCCCCCCHHHHH	48.12	24719451
132	Phosphorylation	LKEDWIAYISREILR CCHHHHHHHHHHHHH	7.12	24719451
134	Phosphorylation	EDWIAYISREILRGL HHHHHHHHHHHHHHH	15.93	24719451
155	Ubiquitination	HVIHRDIKGQNVLLT HHCCCCCCCCEEEEC	59.75	-
175	Phosphorylation	KLVDFGVSAQLDRTV EEEEECCEEECCCCC	15.63	30576142
181	Phosphorylation	VSAQLDRTVGRRNTF CEEECCCCCCCCCCC	27.29	30266825
187	Phosphorylation	RTVGRRNTFIGTPYW CCCCCCCCCCCCCCC	18.15	22322096
191	Phosphorylation	RRNTFIGTPYWMAPE CCCCCCCCCCCCCCC	14.37	28464451
193	Phosphorylation	NTFIGTPYWMAPEVI CCCCCCCCCCCCCEE	13.98	28450419
261	Acetylation	KSKKWSKKFFSFIEG CCCHHHHHHHHHHHH	46.24	25953088
261	Ubiquitination	KSKKWSKKFFSFIEG CCCHHHHHHHHHHHH	46.24	-
264	Phosphorylation	KWSKKFFSFIEGCLV HHHHHHHHHHHHHHH	28.57	22322096
279	Phosphorylation	KNYMQRPSTEQLLKH HCCCCCCCHHHHHCC	46.92	21406692
280	Phosphorylation	NYMQRPSTEQLLKHP CCCCCCCHHHHHCCC	30.36	21406692
285	Acetylation	PSTEQLLKHPFIRDQ CCHHHHHCCCCCCCC	58.80	25953088
285	Ubiquitination	PSTEQLLKHPFIRDQ CCHHHHHCCCCCCCC	58.80	2189047
285 (in isoform 1)	Ubiquitination	-	58.80	21890473
285 (in isoform 2)	Ubiquitination	-	58.80	21890473
285 (in isoform 3)	Ubiquitination	-	58.80	21890473
285 (in isoform 4)	Ubiquitination	-	58.80	21890473
285 (in isoform 5)	Ubiquitination	-	58.80	21890473
303	Ubiquitination	RQVRIQLKDHIDRTR CEEHHHHHHHHHHHH	30.77	-
319	Phosphorylation	KRGEKDETEYEYSGS HCCCCCCCCCCCCCC	54.74	22617229
321	Phosphorylation	GEKDETEYEYSGSEE CCCCCCCCCCCCCHH	27.82	22115753
323	Phosphorylation	KDETEYEYSGSEEEE CCCCCCCCCCCHHHH	19.89	22115753
324	Phosphorylation	DETEYEYSGSEEEEE CCCCCCCCCCHHHHC	24.73	22617229
326	Phosphorylation	TEYEYSGSEEEEEEV CCCCCCCCHHHHCCC	35.57	22115753
341	Phosphorylation	PEQEGEPSSIVNVPG CCCCCCCCCCEECCC	28.52	21406692
342	Phosphorylation	EQEGEPSSIVNVPGE CCCCCCCCCEECCCC	41.07	21406692
350	Phosphorylation	IVNVPGESTLRRDFL CEECCCCCHHHHHHH	38.36	18691976
351	Phosphorylation	VNVPGESTLRRDFLR EECCCCCHHHHHHHH	21.71	18691976
367	Phosphorylation	QQENKERSEALRRQQ HHHHHHHHHHHHHHH	28.45	27470641
467	Phosphorylation	RVEREQEYIRRQLEE HHHHHHHHHHHHHHH	10.22	-
519	Phosphorylation	RQLQQEQAYLLSLQH HHHHHHHHHHHHCCC	9.01	27251275
520	Phosphorylation	QLQQEQAYLLSLQHD HHHHHHHHHHHCCCC	14.10	27732954
523	Phosphorylation	QEQAYLLSLQHDHRR HHHHHHHHCCCCCCC	24.32	22322096
536	Phosphorylation	RRPHPQHSQQPPPPQ CCCCCCCCCCCCCCC	25.84	26091039
541 (in isoform 5)	Phosphorylation	-	46.12	21406692
542 (in isoform 4)	Phosphorylation	-	55.02	18691976
543 (in isoform 4)	Phosphorylation	-	64.64	19651622
544 (in isoform 4)	Phosphorylation	-	54.57	18691976
545 (in isoform 5)	Phosphorylation	-	54.39	21406692
546	Methylation	PPPPQQERSKPSFHA CCCCCCCCCCCCCCC	45.72	115478479
546 (in isoform 4)	Phosphorylation	-	45.72	27762562
547	Phosphorylation	PPPQQERSKPSFHAP CCCCCCCCCCCCCCC	48.44	26699800
548	Ubiquitination	PPQQERSKPSFHAPE CCCCCCCCCCCCCCC	51.40	-
550	Phosphorylation	QQERSKPSFHAPEPK CCCCCCCCCCCCCCH	33.14	25159151
550 (in isoform 4)	Phosphorylation	-	33.14	28102081
551 (in isoform 5)	Phosphorylation	-	8.48	20363803
554 (in isoform 5)	Phosphorylation	-	50.26	27732954
556 (in isoform 5)	Phosphorylation	-	37.77	22496350
558 (in isoform 5)	Phosphorylation	-	22.62	25159151
560	Phosphorylation	APEPKAHYEPADRAR CCCCHHCCCHHHHHH	28.59	25884760
560 (in isoform 5)	Phosphorylation	-	28.59	18691976
563 (in isoform 5)	Phosphorylation	-	13.64	25159151
573	Phosphorylation	AREVEDRFRKTNHSS HHHHHHHHHHCCCCC	16.96	27251275
576	Phosphorylation	VEDRFRKTNHSSPEA HHHHHHHCCCCCHHH	33.63	28985074
579	Phosphorylation	RFRKTNHSSPEAQSK HHHHCCCCCHHHHHC	50.05	25849741
580	Phosphorylation	FRKTNHSSPEAQSKQ HHHCCCCCHHHHHCC	21.14	25849741
585	Phosphorylation	HSSPEAQSKQTGRVL CCCHHHHHCCCCCCC	33.50	26074081
588	Phosphorylation	PEAQSKQTGRVLEPP HHHHHCCCCCCCCCC	30.60	26074081
598	Phosphorylation	VLEPPVPSRSESFSN CCCCCCCCCCCCCCC	48.98	22167270
600	Phosphorylation	EPPVPSRSESFSNGN CCCCCCCCCCCCCCC	41.76	19369195
602	Phosphorylation	PVPSRSESFSNGNSE CCCCCCCCCCCCCCC	34.91	25159151
604	Phosphorylation	PSRSESFSNGNSESV CCCCCCCCCCCCCCC	53.78	25850435
608	Phosphorylation	ESFSNGNSESVHPAL CCCCCCCCCCCCHHH	32.42	19664995
608 (in isoform 2)	Phosphorylation	-	32.42	18691976
608 (in isoform 5)	Phosphorylation	-	32.42	28348404
608 (in isoform 6)	Phosphorylation	-	32.42	18691976
610	Phosphorylation	FSNGNSESVHPALQR CCCCCCCCCCHHHCC	26.08	27080861
613	Phosphorylation	GNSESVHPALQRPAE CCCCCCCHHHCCCCC	32.29	27251275
613 (in isoform 2)	Phosphorylation	-	32.29	22199227
613 (in isoform 6)	Phosphorylation	-	32.29	22199227
618 (in isoform 2)	Phosphorylation	-	28.19	22199227
618 (in isoform 6)	Phosphorylation	-	28.19	22199227
619 (in isoform 5)	Phosphorylation	-	43.20	18691976
620 (in isoform 5)	Phosphorylation	-	38.66	19651622
621 (in isoform 5)	Phosphorylation	-	42.44	18691976
623 (in isoform 5)	Phosphorylation	-	7.47	25849741
625	Phosphorylation	PAEPQVPVRTTSRSP CCCCCCCCCCCCCCC	9.98	27251275
625 (in isoform 2)	Phosphorylation	-	9.98	25159151
625 (in isoform 6)	Phosphorylation	-	9.98	25159151
626 (in isoform 2)	Phosphorylation	-	26.64	25159151
626 (in isoform 6)	Phosphorylation	-	26.64	25159151
627	Phosphorylation	EPQVPVRTTSRSPVL CCCCCCCCCCCCCCC	29.38	26846344
627 (in isoform 2)	Phosphorylation	-	29.38	23927012
627 (in isoform 6)	Phosphorylation	-	29.38	23927012
628	Phosphorylation	PQVPVRTTSRSPVLS CCCCCCCCCCCCCCC	16.16	26846344
628 (in isoform 2)	Phosphorylation	-	16.16	23927012
628 (in isoform 6)	Phosphorylation	-	16.16	23927012
629	Phosphorylation	QVPVRTTSRSPVLSR CCCCCCCCCCCCCCC	30.10	26846344
630 (in isoform 3)	Phosphorylation	-	42.77	-
631	Phosphorylation	PVRTTSRSPVLSRRD CCCCCCCCCCCCCCC	21.08	26846344
635	Phosphorylation	TSRSPVLSRRDSPLQ CCCCCCCCCCCCCCC	26.04	23927012
636 (in isoform 3)	Phosphorylation	-	42.11	20363803
639	Phosphorylation	PVLSRRDSPLQGSGQ CCCCCCCCCCCCCCC	26.21	29255136
639 (in isoform 3)	Phosphorylation	-	26.21	27732954
641 (in isoform 3)	Phosphorylation	-	11.88	22496350
643 (in isoform 3)	Phosphorylation	-	41.23	25159151
644	Phosphorylation	RDSPLQGSGQQNSQA CCCCCCCCCCCCCCC	22.07	25159151
645 (in isoform 3)	Phosphorylation	-	39.66	18691976
648 (in isoform 3)	Phosphorylation	-	28.35	25159151
649	Phosphorylation	QGSGQQNSQAGQRNS CCCCCCCCCCCCCCC	19.64	29255136
656	Phosphorylation	SQAGQRNSTSIEPRL CCCCCCCCCCCCHHH	26.22	29255136
657	Phosphorylation	QAGQRNSTSIEPRLL CCCCCCCCCCCHHHH	36.95	29255136
658	Phosphorylation	AGQRNSTSIEPRLLW CCCCCCCCCCHHHHH	25.44	30576142
661 (in isoform 4)	Phosphorylation	-	30.46	28122231
662	Phosphorylation	NSTSIEPRLLWERVE CCCCCCHHHHHHHHH	29.62	27251275
666	Phosphorylation	IEPRLLWERVEKLVP CCHHHHHHHHHHHCC	46.98	27251275
677	Phosphorylation	KLVPRPGSGSSSGSS HHCCCCCCCCCCCCC	37.88	23927012
679	Phosphorylation	VPRPGSGSSSGSSNS CCCCCCCCCCCCCCC	24.03	23927012
680	Phosphorylation	PRPGSGSSSGSSNSG CCCCCCCCCCCCCCC	42.09	23927012
681	Phosphorylation	RPGSGSSSGSSNSGS CCCCCCCCCCCCCCC	44.37	23927012
682	Phosphorylation	PGSGSSSGSSNSGSQ CCCCCCCCCCCCCCC	36.72	27251275
683	Phosphorylation	GSGSSSGSSNSGSQP CCCCCCCCCCCCCCC	28.44	23927012
684	Phosphorylation	SGSSSGSSNSGSQPG CCCCCCCCCCCCCCC	38.24	23401153
685	Phosphorylation	GSSSGSSNSGSQPGS CCCCCCCCCCCCCCC	52.68	27251275
686	Phosphorylation	SSSGSSNSGSQPGSH CCCCCCCCCCCCCCC	41.59	23927012
688	Phosphorylation	SGSSNSGSQPGSHPG CCCCCCCCCCCCCCC	32.78	23927012
692	Phosphorylation	NSGSQPGSHPGSQSG CCCCCCCCCCCCCCC	32.94	23401153
693 (in isoform 3)	Phosphorylation	-	36.33	28348404
696	Phosphorylation	QPGSHPGSQSGSGER CCCCCCCCCCCCCCC	26.14	17525332
698	Phosphorylation	GSHPGSQSGSGERFR CCCCCCCCCCCCCEE	36.30	23927012
700	Phosphorylation	HPGSQSGSGERFRVR CCCCCCCCCCCEEEE	42.60	23927012
704 (in isoform 3)	Phosphorylation	-	8.03	18691976
705 (in isoform 3)	Phosphorylation	-	29.21	19651622
706 (in isoform 3)	Phosphorylation	-	6.82	18691976
708	Phosphorylation	GERFRVRSSSKSEGS CCCEEEECCCCCCCC	35.79	28176443
708 (in isoform 3)	Phosphorylation	-	35.79	25849741
709	Phosphorylation	ERFRVRSSSKSEGSP CCEEEECCCCCCCCH	31.08	28176443
710	Phosphorylation	RFRVRSSSKSEGSPS CEEEECCCCCCCCHH	41.25	28176443
712	Phosphorylation	RVRSSSKSEGSPSQR EEECCCCCCCCHHHH	49.49	23927012
715	Phosphorylation	SSSKSEGSPSQRLEN CCCCCCCCHHHHHHH	19.82	23401153
716 (in isoform 2)	Phosphorylation	-	45.39	28122231
716 (in isoform 3)	Phosphorylation	-	45.39	18691976
717	Phosphorylation	SKSEGSPSQRLENAV CCCCCCHHHHHHHHH	29.63	23927012
721 (in isoform 3)	Phosphorylation	-	45.38	22199227
726	Acetylation	RLENAVKKPEDKKEV HHHHHHCCCCHHHHH	47.22	25953088
726 (in isoform 3)	Phosphorylation	-	47.22	22199227
733 (in isoform 3)	Phosphorylation	-	7.28	25159151
734 (in isoform 3)	Phosphorylation	-	7.55	25159151
735 (in isoform 3)	Phosphorylation	-	40.60	23927012
736 (in isoform 3)	Phosphorylation	-	33.02	23927012
738	Ubiquitination	KEVFRPLKPADLTAL HHHCCCCCHHHHHHH	40.62	-
738 (in isoform 5)	Phosphorylation	-	40.62	28122231
741	Phosphorylation	FRPLKPADLTALAKE CCCCCHHHHHHHHHH	53.75	27251275
743	Phosphorylation	PLKPADLTALAKELR CCCHHHHHHHHHHHH	21.85	30576142
762	Phosphorylation	VRPPHKVTDYSSSSE CCCCCCCCCCCCCCC	33.74	30377224
764	Phosphorylation	PPHKVTDYSSSSEES CCCCCCCCCCCCCCC	10.90	30377224
765	Phosphorylation	PHKVTDYSSSSEESG CCCCCCCCCCCCCCC	26.93	30377224
766	Phosphorylation	HKVTDYSSSSEESGT CCCCCCCCCCCCCCC	31.49	30377224
767	Phosphorylation	KVTDYSSSSEESGTT CCCCCCCCCCCCCCC	35.51	30377224
768	Phosphorylation	VTDYSSSSEESGTTD CCCCCCCCCCCCCCC	47.53	30377224
770	Phosphorylation	DYSSSSEESGTTDEE CCCCCCCCCCCCCCC	57.31	27251275
771	Phosphorylation	YSSSSEESGTTDEED CCCCCCCCCCCCCCC	37.07	18691976
773	Phosphorylation	SSSEESGTTDEEDDD CCCCCCCCCCCCCCH	40.11	30377224
774	Phosphorylation	SSEESGTTDEEDDDV CCCCCCCCCCCCCHH	44.96	30377224
775	Phosphorylation	SEESGTTDEEDDDVE CCCCCCCCCCCCHHH	58.06	27251275
781	Phosphorylation	TDEEDDDVEQEGADE CCCCCCHHHHCCCCC	12.00	27251275
789	Phosphorylation	EQEGADESTSGPEDT HHCCCCCCCCCCHHH	28.55	26074081
790	Phosphorylation	QEGADESTSGPEDTR HCCCCCCCCCCHHHH	35.34	26074081
791	Phosphorylation	EGADESTSGPEDTRA CCCCCCCCCCHHHHC	62.72	26074081
793	Phosphorylation	ADESTSGPEDTRAAS CCCCCCCCHHHHCHH	35.74	27251275
796	Phosphorylation	STSGPEDTRAASSLN CCCCCHHHHCHHHCC	22.12	26074081
798	Phosphorylation	SGPEDTRAASSLNLS CCCHHHHCHHHCCCC	17.49	25627689
798	Phosphorylation	SGPEDTRAASSLNLS CCCHHHHCHHHCCCC	17.49	25627689
798 (in isoform 6)	Phosphorylation	-	17.49	25159151
800	Phosphorylation	PEDTRAASSLNLSNG CHHHHCHHHCCCCCC	34.08	25627689
800	Phosphorylation	PEDTRAASSLNLSNG CHHHHCHHHCCCCCC	34.08	23401153
800 (in isoform 6)	Phosphorylation	-	34.08	25849741
801	Phosphorylation	EDTRAASSLNLSNGE HHHHCHHHCCCCCCC	19.29	30266825
804	Phosphorylation	RAASSLNLSNGETES HCHHHCCCCCCCCCC	5.22	-
804	Phosphorylation	RAASSLNLSNGETES HCHHHCCCCCCCCCC	5.22	27251275
804 (in isoform 6)	Phosphorylation	-	5.22	-
805	Phosphorylation	AASSLNLSNGETESV CHHHCCCCCCCCCCE	41.54	19664994
809	Phosphorylation	LNLSNGETESVKTMI CCCCCCCCCCEEEEE	34.65	30266825
810	Phosphorylation	NLSNGETESVKTMIV CCCCCCCCCEEEEEE	49.59	-
810	Phosphorylation	NLSNGETESVKTMIV CCCCCCCCCEEEEEE	49.59	-
810 (in isoform 6)	Phosphorylation	-	49.59	27282143
811	Phosphorylation	LSNGETESVKTMIVH CCCCCCCCEEEEEEE	36.01	-
811	Phosphorylation	LSNGETESVKTMIVH CCCCCCCCEEEEEEE	36.01	30266825
811 (in isoform 6)	Phosphorylation	-	36.01	27282143
813	Acetylation	NGETESVKTMIVHDD CCCCCCEEEEEEECC	41.47	25953088
814	Phosphorylation	GETESVKTMIVHDDV CCCCCEEEEEEECCC	15.24	24706070
815	Sulfoxidation	ETESVKTMIVHDDVE CCCCEEEEEEECCCC	2.20	21406390
818	Phosphorylation	SVKTMIVHDDVESEP CEEEEEEECCCCCCC	18.03	25850435
818	Phosphorylation	SVKTMIVHDDVESEP CEEEEEEECCCCCCC	18.03	24719451
818 (in isoform 6)	Phosphorylation	-	18.03	25850435
822	Phosphorylation	MIVHDDVESEPAMTP EEEECCCCCCCCCCC	57.27	24719451
822 (in isoform 6)	Phosphorylation	-	57.27	25850435
823	Phosphorylation	IVHDDVESEPAMTPS EEECCCCCCCCCCCC	49.05	30266825
824 (in isoform 3)	Phosphorylation	-	24.47	28122231
828	Phosphorylation	VESEPAMTPSKEGTL CCCCCCCCCCCCCEE	26.90	30266825
830	Phosphorylation	SEPAMTPSKEGTLIV CCCCCCCCCCCEEEE	33.89	23401153
831	Ubiquitination	EPAMTPSKEGTLIVR CCCCCCCCCCEEEEE	62.46	-
834	Phosphorylation	MTPSKEGTLIVRQTQ CCCCCCCEEEEEECC	17.88	30266825
840	O-linked_Glycosylation	GTLIVRQTQSASSTL CEEEEEECCCCCHHH	17.58	28411811
840	Phosphorylation	GTLIVRQTQSASSTL CEEEEEECCCCCHHH	17.58	23927012
842	Phosphorylation	LIVRQTQSASSTLQK EEEEECCCCCHHHHH	33.24	29255136
844	Phosphorylation	VRQTQSASSTLQKHK EEECCCCCHHHHHCC	29.15	23927012
845	Phosphorylation	RQTQSASSTLQKHKS EECCCCCHHHHHCCC	32.62	23927012
846	Phosphorylation	QTQSASSTLQKHKSS ECCCCCHHHHHCCCC	30.61	25159151
849	Methylation	SASSTLQKHKSSSSF CCCHHHHHCCCCCCC	56.99	82996903
849	Ubiquitination	SASSTLQKHKSSSSF CCCHHHHHCCCCCCC	56.99	-
849 (in isoform 6)	Phosphorylation	-	56.99	28348404
850 (in isoform 6)	Phosphorylation	-	23.44	28348404
851	Methylation	SSTLQKHKSSSSFTP CHHHHHCCCCCCCCC	60.10	115970615
851	Ubiquitination	SSTLQKHKSSSSFTP CHHHHHCCCCCCCCC	60.10	-
852	Phosphorylation	STLQKHKSSSSFTPF HHHHHCCCCCCCCCC	34.86	25159151
852 (in isoform 6)	Phosphorylation	-	34.86	28348404
853	Phosphorylation	TLQKHKSSSSFTPFI HHHHCCCCCCCCCCC	34.50	25159151
853 (in isoform 6)	Phosphorylation	-	34.50	28348404
854	Phosphorylation	LQKHKSSSSFTPFID HHHCCCCCCCCCCCC	36.24	23401153
854 (in isoform 6)	Phosphorylation	-	36.24	28348404
855	Phosphorylation	QKHKSSSSFTPFIDP HHCCCCCCCCCCCCH	34.78	25159151
857	Phosphorylation	HKSSSSFTPFIDPRL CCCCCCCCCCCCHHH	21.16	21712546
857 (in isoform 6)	Phosphorylation	-	21.16	28348404
868	Phosphorylation	DPRLLQISPSSGTTV CHHHEEECCCCCCEE	13.03	25159151
870	Phosphorylation	RLLQISPSSGTTVTS HHEEECCCCCCEEEE	34.31	30576142
870 (in isoform 6)	Phosphorylation	-	34.31	22210691
871	Phosphorylation	LLQISPSSGTTVTSV HEEECCCCCCEEEEE	43.47	30576142
873	Phosphorylation	QISPSSGTTVTSVVG EECCCCCCEEEEEEE	21.75	30576142
874	Phosphorylation	ISPSSGTTVTSVVGF ECCCCCCEEEEEEEE	25.88	30576142
874 (in isoform 6)	Phosphorylation	-	25.88	25850435
876	Phosphorylation	PSSGTTVTSVVGFSC CCCCCEEEEEEEEEC	17.45	25627689
876	Phosphorylation	PSSGTTVTSVVGFSC CCCCCEEEEEEEEEC	17.45	30576142
876 (in isoform 6)	Phosphorylation	-	17.45	25159151
877	Phosphorylation	SSGTTVTSVVGFSCD CCCCEEEEEEEEECC	15.52	30576142
878	Phosphorylation	SGTTVTSVVGFSCDG CCCEEEEEEEEECCC	3.40	25627689
878	Phosphorylation	SGTTVTSVVGFSCDG CCCEEEEEEEEECCC	3.40	25627689
878 (in isoform 6)	Phosphorylation	-	3.40	25850435
879	Phosphorylation	GTTVTSVVGFSCDGM CCEEEEEEEEECCCC	6.84	25627689
879	Phosphorylation	GTTVTSVVGFSCDGM CCEEEEEEEEECCCC	6.84	25627689
879 (in isoform 6)	Phosphorylation	-	6.84	25159151
880	Phosphorylation	TTVTSVVGFSCDGMR CEEEEEEEEECCCCC	13.44	25627689
880	Phosphorylation	TTVTSVVGFSCDGMR CEEEEEEEEECCCCC	13.44	25627689
880 (in isoform 6)	Phosphorylation	-	13.44	25159151
882	Phosphorylation	VTSVVGFSCDGMRPE EEEEEEEECCCCCHH	12.84	15345747
886	Phosphorylation	VGFSCDGMRPEAIRQ EEEECCCCCHHHHHC	4.05	25627689
886	Phosphorylation	VGFSCDGMRPEAIRQ EEEECCCCCHHHHHC	4.05	15302935
886 (in isoform 6)	Phosphorylation	-	4.05	29116813
887	Phosphorylation	GFSCDGMRPEAIRQD EEECCCCCHHHHHCC	31.28	25627689
887	Phosphorylation	GFSCDGMRPEAIRQD EEECCCCCHHHHHCC	31.28	25627689
887 (in isoform 6)	Phosphorylation	-	31.28	29116813
888	Phosphorylation	FSCDGMRPEAIRQDP EECCCCCHHHHHCCC	26.86	25627689
888	Phosphorylation	FSCDGMRPEAIRQDP EECCCCCHHHHHCCC	26.86	25627689
888 (in isoform 6)	Phosphorylation	-	26.86	29116813
889	Phosphorylation	SCDGMRPEAIRQDPT ECCCCCHHHHHCCCC	46.49	27251275
891	Phosphorylation	DGMRPEAIRQDPTRK CCCCHHHHHCCCCCC	3.76	27251275
896	Phosphorylation	EAIRQDPTRKGSVVN HHHHCCCCCCCCEEE	53.99	26074081
898	Ubiquitination	IRQDPTRKGSVVNVN HHCCCCCCCCEEECC	59.00	-
900	Phosphorylation	QDPTRKGSVVNVNPT CCCCCCCCEEECCCC	26.40	29255136
902	Phosphorylation	PTRKGSVVNVNPTNT CCCCCCEEECCCCCC	7.68	27251275
907	Phosphorylation	SVVNVNPTNTRPQSD CEEECCCCCCCCCCC	43.98	23927012
909	Phosphorylation	VNVNPTNTRPQSDTP EECCCCCCCCCCCCH	46.32	25159151
911	Phosphorylation	VNPTNTRPQSDTPEI CCCCCCCCCCCCHHH	35.67	27251275
913	Phosphorylation	PTNTRPQSDTPEIRK CCCCCCCCCCHHHHH	46.24	22322096
915	Phosphorylation	NTRPQSDTPEIRKYK CCCCCCCCHHHHHHH	28.24	22322096
928 (in isoform 4)	Ubiquitination	-	40.87	21890473
931 (in isoform 3)	Ubiquitination	-	2.27	-
934	Phosphorylation	SEILCAALWGVNLLV HHHHHHHHHCCCEEE	1.86	27251275
949	Phosphorylation	GTESGLMLLDRSGQG ECCCCEEEEECCCCC	5.60	27251275
957	Ubiquitination	LDRSGQGKVYPLINR EECCCCCCEECCCCC	30.61	-
983 (in isoform 2)	Ubiquitination	-	34.88	21890473
992	Phosphorylation	KKDKLRVYYLSWLRN CCCHHHHEHHHHHHH	7.70	26074081
993	Phosphorylation	KDKLRVYYLSWLRNK CCHHHHEHHHHHHHH	7.45	26074081
995	Phosphorylation	KLRVYYLSWLRNKIL HHHHEHHHHHHHHHH	14.14	23403867
1005 (in isoform 5)	Ubiquitination	-	29.95	21890473
1010	Ubiquitination	HNDPEVEKKQGWTTV CCCHHHHHHCCCEEH	56.42	-
1010 (in isoform 1)	Ubiquitination	-	56.42	21890473
1015	Phosphorylation	VEKKQGWTTVGDLEG HHHHCCCEEHHCCCC	20.59	23532336
1053	Ubiquitination	EVYAWAPKPYHKFMA EEEEECCCCHHHHHE	50.92	-
1057	Ubiquitination	WAPKPYHKFMAFKSF ECCCCHHHHHEECCH	30.87	-
1063	Phosphorylation	HKFMAFKSFGELVHK HHHHEECCHHHHHCC	31.84	20873877
1070	Acetylation	SFGELVHKPLLVDLT CHHHHHCCCEEEEEE	29.86	25953088
1070	Ubiquitination	SFGELVHKPLLVDLT CHHHHHCCCEEEEEE	29.86	-
1090 (in isoform 3)	Ubiquitination	-	5.48	21890473
1124 (in isoform 4)	Ubiquitination	-	4.23	21890473
1143	Phosphorylation	CYEDEGVYVNTYGRI EEECCCEEEECCCCC	9.99	-
1146	Phosphorylation	DEGVYVNTYGRITKD CCCEEEECCCCCCCC	19.80	-
1152	Ubiquitination	NTYGRITKDVVLQWG ECCCCCCCCCHHHCC	46.27	-
1171 (in isoform 2)	Ubiquitination	-	31.34	21890473
1179	Ubiquitination	QTMGWGEKAIEIRSV CCCCCCCCEEEEEEE	51.21	-
1198	Ubiquitination	LDGVFMHKRAQRLKF CCEEEHHHHHHHHHH	37.33	-
1198 (in isoform 1)	Ubiquitination	-	37.33	21890473
1201 (in isoform 5)	Ubiquitination	-	56.10	21890473
1204	Ubiquitination	HKRAQRLKFLCERND HHHHHHHHHHHHCCC	38.18	-
1212	Acetylation	FLCERNDKVFFASVR HHHHCCCEEEEEEEE	44.48	25953088
1212	Ubiquitination	FLCERNDKVFFASVR HHHHCCCEEEEEEEE	44.48	-
1217	Phosphorylation	NDKVFFASVRSGGSS CCEEEEEEEECCCCC	16.95	29759185
1220	Phosphorylation	VFFASVRSGGSSQVY EEEEEEECCCCCEEE	45.40	29759185
1224	Phosphorylation	SVRSGGSSQVYFMTL EEECCCCCEEEEEEE	27.26	-
1227	Phosphorylation	SGGSSQVYFMTLGRT CCCCCEEEEEEECCC	4.68	25839225
1230	Phosphorylation	SSQVYFMTLGRTSLL CCEEEEEEECCCHHC	19.03	23403867
1234	Phosphorylation	YFMTLGRTSLLSW-- EEEEECCCHHCCC--	23.17	29759185
1235	Phosphorylation	FMTLGRTSLLSW--- EEEECCCHHCCC---	26.42	29759185
1278 (in isoform 3)	Ubiquitination	-		21890473

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of M4K4_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of M4K4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of M4K4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RASA1_HUMAN	RASA1	physical	10669731
NCK1_HUMAN	NCK1	physical	9135144
M3K1_HUMAN	MAP3K1	physical	9135144
GBP3_HUMAN	GBP3	physical	12387898
KIF5A_HUMAN	KIF5A	physical	20936779
KPCE_HUMAN	PRKCE	physical	20936779
SL9A1_HUMAN	SLC9A1	physical	20936779
H2B2E_HUMAN	HIST2H2BE	physical	20936779
KI26B_HUMAN	KIF26B	physical	20936779
NDK7_HUMAN	NME7	physical	21988832
CDK12_HUMAN	CDK12	physical	26344197
NOP2_HUMAN	NOP2	physical	26344197

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions.";
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.;
Sci. Signal. 2:RA46-RA46(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-639; SER-800; SER-805AND SER-900, AND MASS SPECTROMETRY.
PubMed: 19690332

"Large-scale proteomics analysis of the human kinome.";
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.;
Mol. Cell. Proteomics 8:1751-1764(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5; THR-181; THR-187;THR-319; TYR-321; TYR-323; SER-324; SER-326; SER-350; THR-351;SER-600; SER-629; SER-631; SER-639; SER-644; SER-656; THR-657;SER-677; SER-679; SER-680; SER-681; SER-683; SER-684; SER-692;SER-696; SER-708; SER-712; SER-715; SER-800; SER-805; THR-828;SER-830; THR-840; SER-842; SER-844; SER-852; SER-853; SER-855;SER-868; SER-900; THR-915 AND THR-1230, AND MASS SPECTROMETRY.
PubMed: 19369195

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-629; SER-631; SER-639;SER-656; SER-658 AND SER-805, AND MASS SPECTROMETRY.
PubMed: 18669648

"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle.";
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.;
Mol. Cell 31:438-448(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324; SER-326; SER-629;SER-631; SER-639; SER-644; SER-656; SER-712; SER-715; SER-805;THR-828; SER-842; SER-855; THR-857; SER-913 AND THR-915, AND MASSSPECTROMETRY.
PubMed: 18691976

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis.";
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III;
J. Proteome Res. 7:1346-1351(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-639, AND MASSSPECTROMETRY.
PubMed: 18220336

"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage.";
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.;
Science 316:1160-1166(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-692 AND SER-696, ANDMASS SPECTROMETRY.
PubMed: 17525332

"Proteomics analysis of protein kinases by target class-selectiveprefractionation and tandem mass spectrometry.";
Wissing J., Jaensch L., Nimtz M., Dieterich G., Hornberger R.,Keri G., Wehland J., Daub H.;
Mol. Cell. Proteomics 6:537-547(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-181; SER-602; SER-629;SER-631; SER-639; SER-801; SER-805 AND SER-900, AND MASS SPECTROMETRY.
PubMed: 17192257

"Large-scale characterization of HeLa cell nuclear phosphoproteins.";
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-629; SER-631 ANDSER-805, AND MASS SPECTROMETRY.
PubMed: 15302935