dbPTM

RASA1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RASA1_HUMAN
UniProt AC	P20936
Protein Name	Ras GTPase-activating protein 1
Gene Name	RASA1
Organism	Homo sapiens (Human).
Sequence Length	1047
Subcellular Localization	Cytoplasm .
Protein Description	Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1..
Protein Sequence	MMAAEAGSEEGGPVTAGAGGGGAAAGSSAYPAVCRVKIPAALPVAAAPYPGLVETGVAGTLGGGAALGSEFLGAGSVAGALGGAGLTGGGTAAGVAGAAAGVAGAAVAGPSGDMALTKLPTSLLAETLGPGGGFPPLPPPPYLPPLGAGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQMNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQEQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCNLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLRIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLTHLLNILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNTTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAARTLILVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLSRDLAALHEICVAHSDELRTLSNERGAQQHVLKKLLAITELLQQKQNQYTKTNDVR

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MMAAEAGS -------CCCCCCCC	20.15	22223895
5 (in isoform 4)	Phosphorylation	-	43.98	29116813
15	Phosphorylation	SEEGGPVTAGAGGGG CCCCCCCCCCCCCCC	23.62	22210691
27	Phosphorylation	GGGAAAGSSAYPAVC CCCCCCCCCCCCCEE	13.68	22210691
164	Phosphorylation	DSLDGPEYEEEEVAI CCCCCCCCCEEEEEC	31.60	22817900
239	Phosphorylation	IIAMCGDYYIGGRRF HHHHHCCEEECCCCC	5.14	27259358
240	Phosphorylation	IAMCGDYYIGGRRFS HHHHCCEEECCCCCC	9.60	24961811
300	Phosphorylation	VPDTDEISFLKGDMF CCCCCCCCEECCCEE	23.60	24719451
418	Phosphorylation	MGGRYYNSIGDIIDH CCCCCCCCHHHHHHH	16.30	23898821
443	Sulfoxidation	YLKEPVPMQDQEQVL ECCCCCCCCCHHHHH	7.55	30846556
453	Phosphorylation	QEQVLNDTVDGKEIY HHHHHHHCCCHHHHH	21.30	-
460	Phosphorylation	TVDGKEIYNTIRRKT CCCHHHHHHHHHHHC	13.82	1850098
462	Phosphorylation	DGKEIYNTIRRKTKD CHHHHHHHHHHHCHH	10.01	28152594
472	Phosphorylation	RKTKDAFYKNIVKKG HHCHHHHHHHHHHHC	12.59	-
480	Phosphorylation	KNIVKKGYLLKKGKG HHHHHHCCEEECCCC	20.10	29341593
581	Phosphorylation	AFCNLRKSSPGTSNK HHHHHCCCCCCCCHH	35.02	24719451
585	Phosphorylation	LRKSSPGTSNKRLRQ HCCCCCCCCHHHHHH	32.29	24719451
586	Phosphorylation	RKSSPGTSNKRLRQV CCCCCCCCHHHHHHH	46.39	28348404
612	Phosphorylation	KLPVKHFTNPYCNIY CCCCCCCCCCCCCEE	34.70	28152594
615	Phosphorylation	VKHFTNPYCNIYLNS CCCCCCCCCCEEECC	10.53	21082442
619	Phosphorylation	TNPYCNIYLNSVQVA CCCCCCEEECCHHHH	5.95	29341593
622	Phosphorylation	YCNIYLNSVQVAKTH CCCEEECCHHHHHHC	16.01	29341593
705	Phosphorylation	LKGIEPGSLRVRARY CCCCCCCCCEEEEEE	24.58	23403867
750	Phosphorylation	HVCGQDRTLLASILL HHHCCCHHHHHHHHH	34.12	23403867
754	Phosphorylation	QDRTLLASILLRIFL CCHHHHHHHHHHHHH	17.46	23403867
767	Phosphorylation	FLHEKLESLLLCTLN HHHHHHHHHHHCCCC	34.45	21955146
772	Phosphorylation	LESLLLCTLNDREIS HHHHHHCCCCCCEEC	28.68	21955146
779	Phosphorylation	TLNDREISMEDEATT CCCCCEECCHHHHHH	16.03	21955146
780	Sulfoxidation	LNDREISMEDEATTL CCCCEECCHHHHHHH	10.39	30846556
785	Phosphorylation	ISMEDEATTLFRATT ECCHHHHHHHHHHHH	23.22	21955146
786	Phosphorylation	SMEDEATTLFRATTL CCHHHHHHHHHHHHH	30.83	21955146
791	Phosphorylation	ATTLFRATTLASTLM HHHHHHHHHHHHHHH	20.34	21955146
792	Phosphorylation	TTLFRATTLASTLME HHHHHHHHHHHHHHH	21.24	21955146
795	Phosphorylation	FRATTLASTLMEQYM HHHHHHHHHHHHHHH	25.78	21955146
796	Phosphorylation	RATTLASTLMEQYMK HHHHHHHHHHHHHHH	25.23	21955146
801	Phosphorylation	ASTLMEQYMKATATQ HHHHHHHHHHHHHHH	6.30	21955146
817	Phosphorylation	VHHALKDSILKIMES HHHHHHHHHHHHHHH	28.38	24719451
824	Phosphorylation	SILKIMESKQSCELS HHHHHHHHHCCCCCC	20.72	29449344
827	Phosphorylation	KIMESKQSCELSPSK HHHHHHCCCCCCHHH	17.19	23927012
831	Phosphorylation	SKQSCELSPSKLEKN HHCCCCCCHHHHCCC	12.58	30266825
833	Phosphorylation	QSCELSPSKLEKNED CCCCCCHHHHCCCCC	46.63	30266825
834	Malonylation	SCELSPSKLEKNEDV CCCCCHHHHCCCCCC	65.14	26320211
863	Phosphorylation	VEKIFMASEILPPTL HHHHHHHHHCCCHHH	17.24	19845377
874	Phosphorylation	PPTLRYIYGCLQKSV CHHHHHHHHHHHHHH	7.89	19845377
952	Phosphorylation	EFGAKEPYMEGVNPF HHCCCCCHHCCCCHH	14.64	27259358

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
460	Y	Phosphorylation	Kinase	EGFR	P00533	PhosphoELM
460	Y	Phosphorylation	Kinase	SRC	P12931	GPS
460	Y	Phosphorylation	Kinase	PDGFR-FAMILY	-	GPS
460	Y	Phosphorylation	Kinase	PDGFR_GROUP	-	PhosphoELM

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RASA1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RASA1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DOK1_HUMAN	DOK1	physical	10822173
DOK2_HUMAN	DOK2	physical	10822173
PAXI_HUMAN	PXN	physical	10092539
KHDR1_HUMAN	KHDRBS1	physical	11604231
KHDR1_HUMAN	KHDRBS1	physical	9743338
YES_HUMAN	YES1	physical	1544885
NCK1_HUMAN	NCK1	physical	9233798
EPHB2_HUMAN	EPHB2	physical	9233798
HD_HUMAN	HTT	physical	9079622
SRC_HUMAN	SRC	physical	1717825
DOK1_HUMAN	DOK1	physical	10799545
ANXA6_HUMAN	ANXA6	physical	10571081
EPHB3_HUMAN	EPHB3	physical	9674711
EPHB2_HUMAN	EPHB2	physical	10644995
CAV2_HUMAN	CAV2	physical	12091389
G3BP1_HUMAN	G3BP1	physical	9632780
RASH_HUMAN	HRAS	physical	7628625
RASH_HUMAN	HRAS	physical	9219684
PGFRB_HUMAN	PDGFRB	physical	11896619
RASH_HUMAN	HRAS	physical	11389730
IKBA_HUMAN	NFKBIA	genetic	12151397
FBW1A_HUMAN	BTRC	genetic	12151397
NCK1_HUMAN	NCK1	physical	21664272
TRM2A_HUMAN	TRMT2A	physical	21900206
BBS10_HUMAN	BBS10	physical	21900206
ZN579_HUMAN	ZNF579	physical	21900206
KAIN_HUMAN	SERPINA4	physical	21900206
XRCC6_HUMAN	XRCC6	physical	21900206
EIF1_HUMAN	EIF1	physical	21900206
EZH2_HUMAN	EZH2	physical	21900206
SOCS1_HUMAN	SOCS1	physical	18556463
A4_HUMAN	APP	physical	21832049
ERBB3_HUMAN	ERBB3	physical	16273093
ERBB4_HUMAN	ERBB4	physical	16273093
ABL1_HUMAN	ABL1	physical	1571536
PLCG1_HUMAN	PLCG1	physical	8977179
DOK2_HUMAN	DOK2	physical	9478921
DOK1_HUMAN	DOK1	physical	9478921
KHDR1_HUMAN	KHDRBS1	physical	1385407
EGFR_HUMAN	EGFR	physical	1385407
DOK1_MOUSE	Dok1	physical	10202139
RHG35_HUMAN	ARHGAP35	physical	8419471
KHDR1_HUMAN	KHDRBS1	physical	8419471
RHG35_HUMAN	ARHGAP35	physical	8189062
KHDR1_HUMAN	KHDRBS1	physical	8189062
OLIG1_HUMAN	OLIG1	physical	25814554
RASH_HUMAN	HRAS	physical	26721396

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
	Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
608354
608355	Parkes Weber syndrome (PKWS)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RASA1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Global survey of phosphotyrosine signaling identifies oncogenickinases in lung cancer."; Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J.,Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L.,Mitchell J., Wetzel R., Macneill J., Ren J.M., Yuan J.,Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X.,Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.; Cell 131:1190-1203(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-615, AND MASSSPECTROMETRY.	18083107 [Abstract]
"Immunoaffinity profiling of tyrosine phosphorylation in cancercells."; Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H.,Zha X.-M., Polakiewicz R.D., Comb M.J.; Nat. Biotechnol. 23:94-101(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-615, AND MASSSPECTROMETRY.	15592455 [Abstract]