dbPTM

KIBRA_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	KIBRA_HUMAN
UniProt AC	Q8IX03
Protein Name	Protein KIBRA
Gene Name	WWC1
Organism	Homo sapiens (Human).
Sequence Length	1113
Subcellular Localization	Cytoplasm. Cytoplasm, perinuclear region. Nucleus. Cell projection, ruffle membrane. Colocalizes with PRKCZ in the perinuclear region.
Protein Description	Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. Modulates directional migration of podocytes. Acts as a substrate for PRKCZ. Plays a role in cognition and memory performance..
Protein Sequence	MPRPELPLPEGWEEARDFDGKVYYIDHTNRTTSWIDPRDRYTKPLTFADCISDELPLGWEEAYDPQVGDYFIDHNTKTTQIEDPRVQWRREQEHMLKDYLVVAQEALSAQKEIYQVKQQRLELAQQEYQQLHAVWEHKLGSQVSLVSGSSSSSKYDPEILKAEIATAKSRVNKLKREMVHLQHELQFKERGFQTLKKIDKKMSDAQGSYKLDEAQAVLRETKAIKKAITCGEKEKQDLIKSLAMLKDGFRTDRGSHSDLWSSSSSLESSSFPLPKQYLDVSSQTDISGSFGINSNNQLAEKVRLRLRYEEAKRRIANLKIQLAKLDSEAWPGVLDSERDRLILINEKEELLKEMRFISPRKWTQGEVEQLEMARKRLEKDLQAARDTQSKALTERLKLNSKRNQLVRELEEATRQVATLHSQLKSLSSSMQSLSSGSSPGSLTSSRGSLVASSLDSSTSASFTDLYYDPFEQLDSELQSKVEFLLLEGATGFRPSGCITTIHEDEVAKTQKAEGGGRLQALRSLSGTPKSMTSLSPRSSLSSPSPPCSPLMADPLLAGDAFLNSLEFEDPELSATLCELSLGNSAQERYRLEEPGTEGKQLGQAVNTAQGCGLKVACVSAAVSDESVAGDSGVYEASVQRLGASEAAAFDSDESEAVGATRIQIALKYDEKNKQFAILIIQLSNLSALLQQQDQKVNIRVAVLPCSESTTCLFRTRPLDASDTLVFNEVFWVSMSYPALHQKTLRVDVCTTDRSHLEECLGGAQISLAEVCRSGERSTRWYNLLSYKYLKKQSRELKPVGVMAPASGPASTDAVSALLEQTAVELEKRQEGRSSTQTLEDSWRYEETSENEAVAEEEEEEVEEEEGEEDVFTEKASPDMDGYPALKVDKETNTETPAPSPTVVRPKDRRVGTPSQGPFLRGSTIIRSKTFSPGPQSQYVCRLNRSDSDSSTLSKKPPFVRNSLERRSVRMKRPSSVKSLRSERLIRTSLDLELDLQATRTWHSQLTQEISVLKELKEQLEQAKSHGEKELPQWLREDERFRLLLRMLEKRQMDRAEHKGELQTDKMMRAAAKDVHRLRGQSCKEPPEVQSFREKMAFFTRPRMNIPALSADDV

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
24 (in isoform 2)	Phosphorylation	-	11.67	27642862
33	Phosphorylation	DHTNRTTSWIDPRDR ECCCCEECCCCCHHC	22.78	28857561
117	Ubiquitination	QKEIYQVKQQRLELA HHHHHHHHHHHHHHH	25.78	29967540
128	Phosphorylation	LELAQQEYQQLHAVW HHHHHHHHHHHHHHH	9.38	24927040
141	Phosphorylation	VWEHKLGSQVSLVSG HHHHCCCCCEEEEEC	37.53	28985074
141 (in isoform 2)	Phosphorylation	-	37.53	27251275
144	Phosphorylation	HKLGSQVSLVSGSSS HCCCCCEEEEECCCC	18.54	20873877
149	Phosphorylation	QVSLVSGSSSSSKYD CEEEEECCCCCCCCC	21.38	25159151
150	Phosphorylation	VSLVSGSSSSSKYDP EEEEECCCCCCCCCH	37.45	28857561
151	Phosphorylation	SLVSGSSSSSKYDPE EEEECCCCCCCCCHH	40.09	25159151
152	Phosphorylation	LVSGSSSSSKYDPEI EEECCCCCCCCCHHH	32.56	25159151
153	Phosphorylation	VSGSSSSSKYDPEIL EECCCCCCCCCHHHH	36.86	25159151
154	Acetylation	SGSSSSSKYDPEILK ECCCCCCCCCHHHHH	56.47	130419
161	Ubiquitination	KYDPEILKAEIATAK CCCHHHHHHHHHHHH	50.03	29967540
208	Phosphorylation	KMSDAQGSYKLDEAQ HCCCCCCCCCHHHHH	13.65	28857561
208 (in isoform 2)	Phosphorylation	-	13.65	27251275
209	Phosphorylation	MSDAQGSYKLDEAQA CCCCCCCCCHHHHHH	23.53	23312004
229	Phosphorylation	KAIKKAITCGEKEKQ HHHHHHHCCCHHHHH	21.22	29978859
241	Phosphorylation	EKQDLIKSLAMLKDG HHHHHHHHHHHHHHC	17.86	23312004
251	Phosphorylation	MLKDGFRTDRGSHSD HHHHCCCCCCCCHHH	27.99	28450419
255	Phosphorylation	GFRTDRGSHSDLWSS CCCCCCCCHHHCCCC	22.25	28450419
255 (in isoform 2)	Phosphorylation	-	22.25	27251275
257	Phosphorylation	RTDRGSHSDLWSSSS CCCCCCHHHCCCCCC	35.78	28450419
261	Phosphorylation	GSHSDLWSSSSSLES CCHHHCCCCCCCCCC	27.86	20873877
262	Phosphorylation	SHSDLWSSSSSLESS CHHHCCCCCCCCCCC	23.74	30576142
263	Phosphorylation	HSDLWSSSSSLESSS HHHCCCCCCCCCCCC	20.59	28348404
264	Phosphorylation	SDLWSSSSSLESSSF HHCCCCCCCCCCCCC	40.67	28348404
265	Phosphorylation	DLWSSSSSLESSSFP HCCCCCCCCCCCCCC	37.80	28348404
268	Phosphorylation	SSSSSLESSSFPLPK CCCCCCCCCCCCCCH	36.25	28348404
269	Phosphorylation	SSSSLESSSFPLPKQ CCCCCCCCCCCCCHH	26.99	28348404
270	Phosphorylation	SSSLESSSFPLPKQY CCCCCCCCCCCCHHH	39.91	28348404
324	Ubiquitination	NLKIQLAKLDSEAWP HHHHHEEECCCCCCC	62.51	29967540
327	O-linked_Glycosylation	IQLAKLDSEAWPGVL HHEEECCCCCCCCCC	39.38	30379171
347	2-Hydroxyisobutyrylation	RLILINEKEELLKEM EEEECCCHHHHHHHC	52.58	-
347	Acetylation	RLILINEKEELLKEM EEEECCCHHHHHHHC	52.58	21466224
347	Ubiquitination	RLILINEKEELLKEM EEEECCCHHHHHHHC	52.58	33845483
358	Phosphorylation	LKEMRFISPRKWTQG HHHCCCCCCCCCCHH	18.88	22210691
358 (in isoform 2)	Phosphorylation	-	18.88	24719451
379	Ubiquitination	MARKRLEKDLQAARD HHHHHHHHHHHHHHH	69.22	29967540
427	Phosphorylation	HSQLKSLSSSMQSLS HHHHHHHHHHHHHHH	27.71	-
434	Phosphorylation	SSSMQSLSSGSSPGS HHHHHHHHCCCCCCC	37.59	25159151
435	Phosphorylation	SSMQSLSSGSSPGSL HHHHHHHCCCCCCCC	48.24	28348404
437	Phosphorylation	MQSLSSGSSPGSLTS HHHHHCCCCCCCCCC	34.99	25159151
438	Phosphorylation	QSLSSGSSPGSLTSS HHHHCCCCCCCCCCC	36.48	28985074
441	Phosphorylation	SSGSSPGSLTSSRGS HCCCCCCCCCCCCCC	31.68	28348404
443	Phosphorylation	GSSPGSLTSSRGSLV CCCCCCCCCCCCCEE	26.76	28348404
444	Phosphorylation	SSPGSLTSSRGSLVA CCCCCCCCCCCCEEE	24.27	28348404
445	Phosphorylation	SPGSLTSSRGSLVAS CCCCCCCCCCCEEEC	35.11	28348404
523	Phosphorylation	GRLQALRSLSGTPKS CHHHHHHHCCCCCCC	27.95	25159151
523 (in isoform 2)	Phosphorylation	-	27.95	24719451
525	Phosphorylation	LQALRSLSGTPKSMT HHHHHHCCCCCCCCC	41.70	25159151
527	Phosphorylation	ALRSLSGTPKSMTSL HHHHCCCCCCCCCCC	24.94	25159151
527 (in isoform 2)	Phosphorylation	-	24.94	24719451
530	Phosphorylation	SLSGTPKSMTSLSPR HCCCCCCCCCCCCCC	28.61	22777824
532	Phosphorylation	SGTPKSMTSLSPRSS CCCCCCCCCCCCCCC	33.34	25159151
532 (in isoform 2)	Phosphorylation	-	33.34	24719451
533	Phosphorylation	GTPKSMTSLSPRSSL CCCCCCCCCCCCCCC	20.43	25159151
535	Phosphorylation	PKSMTSLSPRSSLSS CCCCCCCCCCCCCCC	20.31	28355574
535 (in isoform 2)	Phosphorylation	-	20.31	27251275
539	Phosphorylation	TSLSPRSSLSSPSPP CCCCCCCCCCCCCCC	33.28	21878642
542	Phosphorylation	SPRSSLSSPSPPCSP CCCCCCCCCCCCCCC	34.39	22784093
548	Phosphorylation	SSPSPPCSPLMADPL CCCCCCCCCCCCCHH	27.17	-
596	Phosphorylation	YRLEEPGTEGKQLGQ HCCCCCCCCHHHHHH	52.89	28555341
607	Phosphorylation	QLGQAVNTAQGCGLK HHHHHHHHHCCCCCE	17.48	25332170
619	Phosphorylation	GLKVACVSAAVSDES CCEEEEEEEEECCCC	15.47	22210691
623	Phosphorylation	ACVSAAVSDESVAGD EEEEEEECCCCCCCC	30.82	28985074
623 (in isoform 2)	Phosphorylation	-	30.82	27251275
626	Phosphorylation	SAAVSDESVAGDSGV EEEECCCCCCCCCCC	23.43	28348404
626 (in isoform 2)	Phosphorylation	-	23.43	27251275
631	Phosphorylation	DESVAGDSGVYEASV CCCCCCCCCCHHHHH	29.28	28348404
634 (in isoform 2)	Phosphorylation	-	14.87	27642862
644	Phosphorylation	SVQRLGASEAAAFDS HHHHHCCCHHCCCCC	26.43	30278072
651	Phosphorylation	SEAAAFDSDESEAVG CHHCCCCCCCHHHHC	36.50	30278072
651 (in isoform 2)	Phosphorylation	-	36.50	24719451
654	Phosphorylation	AAFDSDESEAVGATR CCCCCCCHHHHCCCE	35.53	30278072
660	Phosphorylation	ESEAVGATRIQIALK CHHHHCCCEEEEEEE	23.52	26270265
781	Phosphorylation	GERSTRWYNLLSYKY CCCCHHHHHHHHHHH	7.82	26074081
785	Phosphorylation	TRWYNLLSYKYLKKQ HHHHHHHHHHHHHHC	23.81	26074081
786	Phosphorylation	RWYNLLSYKYLKKQS HHHHHHHHHHHHHCC	12.01	26074081
788	Phosphorylation	YNLLSYKYLKKQSRE HHHHHHHHHHHCCCC	18.60	26074081
793	Phosphorylation	YKYLKKQSRELKPVG HHHHHHCCCCCCCCC	36.28	26074081
815	Phosphorylation	PASTDAVSALLEQTA CCCHHHHHHHHHHHH	18.21	-
833	Phosphorylation	EKRQEGRSSTQTLED HHHHCCCCCCCCHHH	48.90	23663014
834	Phosphorylation	KRQEGRSSTQTLEDS HHHCCCCCCCCHHHH	24.15	23663014
834 (in isoform 2)	Phosphorylation	-	24.15	27251275
835	Phosphorylation	RQEGRSSTQTLEDSW HHCCCCCCCCHHHHH	27.16	23663014
837	Phosphorylation	EGRSSTQTLEDSWRY CCCCCCCCHHHHHHC	32.00	-
841	Phosphorylation	STQTLEDSWRYEETS CCCCHHHHHHCCCCC	12.22	21815630
847	Phosphorylation	DSWRYEETSENEAVA HHHHCCCCCCCCCCC	29.42	28348404
848	Phosphorylation	SWRYEETSENEAVAE HHHCCCCCCCCCCCH	41.52	28348404
876	Phosphorylation	DVFTEKASPDMDGYP CCCCCCCCCCCCCCC	32.28	25159151
876 (in isoform 2)	Phosphorylation	-	32.28	27251275
882	Phosphorylation	ASPDMDGYPALKVDK CCCCCCCCCCEEECC	4.89	27251275
891	Phosphorylation	ALKVDKETNTETPAP CEEECCCCCCCCCCC	53.86	30266825
893	Phosphorylation	KVDKETNTETPAPSP EECCCCCCCCCCCCC	48.97	30266825
895	Phosphorylation	DKETNTETPAPSPTV CCCCCCCCCCCCCCE	23.78	30266825
895 (in isoform 2)	Phosphorylation	-	23.78	27251275
899	Phosphorylation	NTETPAPSPTVVRPK CCCCCCCCCCEECCC	35.00	30266825
899 (in isoform 2)	Phosphorylation	-	35.00	27251275
901	Phosphorylation	ETPAPSPTVVRPKDR CCCCCCCCEECCCCC	35.65	30266825
912	Phosphorylation	PKDRRVGTPSQGPFL CCCCCCCCCCCCCCC	19.53	25159151
912 (in isoform 2)	Phosphorylation	-	19.53	27251275
914	Phosphorylation	DRRVGTPSQGPFLRG CCCCCCCCCCCCCCC	47.83	23186163
922	Phosphorylation	QGPFLRGSTIIRSKT CCCCCCCCEEEEECC	15.25	17924679
923	Phosphorylation	GPFLRGSTIIRSKTF CCCCCCCEEEEECCC	24.21	17924679
927	Phosphorylation	RGSTIIRSKTFSPGP CCCEEEEECCCCCCC	26.16	26657352
927 (in isoform 2)	Phosphorylation	-	26.16	24719451
929	Phosphorylation	STIIRSKTFSPGPQS CEEEEECCCCCCCCH	30.50	25159151
929 (in isoform 2)	Phosphorylation	-	30.50	27251275
931	Phosphorylation	IIRSKTFSPGPQSQY EEEECCCCCCCCHHE	33.89	25159151
936	Phosphorylation	TFSPGPQSQYVCRLN CCCCCCCHHEEEECC	26.98	23186163
938	Phosphorylation	SPGPQSQYVCRLNRS CCCCCHHEEEECCCC	13.53	-
945	Phosphorylation	YVCRLNRSDSDSSTL EEEECCCCCCCCCCC	40.15	25159151
947	Phosphorylation	CRLNRSDSDSSTLSK EECCCCCCCCCCCCC	40.41	28355574
947 (in isoform 2)	Phosphorylation	-	40.41	24719451
949	Phosphorylation	LNRSDSDSSTLSKKP CCCCCCCCCCCCCCC	29.22	28176443
950	Phosphorylation	NRSDSDSSTLSKKPP CCCCCCCCCCCCCCC	38.06	29396449
951	Phosphorylation	RSDSDSSTLSKKPPF CCCCCCCCCCCCCCC	38.72	29396449
953	Phosphorylation	DSDSSTLSKKPPFVR CCCCCCCCCCCCCCC	38.43	29396449
962	Phosphorylation	KPPFVRNSLERRSVR CCCCCCCCHHHCCCC	22.50	23186163
974	Phosphorylation	SVRMKRPSSVKSLRS CCCCCCCHHHHHHHH	52.36	28176443
974 (in isoform 2)	Phosphorylation	-	52.36	30266825
975	Phosphorylation	VRMKRPSSVKSLRSE CCCCCCHHHHHHHHH	35.93	15081397
978	Phosphorylation	KRPSSVKSLRSERLI CCCHHHHHHHHHHHH	27.19	14702039
981	Phosphorylation	SSVKSLRSERLIRTS HHHHHHHHHHHHHHH	32.15	28634120
1006	Phosphorylation	RTWHSQLTQEISVLK HHHHHHHHHHHHHHH	19.27	-
1081	Phosphorylation	VHRLRGQSCKEPPEV HHHHCCCCCCCCCHH	29.94	25159151
1087 (in isoform 2)	Phosphorylation	-	65.00	27251275

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
539	S	Phosphorylation	Kinase	AURKA	O14965	GPS
539	S	Phosphorylation	Kinase	AURKB	Q96GD4	GPS
542	S	Phosphorylation	Kinase	CDK1	P06493	Uniprot
542	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
542	S	Phosphorylation	Kinase	MAPK3	P27361	GPS
548	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
548	S	Phosphorylation	Kinase	MAPK3	P27361	GPS
929	T	Phosphorylation	Kinase	RPS6KA1	Q15418	GPS
929	T	Phosphorylation	Kinase	RPS6KA3	P51812	GPS
931	S	Phosphorylation	Kinase	CDK1	P06493	Uniprot
947	S	Phosphorylation	Kinase	RPS6KA1	Q15418	GPS
947	S	Phosphorylation	Kinase	RPS6KA3	P51812	GPS
975	S	Phosphorylation	Kinase	PKCZ	Q05513	PSP
978	S	Phosphorylation	Kinase	PKCZ	Q05513	PSP
1006	T	Phosphorylation	Kinase	ATM	Q13315	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
542	S	Phosphorylation	22784093
Phosphorylation at Ser-542 and Ser-931 by CDK1 in response to spindle damage stress regulates mitotic exit, these two sites are dephosphorylated by CDC14B.
931	S	Phosphorylation	18669648
Phosphorylation at Ser-542 and Ser-931 by CDK1 in response to spindle damage stress regulates mitotic exit, these two sites are dephosphorylated by CDC14B.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of KIBRA_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KPCZ_HUMAN	PRKCZ	physical	15081397
CDC73_HUMAN	CDC73	physical	16169070
LRIF1_HUMAN	LRIF1	physical	16169070
RHG07_HUMAN	DLC1	physical	16684779
H31_HUMAN	HIST1H3A	physical	16684779
LATS1_HUMAN	LATS1	physical	21233212
LATS2_HUMAN	LATS2	physical	21233212
MERL_HUMAN	NF2	physical	21233212
PTN14_HUMAN	PTPN14	physical	25023289
LATS1_HUMAN	LATS1	physical	25023289
1433B_HUMAN	YWHAB	physical	24366813
1433E_HUMAN	YWHAE	physical	24366813
1433F_HUMAN	YWHAH	physical	24366813
1433G_HUMAN	YWHAG	physical	24366813
1433T_HUMAN	YWHAQ	physical	24366813
AMOT_HUMAN	AMOT	physical	24366813
ATD3A_HUMAN	ATAD3A	physical	24366813
DYL1_HUMAN	DYNLL1	physical	24366813
DYL2_HUMAN	DYNLL2	physical	24366813
E41L2_HUMAN	EPB41L2	physical	24366813
E41L3_HUMAN	EPB41L3	physical	24366813
FBW1A_HUMAN	BTRC	physical	24366813
FBW1B_HUMAN	FBXW11	physical	24366813
PTN14_HUMAN	PTPN14	physical	24366813
WWC2_HUMAN	WWC2	physical	24682284
WWC3_HUMAN	WWC3	physical	24682284
KPCA_HUMAN	PRKCA	physical	24682284
LATS2_HUMAN	LATS2	physical	24682284
LATS1_HUMAN	LATS1	physical	24682284
KPCZ_RAT	Prkcz	physical	15081397
CTRO_HUMAN	CIT	physical	28720576
AMOT_HUMAN	AMOT	physical	28720576
USP9X_HUMAN	USP9X	physical	28720576
MPDZ_HUMAN	MPDZ	physical	28720576
INADL_HUMAN	INADL	physical	28720576
RPGF6_HUMAN	RAPGEF6	physical	28720576
AMOL1_HUMAN	AMOTL1	physical	28720576
MPP5_HUMAN	MPP5	physical	28720576
DLG1_HUMAN	DLG1	physical	28720576
DCTN1_HUMAN	DCTN1	physical	28720576
MAEA_HUMAN	MAEA	physical	28720576
MYO1C_HUMAN	MYO1C	physical	28720576
RMD5A_HUMAN	RMND5A	physical	28720576
KPCI_HUMAN	PRKCI	physical	28720576
ARMC8_HUMAN	ARMC8	physical	28720576
CPVL_HUMAN	CPVL	physical	28720576
LIN7C_HUMAN	LIN7C	physical	28720576
DAG1_HUMAN	DAG1	physical	28720576
PTN14_HUMAN	PTPN14	physical	28720576
KC1E_HUMAN	CSNK1E	physical	28720576

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of KIBRA_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-912; THR-929 ANDSER-931, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Improved titanium dioxide enrichment of phosphopeptides from HeLacells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."; Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.; J. Proteome Res. 6:4150-4162(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-922; THR-923 ANDSER-927, AND MASS SPECTROMETRY.	17924679 [Abstract]
"KIBRA is a novel substrate for protein kinase Czeta."; Buether K., Plaas C., Barnekow A., Kremerskothen J.; Biochem. Biophys. Res. Commun. 317:703-707(2004). Cited for: FUNCTION, SUBUNIT, INTERACTION WITH PRKCZ, AND PHOSPHORYLATION ATSER-975 AND SER-978.	15081397 [Abstract]

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