dbPTM

XRCC3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	XRCC3_HUMAN
UniProt AC	O43542
Protein Name	DNA repair protein XRCC3
Gene Name	XRCC3
Organism	Homo sapiens (Human).
Sequence Length	346
Subcellular Localization	Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
Protein Description	Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD21 paralog protein complex CX3 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, CX3 acts downstream of RAD51 recruitment; the complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex and seems to involve GEN1 during mitotic cell cycle progression. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C..
Protein Sequence	MDLDLLDLNPRIIAAIKKAKLKSVKEVLHFSGPDLKRLTNLSSPEVWHLLRTASLHLRGSSILTALQLHQQKERFPTQHQRLSLGCPVLDALLRGGLPLDGITELAGRSSAGKTQLALQLCLAVQFPRQHGGLEAGAVYICTEDAFPHKRLQQLMAQQPRLRTDVPGELLQKLRFGSQIFIEHVADVDTLLECVNKKVPVLLSRGMARLVVIDSVAAPFRCEFDSQASAPRARHLQSLGATLRELSSAFQSPVLCINQVTEAMEEQGAAHGPLGFWDERVSPALGITWANQLLVRLLADRLREEEAALGCPARTLRVLSAPHLPPSSCSYTISAEGVRGTPGTQSH

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MDLDLLDL -------CCCCHHCC	11.87	22814378
17	2-Hydroxyisobutyrylation	PRIIAAIKKAKLKSV HHHHHHHHHHHCCCH	41.79	-
18	Ubiquitination	RIIAAIKKAKLKSVK HHHHHHHHHHCCCHH	44.64	-
23	Phosphorylation	IKKAKLKSVKEVLHF HHHHHCCCHHHHHHH	49.19	-
25	Ubiquitination	KAKLKSVKEVLHFSG HHHCCCHHHHHHHCC	49.55	-
36	Ubiquitination	HFSGPDLKRLTNLSS HHCCCCHHHHCCCCC	53.38	-
39	Phosphorylation	GPDLKRLTNLSSPEV CCCHHHHCCCCCHHH	37.84	27732954
42	Phosphorylation	LKRLTNLSSPEVWHL HHHHCCCCCHHHHHH	46.01	24719451
43	Phosphorylation	KRLTNLSSPEVWHLL HHHCCCCCHHHHHHH	28.39	27732954
52	Phosphorylation	EVWHLLRTASLHLRG HHHHHHHHHHHHHCC	22.14	24719451
72	Ubiquitination	ALQLHQQKERFPTQH HHHHHHHHHCCCCHH	44.26	-
172	Ubiquitination	VPGELLQKLRFGSQI CCHHHHHHHHHCCEE	41.41	2190698
177	Phosphorylation	LQKLRFGSQIFIEHV HHHHHHCCEEEEEEE	19.86	-
189	Phosphorylation	EHVADVDTLLECVNK EEECCHHHHHHHHHC	32.84	-
196	Ubiquitination	TLLECVNKKVPVLLS HHHHHHHCCCCHHHH	34.45	-
197	Ubiquitination	LLECVNKKVPVLLSR HHHHHHCCCCHHHHC	46.71	-
225	Phosphorylation	PFRCEFDSQASAPRA CEECCCCCCCCCCHH	32.73	17525332
241	Phosphorylation	HLQSLGATLRELSSA HHHHHHHHHHHHHHH	25.26	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
225	S	Phosphorylation	Kinase	ATM	Q13315	PSP
225	S	Phosphorylation	Kinase	ATR	Q13535	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of XRCC3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of XRCC3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RA51C_HUMAN	RAD51C	physical	11331762
ZNHI3_HUMAN	ZNHIT3	physical	20211142
FANCG_HUMAN	FANCG	physical	20450923
FANCG_HUMAN	FANCG	physical	18212739
FANCG_HUMAN	FANCG	physical	16621732
SWAP1_HUMAN	SWSAP1	physical	21965664
BAP1_HUMAN	BAP1	physical	25640309
BCAS3_HUMAN	BCAS3	physical	25640309
GROA_HUMAN	CXCL1	physical	25640309
FA84B_HUMAN	FAM84B	physical	25640309
GREB1_HUMAN	GREB1	physical	25640309
ITIH5_HUMAN	ITIH5	physical	25640309
MRC2_HUMAN	MRC2	physical	25640309
OSGI1_HUMAN	OSGIN1	physical	25640309
HOP2_HUMAN	PSMC3IP	physical	25640309
BRE1A_HUMAN	RNF20	physical	25640309
SG3A1_HUMAN	SCGB3A1	physical	25640309
SPB5_HUMAN	SERPINB5	physical	25640309
SNAI1_HUMAN	SNAI1	physical	25640309
TFF1_HUMAN	TFF1	physical	25640309
TRI25_HUMAN	TRIM25	physical	25640309
DSRAD_HUMAN	ADAR	physical	26496610
A4_HUMAN	APP	physical	26496610
AT2A2_HUMAN	ATP2A2	physical	26496610
ATPA_HUMAN	ATP5A1	physical	26496610
BRCA2_HUMAN	BRCA2	physical	26496610
CALU_HUMAN	CALU	physical	26496610
TCPZ_HUMAN	CCT6A	physical	26496610
CDK9_HUMAN	CDK9	physical	26496610
CLCN7_HUMAN	CLCN7	physical	26496610
COPA_HUMAN	COPA	physical	26496610
CPSM_HUMAN	CPS1	physical	26496610
TIM8A_HUMAN	TIMM8A	physical	26496610
SYFA_HUMAN	FARSA	physical	26496610
FKBP5_HUMAN	FKBP5	physical	26496610
ROA2_HUMAN	HNRNPA2B1	physical	26496610
DNJA1_HUMAN	DNAJA1	physical	26496610
HS90B_HUMAN	HSP90AB1	physical	26496610
CH60_HUMAN	HSPD1	physical	26496610
IMDH1_HUMAN	IMPDH1	physical	26496610
IMDH2_HUMAN	IMPDH2	physical	26496610
TNPO1_HUMAN	TNPO1	physical	26496610
KTN1_HUMAN	KTN1	physical	26496610
METH_HUMAN	MTR	physical	26496610
OPA1_HUMAN	OPA1	physical	26496610
PYC_HUMAN	PC	physical	26496610
PMS2_HUMAN	PMS2	physical	26496610
DPOLB_HUMAN	POLB	physical	26496610
PP1RA_HUMAN	PPP1R10	physical	26496610
SYQ_HUMAN	QARS	physical	26496610
RA51C_HUMAN	RAD51C	physical	26496610
RCN1_HUMAN	RCN1	physical	26496610
RNF5_HUMAN	RNF5	physical	26496610
RL10_HUMAN	RPL10	physical	26496610
AAAT_HUMAN	SLC1A5	physical	26496610
RU17_HUMAN	SNRNP70	physical	26496610
SSRG_HUMAN	SSR3	physical	26496610
TAP1_HUMAN	TAP1	physical	26496610
TCPA_HUMAN	TCP1	physical	26496610
LAP2A_HUMAN	TMPO	physical	26496610
LAP2B_HUMAN	TMPO	physical	26496610
ENPL_HUMAN	HSP90B1	physical	26496610
TCPG_HUMAN	CCT3	physical	26496610
NCOA4_HUMAN	NCOA4	physical	26496610
RBM10_HUMAN	RBM10	physical	26496610
EIF3A_HUMAN	EIF3A	physical	26496610
EIF3F_HUMAN	EIF3F	physical	26496610
RIOK3_HUMAN	RIOK3	physical	26496610
EI2BD_HUMAN	EIF2B4	physical	26496610
ZW10_HUMAN	ZW10	physical	26496610
MED17_HUMAN	MED17	physical	26496610
TECR_HUMAN	TECR	physical	26496610
IF2P_HUMAN	EIF5B	physical	26496610
NUP93_HUMAN	NUP93	physical	26496610
MED24_HUMAN	MED24	physical	26496610
ZC11A_HUMAN	ZC3H11A	physical	26496610
MED16_HUMAN	MED16	physical	26496610
SCAM3_HUMAN	SCAMP3	physical	26496610
CMC2_HUMAN	SLC25A13	physical	26496610
RBM5_HUMAN	RBM5	physical	26496610
SF3A1_HUMAN	SF3A1	physical	26496610
CEPT1_HUMAN	CEPT1	physical	26496610
HAX1_HUMAN	HAX1	physical	26496610
CHERP_HUMAN	CHERP	physical	26496610
ERLN1_HUMAN	ERLIN1	physical	26496610
TCPQ_HUMAN	CCT8	physical	26496610
STIP1_HUMAN	STIP1	physical	26496610
CKAP4_HUMAN	CKAP4	physical	26496610
GCN1_HUMAN	GCN1L1	physical	26496610
SF3B2_HUMAN	SF3B2	physical	26496610
COPE_HUMAN	COPE	physical	26496610
GPN1_HUMAN	GPN1	physical	26496610
PHB2_HUMAN	PHB2	physical	26496610
DNJC8_HUMAN	DNAJC8	physical	26496610
CNDD3_HUMAN	NCAPD3	physical	26496610
UBR4_HUMAN	UBR4	physical	26496610
SF3B1_HUMAN	SF3B1	physical	26496610
FKBP8_HUMAN	FKBP8	physical	26496610
LTN1_HUMAN	LTN1	physical	26496610
ERAL1_HUMAN	ERAL1	physical	26496610
MYOF_HUMAN	MYOF	physical	26496610
TIM13_HUMAN	TIMM13	physical	26496610
QCR8_HUMAN	UQCRQ	physical	26496610
SO4A1_HUMAN	SLCO4A1	physical	26496610
HOOK2_HUMAN	HOOK2	physical	26496610
PI3R4_HUMAN	PIK3R4	physical	26496610
UBR5_HUMAN	UBR5	physical	26496610
EXOC6_HUMAN	EXOC6	physical	26496610
AGGF1_HUMAN	AGGF1	physical	26496610
IPO9_HUMAN	IPO9	physical	26496610
CCAR1_HUMAN	CCAR1	physical	26496610
UN45A_HUMAN	UNC45A	physical	26496610
ERBIN_HUMAN	ERBB2IP	physical	26496610
JPH1_HUMAN	JPH1	physical	26496610
NCLN_HUMAN	NCLN	physical	26496610
TOM22_HUMAN	TOMM22	physical	26496610
PCNP_HUMAN	PCNP	physical	26496610
RCN3_HUMAN	RCN3	physical	26496610
SQOR_HUMAN	SQRDL	physical	26496610
CTF18_HUMAN	CHTF18	physical	26496610
MMS19_HUMAN	MMS19	physical	26496610
SPA5L_HUMAN	SPATA5L1	physical	26496610
DERL1_HUMAN	DERL1	physical	26496610
GEMI7_HUMAN	GEMIN7	physical	26496610
SFXN3_HUMAN	SFXN3	physical	26496610
BACD3_HUMAN	KCTD10	physical	26496610
NSRP1_HUMAN	NSRP1	physical	26496610
HDGR2_HUMAN	HDGFRP2	physical	26496610
SPF45_HUMAN	RBM17	physical	26496610
PPIL4_HUMAN	PPIL4	physical	26496610
TB182_HUMAN	TNKS1BP1	physical	26496610
ZN598_HUMAN	ZNF598	physical	26496610
SFXN1_HUMAN	SFXN1	physical	26496610
CC124_HUMAN	CCDC124	physical	26496610
ADIP_HUMAN	SSX2IP	physical	26496610
BAP18_HUMAN	C17orf49	physical	26496610
FANCA_HUMAN	FANCA	physical	28215707

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
114480	Breast cancer (BC)
613972	Melanoma, cutaneous malignant 6 (CMM6)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of XRCC3_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage."; Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; Science 316:1160-1166(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, AND MASSSPECTROMETRY.	17525332 [Abstract]