RA51C_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RA51C_HUMAN
• UniProt AC: O43502
• Protein Name: DNA repair protein RAD51 homolog 3
• Gene Name: RAD51C
• Organism: Homo sapiens (Human).
• Sequence Length: 376
• Subcellular Localization: Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion. DNA damage induces an increase in nuclear levels. Accumulates in DNA damage induced nuclear foci or RAD51C foci which is formed during the S or G2 phase of cell cycle. Accumulation at
• Protein Description: Essential for the homologous recombination (HR) pathway of DNA repair. Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Part of the RAD21 paralog protein complexes BCDX2 and CX3 which act at different stages of the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 seems to act downstream of BRCA2 recruitment and upstream of RAD51 recruitment; CX3 seems to act downstream of RAD51 recruitment; both complexes bind predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway. Involved in RAD51 foci formation in response to DNA damage suggesting an involvement in early stages of HR probably in the invasion step. Has an early function in DNA repair in facilitating phosphorylation of the checkpoint kinase CHEK2 and thereby transduction of the damage signal, leading to cell cycle arrest and HR activation. Participates in branch migration and HJ resolution and thus is important for processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex. Part of a PALB2-scaffolded HR complex containing BRCA2 and which is thought to play a role in DNA repair by HR. Protects RAD51 from ubiquitin-mediated degradation that is enhanced following DNA damage. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and XRCC3. Contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Involved in maintaining centrosome number in mitosis..
• Protein Sequence: MRGKTFRFEMQRDLVSFPLSPAVRVKLVSAGFQTAEELLEVKPSELSKEVGISKAEALETLQIIRRECLTNKPRYAGTSESHKKCTALELLEQEHTQGFIITFCSALDDILGGGVPLMKTTEICGAPGVGKTQLCMQLAVDVQIPECFGGVAGEAVFIDTEGSFMVDRVVDLATACIQHLQLIAEKHKGEEHRKALEDFTLDNILSHIYYFRCRDYTELLAQVYLLPDFLSEHSKVRLVIVDGIAFPFRHDLDDLSLRTRLLNGLAQQMISLANNHRLAVILTNQMTTKIDRNQALLVPALGESWGHAATIRLIFHWDRKQRLATLYKSPSQKECTVLFQIKPQGFRDTVVTSACSLQTEGSLSTRKRSRDPEEEL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
16	Phosphorylation	EMQRDLVSFPLSPAV EEEHHHHCCCCCHHH	28.57	30266825
20	Phosphorylation	DLVSFPLSPAVRVKL HHHCCCCCHHHHHEE	15.85	30266825
26	Ubiquitination	LSPAVRVKLVSAGFQ CCHHHHHEEEECCCC	32.38	21906983
42	Ubiquitination	AEELLEVKPSELSKE HHHHHCCCHHHHHHH	33.19	21906983
42	Sumoylation	AEELLEVKPSELSKE HHHHHCCCHHHHHHH	33.19	-
48	Ubiquitination	VKPSELSKEVGISKA CCHHHHHHHHCCCHH	69.88	21906983
54	Ubiquitination	SKEVGISKAEALETL HHHHCCCHHHHHHHH	48.61	21906983
60	O-linked_Glycosylation	SKAEALETLQIIRRE CHHHHHHHHHHHHHH	25.47	29237092
72	Ubiquitination	RRECLTNKPRYAGTS HHHHHHCCCCCCCCC	26.15	27667366
72	Acetylation	RRECLTNKPRYAGTS HHHHHHCCCCCCCCC	26.15	25953088
83	Ubiquitination	AGTSESHKKCTALEL CCCCHHHHCCHHHHH	59.84	27667366
84	Ubiquitination	GTSESHKKCTALELL CCCHHHHCCHHHHHH	31.24	22817900
131 (in isoform 2)	Ubiquitination	-	30.59	-
211	Ubiquitination	ILSHIYYFRCRDYTE HHHHHHHHCCCCHHH	3.14	22817900
212	Ubiquitination	LSHIYYFRCRDYTEL HHHHHHHCCCCHHHH	9.84	22817900
216	Ubiquitination	YYFRCRDYTELLAQV HHHCCCCHHHHHHHH	5.24	22817900
217	Ubiquitination	YFRCRDYTELLAQVY HHCCCCHHHHHHHHH	25.17	22817900
256	Phosphorylation	RHDLDDLSLRTRLLN CCCCCCHHHHHHHHH	23.85	24719451
304	Phosphorylation	LVPALGESWGHAATI EEECCCCCCCHHHHE	36.24	29083192
310	Phosphorylation	ESWGHAATIRLIFHW CCCCHHHHEEHEEEC	13.86	29083192
328	Ubiquitination	QRLATLYKSPSQKEC HHHHHHCCCCCCCEE	60.03	22817900
329	Ubiquitination	RLATLYKSPSQKECT HHHHHCCCCCCCEEE	19.07	22817900
333	Ubiquitination	LYKSPSQKECTVLFQ HCCCCCCCEEEEEEE	60.12	33845483
334	Ubiquitination	YKSPSQKECTVLFQI CCCCCCCEEEEEEEE	27.99	22817900
342	Ubiquitination	CTVLFQIKPQGFRDT EEEEEEECCCCCCCE	22.09	-
369	Phosphorylation	SLSTRKRSRDPEEEL CCCCCCCCCCCHHCC	44.03	25690035

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RA51C_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RA51C_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RA51C_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RA51B_HUMAN	RAD51B	physical	15115758
XRCC3_HUMAN	XRCC3	physical	15115758
XRCC2_HUMAN	XRCC2	physical	11744692
XRCC3_HUMAN	XRCC3	physical	11744692
RA51B_HUMAN	RAD51B	physical	11744692
RA51D_HUMAN	RAD51D	physical	11744692
XRCC3_HUMAN	XRCC3	physical	11842113
RA51D_HUMAN	RAD51D	physical	11842113
XRCC2_HUMAN	XRCC2	physical	11842113
RAD51_HUMAN	RAD51	physical	16395335
A4_HUMAN	APP	physical	21832049
SWAP1_HUMAN	SWSAP1	physical	21965664
RA51D_HUMAN	RAD51D	physical	27161866
KLH10_HUMAN	KLHL10	physical	28514442
PTN9_HUMAN	PTPN9	physical	28514442
CC140_HUMAN	CCDC140	physical	28514442
HELQ_HUMAN	HELQ	physical	24005329
RAD51_HUMAN	RAD51	physical	24141787
RA51B_HUMAN	RAD51B	physical	24141787
RA51D_HUMAN	RAD51D	physical	24141787
PALB2_HUMAN	PALB2	physical	24141787
BRCA2_HUMAN	BRCA2	physical	24141787
XRCC2_HUMAN	XRCC2	physical	24141787
XRCC3_HUMAN	XRCC3	physical	24141787

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 613390: Fanconi anemia complementation group O (FANCO)
• 613399: Breast-ovarian cancer, familial, 3 (BROVCA3)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.