TIM8A_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TIM8A_HUMAN
• UniProt AC: O60220
• Protein Name: Mitochondrial import inner membrane translocase subunit Tim8 A
• Gene Name: TIMM8A
• Organism: Homo sapiens (Human).
• Sequence Length: 97
• Subcellular Localization: Mitochondrion inner membrane ; Peripheral membrane protein ; Intermembrane side .
• Protein Description: Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins. Probably necessary for normal neurologic development..
• Protein Sequence: MDSSSSSSAAGLGAVDPQLQHFIEVETQKQRFQQLVHQMTELCWEKCMDKPGPKLDSRAEACFVNCVERFIDTSQFILNRLEQTQKSKPVFSESLSD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MDSSSSSS -------CCCCCCCC	11.56	-
3	Phosphorylation	-----MDSSSSSSAA -----CCCCCCCCCC	30.71	30108239
4	Phosphorylation	----MDSSSSSSAAG ----CCCCCCCCCCC	29.94	30108239
5	Phosphorylation	---MDSSSSSSAAGL ---CCCCCCCCCCCC	37.89	30108239
6	Phosphorylation	--MDSSSSSSAAGLG --CCCCCCCCCCCCC	30.20	30108239
7	Phosphorylation	-MDSSSSSSAAGLGA -CCCCCCCCCCCCCC	26.41	30108239
8	Phosphorylation	MDSSSSSSAAGLGAV CCCCCCCCCCCCCCC	24.88	30108239
57	Phosphorylation	KPGPKLDSRAEACFV CCCCCCCHHHHHHHH	43.77	20833797
86	Ubiquitination	NRLEQTQKSKPVFSE HHHHHHHHCCCCCCC	64.93	21890473
87	Phosphorylation	RLEQTQKSKPVFSES HHHHHHHCCCCCCCC	32.16	30576142
88	Acetylation	LEQTQKSKPVFSESL HHHHHHCCCCCCCCC	52.84	7668055
88	Ubiquitination	LEQTQKSKPVFSESL HHHHHHCCCCCCCCC	52.84	21890473
92	Phosphorylation	QKSKPVFSESLSD-- HHCCCCCCCCCCC--	26.89	21955146
94	Phosphorylation	SKPVFSESLSD---- CCCCCCCCCCC----	31.79	28355574
96	Phosphorylation	PVFSESLSD------ CCCCCCCCC------	51.02	25159151

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TIM8A_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TIM8A_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TIM8A_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
STAM1_HUMAN	STAM	physical	12745081
TIM13_HUMAN	TIMM13	physical	11875042
K1C15_HUMAN	KRT15	physical	25416956
STAM2_HUMAN	STAM2	physical	25416956
TIM10_HUMAN	TIMM10	physical	26344197

Drug and Disease Associations

• Kegg Disease
• OMIM Disease
• 304700: Mohr-Tranebjaerg syndrome (MTS)
• 311150: Jensen syndrome (JENSS)
• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.
PubMed: 19413330

Phosphorylation

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, AND MASSSPECTROMETRY.
PubMed: 18669648