ERF3A_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ERF3A_HUMAN
• UniProt AC: P15170
• Protein Name: Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
• Gene Name: GSPT1
• Organism: Homo sapiens (Human).
• Sequence Length: 499
• Protein Description: Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons..
• Protein Sequence: MELSEPIVENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5 (in isoform 2)	Phosphorylation	-	52.23	28348404
5 (in isoform 3)	Phosphorylation	-	52.23	28348404
13	Phosphorylation	PIVENGETEMSPEES CCCCCCCCCCCHHHH	38.08	26074081
16	Phosphorylation	ENGETEMSPEESWEH CCCCCCCCHHHHHCC	23.95	25159151
17 (in isoform 2)	Phosphorylation	-	48.33	28348404
17 (in isoform 3)	Phosphorylation	-	48.33	28348404
18 (in isoform 2)	Phosphorylation	-	58.31	28348404
18 (in isoform 3)	Phosphorylation	-	58.31	28348404
19 (in isoform 2)	Phosphorylation	-	65.62	28348404
19 (in isoform 3)	Phosphorylation	-	65.62	28348404
20	Phosphorylation	TEMSPEESWEHKEEI CCCCHHHHHCCHHHH	35.78	25159151
21 (in isoform 2)	Phosphorylation	-	18.26	28348404
21 (in isoform 3)	Phosphorylation	-	18.26	28348404
22 (in isoform 2)	Phosphorylation	-	55.12	28348404
22 (in isoform 3)	Phosphorylation	-	55.12	28348404
23 (in isoform 2)	Phosphorylation	-	36.25	28348404
23 (in isoform 3)	Phosphorylation	-	36.25	28348404
24 (in isoform 2)	Phosphorylation	-	45.59	28348404
24 (in isoform 3)	Phosphorylation	-	45.59	28348404
26 (in isoform 2)	Phosphorylation	-	57.53	28348404
26 (in isoform 3)	Phosphorylation	-	57.53	28348404
28	Phosphorylation	WEHKEEISEAEPGGG HCCHHHHCCCCCCCC	33.75	27251275
36	Phosphorylation	EAEPGGGSLGDGRPP CCCCCCCCCCCCCCC	32.24	25849741
46	Phosphorylation	DGRPPEESAHEMMEE CCCCCHHHHHHHHHH	33.01	25159151
49 (in isoform 2)	Phosphorylation	-	39.68	-
49 (in isoform 3)	Phosphorylation	-	39.68	-
62	Phosphorylation	EEIPKPKSVVAPPGA HCCCCCCCCCCCCCC	30.28	28985074
62 (in isoform 2)	Phosphorylation	-	30.28	25159151
62 (in isoform 3)	Phosphorylation	-	30.28	25159151
71	Ubiquitination	VAPPGAPKKEHVNVV CCCCCCCCCCCEEEE	70.97	-
74 (in isoform 2)	Acetylation	-	25.90	-
74 (in isoform 2)	Ubiquitination	-	25.90	-
74 (in isoform 3)	Acetylation	-	25.90	-
74 (in isoform 3)	Ubiquitination	-	25.90	-
88	Phosphorylation	GHVDAGKSTIGGQIM EEECCCCCCHHHHHH	25.08	28634298
88 (in isoform 2)	Phosphorylation	-	25.08	-
88 (in isoform 3)	Phosphorylation	-	25.08	-
89	Phosphorylation	HVDAGKSTIGGQIMY EECCCCCCHHHHHHE	27.42	20068231
96	Phosphorylation	TIGGQIMYLTGMVDK CHHHHHHEEECCCCH	11.62	28634298
98	Phosphorylation	GGQIMYLTGMVDKRT HHHHHEEECCCCHHH	13.02	20068231
108	Acetylation	VDKRTLEKYEREAKE CCHHHHHHHHHHHHH	56.37	23749302
108	Malonylation	VDKRTLEKYEREAKE CCHHHHHHHHHHHHH	56.37	26320211
108	Ubiquitination	VDKRTLEKYEREAKE CCHHHHHHHHHHHHH	56.37	19608861
109	Phosphorylation	DKRTLEKYEREAKEK CHHHHHHHHHHHHHH	15.73	28152594
111	Phosphorylation	RTLEKYEREAKEKNR HHHHHHHHHHHHHCH	46.19	27251275
120	Phosphorylation	AKEKNRETWYLSWAL HHHHCHHHHHEEEEE	19.03	-
124	Phosphorylation	NRETWYLSWALDTNQ CHHHHHEEEEECCCH	8.46	24719451
129	Phosphorylation	YLSWALDTNQEERDK HEEEEECCCHHHHHC	39.38	24719451
138	Ubiquitination	QEERDKGKTVEVGRA HHHHHCCCEEEEHHE	55.95	-
146	Phosphorylation	TVEVGRAYFETEKKH EEEEHHEEEECCCCE	10.69	28152594
152	Ubiquitination	AYFETEKKHFTILDA EEEECCCCEEEEEEC	37.41	-
155	Phosphorylation	ETEKKHFTILDAPGH ECCCCEEEEEECCCC	21.98	28857561
174	Phosphorylation	PNMIGGASQADLAVL CCCCCCCCHHCEEEE	29.22	27251275
187	Ubiquitination	VLVISARKGEFETGF EEEEEECCCCCCCCC	63.61	-
192	Phosphorylation	ARKGEFETGFEKGGQ ECCCCCCCCCCCCCC	53.11	-
196	Acetylation	EFETGFEKGGQTREH CCCCCCCCCCCCHHH	67.11	23236377
196	Malonylation	EFETGFEKGGQTREH CCCCCCCCCCCCHHH	67.11	26320211
196	Methylation	EFETGFEKGGQTREH CCCCCCCCCCCCHHH	67.11	19608861
196	Ubiquitination	EFETGFEKGGQTREH CCCCCCCCCCCCHHH	67.11	19608861
200	Phosphorylation	GFEKGGQTREHAMLA CCCCCCCCHHHHHHH	41.10	24719451
205	Sulfoxidation	GQTREHAMLAKTAGV CCCHHHHHHHHHHCC	3.95	30846556
208	Acetylation	REHAMLAKTAGVKHL HHHHHHHHHHCCCEE	34.64	23749302
208	Ubiquitination	REHAMLAKTAGVKHL HHHHHHHHHHCCCEE	34.64	-
213	Acetylation	LAKTAGVKHLIVLIN HHHHHCCCEEEEEEE	31.60	25953088
213	Malonylation	LAKTAGVKHLIVLIN HHHHHCCCEEEEEEE	31.60	26320211
222	Sulfoxidation	LIVLINKMDDPTVNW EEEEEECCCCCCCCC	6.46	30846556
227	Phosphorylation	NKMDDPTVNWSNERY ECCCCCCCCCCCHHH	9.34	-
236	Phosphorylation	WSNERYEECKEKLVP CCCHHHHHHHHHHHH	41.79	-
238	Acetylation	NERYEECKEKLVPFL CHHHHHHHHHHHHHH	62.25	23749302
240	Acetylation	RYEECKEKLVPFLKK HHHHHHHHHHHHHHH	40.04	23236377
240	Ubiquitination	RYEECKEKLVPFLKK HHHHHHHHHHHHHHH	40.04	-
245	Acetylation	KEKLVPFLKKVGFNP HHHHHHHHHHCCCCC	4.26	19608861
246	Acetylation	EKLVPFLKKVGFNPK HHHHHHHHHCCCCCC	45.88	23236377
246	Malonylation	EKLVPFLKKVGFNPK HHHHHHHHHCCCCCC	45.88	26320211
247	Acetylation	KLVPFLKKVGFNPKK HHHHHHHHCCCCCCC	50.14	26051181
247	Malonylation	KLVPFLKKVGFNPKK HHHHHHHHCCCCCCC	50.14	26320211
253	Acetylation	KKVGFNPKKDIHFMP HHCCCCCCCCEEEEE	65.27	23749302
253	Malonylation	KKVGFNPKKDIHFMP HHCCCCCCCCEEEEE	65.27	26320211
253	Ubiquitination	KKVGFNPKKDIHFMP HHCCCCCCCCEEEEE	65.27	-
254	Malonylation	KVGFNPKKDIHFMPC HCCCCCCCCEEEEEC	64.27	26320211
254	Ubiquitination	KVGFNPKKDIHFMPC HCCCCCCCCEEEEEC	64.27	-
262	Phosphorylation	DIHFMPCSGLTGANL CEEEEECCCCCCCCH	30.85	24719451
267	Phosphorylation	PCSGLTGANLKEQSD ECCCCCCCCHHHCCC	16.97	24719451
289 (in isoform 2)	Ubiquitination	-	49.10	-
309	Acetylation	IRLPIVDKYKDMGTV CCCCCEECCCCCCEE	43.22	26051181
309	Ubiquitination	IRLPIVDKYKDMGTV CCCCCEECCCCCCEE	43.22	-
313	Sulfoxidation	IVDKYKDMGTVVLGK CEECCCCCCEEEEEE	4.19	21406390
315	Phosphorylation	DKYKDMGTVVLGKLE ECCCCCCEEEEEECC	10.78	-
320	Acetylation	MGTVVLGKLESGSIC CCEEEEEECCCCCCC	44.94	25953088
320	Ubiquitination	MGTVVLGKLESGSIC CCEEEEEECCCCCCC	44.94	-
328	Ubiquitination	LESGSICKGQQLVMM CCCCCCCCCEEEEEC	59.07	-
333	Acetylation	ICKGQQLVMMPNKHN CCCCEEEEECCCCCC	2.41	19608861
333	Ubiquitination	ICKGQQLVMMPNKHN CCCCEEEEECCCCCC	2.41	19608861
334	Acetylation	CKGQQLVMMPNKHNV CCCEEEEECCCCCCE	5.13	-
334	Sulfoxidation	CKGQQLVMMPNKHNV CCCEEEEECCCCCCE	5.13	30846556
335	Sulfoxidation	KGQQLVMMPNKHNVE CCEEEEECCCCCCEE	2.29	30846556
363	Ubiquitination	VAPGENLKIRLKGIE CCCCCCEEEEECCCC	37.04	-
367	Ubiquitination	ENLKIRLKGIEEEEI CCEEEEECCCCHHHC	48.25	-
377 (in isoform 2)	Ubiquitination	-	20.84	-
381	S-palmitoylation	ILPGFILCDPNNLCH CCCCEEEECCCCCCC	7.55	29575903
387	S-palmitoylation	LCDPNNLCHSGRTFD EECCCCCCCCCCCCC	2.33	29575903
436	O-linked_Glycosylation	ICLVDKKSGEKSKTR EEEEECCCCCCCCCC	58.90	30379171
448	Acetylation	KTRPRFVKQDQVCIA CCCCCCCCHHHHHHH	45.50	23236377
448	Malonylation	KTRPRFVKQDQVCIA CCCCCCCCHHHHHHH	45.50	26320211
448	Ubiquitination	KTRPRFVKQDQVCIA CCCCCCCCHHHHHHH	45.50	-
453	S-nitrosocysteine	FVKQDQVCIARLRTA CCCHHHHHHHHHHCC	1.35	-
453	S-nitrosylation	FVKQDQVCIARLRTA CCCHHHHHHHHHHCC	1.35	19483679
457 (in isoform 2)	Ubiquitination	-	4.16	-
469	Acetylation	TICLETFKDFPQMGR EEEEEECCCCCCCCC	66.92	26822725
469	Ubiquitination	TICLETFKDFPQMGR EEEEEECCCCCCCCC	66.92	-
478	Phosphorylation	FPQMGRFTLRDEGKT CCCCCCEEECCCCCE	21.37	24719451
484	Ubiquitination	FTLRDEGKTIAIGKV EEECCCCCEEEEEEE	34.41	-
490	Acetylation	GKTIAIGKVLKLVPE CCEEEEEEEEEECCC	37.98	23236377
490	Malonylation	GKTIAIGKVLKLVPE CCEEEEEEEEEECCC	37.98	33225896
490	Ubiquitination	GKTIAIGKVLKLVPE CCEEEEEEEEEECCC	37.98	21890473
493	Acetylation	IAIGKVLKLVPEKD- EEEEEEEEECCCCC-	50.88	19608861
493	Malonylation	IAIGKVLKLVPEKD- EEEEEEEEECCCCC-	50.88	26320211
493	Ubiquitination	IAIGKVLKLVPEKD- EEEEEEEEECCCCC-	50.88	19608861
504 (in isoform 2)	Ubiquitination	-		-
616	Phosphorylation	---------------------------------------------------------------------------------------------------------------------------- ----------------------------------------------------------------------------------------------------------------------------		24719451
627	Acetylation	--------------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------------		19608861
627	Ubiquitination	--------------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------------		19608861
627	Ubiquitination	--------------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------------		21890473
628	Acetylation	---------------------------------------------------------------------------------------------------------------------------------------- ----------------------------------------------------------------------------------------------------------------------------------------		-
630	Acetylation	------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------		19608861
631	Acetylation	------------------------------------------------------------------------------------------------------------------------------------------- -------------------------------------------------------------------------------------------------------------------------------------------		-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ERF3A_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ERF3A_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ERF3A_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
XIAP_HUMAN	XIAP	physical	12865429
SPTN1_HUMAN	SPTAN1	physical	22939629
TCPB_HUMAN	CCT2	physical	22939629
RENT1_HUMAN	UPF1	physical	18256688
PABP1_HUMAN	PABPC1	physical	18256688
ERF1_HUMAN	ETF1	physical	18256688
P85A_HUMAN	PIK3R1	physical	21988832
ERF3B_HUMAN	GSPT2	physical	22863883
RTF1_HUMAN	RTF1	physical	22863883
SNX2_HUMAN	SNX2	physical	22863883
EF2_HUMAN	EEF2	physical	26344197
PABP1_HUMAN	PABPC1	physical	26344197
PABP4_HUMAN	PABPC4	physical	26344197
RUVB1_HUMAN	RUVBL1	physical	26344197
XIAP_HUMAN	XIAP	physical	26172506

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.