WDR4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: WDR4_HUMAN
• UniProt AC: P57081
• Protein Name: tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 {ECO:0000255|HAMAP-Rule:MF_03056}
• Gene Name: WDR4 {ECO:0000255|HAMAP-Rule:MF_03056}
• Organism: Homo sapiens (Human).
• Sequence Length: 412
• Subcellular Localization: Nucleus .
• Protein Description: Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit..
• Protein Sequence: MAGSVGLALCGQTLVVRGGSRFLATSIASSDDDSLFIYDCSAAEKKSQENKGEDAPLDQGSGAILASTFSKSGSYFALTDDSKRLILFRTKPWQCLSVRTVARRCTALTFIASEEKVLVADKSGDVYSFSVLEPHGCGRLELGHLSMLLDVAVSPDDRFILTADRDEKIRVSWAAAPHSIESFCLGHTEFVSRISVVPTQPGLLLSSSGDGTLRLWEYRSGRQLHCCHLASLQELVDPQAPQKFAASRIAFWCQENCVALLCDGTPVVYIFQLDARRQQLVYRQQLAFQHQVWDVAFEETQGLWVLQDCQEAPLVLYRPVGDQWQSVPESTVLKKVSGVLRGNWAMLEGSAGADASFSSLYKATFDNVTSYLKKKEERLQQQLEKKQRRRSPPPGPDGHAKKMRPGEATLSC

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAGSVGLAL ------CCCCHHEEE	20.97	-
4	Phosphorylation	----MAGSVGLALCG ----CCCCHHEEECC	11.95	24043423
13	Phosphorylation	GLALCGQTLVVRGGS HEEECCCEEEEECCC	13.55	24043423
51	Ubiquitination	EKKSQENKGEDAPLD HHHHHCCCCCCCCCC	64.03	29967540
106	Phosphorylation	RTVARRCTALTFIAS HHHHHHCCHHHEEEC	23.92	29449344
109	Phosphorylation	ARRCTALTFIASEEK HHHCCHHHEEECCCE	15.55	29449344
113	Phosphorylation	TALTFIASEEKVLVA CHHHEEECCCEEEEE	41.28	29449344
168	Acetylation	LTADRDEKIRVSWAA EECCCCCCEEEEEEE	39.85	26051181
245	Phosphorylation	PQAPQKFAASRIAFW CCCCHHHHHHHHHHH	16.15	33259812
326	Phosphorylation	PVGDQWQSVPESTVL ECCCCCCCCCHHHHH	36.36	30576142
337	Phosphorylation	STVLKKVSGVLRGNW HHHHHHHHCCEECCE	31.87	24732914
358	Phosphorylation	AGADASFSSLYKATF CCCCCCHHHHHHHHH	19.55	-
390	Phosphorylation	LEKKQRRRSPPPGPD HHHHHHHCCCCCCCC	56.56	33259812
391	Phosphorylation	EKKQRRRSPPPGPDG HHHHHHCCCCCCCCC	39.69	29255136
409	Phosphorylation	KMRPGEATLSC---- CCCCCCCCCCC----	18.20	25159151
411	Phosphorylation	RPGEATLSC------ CCCCCCCCC------	17.27	25159151

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of WDR4_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of WDR4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of WDR4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ARI1_HUMAN	ARIH1	physical	22863883
KBP_HUMAN	KIAA1279	physical	22863883
OGT1_HUMAN	OGT	physical	22863883
SRP14_HUMAN	SRP14	physical	22863883
XPO5_HUMAN	XPO5	physical	22863883
TRMB_HUMAN	METTL1	physical	15861136
F118A_HUMAN	FAM118A	physical	25416956
BIRC6_HUMAN	BIRC6	physical	26186194
DPP8_HUMAN	DPP8	physical	26186194
2ABA_HUMAN	PPP2R2A	physical	26344197
SAR1A_HUMAN	SAR1A	physical	26344197
FEN1_HUMAN	FEN1	physical	26751069
TRMB_HUMAN	METTL1	physical	26751069
PCNA_HUMAN	PCNA	physical	26751069
H2B1B_HUMAN	HIST1H2BB	physical	26751069
RS16_HUMAN	RPS16	physical	26751069
ALG12_HUMAN	ALG12	physical	28514442
RN138_HUMAN	RNF138	physical	28514442
PBX2_HUMAN	PBX2	physical	28514442
XPP1_HUMAN	XPNPEP1	physical	28514442
TCAL1_HUMAN	TCEAL1	physical	28514442
CARM1_HUMAN	CARM1	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-411, AND MASSSPECTROMETRY.
PubMed: 19413330

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-411, AND MASSSPECTROMETRY.
PubMed: 18669648

"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks.";
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.;
Cell 127:635-648(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-391, AND MASSSPECTROMETRY.
PubMed: 17081983