dbPTM

AFAD_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	AFAD_HUMAN
UniProt AC	P55196
Protein Name	Afadin
Gene Name	AFDN {ECO:0000312\|HGNC:HGNC:7137}
Organism	Homo sapiens (Human).
Sequence Length	1824
Subcellular Localization	Cell junction, adherens junction. Not found at cell-matrix AJs.
Protein Description	Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton..
Protein Sequence	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSAGGRDEE ------CCCCCCHHH	47.10	29255136
91	Phosphorylation	RMLSSPKYSLYEVHV HHHCCCCCEEEEEEE	13.80	28152594
92	Phosphorylation	MLSSPKYSLYEVHVS HHCCCCCEEEEEEEC	30.51	28442448
94	Phosphorylation	SSPKYSLYEVHVSGE CCCCCEEEEEEECCC	15.23	20007894
99	Phosphorylation	SLYEVHVSGERRLDI EEEEEEECCCCCCCC	21.76	28152594
160	Phosphorylation	VIQNFKRTLSKKEKK HHHHHHHHHCHHHHH	35.65	20363803
162	Phosphorylation	QNFKRTLSKKEKKEK HHHHHHHCHHHHHHH	40.86	20363803
178	Methylation	KREKEALRQASDKDD HHHHHHHHHCCCCCC	36.75	-
181	Phosphorylation	KEALRQASDKDDRPF HHHHHHCCCCCCCCC	36.53	29255136
203	Phosphorylation	SRLAAEVYKDMPETS HHHHHHHHCCCCCCC	7.87	25884760
209	Phosphorylation	VYKDMPETSFTRTIS HHCCCCCCCCEECCC	24.22	29514088
210	Phosphorylation	YKDMPETSFTRTISN HCCCCCCCCEECCCC	23.98	26356563
212	Phosphorylation	DMPETSFTRTISNPE CCCCCCCEECCCCHH	27.03	26356563
214	Phosphorylation	PETSFTRTISNPEVV CCCCCEECCCCHHHH	26.21	30266825
216	Phosphorylation	TSFTRTISNPEVVMK CCCEECCCCHHHHHH	45.80	19664994
239	Phosphorylation	KRMQEFRSSDGRPDS HHHHHHHCCCCCCCC	37.79	29255136
240	Phosphorylation	RMQEFRSSDGRPDSG HHHHHHCCCCCCCCC	39.57	29255136
246	Phosphorylation	SSDGRPDSGGTLRIY CCCCCCCCCCEEEEE	41.63	25849741
249	Phosphorylation	GRPDSGGTLRIYADS CCCCCCCEEEEEECC	19.18	29255136
253	Phosphorylation	SGGTLRIYADSLKPN CCCEEEEEECCCCCC	9.64	28152594
256	Phosphorylation	TLRIYADSLKPNIPY EEEEEECCCCCCCCC	30.07	28152594
263	Phosphorylation	SLKPNIPYKTILLST CCCCCCCCEEEEEEC	19.57	25884760
269	Phosphorylation	PYKTILLSTTDPADF CCEEEEEECCCHHHH	26.30	20068231
270	Phosphorylation	YKTILLSTTDPADFA CEEEEEECCCHHHHH	34.88	28348404
271	Phosphorylation	KTILLSTTDPADFAV EEEEEECCCHHHHHH	36.29	28348404
295	Phosphorylation	EKENPKDYCIARVML CCCCCCCCEEEEEEC	7.59	28152594
324	Glutathionylation	IILDDDECPLQIFRE EECCCCCCCCHHHHH	5.40	22555962
372 (in isoform 1)	Phosphorylation	-	23.22	29978859
372 (in isoform 2)	Phosphorylation	-	23.22	29978859
372 (in isoform 3)	Phosphorylation	-	23.22	29978859
373	Phosphorylation	GKERADGSGYGSTLP CCCCCCCCCCCCCCC	29.41	25002506
374 (in isoform 1)	Phosphorylation	-	36.03	29978859
374 (in isoform 2)	Phosphorylation	-	36.03	29978859
374 (in isoform 3)	Phosphorylation	-	36.03	29978859
375	Phosphorylation	ERADGSGYGSTLPPE CCCCCCCCCCCCCHH	15.03	18180459
376 (in isoform 1)	Phosphorylation	-	20.06	24719451
376 (in isoform 2)	Phosphorylation	-	20.06	24719451
376 (in isoform 3)	Phosphorylation	-	20.06	24719451
377	Phosphorylation	ADGSGYGSTLPPEKL CCCCCCCCCCCHHHC	20.49	25002506
377 (in isoform 1)	Phosphorylation	-	20.49	29978859
377 (in isoform 2)	Phosphorylation	-	20.49	29978859
377 (in isoform 3)	Phosphorylation	-	20.49	29978859
378	Phosphorylation	DGSGYGSTLPPEKLP CCCCCCCCCCHHHCC	38.63	25002506
386	Phosphorylation	LPPEKLPYLVELSPG CCHHHCCEEEEECCC	33.54	24117733
390 (in isoform 1)	Phosphorylation	-	6.12	27732954
390 (in isoform 2)	Phosphorylation	-	6.12	27732954
390 (in isoform 3)	Phosphorylation	-	6.12	27732954
391	Phosphorylation	LPYLVELSPGRRNHF CCEEEEECCCCCCCC	16.29	24117733
394 (in isoform 1)	Phosphorylation	-	39.34	27732954
394 (in isoform 2)	Phosphorylation	-	39.34	27732954
394 (in isoform 3)	Phosphorylation	-	39.34	27732954
396 (in isoform 1)	Phosphorylation	-	33.01	27732954
396 (in isoform 2)	Phosphorylation	-	33.01	27732954
396 (in isoform 3)	Phosphorylation	-	33.01	27732954
400	Phosphorylation	GRRNHFAYYNYHTYE CCCCCCEEEEEEECC	7.41	26552605
401	Phosphorylation	RRNHFAYYNYHTYED CCCCCEEEEEEECCC	13.05	26552605
403	Phosphorylation	NHFAYYNYHTYEDGS CCCEEEEEEECCCCC	4.57	26552605
405	Phosphorylation	FAYYNYHTYEDGSDS CEEEEEEECCCCCCC	20.46	26552605
406	Phosphorylation	AYYNYHTYEDGSDSR EEEEEEECCCCCCCC	9.94	26552605
410	Phosphorylation	YHTYEDGSDSRDKPK EEECCCCCCCCCCCC	44.74	26552605
412	Phosphorylation	TYEDGSDSRDKPKLY ECCCCCCCCCCCCEE	44.04	26552605
424	Phosphorylation	KLYRLQLSVTEVGTE CEEEEEEEEEECCCC	17.65	19664994
426	Phosphorylation	YRLQLSVTEVGTEKL EEEEEEEEECCCCCC	22.90	166220815
430	Phosphorylation	LSVTEVGTEKLDDNS EEEEECCCCCCCCCC	34.81	30108239
481	Phosphorylation	GQRISETTMLQSGMK CEEECCCEECCCCCE	16.31	25332170
485	Phosphorylation	SETTMLQSGMKVQFG CCCEECCCCCEEEEC	36.57	25332170
512	Phosphorylation	DHALAKRSVDGGLMV CCHHHHHCCCCCEEE	24.69	30266825
532	Phosphorylation	KPGIVQETTFDLGGD CCCCCEEEEEECCCC	18.70	24719451
533	Phosphorylation	PGIVQETTFDLGGDI CCCCEEEEEECCCCC	17.88	28857561
533 (in isoform 3)	Phosphorylation	-	17.88	18669648
542	Phosphorylation	DLGGDIHSGTALPTS ECCCCCCCCCCCCCC	37.87	28348404
544	Phosphorylation	GGDIHSGTALPTSKS CCCCCCCCCCCCCCC	28.21	28348404
548	Phosphorylation	HSGTALPTSKSTTRL CCCCCCCCCCCCCCC	50.03	26074081
549	Phosphorylation	SGTALPTSKSTTRLD CCCCCCCCCCCCCCC	23.75	18669648
551	Phosphorylation	TALPTSKSTTRLDSD CCCCCCCCCCCCCCC	34.48	22496350
552	Phosphorylation	ALPTSKSTTRLDSDR CCCCCCCCCCCCCCC	21.48	26074081
553	Phosphorylation	LPTSKSTTRLDSDRV CCCCCCCCCCCCCCC	36.00	26074081
557	Phosphorylation	KSTTRLDSDRVSSAS CCCCCCCCCCCCCCC	31.72	25159151
561	Phosphorylation	RLDSDRVSSASSTAE CCCCCCCCCCCHHHC	22.53	29255136
562	Phosphorylation	LDSDRVSSASSTAER CCCCCCCCCCHHHCC	29.34	29255136
564	Phosphorylation	SDRVSSASSTAERGM CCCCCCCCHHHCCCC	30.12	29255136
565	Phosphorylation	DRVSSASSTAERGMV CCCCCCCHHHCCCCC	31.21	29255136
566	Phosphorylation	RVSSASSTAERGMVK CCCCCCHHHCCCCCC	30.00	29255136
584	Phosphorylation	RVEQQPDYRRQESRT EEECCCCCCCCCCCC	17.87	20007894
589	Phosphorylation	PDYRRQESRTQDASG CCCCCCCCCCCCCCC	32.08	22617229
591	O-linked_Glycosylation	YRRQESRTQDASGPE CCCCCCCCCCCCCCC	39.83	30379171
591	Phosphorylation	YRRQESRTQDASGPE CCCCCCCCCCCCCCC	39.83	28450419
595	Phosphorylation	ESRTQDASGPELILP CCCCCCCCCCCEEEE	64.27	28450419
604	Phosphorylation	PELILPASIEFRESS CCEEEECEEEEECCC	22.25	23186163
644	Phosphorylation	VLYMACRYVLSNQYR HHHHHHHHHHHCCCC	12.96	23186163
647	Phosphorylation	MACRYVLSNQYRPDI HHHHHHHHCCCCCCC	16.31	23186163
650	Phosphorylation	RYVLSNQYRPDISPT HHHHHCCCCCCCCCC	28.50	27732954
655	Phosphorylation	NQYRPDISPTERTHK CCCCCCCCCCCCCHH	32.90	25850435
657	Phosphorylation	YRPDISPTERTHKVI CCCCCCCCCCCHHHH	31.17	25850435
672	Phosphorylation	AVVNKMVSMMEGVIQ HHHHHHHHHHHHHHH	14.65	50563831
844	Phosphorylation	LAADCHLSRIVQATT HHHHCCHHHHHHHHH	9.04	28509920
850	Phosphorylation	LSRIVQATTLLTMDK HHHHHHHHHHHCCCC	10.82	29978859
851	Phosphorylation	SRIVQATTLLTMDKY HHHHHHHHHHCCCCC	23.84	29978859
854	Phosphorylation	VQATTLLTMDKYAPD HHHHHHHCCCCCCCC	26.83	29978859
858	Phosphorylation	TLLTMDKYAPDDIPN HHHCCCCCCCCCCCC	21.07	29978859
868	Phosphorylation	DDIPNINSTCFKLNS CCCCCCCHHHHHHCH	23.82	29978859
869	Phosphorylation	DIPNINSTCFKLNSL CCCCCCHHHHHHCHH	19.92	29978859
893	Phosphorylation	HCAPDEPFIPTDLIE CCCCCCCCCCHHHHH	11.13	12808105
909	Phosphorylation	VVTVAENTADELARS HHHHHHHHHHHHHHC	27.30	12808105
942	Glutathionylation	LPEDGYSCDVVRNIP CCCCCCCCCEECCCC	3.29	22555962
973	Phosphorylation	RLIPHTRSPGTWTIY EECCCCCCCCEEEEE	28.92	69008947
976	Phosphorylation	PHTRSPGTWTIYFEG CCCCCCCEEEEEECC	23.87	69008949
980	Phosphorylation	SPGTWTIYFEGADYE CCCEEEEEECCCCHH	6.55	45553647
986	Phosphorylation	IYFEGADYESHLLRE EEECCCCHHHHHHHC	20.85	4857643
988	Phosphorylation	FEGADYESHLLRENT ECCCCHHHHHHHCCC	17.13	29978859
995	Phosphorylation	SHLLRENTELAQPLR HHHHHCCCHHCCCHH	27.85	28857561
1009	O-linked_Glycosylation	RKEPEIITVTLKKQN HCCCCEEEEEEECCC	17.07	30379171
1009	Phosphorylation	RKEPEIITVTLKKQN HCCCCEEEEEEECCC	17.07	23312004
1011	Phosphorylation	EPEIITVTLKKQNGM CCCEEEEEEECCCCC	24.98	23312004
1014	Methylation	IITVTLKKQNGMGLS EEEEEEECCCCCCEE	52.70	-
1036	Phosphorylation	GQDKLGIYVKSVVKG CCCCCEEEEEEEECC	10.46	22210691
1039	Phosphorylation	KLGIYVKSVVKGGAA CCEEEEEEEECCCCC	23.31	22210691
1060	Phosphorylation	AAGDQLLSVDGRSLV CCCCEEEEECCCHHH	27.10	24719451
1070	Phosphorylation	GRSLVGLSQERAAEL CCHHHCCCHHHHHHH	24.86	30624053
1081	Phosphorylation	AAELMTRTSSVVTLE HHHHHHHCCCEEEHH	19.04	30266825
1082	Phosphorylation	AELMTRTSSVVTLEV HHHHHHCCCEEEHHH	20.18	19664994
1083	Phosphorylation	ELMTRTSSVVTLEVA HHHHHCCCEEEHHHH	21.72	19664994
1086	Phosphorylation	TRTSSVVTLEVAKQG HHCCCEEEHHHHHCC	18.29	30266825
1090	Phosphorylation	SVVTLEVAKQGAIYH CEEEHHHHHCCHHHH	6.78	17081983
1096	Phosphorylation	VAKQGAIYHGLATLL HHHCCHHHHHHHHHH	6.84	23403867
1101	Phosphorylation	AIYHGLATLLNQPSP HHHHHHHHHHCCCCH	36.50	68705041
1107	Phosphorylation	ATLLNQPSPMMQRIS HHHHCCCCHHHHHHH	18.70	29255136
1114	Phosphorylation	SPMMQRISDRRGSGK CHHHHHHHCCCCCCC	27.53	27422710
1117	Methylation	MQRISDRRGSGKPRP HHHHHCCCCCCCCCC	48.93	-
1119	Phosphorylation	RISDRRGSGKPRPKS HHHCCCCCCCCCCCC	41.66	59152037
1126	Phosphorylation	SGKPRPKSEGFELYN CCCCCCCCCCCEECC	45.71	29978859
1132	Phosphorylation	KSEGFELYNNSTQNG CCCCCEECCCCCCCC	12.71	29978859
1135	Phosphorylation	GFELYNNSTQNGSPE CCEECCCCCCCCCCC	27.94	29978859
1136	Phosphorylation	FELYNNSTQNGSPES CEECCCCCCCCCCCC	28.33	26657352
1140	Phosphorylation	NNSTQNGSPESPQLP CCCCCCCCCCCCCCC	32.80	28102081
1143	Phosphorylation	TQNGSPESPQLPWAE CCCCCCCCCCCCCHH	22.72	22617229
1151	Phosphorylation	PQLPWAEYSEPKKLP CCCCCHHCCCCCCCC	15.58	29978859
1152	Phosphorylation	QLPWAEYSEPKKLPG CCCCHHCCCCCCCCC	38.64	29978859
1155	Phosphorylation	WAEYSEPKKLPGDDR CHHCCCCCCCCCCCH	63.99	15144186
1156	Phosphorylation	AEYSEPKKLPGDDRL HHCCCCCCCCCCCHH	71.30	15144186
1165	Phosphorylation	PGDDRLMKNRADHRS CCCCHHHHCCCCCCC	48.12	15144186
1172	Phosphorylation	KNRADHRSSPNVANQ HCCCCCCCCCCCCCC	46.88	29255136
1173	Phosphorylation	NRADHRSSPNVANQP CCCCCCCCCCCCCCC	22.05	29255136
1182	Phosphorylation	NVANQPPSPGGKSAY CCCCCCCCCCCCCCC	42.74	19664994
1184	Phosphorylation	ANQPPSPGGKSAYAS CCCCCCCCCCCCCCC	61.57	15302935
1186	Methylation	QPPSPGGKSAYASGT CCCCCCCCCCCCCCC	37.52	-
1187	Phosphorylation	PPSPGGKSAYASGTT CCCCCCCCCCCCCCE	29.77	23312004
1189	Phosphorylation	SPGGKSAYASGTTAK CCCCCCCCCCCCEEE	14.37	28152594
1191	Phosphorylation	GGKSAYASGTTAKIT CCCCCCCCCCEEEEE	24.81	23312004
1194	Phosphorylation	SAYASGTTAKITSVS CCCCCCCEEEEEEEE	29.49	30576142
1198	Phosphorylation	SGTTAKITSVSTGNL CCCEEEEEEEECCCC	23.51	30183078
1199	Phosphorylation	GTTAKITSVSTGNLC CCEEEEEEEECCCCC	19.97	25159151
1201	Phosphorylation	TAKITSVSTGNLCTE EEEEEEEECCCCCCC	30.29	31693
1202	Phosphorylation	AKITSVSTGNLCTEE EEEEEEECCCCCCCC	28.12	30183078
1207	Phosphorylation	VSTGNLCTEEQTPPP EECCCCCCCCCCCCC	45.80	23898821
1211	Phosphorylation	NLCTEEQTPPPRPEA CCCCCCCCCCCCCCC	40.83	25159151
1219	Phosphorylation	PPPRPEAYPIPTQTY CCCCCCCCCCCCCCE	10.47	28450419
1223	Phosphorylation	PEAYPIPTQTYTREY CCCCCCCCCCEEEEE	33.97	30576142
1225	Phosphorylation	AYPIPTQTYTREYFT CCCCCCCCEEEEEEE	29.37	29978859
1226	Phosphorylation	YPIPTQTYTREYFTF CCCCCCCEEEEEEEE	8.25	30576142
1227	Phosphorylation	PIPTQTYTREYFTFP CCCCCCEEEEEEEEC	22.03	30576142
1229	Phosphorylation	PTQTYTREYFTFPAS CCCCEEEEEEEECCC	36.88	17016520
1230	Phosphorylation	TQTYTREYFTFPASK CCCEEEEEEEECCCC	12.48	30266825
1232	Phosphorylation	TYTREYFTFPASKSQ CEEEEEEEECCCCCC	28.01	19664994
1236	Phosphorylation	EYFTFPASKSQDRMA EEEEECCCCCCCCCC	32.83	30266825
1238	O-linked_Glycosylation	FTFPASKSQDRMAPP EEECCCCCCCCCCCC	34.51	30379171
1238	Phosphorylation	FTFPASKSQDRMAPP EEECCCCCCCCCCCC	34.51	23898821
1252	Phosphorylation	PQNQWPNYEEKPHMH CCCCCCCCCCCCCCC	22.76	27259358
1260	Phosphorylation	EEKPHMHTDSNHSSI CCCCCCCCCCCCHHH	33.28	27696853
1262	Phosphorylation	KPHMHTDSNHSSIAI CCCCCCCCCCHHHHH	37.28	20363803
1265	Phosphorylation	MHTDSNHSSIAIQRV CCCCCCCHHHHHHHH	27.91	20363803
1266	Phosphorylation	HTDSNHSSIAIQRVT CCCCCCHHHHHHHHH	14.42	101545355
1273	Phosphorylation	SIAIQRVTRSQEELR HHHHHHHHCCHHHHH	27.13	22617229
1275	Phosphorylation	AIQRVTRSQEELRED HHHHHHCCHHHHHCC	32.36	29255136
1285	Phosphorylation	ELREDKAYQLERHRI HHHCCCCHHHHHHHH	21.26	29978859
1300	Phosphorylation	EAAMDRKSDSDMWIN HHHHHCCCCCCCCCC	43.15	23927012
1302	Phosphorylation	AMDRKSDSDMWINQS HHHCCCCCCCCCCCC	36.13	25849741
1309	Phosphorylation	SDMWINQSSSLDSST CCCCCCCCCCCCCCC	19.87	30266825
1310	Phosphorylation	DMWINQSSSLDSSTS CCCCCCCCCCCCCCC	25.75	30266825
1311	Phosphorylation	MWINQSSSLDSSTSS CCCCCCCCCCCCCCC	41.25	30266825
1314	Phosphorylation	NQSSSLDSSTSSQEH CCCCCCCCCCCCHHH	40.59	30266825
1315	Phosphorylation	QSSSLDSSTSSQEHL CCCCCCCCCCCHHHH	31.79	30266825
1316	Phosphorylation	SSSLDSSTSSQEHLN CCCCCCCCCCHHHHC	36.03	30266825
1317	Phosphorylation	SSLDSSTSSQEHLNH CCCCCCCCCHHHHCC	31.78	30266825
1318	Phosphorylation	SLDSSTSSQEHLNHS CCCCCCCCHHHHCCC	39.41	30266825
1325	Phosphorylation	SQEHLNHSSKSVTPA CHHHHCCCCCCCCCC	38.09	20363803
1326	Phosphorylation	QEHLNHSSKSVTPAS HHHHCCCCCCCCCCH	22.77	30266825
1328	Phosphorylation	HLNHSSKSVTPASTL HHCCCCCCCCCCHHC	32.92	30266825
1330	Phosphorylation	NHSSKSVTPASTLTK CCCCCCCCCCHHCCC	21.89	30266825
1333	Phosphorylation	SKSVTPASTLTKSGP CCCCCCCHHCCCCCC	25.75	30266825
1334	Phosphorylation	KSVTPASTLTKSGPG CCCCCCHHCCCCCCC	40.32	30266825
1336	Phosphorylation	VTPASTLTKSGPGRW CCCCHHCCCCCCCCC	24.21	29255136
1345	Phosphorylation	SGPGRWKTPAAIPAT CCCCCCCCCCCCCCC	15.94	27794612
1352	Phosphorylation	TPAAIPATPVAVSQP CCCCCCCCCEEECCC	16.97	25159151
1357	Phosphorylation	PATPVAVSQPIRTDL CCCCEEECCCCCCCC	22.09	27794612
1419	Phosphorylation	REEHQRWYEKEKARL HHHHHHHHHHHHHHH	21.22	7947869
1446	Phosphorylation	RKLGQMRTQSLNPAP HHHHHHHHCCCCCCC	19.71	23403867
1448	Phosphorylation	LGQMRTQSLNPAPFS HHHHHHCCCCCCCCC	29.51	28348404
1455	Phosphorylation	SLNPAPFSPLTAQQM CCCCCCCCCCCHHHC	20.24	21584817
1458	Phosphorylation	PAPFSPLTAQQMKPE CCCCCCCCHHHCCCC	26.29	27732954
1468	Phosphorylation	QMKPEKPSTLQRPQE HCCCCCCCCCCCCHH	53.47	29116813
1469	Phosphorylation	MKPEKPSTLQRPQET CCCCCCCCCCCCHHH	35.12	23403867
1476	Phosphorylation	TLQRPQETVIRELQP CCCCCHHHHHHHCCC	18.91	27251275
1489	Phosphorylation	QPQQQPRTIERRDLQ CCCCCCCCCCHHCCE	33.60	23403867
1497	Phosphorylation	IERRDLQYITVSKEE CCHHCCEEEEEEHHH	13.07	21945579
1499	Phosphorylation	RRDLQYITVSKEELS HHCCEEEEEEHHHHC	17.50	21945579
1501	Phosphorylation	DLQYITVSKEELSSG CCEEEEEEHHHHCCC	26.21	30266825
1506	Phosphorylation	TVSKEELSSGDSLSP EEEHHHHCCCCCCCC	35.24	23403867
1507	Phosphorylation	VSKEELSSGDSLSPD EEHHHHCCCCCCCCC	60.28	23403867
1510	Phosphorylation	EELSSGDSLSPDPWK HHHCCCCCCCCCCCH	33.98	30266825
1512	Phosphorylation	LSSGDSLSPDPWKRD HCCCCCCCCCCCHHH	31.07	19664994
1545	Phosphorylation	KEIQELQSKPDRSAE HHHHHHHCCCCCCHH	59.80	29083192
1656	Phosphorylation	KRRQEEGYYSRLEAE HHHHHHHHHHHHHHH	11.10	23403867
1657	Phosphorylation	RRQEEGYYSRLEAER HHHHHHHHHHHHHHH	9.73	23403867
1658	Phosphorylation	RQEEGYYSRLEAERR HHHHHHHHHHHHHHH	23.16	23403867
1681	Phosphorylation	RLLEPEAPGLCRPPL HHHCCCCCCCCCCCC	34.33	16964243
1692	Phosphorylation	RPPLPRDYEPPSPSP CCCCCCCCCCCCCCC	31.45	30266825
1696	Phosphorylation	PRDYEPPSPSPAPGA CCCCCCCCCCCCCCC	49.04	30266825
1698	Phosphorylation	DYEPPSPSPAPGAPP CCCCCCCCCCCCCCC	37.72	30266825
1713	Phosphorylation	PPPQRNASYLKTQVL CCCCCCHHHEEEEEC	34.25	24719451
1716	Methylation	QRNASYLKTQVLSPD CCCHHHEEEEECCHH	28.78	-
1717	Phosphorylation	RNASYLKTQVLSPDS CCHHHEEEEECCHHH	22.70	24732914
1721	Phosphorylation	YLKTQVLSPDSLFTA HEEEEECCHHHHHEE	28.01	19664994
1724	Phosphorylation	TQVLSPDSLFTAKFV EEECCHHHHHEEEEE	28.85	30266825
1727	Phosphorylation	LSPDSLFTAKFVAYN CCHHHHHEEEEEECC	33.94	30266825
1739 (in isoform 1)	Phosphorylation	-	63.52	26657352
1749	Phosphorylation	CSLAGPNSYPGSTGA CCCCCCCCCCCCCCC	35.52	28985074
1753	Phosphorylation	GPNSYPGSTGAAVGA CCCCCCCCCCCCCCH	21.11	-
1761 (in isoform 1)	Phosphorylation	-	19.78	25850435
1772	Phosphorylation	RDAKEKRSKSQDADS HHHHHHHHHCCCCCC	47.96	23927012
1774	Phosphorylation	AKEKRSKSQDADSPG HHHHHHHCCCCCCCC	34.24	29255136
1779	Phosphorylation	SKSQDADSPGSSGAP HHCCCCCCCCCCCCC	32.64	19664994
1782	Phosphorylation	QDADSPGSSGAPENL CCCCCCCCCCCCCCC	29.05	29255136
1783	Phosphorylation	DADSPGSSGAPENLT CCCCCCCCCCCCCCC	44.91	36753417
1790	Phosphorylation	SGAPENLTFKERQRL CCCCCCCCHHHHHHH	44.22	24732914
1799	Phosphorylation	KERQRLFSQGQDVSN HHHHHHHHCCCCHHH	37.68	19664994
1805	Phosphorylation	FSQGQDVSNKVKASR HHCCCCHHHHHHHHH	38.94	30266825
1807	Acetylation	QGQDVSNKVKASRKL CCCCHHHHHHHHHHH	37.29	25953088
1815	Phosphorylation	VKASRKLTELENELN HHHHHHHHHHHHHHC	41.37	25159151
1823	Phosphorylation	ELENELNTK------ HHHHHHCCC------	51.24	29255136

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
893	T	Phosphorylation	Kinase	BCR	P11274	GPS
909	T	Phosphorylation	Kinase	BCR	P11274	PSP
1352	T	Phosphorylation	Kinase	CDK2	P24941	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of AFAD_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of AFAD_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
BCR_HUMAN	BCR	physical	12808105
RPGP1_HUMAN	RAP1GAP	physical	12590145
NECT1_HUMAN	PVRL1	physical	10225955
PROF1_HUMAN	PFN1	physical	10922060
RASN_HUMAN	NRAS	physical	12808105
ADIP_HUMAN	SSX2IP	physical	12446711
RBTN2_HUMAN	LMO2	physical	12067721
USP9X_HUMAN	USP9X	physical	10620020
EPHB2_HUMAN	EPHB2	physical	9707552
EPHB3_HUMAN	EPHB3	physical	9707552
EPHB6_HUMAN	EPHB6	physical	9707552
EPHA7_HUMAN	EPHA7	physical	9707552
NRX1B_HUMAN	NRXN1	physical	9707552
NRX1A_HUMAN	NRXN1	physical	9707552
NRX2B_HUMAN	NRXN2	physical	9707552
NRX2A_HUMAN	NRXN2	physical	9707552
NRX3B_HUMAN	NRXN3	physical	9707552
NRX3A_HUMAN	NRXN3	physical	9707552
JAG1_HUMAN	JAG1	physical	9707552
SRBS1_HUMAN	SORBS1	physical	10085297
NECT3_HUMAN	PVRL3	physical	10744716
NECT3_HUMAN	PVRL3	physical	11024295
USP9X_HUMAN	USP9X	physical	9722616
MEN1_HUMAN	MEN1	physical	16239140
PRKN_HUMAN	PARK2	physical	23393160
NR2C2_HUMAN	NR2C2	physical	26344197
KI13B_HUMAN	KIF13B	physical	27173435
GGYF1_HUMAN	GIGYF1	physical	27173435
LRFN1_HUMAN	LRFN1	physical	27173435
SI1L1_HUMAN	SIPA1L1	physical	27173435
MAGI1_HUMAN	MAGI1	physical	27173435
TESK2_HUMAN	TESK2	physical	27173435
DCLK1_HUMAN	DCLK1	physical	27173435
SRS12_HUMAN	SRSF12	physical	27173435
SYDE1_HUMAN	SYDE1	physical	27173435
F110B_HUMAN	FAM110B	physical	27173435
F110A_HUMAN	FAM110A	physical	27173435
CBY1_HUMAN	CBY1	physical	27173435

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of AFAD_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216; SER-1083; SER-1182;SER-1721 AND SER-1799.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216; SER-1172; SER-1173;SER-1182; THR-1330; SER-1512 AND SER-1721, AND MASS SPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216; SER-1107; SER-1182;THR-1232; SER-1721; SER-1779 AND SER-1799.	18669648 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1696, AND MASSSPECTROMETRY.	18220336 [Abstract]
"Quantitative phosphoproteome profiling of Wnt3a-mediated signalingnetwork: indicating the involvement of ribonucleoside-diphosphatereductase M2 subunit phosphorylation at residue serine 20 in canonicalWnt signal transduction."; Tang L.-Y., Deng N., Wang L.-S., Dai J., Wang Z.-L., Jiang X.-S.,Li S.-J., Li L., Sheng Q.-H., Wu D.-Q., Li L., Zeng R.; Mol. Cell. Proteomics 6:1952-1967(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1182, AND MASSSPECTROMETRY.	17693683 [Abstract]
"A probability-based approach for high-throughput proteinphosphorylation analysis and site localization."; Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.; Nat. Biotechnol. 24:1285-1292(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1698, AND MASSSPECTROMETRY.	16964243 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1107, AND MASSSPECTROMETRY.	17081983 [Abstract]
"Large-scale characterization of HeLa cell nuclear phosphoproteins."; Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1182 AND SER-1201, ANDMASS SPECTROMETRY.	15302935 [Abstract]
"Robust phosphoproteomic profiling of tyrosine phosphorylation sitesfrom human T cells using immobilized metal affinity chromatography andtandem mass spectrometry."; Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M.,Peters E.C.; Anal. Chem. 76:2763-2772(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1172 AND SER-1173, ANDMASS SPECTROMETRY.	15144186 [Abstract]
"Multiple reaction monitoring for robust quantitative proteomicanalysis of cellular signaling networks."; Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M.; Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1230, AND MASSSPECTROMETRY.	17389395 [Abstract]
"Global survey of phosphotyrosine signaling identifies oncogenickinases in lung cancer."; Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J.,Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L.,Mitchell J., Wetzel R., Macneill J., Ren J.M., Yuan J.,Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X.,Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.; Cell 131:1190-1203(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-94, AND MASSSPECTROMETRY.	18083107 [Abstract]