dbPTM

ASAP1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ASAP1_HUMAN
UniProt AC	Q9ULH1
Protein Name	Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
Gene Name	ASAP1
Organism	Homo sapiens (Human).
Sequence Length	1129
Subcellular Localization	Cytoplasm. Membrane. Predominantly cytoplasmic. Partially membrane-associated..
Protein Description	Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis..
Protein Sequence	MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSTSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTVLHYCSMYSKPECLKLLLRSKPTVDIVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEIDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPTTEAPPLPPRNAGKGPTGPPSTLPLSTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAVPWGNDGGPSSSSKTTNKFEGLSQQSSTSSAKTALGPRVLPKLPQKVALRKTDHLSLDKATIPPEIFQKSSQLAELPQKPPPGDLPPKPTELAPKPQIGDLPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQTGDVSPKAQQPSEVTLKSHPLDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MRSSASRLSS -----CCCHHHHHHH	41.96	26270265
4	Phosphorylation	----MRSSASRLSSF ----CCCHHHHHHHC	36.17	26270265
6	Phosphorylation	--MRSSASRLSSFSS --CCCHHHHHHHCCC	35.38	26270265
9	Phosphorylation	RSSASRLSSFSSRDS CCHHHHHHHCCCCHH	28.38	23403867
10	Phosphorylation	SSASRLSSFSSRDSL CHHHHHHHCCCCHHH	33.47	23403867
12	Phosphorylation	ASRLSSFSSRDSLWN HHHHHHCCCCHHHHH	26.81	23403867
13	Phosphorylation	SRLSSFSSRDSLWNR HHHHHCCCCHHHHHH	37.79	23403867
34	Phosphorylation	VSEFIAETTEDYNSP HHHHHHHCCCCCCCC	26.85	-
79	Malonylation	QKVKKSVKAIYNSGQ HHHHHHHHHHHHCCC	35.84	26320211
101	Ubiquitination	NYAQVLDKFGSNFLS HHHHHHHHHHHHHHC	47.68	-
129	Phosphorylation	STLTKELSTLLKNLL HHHHHHHHHHHHHHH	20.35	23403867
130	Phosphorylation	TLTKELSTLLKNLLQ HHHHHHHHHHHHHHH	49.05	23403867
149	Phosphorylation	NVIFTLDSLLKGDLK HHEEEHHHHHCCCCC	38.42	24719451
182	Acetylation	TKFTKIEKEKREHAK HHHHHHHHHHHHHHH	72.51	-
184	Acetylation	FTKIEKEKREHAKQH HHHHHHHHHHHHHHH	74.32	130655
209	Ubiquitination	EIAEEMEKERRLFQL HHHHHHHHHHHHHHH	56.03	-
232	Ubiquitination	VNEIKTKKGVDLLQN HHHCCCHHHHHHHHH	70.20	-
256	Ubiquitination	NFFQDGLKTADKLKQ CCHHHHHHHHHHHHH	47.39	-
276	Ubiquitination	AADLYNIKQTQDEEK HHHHHCCCCCCCHHH	43.59	-
284	Ubiquitination	QTQDEEKKQLTALRD CCCCHHHHHHHHHHH	54.54	-
287	Phosphorylation	DEEKKQLTALRDLIK CHHHHHHHHHHHHHH	22.58	24719451
305	Phosphorylation	QLDQKEDSQSRQGGY CCCCCCHHHCCCCCC	31.02	28857561
307	Phosphorylation	DQKEDSQSRQGGYSM CCCCHHHCCCCCCCH	30.44	28857561
310 (in isoform 2)	Phosphorylation	-	35.58	29978859
312	Phosphorylation	SQSRQGGYSMHQLQG HHCCCCCCCHHHHCC	15.05	25159151
315 (in isoform 2)	Phosphorylation	-	21.18	29978859
316 (in isoform 2)	Phosphorylation	-	27.17	29978859
330	Phosphorylation	YGSEKKGYLLKKSDG HCCCCCCEEEECCCC	20.10	29341593
372	Phosphorylation	PAKLNLLTCQVKPNA CEEEEEEEEECCCCC	12.21	-
409	Phosphorylation	QDYVAWISVLTNSKE CCEEEEEEEECCCHH	10.60	22210691
412	Phosphorylation	VAWISVLTNSKEEAL EEEEEEECCCHHHHH	34.98	22210691
428	Phosphorylation	MAFRGEQSAGENSLE HHHCCCCCCCCCCHH	33.66	20068231
433	Phosphorylation	EQSAGENSLEDLTKA CCCCCCCCHHHHHHH	28.91	27251275
493	Phosphorylation	VHISRIQSLELDKLG CCHHHEEEEEHHHCC	22.80	30266825
501	Phosphorylation	LELDKLGTSELLLAK EEHHHCCCCHHHHHH	29.48	17322306
502	Phosphorylation	ELDKLGTSELLLAKN EHHHCCCCHHHHHHC	25.17	17322306
514	Phosphorylation	AKNVGNNSFNDIMEA HHCCCCCCHHHHHHC	29.44	24076635
525	Phosphorylation	IMEANLPSPSPKPTP HHHCCCCCCCCCCCC	40.97	25850435
527	Phosphorylation	EANLPSPSPKPTPSS HCCCCCCCCCCCCCC	50.31	25850435
555	Acetylation	VDHRFSRKTCSTSSA CCHHHCCCCCCCCHH	53.32	30588015
556	Phosphorylation	DHRFSRKTCSTSSAK CHHHCCCCCCCCHHH	15.58	23312004
558	Phosphorylation	RFSRKTCSTSSAKLN HHCCCCCCCCHHHHH	36.23	23312004
559	Phosphorylation	FSRKTCSTSSAKLNE HCCCCCCCCHHHHHH	28.93	23312004
560	Phosphorylation	SRKTCSTSSAKLNEL CCCCCCCCHHHHHHH	16.41	23312004
561	Phosphorylation	RKTCSTSSAKLNELL CCCCCCCHHHHHHHH	29.52	23312004
563	Acetylation	TCSTSSAKLNELLEA CCCCCHHHHHHHHHH	54.52	30588021
648	Phosphorylation	LHYCSMYSKPECLKL HHHHHHCCCHHHHHH	32.85	24719451
662	Phosphorylation	LLLRSKPTVDIVNQA HHHHCCCCCEEECCC	34.25	24247654
672	Phosphorylation	IVNQAGETALDIAKR EECCCCHHHHHHHHH	31.44	24247654
705	Phosphorylation	NPHVHVEYEWNLRQE CCCCEEEEEEECCHH	25.75	28270605
717	Phosphorylation	RQEEIDESDDDLDDK CHHHCCCCCCCCCCC	42.46	23927012
726	Phosphorylation	DDLDDKPSPIKKERS CCCCCCCCCCCCCCC	45.02	23927012
733	Phosphorylation	SPIKKERSPRPQSFC CCCCCCCCCCCCCCC	26.76	23927012
738	Phosphorylation	ERSPRPQSFCHSSSI CCCCCCCCCCCCCCC	32.30	25159151
742	Phosphorylation	RPQSFCHSSSISPQD CCCCCCCCCCCCHHH	27.28	23927012
743	Phosphorylation	PQSFCHSSSISPQDK CCCCCCCCCCCHHHC	14.18	23401153
744	Phosphorylation	QSFCHSSSISPQDKL CCCCCCCCCCHHHCC	30.05	25159151
746	Phosphorylation	FCHSSSISPQDKLAL CCCCCCCCHHHCCCC	20.61	25159151
757	Phosphorylation	KLALPGFSTPRDKQR CCCCCCCCCCCHHHH	43.34	29255136
758	Phosphorylation	LALPGFSTPRDKQRL CCCCCCCCCCHHHHC	21.78	29255136
767	Phosphorylation	RDKQRLSYGAFTNQI CHHHHCCCCCEECEE	19.64	-
777	Phosphorylation	FTNQIFVSTSTDSPT EECEEEEEECCCCCC	13.09	26074081
778	Phosphorylation	TNQIFVSTSTDSPTS ECEEEEEECCCCCCC	30.28	26657352
779	Phosphorylation	NQIFVSTSTDSPTSP CEEEEEECCCCCCCC	23.78	26074081
780	Phosphorylation	QIFVSTSTDSPTSPT EEEEEECCCCCCCCC	40.35	26074081
782	Phosphorylation	FVSTSTDSPTSPTTE EEEECCCCCCCCCCC	30.11	26074081
784	Phosphorylation	STSTDSPTSPTTEAP EECCCCCCCCCCCCC	51.82	26074081
785	Phosphorylation	TSTDSPTSPTTEAPP ECCCCCCCCCCCCCC	24.51	26074081
787	Phosphorylation	TDSPTSPTTEAPPLP CCCCCCCCCCCCCCC	36.72	26074081
788	Phosphorylation	DSPTSPTTEAPPLPP CCCCCCCCCCCCCCC	33.27	26074081
800	Ubiquitination	LPPRNAGKGPTGPPS CCCCCCCCCCCCCCC	60.34	-
803	Phosphorylation	RNAGKGPTGPPSTLP CCCCCCCCCCCCCCC	71.62	26074081
807	Phosphorylation	KGPTGPPSTLPLSTQ CCCCCCCCCCCCCEE	45.79	29978859
808	Phosphorylation	GPTGPPSTLPLSTQT CCCCCCCCCCCCEEC	37.86	29978859
812	Phosphorylation	PPSTLPLSTQTSSGS CCCCCCCCEECCCCC	19.27	26657352
813	Phosphorylation	PSTLPLSTQTSSGSS CCCCCCCEECCCCCC	43.48	28450419
815	Phosphorylation	TLPLSTQTSSGSSTL CCCCCEECCCCCCCC	25.58	28450419
816	Phosphorylation	LPLSTQTSSGSSTLS CCCCEECCCCCCCCC	23.92	26657352
817	Phosphorylation	PLSTQTSSGSSTLSK CCCEECCCCCCCCCC	46.19	28450419
819	Phosphorylation	STQTSSGSSTLSKKR CEECCCCCCCCCCCC	23.30	28450419
820	Phosphorylation	TQTSSGSSTLSKKRP EECCCCCCCCCCCCC	36.57	28450419
821	Phosphorylation	QTSSGSSTLSKKRPP ECCCCCCCCCCCCCC	36.43	28450419
823	Phosphorylation	SSGSSTLSKKRPPPP CCCCCCCCCCCCCCC	35.90	28450419
824	Ubiquitination	SGSSTLSKKRPPPPP CCCCCCCCCCCCCCC	56.78	-
837	Phosphorylation	PPPGHKRTLSDPPSP CCCCCCCCCCCCCCC	35.10	22167270
839	Phosphorylation	PGHKRTLSDPPSPLP CCCCCCCCCCCCCCC	47.67	22167270
843	Phosphorylation	RTLSDPPSPLPHGPP CCCCCCCCCCCCCCC	44.44	25159151
864	Phosphorylation	WGNDGGPSSSSKTTN CCCCCCCCCCCCCCC	46.24	22199227
865	Phosphorylation	GNDGGPSSSSKTTNK CCCCCCCCCCCCCCC	41.59	21815630
866	Phosphorylation	NDGGPSSSSKTTNKF CCCCCCCCCCCCCCC	39.94	22199227
867	Phosphorylation	DGGPSSSSKTTNKFE CCCCCCCCCCCCCCC	35.65	22199227
877	Phosphorylation	TNKFEGLSQQSSTSS CCCCCCCCCCCCCCC	36.71	29507054
880	Phosphorylation	FEGLSQQSSTSSAKT CCCCCCCCCCCCCHH	28.13	28857561
881	Phosphorylation	EGLSQQSSTSSAKTA CCCCCCCCCCCCHHH	28.15	28985074
883	Phosphorylation	LSQQSSTSSAKTALG CCCCCCCCCCHHHCC	30.19	28985074
887	Phosphorylation	SSTSSAKTALGPRVL CCCCCCHHHCCCCCC	27.17	28985074
905	Acetylation	PQKVALRKTDHLSLD CHHHHCCCCCCCCCC	60.19	-
906	Phosphorylation	QKVALRKTDHLSLDK HHHHCCCCCCCCCCC	23.40	30266825
910	Phosphorylation	LRKTDHLSLDKATIP CCCCCCCCCCCCCCC	30.97	30266825
915	Phosphorylation	HLSLDKATIPPEIFQ CCCCCCCCCCHHHHH	39.49	18077418
924	Phosphorylation	PPEIFQKSSQLAELP CHHHHHCHHHHHCCC	16.72	18077418
925	Phosphorylation	PEIFQKSSQLAELPQ HHHHHCHHHHHCCCC	35.93	18077418
978	Phosphorylation	LPPKPQLSDLPPKPQ CCCCCCHHCCCCCCC	31.67	26074081
1002	Phosphorylation	LGDLLAKSQTGDVSP HHHHHHHHCCCCCCC	27.63	29396449
1004	Phosphorylation	DLLAKSQTGDVSPKA HHHHHHCCCCCCCCC	42.30	30242111
1008	Phosphorylation	KSQTGDVSPKAQQPS HHCCCCCCCCCCCCC	25.98	25159151
1015	Phosphorylation	SPKAQQPSEVTLKSH CCCCCCCCCEEECCC	40.12	26074081
1018	Phosphorylation	AQQPSEVTLKSHPLD CCCCCCEEECCCCCC	25.15	24043423
1021	Phosphorylation	PSEVTLKSHPLDLSP CCCEEECCCCCCCCC	33.19	23403867
1027	Phosphorylation	KSHPLDLSPNVQSRD CCCCCCCCCCCCCHH	17.69	25159151
1032	Phosphorylation	DLSPNVQSRDAIQKQ CCCCCCCCHHHHHHH	28.04	22617229
1041	Phosphorylation	DAIQKQASEDSNDLT HHHHHHHHCCCCCCC	39.17	23401153
1044	Phosphorylation	QKQASEDSNDLTPTL HHHHHCCCCCCCCCC	28.35	30266825
1048	Phosphorylation	SEDSNDLTPTLPETP HCCCCCCCCCCCCCC	19.66	30278072
1050	Phosphorylation	DSNDLTPTLPETPVP CCCCCCCCCCCCCCC	50.11	28450419
1054	Phosphorylation	LTPTLPETPVPLPRK CCCCCCCCCCCCCCC	27.87	25159151
1097	Phosphorylation	EGEVIIVTGEEDQEW EEEEEEEECCCCCEE	28.59	-
1122	Phosphorylation	RKGVFPVSFVHILSD CCCEECEEEEEECCC	23.07	23403867
1128	Phosphorylation	VSFVHILSD------ EEEEEECCC------	40.44	25159151

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of ASAP1_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ASAP1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ASAP1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
REPS2_MOUSE	Reps2	physical	12149250
ARF6_HUMAN	ARF6	physical	18094045
SH3K1_HUMAN	SH3KBP1	physical	17255943
CBL_HUMAN	CBL	physical	17255943
CBLB_HUMAN	CBLB	physical	17255943
SRC8_HUMAN	CTTN	physical	17893324
SRC8_HUMAN	CTTN	physical	15719014
PAXI_HUMAN	PXN	physical	16636290
GRB2_HUMAN	GRB2	physical	21988832
RFIP3_HUMAN	RAB11FIP3	physical	18685082
ARF1_HUMAN	ARF1	physical	16332543

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ASAP1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-843, AND MASSSPECTROMETRY.	19690332 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-839; SER-843 ANDSER-1027, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1027, AND MASSSPECTROMETRY.	18220336 [Abstract]
"Phosphoproteome of resting human platelets."; Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J.,Schuetz C., Walter U., Gambaryan S., Sickmann A.; J. Proteome Res. 7:526-534(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-839; SER-843 ANDSER-1008, AND MASS SPECTROMETRY.	18088087 [Abstract]