dbPTM

RHG04_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RHG04_HUMAN
UniProt AC	P98171
Protein Name	Rho GTPase-activating protein 4 {ECO:0000305}
Gene Name	ARHGAP4 {ECO:0000312\|HGNC:HGNC:674}
Organism	Homo sapiens (Human).
Sequence Length	946
Subcellular Localization	Cytoplasm. Just below the plasma membrane.
Protein Description	Inhibitory effect on stress fiber organization. May down-regulate Rho-like GTPase in hematopoietic cells..
Protein Sequence	MAAHGKLRRERGLQAEYETQVKEMRWQLSEQLRCLELQGELRRELLQELAEFMRRRAEVELEYSRGLEKLAERFSSRGGRLGSSREHQSFRKEPSLLSPLHCWAVLLQHTRQQSRESAALSEVLAGPLAQRLSHIAEDVGRLVKKSRDLEQQLQDELLEVVSELQTAKKTYQAYHMESVNAEAKLREAERQEEKRAGRSVPTTTAGATEAGPLRKSSLKKGGRLVEKRQAKFMEHKLKCTKARNEYLLSLASVNAAVSNYYLHDVLDLMDCCDTGFHLALGQVLRSYTAAESRTQASQVQGLGSLEEAVEALDPPGDKAKVLEVHATVFCPPLRFDYHPHDGDEVAEICVEMELRDEILPRAQNIQSRLDRQTIETEEVNKTLKATLQALLEVVASDDGDVLDSFQTSPSTESLKSTSSDPGSRQAGRRRGQQQETETFYLTKLQEYLSGRSILAKLQAKHEKLQEALQRGDKEEQEVSWTQYTQRKFQKSRQPRPSSQYNQRLFGGDMEKFIQSSGQPVPLVVESCIRFINLNGLQHEGIFRVSGAQLRVSEIRDAFERGEDPLVEGCTAHDLDSVAGVLKLYFRSLEPPLFPPDLFGELLASSELEATAERVEHVSRLLWRLPAPVLVVLRYLFTFLNHLAQYSDENMMDPYNLAVCFGPTLLPVPAGQDPVALQGRVNQLVQTLIVQPDRVFPPLTSLPGPVYEKCMAPPSASCLGDAQLESLGADNEPELEAEMPAQEDDLEGVVEAVACFAYTGRTAQELSFRRGDVLRLHERASSDWWRGEHNGMRGLIPHKYITLPAGTEKQVVGAGLQTAGESGSSPEGLLASELVHRPEPCTSPEAMGPSGHRRRCLVPASPEQHVEVDKAVAQNMDSVFKELLGKTSVRQGLGPASTTSPSPGPRSPKAPPSSRLGRNKGFSRGPGAPASPSASHPQGLDTTPKPH

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Acetylation	--MAAHGKLRRERGL --CCCCHHHHHHHCC	29.02	25953088
17	Phosphorylation	ERGLQAEYETQVKEM HHCCCCHHHHHHHHH	27.56	28796482
19	Phosphorylation	GLQAEYETQVKEMRW CCCCHHHHHHHHHHH	37.45	28796482
22	Ubiquitination	AEYETQVKEMRWQLS CHHHHHHHHHHHHHH	35.66	-
22 (in isoform 2)	Ubiquitination	-	35.66	-
69	Acetylation	EYSRGLEKLAERFSS HHHHHHHHHHHHHHC	59.02	25953088
69	Ubiquitination	EYSRGLEKLAERFSS HHHHHHHHHHHHHHC	59.02	-
69 (in isoform 2)	Ubiquitination	-	59.02	-
83	Phosphorylation	SRGGRLGSSREHQSF CCCCCCCCCCCCHHH	30.71	-
89	Phosphorylation	GSSREHQSFRKEPSL CCCCCCHHHCCCCCC	29.00	27251275
92	Ubiquitination	REHQSFRKEPSLLSP CCCHHHCCCCCCCCH	72.47	-
92 (in isoform 2)	Ubiquitination	-	72.47	-
168	Ubiquitination	VSELQTAKKTYQAYH HHHHHHHHHHHHHHH	49.38	29967540
169	Ubiquitination	SELQTAKKTYQAYHM HHHHHHHHHHHHHHH	50.42	29967540
169 (in isoform 2)	Ubiquitination	-	50.42	-
171	Phosphorylation	LQTAKKTYQAYHMES HHHHHHHHHHHHHHH	10.46	17053785
184	Ubiquitination	ESVNAEAKLREAERQ HHHCHHHHHHHHHHH	39.31	29967540
184 (in isoform 2)	Ubiquitination	-	39.31	-
199	O-linked_Glycosylation	EEKRAGRSVPTTTAG HHHHCCCCCCCCCCC	31.37	28657654
199	Phosphorylation	EEKRAGRSVPTTTAG HHHHCCCCCCCCCCC	31.37	23312004
202	O-linked_Glycosylation	RAGRSVPTTTAGATE HCCCCCCCCCCCCCC	34.32	28657654
202	Phosphorylation	RAGRSVPTTTAGATE HCCCCCCCCCCCCCC	34.32	23312004
203	O-linked_Glycosylation	AGRSVPTTTAGATEA CCCCCCCCCCCCCCC	14.47	28657654
203	Phosphorylation	AGRSVPTTTAGATEA CCCCCCCCCCCCCCC	14.47	23312004
204	O-linked_Glycosylation	GRSVPTTTAGATEAG CCCCCCCCCCCCCCC	26.10	28657654
204	Phosphorylation	GRSVPTTTAGATEAG CCCCCCCCCCCCCCC	26.10	23312004
208	O-linked_Glycosylation	PTTTAGATEAGPLRK CCCCCCCCCCCCCCH	26.00	28657654
208	Phosphorylation	PTTTAGATEAGPLRK CCCCCCCCCCCCCCH	26.00	23312004
216	Phosphorylation	EAGPLRKSSLKKGGR CCCCCCHHHHHHCCC	34.17	24247654
217	Phosphorylation	AGPLRKSSLKKGGRL CCCCCHHHHHHCCCH	47.44	22115753
236	Ubiquitination	QAKFMEHKLKCTKAR HHHHHHHHHHCCHHH	36.01	-
276 (in isoform 2)	Ubiquitination	-	4.66	-
278 (in isoform 2)	Ubiquitination	-	3.82	-
292	Phosphorylation	RSYTAAESRTQASQV HHHHHHHHHHHHHHC	35.58	22210691
318	Ubiquitination	ALDPPGDKAKVLEVH HHCCCCCCCEEEEEE	56.90	-
320	Ubiquitination	DPPGDKAKVLEVHAT CCCCCCCEEEEEEEE	53.76	-
376	Phosphorylation	LDRQTIETEEVNKTL HHHHCCCHHHHHHHH	33.33	-
381	Ubiquitination	IETEEVNKTLKATLQ CCHHHHHHHHHHHHH	61.48	-
384	Ubiquitination	EEVNKTLKATLQALL HHHHHHHHHHHHHHH	44.55	-
396	Phosphorylation	ALLEVVASDDGDVLD HHHHHHHCCCCCCHH	25.32	28060719
404	Phosphorylation	DDGDVLDSFQTSPST CCCCCHHHCCCCCCC	18.32	28060719
407	Phosphorylation	DVLDSFQTSPSTESL CCHHHCCCCCCCCHH	40.36	28060719
408	Phosphorylation	VLDSFQTSPSTESLK CHHHCCCCCCCCHHH	13.18	26074081
410	Phosphorylation	DSFQTSPSTESLKST HHCCCCCCCCHHHCC	44.57	26074081
411	Phosphorylation	SFQTSPSTESLKSTS HCCCCCCCCHHHCCC	32.74	26657352
413	Phosphorylation	QTSPSTESLKSTSSD CCCCCCCHHHCCCCC	40.98	26074081
417	Phosphorylation	STESLKSTSSDPGSR CCCHHHCCCCCCCCH	30.57	30242111
418	Phosphorylation	TESLKSTSSDPGSRQ CCHHHCCCCCCCCHH	39.57	26552605
419	Phosphorylation	ESLKSTSSDPGSRQA CHHHCCCCCCCCHHH	48.16	26552605
421 (in isoform 2)	Ubiquitination	-	44.61	-
423	Phosphorylation	STSSDPGSRQAGRRR CCCCCCCCHHHHHHC	26.97	26552605
443	Ubiquitination	TETFYLTKLQEYLSG HHHHHHHHHHHHHCC	45.65	22817900
447	Phosphorylation	YLTKLQEYLSGRSIL HHHHHHHHHCCHHHH	8.00	29449344
448	Phosphorylation	LTKLQEYLSGRSILA HHHHHHHHCCHHHHH	4.27	24719451
449	Phosphorylation	TKLQEYLSGRSILAK HHHHHHHCCHHHHHH	31.12	29449344
450	Phosphorylation	KLQEYLSGRSILAKL HHHHHHCCHHHHHHH	25.43	27251275
451	Phosphorylation	LQEYLSGRSILAKLQ HHHHHCCHHHHHHHH	19.69	27251275
452	Phosphorylation	QEYLSGRSILAKLQA HHHHCCHHHHHHHHH	26.02	29449344
456	Ubiquitination	SGRSILAKLQAKHEK CCHHHHHHHHHHHHH	37.37	29967540
463	Ubiquitination	KLQAKHEKLQEALQR HHHHHHHHHHHHHHH	56.01	29967540
473	Ubiquitination	EALQRGDKEEQEVSW HHHHHCCHHHHHCCH	67.01	29967540
479	Phosphorylation	DKEEQEVSWTQYTQR CHHHHHCCHHHHHHH	24.53	28857561
481	Phosphorylation	EEQEVSWTQYTQRKF HHHHCCHHHHHHHHH	12.28	29978859
483	Phosphorylation	QEVSWTQYTQRKFQK HHCCHHHHHHHHHHH	9.47	29978859
483	Ubiquitination	QEVSWTQYTQRKFQK HHCCHHHHHHHHHHH	9.47	21890473
483 (in isoform 2)	Ubiquitination	-	9.47	-
483	Ubiquitination	QEVSWTQYTQRKFQK HHCCHHHHHHHHHHH	9.47	21890473
484	Phosphorylation	EVSWTQYTQRKFQKS HCCHHHHHHHHHHHC	16.45	29978859
496	Ubiquitination	QKSRQPRPSSQYNQR HHCCCCCCCHHHHHH	45.04	29967540
496 (in isoform 2)	Ubiquitination	-	45.04	-
497	Phosphorylation	KSRQPRPSSQYNQRL HCCCCCCCHHHHHHH	31.21	23403867
498	Phosphorylation	SRQPRPSSQYNQRLF CCCCCCCHHHHHHHH	39.09	23403867
500	Phosphorylation	QPRPSSQYNQRLFGG CCCCCHHHHHHHHCC	18.40	23312004
503	Ubiquitination	PSSQYNQRLFGGDME CCHHHHHHHHCCCHH	28.48	29967540
503 (in isoform 2)	Ubiquitination	-	28.48	-
511	Ubiquitination	LFGGDMEKFIQSSGQ HHCCCHHHHHHHCCC	40.92	-
513	Ubiquitination	GGDMEKFIQSSGQPV CCCHHHHHHHCCCCC	6.02	29967540
513 (in isoform 2)	Ubiquitination	-	6.02	-
515	Phosphorylation	DMEKFIQSSGQPVPL CHHHHHHHCCCCCCC	31.45	28787133
516	Phosphorylation	MEKFIQSSGQPVPLV HHHHHHHCCCCCCCH	25.68	28787133
519	Phosphorylation	FIQSSGQPVPLVVES HHHHCCCCCCCHHHH	32.97	27251275
523	Phosphorylation	SGQPVPLVVESCIRF CCCCCCCHHHHHHHH	3.53	27642862
526	Phosphorylation	PVPLVVESCIRFINL CCCCHHHHHHHHHCC	11.84	28787133
551 (in isoform 2)	Ubiquitination	-	9.06	-
584	Phosphorylation	VAGVLKLYFRSLEPP HHHHHHHHHHCCCCC	8.72	-
587	Phosphorylation	VLKLYFRSLEPPLFP HHHHHHHCCCCCCCC	27.47	-
686	O-linked_Glycosylation	RVNQLVQTLIVQPDR HHHHHHHHHCCCCCC	15.80	28657654
706	Phosphorylation	TSLPGPVYEKCMAPP CCCCCCHHCCCCCCC	16.89	-
746	Phosphorylation	PAQEDDLEGVVEAVA CCCCCCHHHHHHHHH	57.86	27642862
766	Phosphorylation	GRTAQELSFRRGDVL CCCHHHHCCCCCCEE	18.89	30108239
781	Phosphorylation	RLHERASSDWWRGEH EEEHHHCCCCCCCCC	35.94	28857561
798	Acetylation	MRGLIPHKYITLPAG CCCEECCCEEEECCC	32.75	25953088
798	Ubiquitination	MRGLIPHKYITLPAG CCCEECCCEEEECCC	32.75	-
817	Phosphorylation	VVGAGLQTAGESGSS EECCCCCCCCCCCCC	41.49	28464451
821	Phosphorylation	GLQTAGESGSSPEGL CCCCCCCCCCCCCCC	42.95	30108239
823	Phosphorylation	QTAGESGSSPEGLLA CCCCCCCCCCCCCCH	52.58	30108239
824	Phosphorylation	TAGESGSSPEGLLAS CCCCCCCCCCCCCHH	30.42	30108239
831	Phosphorylation	SPEGLLASELVHRPE CCCCCCHHHHCCCCC	31.28	30108239
838 (in isoform 2)	Ubiquitination	-	53.62	-
841	Phosphorylation	VHRPEPCTSPEAMGP CCCCCCCCCHHHCCC	59.02	30108239
842	Phosphorylation	HRPEPCTSPEAMGPS CCCCCCCCHHHCCCC	27.71	27422710
849	Phosphorylation	SPEAMGPSGHRRRCL CHHHCCCCCCCCCCC	41.31	30108239
860	Phosphorylation	RRCLVPASPEQHVEV CCCCCCCCHHHHHHH	23.93	23401153
863	Phosphorylation	LVPASPEQHVEVDKA CCCCCHHHHHHHHHH	49.68	27251275
886	Phosphorylation	FKELLGKTSVRQGLG HHHHHCCCCCCCCCC	30.56	23186163
887	Phosphorylation	KELLGKTSVRQGLGP HHHHCCCCCCCCCCC	20.84	23186163
896	Phosphorylation	RQGLGPASTTSPSPG CCCCCCCCCCCCCCC	35.04	22115753
897	Phosphorylation	QGLGPASTTSPSPGP CCCCCCCCCCCCCCC	33.14	29255136
898	Phosphorylation	GLGPASTTSPSPGPR CCCCCCCCCCCCCCC	35.82	29255136
899	Phosphorylation	LGPASTTSPSPGPRS CCCCCCCCCCCCCCC	24.38	23401153
900	Phosphorylation	GPASTTSPSPGPRSP CCCCCCCCCCCCCCC	42.31	24719451
901	Phosphorylation	PASTTSPSPGPRSPK CCCCCCCCCCCCCCC	42.11	29255136
906	O-linked_Glycosylation	SPSPGPRSPKAPPSS CCCCCCCCCCCCCCH	33.61	28657654
906	Phosphorylation	SPSPGPRSPKAPPSS CCCCCCCCCCCCCCH	33.61	23401153
912	Phosphorylation	RSPKAPPSSRLGRNK CCCCCCCCHHCCCCC	27.34	23403867
913	O-linked_Glycosylation	SPKAPPSSRLGRNKG CCCCCCCHHCCCCCC	36.98	28657654
913	Phosphorylation	SPKAPPSSRLGRNKG CCCCCCCHHCCCCCC	36.98	23403867
922	Phosphorylation	LGRNKGFSRGPGAPA CCCCCCCCCCCCCCC	45.02	26074081
930	Phosphorylation	RGPGAPASPSASHPQ CCCCCCCCCCCCCCC	20.52	23401153
932	Phosphorylation	PGAPASPSASHPQGL CCCCCCCCCCCCCCC	39.65	29255136
934	Phosphorylation	APASPSASHPQGLDT CCCCCCCCCCCCCCC	39.78	29255136
938	Phosphorylation	PSASHPQGLDTTPKP CCCCCCCCCCCCCCC	29.94	27251275
939	Phosphorylation	SASHPQGLDTTPKPH CCCCCCCCCCCCCCC	4.44	24719451
941	Phosphorylation	SHPQGLDTTPKPH-- CCCCCCCCCCCCC--	50.47	23401153
942	Phosphorylation	HPQGLDTTPKPH--- CCCCCCCCCCCC---	29.05	26055452
946	Phosphorylation	LDTTPKPH------- CCCCCCCC-------	57.34	24719451
970	Phosphorylation	------------------------------- -------------------------------		24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of RHG04_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RHG04_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RHG04_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
PCBP1_HUMAN	PCBP1	physical	26344197
RHG04_HUMAN	ARHGAP4	physical	26702831

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RHG04_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Tyrosine phosphorylated Par3 regulates epithelial tight junctionassembly promoted by EGFR signaling."; Wang Y., Du D., Fang L., Yang G., Zhang C., Zeng R., Ullrich A.,Lottspeich F., Chen Z.; EMBO J. 25:5058-5070(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-171, AND MASSSPECTROMETRY.	17053785 [Abstract]