dbPTM

NFAT5_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NFAT5_HUMAN
UniProt AC	O94916
Protein Name	Nuclear factor of activated T-cells 5
Gene Name	NFAT5
Organism	Homo sapiens (Human).
Sequence Length	1531
Subcellular Localization	Nucleus . Cytoplasm . Nuclear distribution increases under hypertonic conditions.
Protein Description	Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Mediates the transcriptional response to hypertonicity. [PubMed: 10051678 Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17]
Protein Sequence	MPSDFISLLSADLDLESPKSLYSRESVYDLLPKELQLPPSRETSVASMSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MPSDFISLLS -----CCHHHHHHHH	57.94	23663014
7	Phosphorylation	-MPSDFISLLSADLD -CCHHHHHHHHCCCC	24.21	23663014
10	Phosphorylation	SDFISLLSADLDLES HHHHHHHHCCCCCCC	25.65	23663014
17	Phosphorylation	SADLDLESPKSLYSR HCCCCCCCCCCCCCH	44.95	23663014
17 (in isoform 3)	Phosphorylation	-	44.95	20068231
17 (in isoform 4)	Phosphorylation	-	44.95	20068231
17 (in isoform 5)	Phosphorylation	-	44.95	20068231
20	Phosphorylation	LDLESPKSLYSRESV CCCCCCCCCCCHHHH	35.60	23663014
22	Phosphorylation	LESPKSLYSRESVYD CCCCCCCCCHHHHHH	16.87	23663014
23	Phosphorylation	ESPKSLYSRESVYDL CCCCCCCCHHHHHHH	34.56	24719451
26	Phosphorylation	KSLYSRESVYDLLPK CCCCCHHHHHHHCCH	25.52	23663014
28	Phosphorylation	LYSRESVYDLLPKEL CCCHHHHHHHCCHHH	15.34	23663014
44 (in isoform 3)	Phosphorylation	-	15.78	28796482
44 (in isoform 4)	Phosphorylation	-	15.78	28796482
44 (in isoform 5)	Phosphorylation	-	15.78	28796482
46	Phosphorylation	PSRETSVASMSQTSG CCCCCCCCCCCCCCC	10.17	-
46 (in isoform 3)	Phosphorylation	-	10.17	28796482
46 (in isoform 4)	Phosphorylation	-	10.17	28796482
46 (in isoform 5)	Phosphorylation	-	10.17	28796482
69	Phosphorylation	AVVAADASSAPSSSS CEEEECCCCCCCCCC	27.81	20068231
103	Sulfoxidation	SVTDSKAMQVESCSS ECCCCCCCCEEECHH	5.43	21406390
107	Phosphorylation	SKAMQVESCSSAVGV CCCCCEEECHHCCCC	22.05	25627689
109	Phosphorylation	AMQVESCSSAVGVSN CCCEEECHHCCCCCC	30.52	25627689
110	Phosphorylation	MQVESCSSAVGVSNR CCEEECHHCCCCCCC	31.16	25262027
115	Phosphorylation	CSSAVGVSNRGVSEK CHHCCCCCCCCCCHH	18.19	27251275
120	Phosphorylation	GVSNRGVSEKQLTSN CCCCCCCCHHHCCCC	41.73	21209322
122	Acetylation	SNRGVSEKQLTSNTV CCCCCCHHHCCCCCC	43.00	25953088
122	Ubiquitination	SNRGVSEKQLTSNTV CCCCCCHHHCCCCCC	43.00	-
125	Phosphorylation	GVSEKQLTSNTVQQH CCCHHHCCCCCCCCC	19.89	23927012
125 (in isoform 5)	Phosphorylation	-	19.89	24719451
126	Phosphorylation	VSEKQLTSNTVQQHP CCHHHCCCCCCCCCC	38.81	20068231
127 (in isoform 5)	Phosphorylation	-	38.75	27251275
128	Phosphorylation	EKQLTSNTVQQHPST HHHCCCCCCCCCCCC	21.32	22199227
134	Phosphorylation	NTVQQHPSTPKRHTV CCCCCCCCCCCCCEE	56.04	30266825
135	Phosphorylation	TVQQHPSTPKRHTVL CCCCCCCCCCCCEEE	36.96	30266825
137	Acetylation	QQHPSTPKRHTVLYI CCCCCCCCCCEEEEE	57.83	26051181
137	Ubiquitination	QQHPSTPKRHTVLYI CCCCCCCCCCEEEEE	57.83	-
140	Phosphorylation	PSTPKRHTVLYISPP CCCCCCCEEEEECCC	19.08	30266825
143	Phosphorylation	PKRHTVLYISPPPED CCCCEEEEECCCHHH	8.60	30266825
145	Phosphorylation	RHTVLYISPPPEDLL CCEEEEECCCHHHHC	20.14	30266825
153 (in isoform 5)	Phosphorylation	-	59.35	27251275
155	Phosphorylation	PEDLLDNSRMSCQDE HHHHCCCCCCCCCCC	29.09	29396449
158	Phosphorylation	LLDNSRMSCQDEGCG HCCCCCCCCCCCCCC	13.91	-
220	Phosphorylation	KRRDCEESNMDIFDA CHHHCCCCCCCCCCC	18.37	25849741
229	Phosphorylation	MDIFDADSAKAPHYV CCCCCCCCCCCCCEE	33.13	25849741
235	Phosphorylation	DSAKAPHYVLSQLTT CCCCCCCEEEECCCC	11.30	25159151
238	Phosphorylation	KAPHYVLSQLTTDNK CCCCEEEECCCCCCC	17.07	25159151
238 (in isoform 5)	Phosphorylation	-	17.07	27251275
241	Phosphorylation	HYVLSQLTTDNKGNS CEEEECCCCCCCCCC	25.19	20873877
242	Phosphorylation	YVLSQLTTDNKGNSK EEEECCCCCCCCCCC	46.28	23312004
247 (in isoform 5)	Phosphorylation	-	41.78	27251275
248	Phosphorylation	TTDNKGNSKAGNGTL CCCCCCCCCCCCCCC	32.33	20873877
256 (in isoform 5)	Phosphorylation	-	58.41	27251275
259 (in isoform 5)	Phosphorylation	-	70.76	27251275
266	Phosphorylation	KGTGVKKSPMLCGQY CCCCCCCCCCCCCCC	15.15	25159151
266 (in isoform 5)	Phosphorylation	-	15.15	27251275
276	Sumoylation	LCGQYPVKSEGKELK CCCCCCCCCCCEEEE	37.98	-
276	Sumoylation	LCGQYPVKSEGKELK CCCCCCCCCCCEEEE	37.98	-
282 (in isoform 2)	Acetylation	-	5.38	-
283	Sumoylation	KSEGKELKIVVQPET CCCCEEEEEEECCCC	34.25	-
283	Sumoylation	KSEGKELKIVVQPET CCCCEEEEEEECCCC	34.25	-
298	Phosphorylation	QHRARYLTEGSRGSV HHHHHHCCCCCCCCC	29.39	22210691
301	Phosphorylation	ARYLTEGSRGSVKDR HHHCCCCCCCCCCCC	27.51	22210691
339	Acetylation	GNDSGRVKPHGFYQA ECCCCCCCCCEEEEC	29.90	26051181
339	Malonylation	GNDSGRVKPHGFYQA ECCCCCCCCCEEEEC	29.90	26320211
425	Phosphorylation	NIMRKDGSTLTLQTP EEEECCCCEEEEECC	30.31	20068231
426	Phosphorylation	IMRKDGSTLTLQTPS EEECCCCEEEEECCC	29.32	20068231
428	Phosphorylation	RKDGSTLTLQTPSSP ECCCCEEEEECCCCC	19.53	25850435
431	Phosphorylation	GSTLTLQTPSSPILC CCEEEEECCCCCEEE	28.40	25850435
433	Phosphorylation	TLTLQTPSSPILCTQ EEEEECCCCCEEECC	52.73	25850435
434	Phosphorylation	LTLQTPSSPILCTQP EEEECCCCCEEECCC	19.70	29496963
439	Phosphorylation	PSSPILCTQPAGVPE CCCCEEECCCCCCHH	33.86	20068231
450	Ubiquitination	GVPEILKKSLHSCSV CCHHHHHHHHHCCCC	55.60	-
451	Phosphorylation	VPEILKKSLHSCSVK CHHHHHHHHHCCCCC	29.72	20068231
451 (in isoform 5)	Phosphorylation	-	29.72	24719451
452 (in isoform 5)	Phosphorylation	-	4.78	27251275
468	Ubiquitination	EEVFLIGKNFLKGTK EEEEEEECCCCCCCE	37.22	-
556	Sumoylation	GALNVNVKKEISSPA CCCCCCCCCCCCCCC	39.21	-
556	Sumoylation	GALNVNVKKEISSPA CCCCCCCCCCCCCCC	39.21	25114211
560	Phosphorylation	VNVKKEISSPARPCS CCCCCCCCCCCCCCC	31.21	29255136
561	Phosphorylation	NVKKEISSPARPCSF CCCCCCCCCCCCCCH	28.39	29255136
567	Phosphorylation	SSPARPCSFEEAMKA CCCCCCCCHHHHHHH	38.07	29255136
573	Acetylation	CSFEEAMKAMKTTGC CCHHHHHHHHHHHCC	52.99	25953088
576	Ubiquitination	EEAMKAMKTTGCNLD HHHHHHHHHHCCCCC	48.04	-
577 (in isoform 4)	Phosphorylation	-	18.48	25159151
578	Phosphorylation	AMKAMKTTGCNLDKV HHHHHHHHCCCCCCC	33.93	-
578 (in isoform 4)	Phosphorylation	-	33.93	25159151
579 (in isoform 5)	Phosphorylation	-	11.98	27251275
594	Phosphorylation	IIPNALMTPLIPSSM CCCCHHCCCCCCHHH	18.78	-
599	Phosphorylation	LMTPLIPSSMIKSED HCCCCCCHHHCCCCC	26.08	-
600	Phosphorylation	MTPLIPSSMIKSEDV CCCCCCHHHCCCCCC	21.12	28857561
603	Sumoylation	LIPSSMIKSEDVTPM CCCHHHCCCCCCCCC	38.87	28112733
608	Phosphorylation	MIKSEDVTPMEVTAE HCCCCCCCCCEEEEE	29.28	21815630
610	Sulfoxidation	KSEDVTPMEVTAEKR CCCCCCCCEEEEEHH	4.77	21406390
616	Acetylation	PMEVTAEKRSSTIFK CCEEEEEHHCCCCEE	55.93	25953088
618	Phosphorylation	EVTAEKRSSTIFKTT EEEEEHHCCCCEECC	42.79	29396449
619	Phosphorylation	VTAEKRSSTIFKTTK EEEEHHCCCCEECCC	29.56	28555341
620	Phosphorylation	TAEKRSSTIFKTTKS EEEHHCCCCEECCCC	31.51	29396449
624	Phosphorylation	RSSTIFKTTKSVGST HCCCCEECCCCCCCH	28.80	26699800
625	Phosphorylation	SSTIFKTTKSVGSTQ CCCCEECCCCCCCHH	23.16	26699800
627	Phosphorylation	TIFKTTKSVGSTQQT CCEECCCCCCCHHHH	29.62	26699800
630	Phosphorylation	KTTKSVGSTQQTLEN ECCCCCCCHHHHHHH	22.38	26699800
631	Phosphorylation	TTKSVGSTQQTLENI CCCCCCCHHHHHHHH	20.83	26699800
634	Phosphorylation	SVGSTQQTLENISNI CCCCHHHHHHHHHHH	26.65	26699800
646	Phosphorylation	SNIAGNGSFSSPSSS HHHCCCCCCCCCCCC	26.42	22199227
648	Phosphorylation	IAGNGSFSSPSSSHL HCCCCCCCCCCCCCC	42.93	29496963
649	Phosphorylation	AGNGSFSSPSSSHLP CCCCCCCCCCCCCCC	27.13	29496963
651	Phosphorylation	NGSFSSPSSSHLPSE CCCCCCCCCCCCCCC	46.55	22199227
652	Phosphorylation	GSFSSPSSSHLPSEN CCCCCCCCCCCCCCC	25.76	22199227
653	Phosphorylation	SFSSPSSSHLPSENE CCCCCCCCCCCCCCH	33.12	22199227
657	Phosphorylation	PSSSHLPSENEKQQQ CCCCCCCCCCHHHHC	60.46	22199227
680	Phosphorylation	ETLTTIQTQDISQPG CCCCEEEECCCCCCC	25.10	28348404
684	Phosphorylation	TIQTQDISQPGTFPA EEEECCCCCCCCCCC	38.32	28348404
688	Phosphorylation	QDISQPGTFPAVSAS CCCCCCCCCCCCCCC	32.88	28348404
701	Phosphorylation	ASSQLPNSDALLQQA CCCCCCCHHHHHHHH	23.48	28348404
702 (in isoform 5)	Phosphorylation	-	48.12	27251275
744	Phosphorylation	ASQQQQQSPLQEQAQ HHHHHHCCHHHHHHH	24.20	22468782
1197	Phosphorylation	HIQTPMLSQEQAQPP CCCCCCCCHHHCCCC	25.97	15173573
1247	Phosphorylation	AMQSNSPSQEQQQQQ EECCCCHHHHHHHHH	46.28	15173573
1364	Phosphorylation	QIQLVQGSPSSQEQQ CEEEECCCCCCHHHE	12.43	26074081
1366	Phosphorylation	QLVQGSPSSQEQQVT EEECCCCCCHHHEEE	46.48	26074081
1367	Phosphorylation	LVQGSPSSQEQQVTL EECCCCCCHHHEEEE	40.91	15173573

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
135	T	Phosphorylation	Kinase	CDK5	Q00535	Uniprot
143	Y	Phosphorylation	Kinase	ABL1	P00519	GPS
158	S	Phosphorylation	Kinase	CSNK1A1	P48729	GPS
158	S	Phosphorylation	Kinase	CSNK1A1L	Q8N752	GPS
1197	S	Phosphorylation	Kinase	ATM	Q13315	PSP
1247	S	Phosphorylation	Kinase	ATM	Q13315	PSP
1367	S	Phosphorylation	Kinase	ATM	Q13315	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	MDM2	Q00987	PMID:16338656

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
135	T	Phosphorylation		21209322
Phosphorylated at Thr-135 by CDK5 in response to osmotic stress; this phosphorylation mediates its rapid nuclear localization.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NFAT5_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NFAT5_HUMAN	NFAT5	physical	11485737
FOS_HUMAN	FOS	physical	18056707
JUN_HUMAN	JUN	physical	18056707
KAPCA_HUMAN	PRKACA	physical	12482947

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NFAT5_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"High NaCl-induced activation of CDK5 increases phosphorylation of theosmoprotective transcription factor TonEBP/OREBP at threonine 135,which contributes to its rapid nuclear localization."; Gallazzini M., Heussler G.E., Kunin M., Izumi Y., Burg M.B.,Ferraris J.D.; Mol. Biol. Cell 22:703-714(2011). Cited for: PHOSPHORYLATION AT SER-120; SER-134; THR-135 AND SER-155, ANDMUTAGENESIS OF SER-120; SER-134; THR-135 AND SER-155.	21209322 [Abstract]

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