PFKAM_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PFKAM_HUMAN
• UniProt AC: P08237
• Protein Name: ATP-dependent 6-phosphofructokinase, muscle type {ECO:0000255|HAMAP-Rule:MF_03184}
• Gene Name: PFKM
• Organism: Homo sapiens (Human).
• Sequence Length: 780
• Subcellular Localization: Cytoplasm .
• Protein Description: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis..
• Protein Sequence: MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MTHEEHHAA ------CCHHHHHHH		-
2	Phosphorylation	------MTHEEHHAA ------CCHHHHHHH		17287340
10	Acetylation	HEEHHAAKTLGIGKA HHHHHHHHHCCCCCE		20167786
10	Ubiquitination	HEEHHAAKTLGIGKA HHHHHHHHHCCCCCE		-
16	Ubiquitination	AKTLGIGKAIAVLTS HHHCCCCCEEEEEEC		-
16 (in isoform 2)	Ubiquitination	-		21890473
16 (in isoform 1)	Ubiquitination	-		21890473
23 (in isoform 3)	Phosphorylation	-		27251275
27 (in isoform 3)	Phosphorylation	-		30622161
30	Sulfoxidation	SGGDAQGMNAAVRAV CCCCHHHHHHHHHHH		28183972
32 (in isoform 3)	Phosphorylation	-		30622161
33 (in isoform 3)	Phosphorylation	-		24114839
34 (in isoform 3)	Phosphorylation	-		30622161
35 (in isoform 3)	Phosphorylation	-		30622161
50 (in isoform 3)	Phosphorylation	-		30622161
53 (in isoform 3)	Phosphorylation	-		30622161
59 (in isoform 3)	Phosphorylation	-		20068231
62 (in isoform 3)	Phosphorylation	-		20068231
69	Phosphorylation	GDHIKEATWESVSMM CHHHHHHHHHHHHHH		23532336
72	Phosphorylation	IKEATWESVSMMLQL HHHHHHHHHHHHHHC		20068231
74	Phosphorylation	EATWESVSMMLQLGG HHHHHHHHHHHHCCC		20068231
82	Phosphorylation	MMLQLGGTVIGSARC HHHHCCCEEEEECCC		20068231
86	Phosphorylation	LGGTVIGSARCKDFR CCCEEEEECCCHHHH		20068231
103	Phosphorylation	EGRLRAAYNLVKRGI HCHHHHHHHHHHCCC		19764811
107 (in isoform 2)	Ubiquitination	-		-
130	Phosphorylation	TGADTFRSEWSDLLS CCHHHHHHHHHHHHH		21712546
133	Phosphorylation	DTFRSEWSDLLSDLQ HHHHHHHHHHHHHHH		21712546
137	Phosphorylation	SEWSDLLSDLQKAGK HHHHHHHHHHHHHCC		21712546
141	Ubiquitination	DLLSDLQKAGKITDE HHHHHHHHHCCCCCH		-
141 (in isoform 2)	Ubiquitination	-		21890473
141 (in isoform 1)	Ubiquitination	-		21890473
144	Ubiquitination	SDLQKAGKITDEEAT HHHHHHCCCCCHHHH		-
144 (in isoform 2)	Ubiquitination	-		21890473
144 (in isoform 1)	Ubiquitination	-		21890473
146	Phosphorylation	LQKAGKITDEEATKS HHHHCCCCCHHHHHH		23532336
151	Phosphorylation	KITDEEATKSSYLNI CCCCHHHHHHHHHHH		23532336
186	Sulfoxidation	DSALHRIMEIVDAIT HHHHHHHHHHHHHHH		28183972
198	Phosphorylation	AITTTAQSHQRTFVL HHHHHCHHHHHHHHH		21712546
202	Phosphorylation	TAQSHQRTFVLEVMG HCHHHHHHHHHHHCH		21406692
208	Sulfoxidation	RTFVLEVMGRHCGYL HHHHHHHCHHHHCHH		30846556
272	Ubiquitination	GAIDKNGKPITSEDI CCCCCCCCCCCHHHH		-
272 (in isoform 2)	Ubiquitination	-		21890473
272 (in isoform 1)	Ubiquitination	-		21890473
280	Ubiquitination	PITSEDIKNLVVKRL CCCHHHHHHHHHHHC		-
280 (in isoform 2)	Ubiquitination	-		21890473
301	Methylation	TVLGHVQRGGTPSAF EEEECHHHCCCHHHH		-
304	Phosphorylation	GHVQRGGTPSAFDRI ECHHHCCCHHHHHHH		28857561
306	Phosphorylation	VQRGGTPSAFDRILG HHHCCCHHHHHHHHH		22673903
310	Methylation	GTPSAFDRILGSRMG CCHHHHHHHHHHHHH		115487121
315	Methylation	FDRILGSRMGVEAVM HHHHHHHHHHHHHHH		115487131
328	Phosphorylation	VMALLEGTPDTPACV HHHHHHCCCCCCEEE		-
329 (in isoform 2)	Ubiquitination	-		21890473
331	Phosphorylation	LLEGTPDTPACVVSL HHHCCCCCCEEEEEC		-
335 (in isoform 2)	Ubiquitination	-		21890473
341 (in isoform 2)	Ubiquitination	-		21890473
349	Sulfoxidation	QAVRLPLMECVQVTK CEEEEEHHHHHHHHH		21406390
359	Phosphorylation	VQVTKDVTKAMDEKK HHHHHHHHHHHCHHH		27251275
360	Acetylation	QVTKDVTKAMDEKKF HHHHHHHHHHCHHHH		27452117
360	Ubiquitination	QVTKDVTKAMDEKKF HHHHHHHHHHCHHHH		-
360 (in isoform 1)	Ubiquitination	-		21890473
366	Acetylation	TKAMDEKKFDEALKL HHHHCHHHHHHHHHH		7367639
366	Ubiquitination	TKAMDEKKFDEALKL HHHHCHHHHHHHHHH		-
366 (in isoform 1)	Ubiquitination	-		21890473
372	Methylation	KKFDEALKLRGRSFM HHHHHHHHHCCCCHH		14496225
372	Ubiquitination	KKFDEALKLRGRSFM HHHHHHHHHCCCCHH		-
372	Malonylation	KKFDEALKLRGRSFM HHHHHHHHHCCCCHH		26320211
372 (in isoform 1)	Ubiquitination	-		21890473
377	Phosphorylation	ALKLRGRSFMNNWEV HHHHCCCCHHCCHHH		25159151
396	Phosphorylation	AHVRPPVSKSGSHTV HCCCCCCCCCCCCEE		26437602
397	Ubiquitination	HVRPPVSKSGSHTVA CCCCCCCCCCCCEEE		-
398	Phosphorylation	VRPPVSKSGSHTVAV CCCCCCCCCCCEEEE		19764811
400	Phosphorylation	PPVSKSGSHTVAVMN CCCCCCCCCEEEEEE		19764811
402	Phosphorylation	VSKSGSHTVAVMNVG CCCCCCCEEEEEECC		19764811
414 (in isoform 2)	Ubiquitination	-		21890473
435 (in isoform 2)	Ubiquitination	-		21890473
445	Ubiquitination	DGFEGLAKGQIEEAG CCCCHHHCCCCEECC		-
445 (in isoform 1)	Ubiquitination	-		21890473
445 (in isoform 2)	Ubiquitination	-		21890473
454	Phosphorylation	QIEEAGWSYVGGWTG CCEECCCEEECCCCC		26437602
466	Ubiquitination	WTGQGGSKLGTKRTL CCCCCCCCCCCCCCC		-
466 (in isoform 1)	Ubiquitination	-		21890473
472	Phosphorylation	SKLGTKRTLPKKSFE CCCCCCCCCCCCHHH		26437602
476	Ubiquitination	TKRTLPKKSFEQISA CCCCCCCCHHHHHHH		-
476	Malonylation	TKRTLPKKSFEQISA CCCCCCCCHHHHHHH		26320211
476 (in isoform 1)	Ubiquitination	-		21890473
477	Phosphorylation	KRTLPKKSFEQISAN CCCCCCCHHHHHHHC		26437602
502	Phosphorylation	IIGGFEAYTGGLELM EECCEEEECCCHHCC		18083107
530	O-linked_Glycosylation	VVIPATVSNNVPGSD EEEECCCCCCCCCCC		UniProtKB CARBOHYD
557	Ubiquitination	CTTCDRIKQSAAGTK HHHHHHHHHCCCCCC		-
557	Other	CTTCDRIKQSAAGTK HHHHHHHHHCCCCCC		29775581
563	Phosphorylation	IKQSAAGTKRRVFII HHHCCCCCCCEEEEE		25367160
564	Acetylation	KQSAAGTKRRVFIIE HHCCCCCCCEEEEEE		30591229
572	Phosphorylation	RRVFIIETMGGYCGY CEEEEEEECCCHHHH		22210691
576	Phosphorylation	IIETMGGYCGYLATM EEEECCCHHHHHHHH		22210691
584 (in isoform 2)	Ubiquitination	-		21890473
601	Phosphorylation	YIFEEPFTIRDLQAN EECCCCCCHHHHHHH		24719451
615	Ubiquitination	NVEHLVQKMKTTVKR HHHHHHHHHHHHHHH		-
615	Acetylation	NVEHLVQKMKTTVKR HHHHHHHHHHHHHHH		26051181
615 (in isoform 1)	Ubiquitination	-		21890473
625 (in isoform 2)	Ubiquitination	-		21890473
647 (in isoform 2)	Ubiquitination	-		21890473
651 (in isoform 2)	Ubiquitination	-		21890473
656	Ubiquitination	KGIFDSRKNVLGHMQ CCCCCCCCHHHHCHH		-
656	Malonylation	KGIFDSRKNVLGHMQ CCCCCCCCHHHHCHH		26320211
656 (in isoform 1)	Ubiquitination	-		21890473
667	Phosphorylation	GHMQQGGSPTPFDRN HCHHCCCCCCCCCHH		25159151
669	Phosphorylation	MQQGGSPTPFDRNFA HHCCCCCCCCCHHHH		21712546
673	Methylation	GSPTPFDRNFATKMG CCCCCCCHHHHHHHH		115487141
677	Phosphorylation	PFDRNFATKMGAKAM CCCHHHHHHHHHHHH		24719451
678	Ubiquitination	FDRNFATKMGAKAMN CCHHHHHHHHHHHHH		-
678	Acetylation	FDRNFATKMGAKAMN CCHHHHHHHHHHHHH		26051181
678 (in isoform 1)	Ubiquitination	-		21890473
682	Ubiquitination	FATKMGAKAMNWMSG HHHHHHHHHHHHHCC		-
682 (in isoform 1)	Ubiquitination	-		21890473
696 (in isoform 2)	Ubiquitination	-		21890473
707 (in isoform 2)	Ubiquitination	-		21890473
713	Sulfoxidation	DSGCVLGMRKRALVF CCCCCCCCCCEEEEE		21406390
727	Acetylation	FQPVAELKDQTDFEH EEEHHHHCCCCCCCC		23236377
727	Ubiquitination	FQPVAELKDQTDFEH EEEHHHHCCCCCCCC		21906983
727 (in isoform 1)	Ubiquitination	-		21890473
730	Phosphorylation	VAELKDQTDFEHRIP HHHHCCCCCCCCCCC		23663014
738	Acetylation	DFEHRIPKEQWWLKL CCCCCCCHHHHHHHH		20167786
738	Ubiquitination	DFEHRIPKEQWWLKL CCCCCCCHHHHHHHH		21906983
738 (in isoform 1)	Ubiquitination	-		21890473
744	Ubiquitination	PKEQWWLKLRPILKI CHHHHHHHHHHHHHH		-
750	Ubiquitination	LKLRPILKILAKYEI HHHHHHHHHHHHCCC		-
754	Ubiquitination	PILKILAKYEIDLDT HHHHHHHHCCCCCCC		-
755	Phosphorylation	ILKILAKYEIDLDTS HHHHHHHCCCCCCCC		26437602
761	Phosphorylation	KYEIDLDTSDHAHLE HCCCCCCCCCCHHHH		29214152
762	Phosphorylation	YEIDLDTSDHAHLEH CCCCCCCCCCHHHHH		29214152
771	Phosphorylation	HAHLEHITRKRSGEA CHHHHHHHCCCCCCC		26437602
773	Ubiquitination	HLEHITRKRSGEAAV HHHHHHCCCCCCCCC		-
775	Phosphorylation	EHITRKRSGEAAV-- HHHHCCCCCCCCC--		1825608
809	Ubiquitination	------------------------------------ ------------------------------------		21890473

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
74	S	Phosphorylation	Kinase	ULK1	O75385	PSP
762	S	Phosphorylation	Kinase	ULK1	O75385	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PFKAM_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PFKAM_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CAV3_HUMAN	CAV3	physical	9252390
PFKAL_HUMAN	PFKL	physical	22939629
PFKAP_HUMAN	PFKP	physical	22939629
PCBP1_HUMAN	PCBP1	physical	22863883
PFKAP_HUMAN	PFKP	physical	22863883
PFKAM_HUMAN	PFKM	physical	25416956
SIAH1_HUMAN	SIAH1	physical	25416956
ACPH_HUMAN	APEH	physical	26344197
ARFP1_HUMAN	ARFIP1	physical	26344197
CGL_HUMAN	CTH	physical	26344197
EF1A1_HUMAN	EEF1A1	physical	26344197
MCFD2_HUMAN	MCFD2	physical	26344197
PICAL_HUMAN	PICALM	physical	26344197
PRRC1_HUMAN	PRRC1	physical	26344197
PSMG1_HUMAN	PSMG1	physical	26344197
SUMO3_HUMAN	SUMO3	physical	26344197
XPO6_HUMAN	XPO6	physical	26344197
OTU1_HUMAN	YOD1	physical	26344197
TEAD4_HUMAN	TEAD4	physical	25796446
TEAD1_HUMAN	TEAD1	physical	25796446

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 232800: Glycogen storage disease 7 (GSD7)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.