ARFP1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ARFP1_HUMAN
• UniProt AC: P53367
• Protein Name: Arfaptin-1
• Gene Name: ARFIP1
• Organism: Homo sapiens (Human).
• Sequence Length: 373
• Protein Description: Putative target protein of ADP-ribosylation factor..
• Protein Sequence: MAQESPKNSAAEIPVTSNGEVDDSREHSFNRDLKHSLPSGLGLSETQITSHGFDNTKEGVIEAGAFQGSPAPPLPSVMSPSRVAASRLAQQGSDLIVPAGGQRTQTKSGPVILADEIKNPAMEKLELVRKWSLNTYKCTRQIISEKLGRGSRTVDLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLELHEEFGYNADTQKLLAKNGETLLGAINFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRDANTLPKIEQSQHLFQAHKEKYDKMRNDVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQFHIKLKTPGVDAPSWLEEQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAQESPKNS ------CCCCCCCCC	23.11	21406692
5 (in isoform 2)	Phosphorylation	-	29.15	-
5	Phosphorylation	---MAQESPKNSAAE ---CCCCCCCCCCCC	29.15	23401153
9	Phosphorylation	AQESPKNSAAEIPVT CCCCCCCCCCCCCCC	34.67	22617229
16	Phosphorylation	SAAEIPVTSNGEVDD CCCCCCCCCCCCCCC	15.89	23401153
17	Phosphorylation	AAEIPVTSNGEVDDS CCCCCCCCCCCCCCC	42.97	23663014
24 (in isoform 2)	Phosphorylation	-	34.80	-
24	Phosphorylation	SNGEVDDSREHSFNR CCCCCCCCHHHCCCH	34.80	25159151
28 (in isoform 2)	Phosphorylation	-	21.33	-
28	Phosphorylation	VDDSREHSFNRDLKH CCCCHHHCCCHHHHH	21.33	25159151
34	Ubiquitination	HSFNRDLKHSLPSGL HCCCHHHHHCCCCCC	35.12	-
36	Phosphorylation	FNRDLKHSLPSGLGL CCHHHHHCCCCCCCC	40.15	22617229
36 (in isoform 2)	Phosphorylation	-	40.15	-
39	Phosphorylation	DLKHSLPSGLGLSET HHHHCCCCCCCCCCH	52.52	23401153
39 (in isoform 2)	Phosphorylation	-	52.52	-
44	Phosphorylation	LPSGLGLSETQITSH CCCCCCCCCHHHEEC	36.48	22617229
46	Phosphorylation	SGLGLSETQITSHGF CCCCCCCHHHEECCC	23.15	25693802
49	Phosphorylation	GLSETQITSHGFDNT CCCCHHHEECCCCCC	12.59	23403867
50	Phosphorylation	LSETQITSHGFDNTK CCCHHHEECCCCCCC	24.20	23403867
56	Phosphorylation	TSHGFDNTKEGVIEA EECCCCCCCCCEEEE	32.11	23403867
69	Phosphorylation	EAGAFQGSPAPPLPS EECCCCCCCCCCCCC	13.70	29255136
76	Phosphorylation	SPAPPLPSVMSPSRV CCCCCCCCCCCHHHH	37.83	29255136
76 (in isoform 2)	Phosphorylation	-	37.83	25159151
78	Sulfoxidation	APPLPSVMSPSRVAA CCCCCCCCCHHHHHH	5.98	30846556
79	Phosphorylation	PPLPSVMSPSRVAAS CCCCCCCCHHHHHHH	19.98	29255136
81	Phosphorylation	LPSVMSPSRVAASRL CCCCCCHHHHHHHHH	31.89	29255136
86 (in isoform 2)	Ubiquitination	-	20.35	21890473
86	Phosphorylation	SPSRVAASRLAQQGS CHHHHHHHHHHHCCC	20.35	23090842
93	Phosphorylation	SRLAQQGSDLIVPAG HHHHHCCCCEEECCC	25.55	25693802
100 (in isoform 2)	Phosphorylation	-	27.05	-
104	Phosphorylation	VPAGGQRTQTKSGPV ECCCCCCCCCCCCCE	32.47	28555341
107	Ubiquitination	GGQRTQTKSGPVILA CCCCCCCCCCCEEEE	43.53	-
108	Phosphorylation	GQRTQTKSGPVILAD CCCCCCCCCCEEEEH	52.28	21815630
118	Ubiquitination	VILADEIKNPAMEKL EEEEHHCCCHHHHHH	55.63	21906983
118 (in isoform 1)	Ubiquitination	-	55.63	21890473
124	Ubiquitination	IKNPAMEKLELVRKW CCCHHHHHHHHHHHH	33.89	-
124	2-Hydroxyisobutyrylation	IKNPAMEKLELVRKW CCCHHHHHHHHHHHH	33.89	-
132	Phosphorylation	LELVRKWSLNTYKCT HHHHHHHCCCHHHHH	18.12	22167270
135	Phosphorylation	VRKWSLNTYKCTRQI HHHHCCCHHHHHHHH	29.65	22167270
136	Phosphorylation	RKWSLNTYKCTRQII HHHCCCHHHHHHHHH	11.73	23403867
144	Phosphorylation	KCTRQIISEKLGRGS HHHHHHHHHHHCCCC	30.24	24719451
146	Acetylation	TRQIISEKLGRGSRT HHHHHHHHHCCCCCE	49.83	25953088
146	2-Hydroxyisobutyrylation	TRQIISEKLGRGSRT HHHHHHHHHCCCCCE	49.83	-
146	Ubiquitination	TRQIISEKLGRGSRT HHHHHHHHHCCCCCE	49.83	-
172	Phosphorylation	LRDNKKKYENILKLA HHCCHHHHHHHHHHH	24.08	22210691
173	Ubiquitination	RDNKKKYENILKLAQ HCCHHHHHHHHHHHH	46.83	21890473
180	Ubiquitination	ENILKLAQTLSTQLF HHHHHHHHHHHHHHH	54.10	-
192	Phosphorylation	QLFQMVHTQRQLGDA HHHHHHHHHHHHHHH	17.65	22210691
221 (in isoform 2)	Phosphorylation	-	34.64	-
222	Ubiquitination	GYNADTQKLLAKNGE CCCHHHHHHHHHCCC	47.78	-
249	Phosphorylation	VNTLVNKTIEDTLMT HHHHHCHHHHHHHHH	25.13	22210691
253	Phosphorylation	VNKTIEDTLMTVKQY HCHHHHHHHHHHHHH	12.97	20068231
255	Sulfoxidation	KTIEDTLMTVKQYES HHHHHHHHHHHHHHH	4.37	21406390
256	Phosphorylation	TIEDTLMTVKQYESA HHHHHHHHHHHHHHC	27.88	22210691
258	Ubiquitination	EDTLMTVKQYESARI HHHHHHHHHHHHCCC	37.69	-
272	Phosphorylation	IEYDAYRTDLEELNL CEECEECCCHHHCCC	32.06	19060867
289	Ubiquitination	RDANTLPKIEQSQHL CCCCCCHHHHHHHHH	63.09	-
293	Phosphorylation	TLPKIEQSQHLFQAH CCHHHHHHHHHHHHH	13.53	30622161
301	Ubiquitination	QHLFQAHKEKYDKMR HHHHHHHHHHHHHHH	59.94	-
304	Phosphorylation	FQAHKEKYDKMRNDV HHHHHHHHHHHHCCH	23.14	-
316	Ubiquitination	NDVSVKLKFLEENKV CCHHHHHHHHHHCCC	42.03	-
321 (in isoform 2)	Ubiquitination	-	45.07	21890473
321	Ubiquitination	KLKFLEENKVKVLHN HHHHHHHCCCCEEHH	45.07	21890473
322	Ubiquitination	LKFLEENKVKVLHNQ HHHHHHCCCCEEHHH	46.87	-
322	2-Hydroxyisobutyrylation	LKFLEENKVKVLHNQ HHHHHHCCCCEEHHH	46.87	-
353 (in isoform 1)	Ubiquitination	-	55.06	21890473
353	Ubiquitination	KQLEQTLKQFHIKLK HHHHHHHHHHCHHCC	55.06	21890473
360	Ubiquitination	KQFHIKLKTPGVDAP HHHCHHCCCCCCCCC	47.90	-
361	Phosphorylation	QFHIKLKTPGVDAPS HHCHHCCCCCCCCCH	35.39	28355574
368	Phosphorylation	TPGVDAPSWLEEQ-- CCCCCCCHHHHCC--	46.11	26552605

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
100	S	Phosphorylation	Kinase	PRKD1	Q15139	GPS
100	S	Phosphorylation	Kinase	PRKD2	Q9BZL6	PSP
132	S	Phosphorylation	Kinase	PRKD1	Q15139	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ARFP1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ARFP1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ARF3_HUMAN	ARF3	physical	10413101
DNM1L_HUMAN	DNM1L	physical	22863883
IDE_HUMAN	IDE	physical	22863883
LC7L2_HUMAN	LUC7L2	physical	22863883
ANM3_HUMAN	PRMT3	physical	22863883
PSMG3_HUMAN	PSMG3	physical	22863883
SC24A_HUMAN	SEC24A	physical	22863883
GLYM_HUMAN	SHMT2	physical	22863883
STAT3_HUMAN	STAT3	physical	22863883
AACS_HUMAN	AACS	physical	26344197
AK1A1_HUMAN	AKR1A1	physical	26344197
ATOX1_HUMAN	ATOX1	physical	26344197
ECH1_HUMAN	ECH1	physical	26344197
FLNB_HUMAN	FLNB	physical	26344197
LYPA1_HUMAN	LYPLA1	physical	26344197
PA1B2_HUMAN	PAFAH1B2	physical	26344197
PDCD6_HUMAN	PDCD6	physical	26344197
PGM2L_HUMAN	PGM2L1	physical	26344197
DHPR_HUMAN	QDPR	physical	26344197
STIP1_HUMAN	STIP1	physical	26344197
TBCA_HUMAN	TBCA	physical	26344197

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, AND MASSSPECTROMETRY.
PubMed: 19413330

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-69; SER-79 AND SER-132,AND MASS SPECTROMETRY.
PubMed: 18669648

"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions.";
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.;
Sci. Signal. 2:RA46-RA46(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-272 AND THR-361, ANDMASS SPECTROMETRY.
PubMed: 19690332