PICAL_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PICAL_HUMAN
• UniProt AC: Q13492
• Protein Name: Phosphatidylinositol-binding clathrin assembly protein
• Gene Name: PICALM
• Organism: Homo sapiens (Human).
• Sequence Length: 652
• Subcellular Localization: Membrane, clathrin-coated pit . Golgi apparatus . Cytoplasmic vesicle, clathrin-coated vesicle . Nucleus . Colocalized with clathrin in the Golgi area (PubMed:10436022). Interaction with PIMREG may target PICALM to the nucleus in some cells (PubMed:1
• Protein Description: Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction..
• Protein Sequence: MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDYLIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNTLFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMNTEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINLLEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRLKELAKKPHTSLTTAASPVSTSAGGIMTAPAIDIFSTPSSSNSTSKLPNDLLDLQQPTFHPSVHPMSTASQVASTWGDPFSATVDAVDDAIPSLNPFLTKSSGDVHLSISSDVSTFTTRTPTHEMFVGFTPSPVAQPHPSAGLNVDFESVFGNKSTNVIVDSGGFDELGGLLKPTVASQNQNLPVAKLPPSKLVSDDLDSSLANLVGNLGIGNGTTKNDVNWSQPGEKKLTGGSNWQPKVAPTTAWNAATMAPPVMAYPATTPTGMIGYGIPPQMGSVPVMTQPTLIYSQPVMRPPNPFGPVSGAQIQFM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSGQSLTDR ------CCCCCHHHH	45.61	21406692
2	Phosphorylation	------MSGQSLTDR ------CCCCCHHHH	45.61	25159151
2 (in isoform 2)	Phosphorylation	-	45.61	21406692
2 (in isoform 3)	Acetylation	-	45.61	-
5	Phosphorylation	---MSGQSLTDRITA ---CCCCCHHHHHHH	36.44	22199227
7	Phosphorylation	-MSGQSLTDRITAAQ -CCCCCHHHHHHHHH	28.60	22199227
9	Methylation	SGQSLTDRITAAQHS CCCCHHHHHHHHHHC	23.95	115487525
11	Phosphorylation	QSLTDRITAAQHSVT CCHHHHHHHHHHCCC	19.48	25159151
16	Phosphorylation	RITAAQHSVTGSAVS HHHHHHHCCCCCHHC	14.86	23401153
18	Phosphorylation	TAAQHSVTGSAVSKT HHHHHCCCCCHHCHH	28.56	25159151
20	Phosphorylation	AQHSVTGSAVSKTVC HHHCCCCCHHCHHHH	19.01	25159151
23	Phosphorylation	SVTGSAVSKTVCKAT CCCCCHHCHHHHHHH	23.48	22199227
24	Acetylation	VTGSAVSKTVCKATT CCCCHHCHHHHHHHH	38.08	25953088
24	Malonylation	VTGSAVSKTVCKATT CCCCHHCHHHHHHHH	38.08	26320211
24	Ubiquitination	VTGSAVSKTVCKATT CCCCHHCHHHHHHHH	38.08	-
25	Phosphorylation	TGSAVSKTVCKATTH CCCHHCHHHHHHHHC	24.51	22199227
28	Acetylation	AVSKTVCKATTHEIM HHCHHHHHHHHCHHH	44.42	11793985
28	Malonylation	AVSKTVCKATTHEIM HHCHHHHHHHHCHHH	44.42	26320211
35	Sulfoxidation	KATTHEIMGPKKKHL HHHHCHHHCCCHHHH	7.38	30846556
67	Phosphorylation	ADSLFERTTNSSWVV HHHHHHHCCCCCEEE	24.11	20860994
82	Phosphorylation	VFKSLITTHHLMVYG EEEHHHCCCEEEECC	10.58	-
88	Phosphorylation	TTHHLMVYGNERFIQ CCCEEEECCCHHHHH	10.56	-
102	Phosphorylation	QYLASRNTLFNLSNF HHHHHCCCHHHHHHH	31.45	24076635
107	Phosphorylation	RNTLFNLSNFLDKSG CCCHHHHHHHHCCCC	27.00	20068231
107 (in isoform 3)	Phosphorylation	-	27.00	-
112	Ubiquitination	NLSNFLDKSGLQGYD HHHHHHCCCCCCCCC	48.87	-
112 (in isoform 2)	Ubiquitination	-	48.87	-
134	Ubiquitination	YSRYLNEKAVSYRQV HHHHHCHHCCCHHEE	53.68	-
134 (in isoform 2)	Ubiquitination	-	53.68	-
137	Phosphorylation	YLNEKAVSYRQVAFD HHCHHCCCHHEEECC	21.32	24732914
138	Phosphorylation	LNEKAVSYRQVAFDF HCHHCCCHHEEECCC	9.85	21712546
146	Phosphorylation	RQVAFDFTKVKRGAD HEEECCCCCCCCCCC	36.45	24732914
158	Phosphorylation	GADGVMRTMNTEKLL CCCCCHHCCCHHHHH	9.18	29978859
161	Phosphorylation	GVMRTMNTEKLLKTV CCHHCCCHHHHHHHC	24.55	29978859
163	2-Hydroxyisobutyrylation	MRTMNTEKLLKTVPI HHCCCHHHHHHHCHH	58.02	-
163	Ubiquitination	MRTMNTEKLLKTVPI HHCCCHHHHHHHCHH	58.02	-
167	Phosphorylation	NTEKLLKTVPIIQNQ CHHHHHHHCHHHHHH	31.33	29978859
222	Phosphorylation	IINLLEKYFDMKKNQ HHHHHHHHHHHCHHH	8.74	25367160
231	Ubiquitination	DMKKNQCKEGLDIYK HHCHHHHHHHHHHHH	45.08	-
237	Phosphorylation	CKEGLDIYKKFLTRM HHHHHHHHHHHHHHH	14.06	-
238	Sumoylation	KEGLDIYKKFLTRMT HHHHHHHHHHHHHHH	37.07	-
238	Sumoylation	KEGLDIYKKFLTRMT HHHHHHHHHHHHHHH	37.07	25218447
248	Phosphorylation	LTRMTRISEFLKVAE HHHHHHHHHHHHHHH	21.00	26846344
252	Ubiquitination	TRISEFLKVAEQVGI HHHHHHHHHHHHHCC	44.73	21906983
252 (in isoform 1)	Ubiquitination	-	44.73	21890473
252 (in isoform 2)	Ubiquitination	-	44.73	21890473
252 (in isoform 3)	Ubiquitination	-	44.73	21890473
288	Acetylation	HLASLEGKKIKDSTA HHHHHCCCCCCCCHH	41.78	26051181
288	Ubiquitination	HLASLEGKKIKDSTA HHHHHCCCCCCCCHH	41.78	-
291	Ubiquitination	SLEGKKIKDSTAASR HHCCCCCCCCHHHHH	55.28	-
293	O-linked_Glycosylation	EGKKIKDSTAASRAT CCCCCCCCHHHHHHH	18.23	30059200
293	Phosphorylation	EGKKIKDSTAASRAT CCCCCCCCHHHHHHH	18.23	28348404
294	O-linked_Glycosylation	GKKIKDSTAASRATT CCCCCCCHHHHHHHH	36.05	30059200
297	O-linked_Glycosylation	IKDSTAASRATTLSN CCCCHHHHHHHHHHH	21.71	30059200
297	Phosphorylation	IKDSTAASRATTLSN CCCCHHHHHHHHHHH	21.71	-
300	Phosphorylation	STAASRATTLSNAVS CHHHHHHHHHHHHHH	27.45	26846344
301	Phosphorylation	TAASRATTLSNAVSS HHHHHHHHHHHHHHH	27.49	26846344
301 (in isoform 3)	Phosphorylation	-	27.49	-
303	Phosphorylation	ASRATTLSNAVSSLA HHHHHHHHHHHHHHH	22.20	26846344
303 (in isoform 2)	Phosphorylation	-	22.20	21406692
303 (in isoform 3)	Phosphorylation	-	22.20	-
307	Phosphorylation	TTLSNAVSSLASTGL HHHHHHHHHHHHCCC	19.75	21712546
307 (in isoform 3)	Phosphorylation	-	19.75	-
308	Phosphorylation	TLSNAVSSLASTGLS HHHHHHHHHHHCCCC	22.74	21712546
308 (in isoform 3)	Phosphorylation	-	22.74	-
311	Phosphorylation	NAVSSLASTGLSLTK HHHHHHHHCCCCCCC	28.44	26657352
312	Phosphorylation	AVSSLASTGLSLTKV HHHHHHHCCCCCCCC	36.21	25693802
315	Phosphorylation	SLASTGLSLTKVDER HHHHCCCCCCCCCHH	35.02	25218447
317	Phosphorylation	ASTGLSLTKVDEREK HHCCCCCCCCCHHHH	26.16	30108239
318	Ubiquitination	STGLSLTKVDEREKQ HCCCCCCCCCHHHHH	53.64	21890473
318	Ubiquitination	STGLSLTKVDEREKQ HCCCCCCCCCHHHHH	53.64	21890473
318 (in isoform 1)	Ubiquitination	-	53.64	21890473
318 (in isoform 2)	Ubiquitination	-	53.64	21890473
318 (in isoform 3)	Ubiquitination	-	53.64	21890473
318	Ubiquitination	STGLSLTKVDEREKQ HCCCCCCCCCHHHHH	53.64	21890473
324	Ubiquitination	TKVDEREKQAALEEE CCCCHHHHHHHHHHH	52.00	21906983
324 (in isoform 1)	Ubiquitination	-	52.00	21890473
324 (in isoform 2)	Ubiquitination	-	52.00	21890473
324 (in isoform 3)	Ubiquitination	-	52.00	21890473
336	Ubiquitination	EEEQARLKALKEQRL HHHHHHHHHHHHHHH	46.23	-
352	O-linked_Glycosylation	ELAKKPHTSLTTAAS HHHCCCCCCCCCCCC	33.68	30059200
352	Phosphorylation	ELAKKPHTSLTTAAS HHHCCCCCCCCCCCC	33.68	30576142
353	O-linked_Glycosylation	LAKKPHTSLTTAASP HHCCCCCCCCCCCCC	22.23	30059200
353	Phosphorylation	LAKKPHTSLTTAASP HHCCCCCCCCCCCCC	22.23	30108239
355	O-linked_Glycosylation	KKPHTSLTTAASPVS CCCCCCCCCCCCCCC	17.73	30059200
355	Phosphorylation	KKPHTSLTTAASPVS CCCCCCCCCCCCCCC	17.73	30576142
356	O-linked_Glycosylation	KPHTSLTTAASPVST CCCCCCCCCCCCCCC	26.06	30059200
356	Phosphorylation	KPHTSLTTAASPVST CCCCCCCCCCCCCCC	26.06	30108239
359	O-linked_Glycosylation	TSLTTAASPVSTSAG CCCCCCCCCCCCCCC	24.30	30059200
359	Phosphorylation	TSLTTAASPVSTSAG CCCCCCCCCCCCCCC	24.30	25159151
359 (in isoform 3)	Phosphorylation	-	24.30	-
362	Phosphorylation	TTAASPVSTSAGGIM CCCCCCCCCCCCCCC	21.74	30576142
363	Phosphorylation	TAASPVSTSAGGIMT CCCCCCCCCCCCCCC	23.88	30576142
363 (in isoform 3)	Phosphorylation	-	23.88	-
364	Phosphorylation	AASPVSTSAGGIMTA CCCCCCCCCCCCCCC	19.87	30108239
370	Phosphorylation	TSAGGIMTAPAIDIF CCCCCCCCCCEEEEE	27.80	29496963
378	Phosphorylation	APAIDIFSTPSSSNS CCEEEEECCCCCCCC	39.48	20068231
379	Phosphorylation	PAIDIFSTPSSSNST CEEEEECCCCCCCCC	19.21	30576142
379 (in isoform 3)	Phosphorylation	-	19.21	-
381	Phosphorylation	IDIFSTPSSSNSTSK EEEECCCCCCCCCCC	46.62	20068231
382	Phosphorylation	DIFSTPSSSNSTSKL EEECCCCCCCCCCCC	34.44	26074081
383	Phosphorylation	IFSTPSSSNSTSKLP EECCCCCCCCCCCCC	38.85	26074081
385	Phosphorylation	STPSSSNSTSKLPND CCCCCCCCCCCCCCC	35.69	26074081
386	Phosphorylation	TPSSSNSTSKLPNDL CCCCCCCCCCCCCCH	33.85	23186163
387	Phosphorylation	PSSSNSTSKLPNDLL CCCCCCCCCCCCCHH	31.83	23186163
441	Phosphorylation	PSLNPFLTKSSGDVH HHCCCCCCCCCCCEE	29.29	26074081
443	O-linked_Glycosylation	LNPFLTKSSGDVHLS CCCCCCCCCCCEEEE	34.03	30059200
443	Phosphorylation	LNPFLTKSSGDVHLS CCCCCCCCCCCEEEE	34.03	26074081
444	Phosphorylation	NPFLTKSSGDVHLSI CCCCCCCCCCEEEEE	40.34	26074081
450	Phosphorylation	SSGDVHLSISSDVST CCCCEEEEEECCCCC	12.92	25159151
452	O-linked_Glycosylation	GDVHLSISSDVSTFT CCEEEEEECCCCCEE	19.67	30059200
452	Phosphorylation	GDVHLSISSDVSTFT CCEEEEEECCCCCEE	19.67	27251275
453	O-linked_Glycosylation	DVHLSISSDVSTFTT CEEEEEECCCCCEEC	40.12	30059200
453	Phosphorylation	DVHLSISSDVSTFTT CEEEEEECCCCCEEC	40.12	27251275
462	Phosphorylation	VSTFTTRTPTHEMFV CCCEECCCCCCCCCC	30.37	20068231
464	Phosphorylation	TFTTRTPTHEMFVGF CEECCCCCCCCCCCC	29.83	20068231
472	Phosphorylation	HEMFVGFTPSPVAQP CCCCCCCCCCCCCCC	19.54	29523821
474	Phosphorylation	MFVGFTPSPVAQPHP CCCCCCCCCCCCCCC	29.02	20068231
482	Phosphorylation	PVAQPHPSAGLNVDF CCCCCCCCCCCCCCH	31.75	20068231
515	Ubiquitination	DELGGLLKPTVASQN CCCCCCCCCCCCCCC	43.62	-
517	O-linked_Glycosylation	LGGLLKPTVASQNQN CCCCCCCCCCCCCCC	27.41	30059200
520 (in isoform 3)	Ubiquitination	-	25.90	21890473
533	Phosphorylation	PVAKLPPSKLVSDDL CCCCCCHHHCCCCCC	36.36	22210691
534 (in isoform 2)	Ubiquitination	-	59.69	-
537	Phosphorylation	LPPSKLVSDDLDSSL CCHHHCCCCCCCHHH	35.52	22199227
542	Phosphorylation	LVSDDLDSSLANLVG CCCCCCCHHHHHHHH	34.25	22199227
543	Phosphorylation	VSDDLDSSLANLVGN CCCCCCHHHHHHHHC	31.03	22199227
559	Ubiquitination	GIGNGTTKNDVNWSQ CCCCCCCCCCCCCCC	51.49	-
565	O-linked_Glycosylation	TKNDVNWSQPGEKKL CCCCCCCCCCCCCCC	22.69	30059200
565	Phosphorylation	TKNDVNWSQPGEKKL CCCCCCCCCCCCCCC	22.69	22210691
570	Acetylation	NWSQPGEKKLTGGSN CCCCCCCCCCCCCCC	60.02	25953088
570	Ubiquitination	NWSQPGEKKLTGGSN CCCCCCCCCCCCCCC	60.02	2190698
570 (in isoform 1)	Ubiquitination	-	60.02	21890473
570 (in isoform 2)	Ubiquitination	-	60.02	21890473
571	Ubiquitination	WSQPGEKKLTGGSNW CCCCCCCCCCCCCCC	47.15	-
573	O-linked_Glycosylation	QPGEKKLTGGSNWQP CCCCCCCCCCCCCCC	48.66	30059200
573	Phosphorylation	QPGEKKLTGGSNWQP CCCCCCCCCCCCCCC	48.66	22777824
576	O-linked_Glycosylation	EKKLTGGSNWQPKVA CCCCCCCCCCCCCCC	35.92	30059200
576	Phosphorylation	EKKLTGGSNWQPKVA CCCCCCCCCCCCCCC	35.92	25159151
585	O-linked_Glycosylation	WQPKVAPTTAWNAAT CCCCCCCCHHHCHHH	21.29	30059200
585 (in isoform 2)	Phosphorylation	-	21.29	22210691
586	O-linked_Glycosylation	QPKVAPTTAWNAATM CCCCCCCHHHCHHHC	28.72	30059200
586 (in isoform 2)	Phosphorylation	-	28.72	22210691
592 (in isoform 2)	Phosphorylation	-	16.08	22210691
599 (in isoform 2)	Phosphorylation	-	13.85	21406692
604 (in isoform 2)	Phosphorylation	-	22.43	21406692
607 (in isoform 2)	Phosphorylation	-	13.60	21406692
610 (in isoform 2)	Phosphorylation	-	13.01	22210691
611 (in isoform 2)	Phosphorylation	-	10.85	21406692
636	Dimethylation	IYSQPVMRPPNPFGP EEECCCCCCCCCCCC	43.70	-
636	Methylation	IYSQPVMRPPNPFGP EEECCCCCCCCCCCC	43.70	52717269
645	Phosphorylation	PNPFGPVSGAQIQFM CCCCCCCCCCEEEEC	31.38	20068231

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	SMURF1	Q9HCE7	PMID:20804422

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PICAL_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PICAL_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PLCG1_HUMAN	PLCG1	physical	10926122
CLH1_HUMAN	CLTC	physical	10436022
SC24D_HUMAN	SEC24D	physical	22939629
SC24C_HUMAN	SEC24C	physical	22939629
VATE1_HUMAN	ATP6V1E1	physical	22939629
SHLB2_HUMAN	SH3GLB2	physical	22939629
VATF_HUMAN	ATP6V1F	physical	22939629
CN166_HUMAN	C14orf166	physical	26344197
SYCC_HUMAN	CARS	physical	26344197
CPPED_HUMAN	CPPED1	physical	26344197
EF1A1_HUMAN	EEF1A1	physical	26344197
HIP1R_HUMAN	HIP1R	physical	26344197
HNRPD_HUMAN	HNRNPD	physical	26344197
ILVBL_HUMAN	ILVBL	physical	26344197
ITB1_HUMAN	ITGB1	physical	26344197
LPP_HUMAN	LPP	physical	26344197
PAPOA_HUMAN	PAPOLA	physical	26344197
PAXI_HUMAN	PXN	physical	26344197
STXB1_HUMAN	STXBP1	physical	26344197
TRI25_HUMAN	TRIM25	physical	26344197
UN13D_HUMAN	UNC13D	physical	26344197
AP2B1_HUMAN	AP2B1	physical	26496610
AP2M1_HUMAN	AP2M1	physical	26496610
CLCA_HUMAN	CLTA	physical	26496610
CLCB_HUMAN	CLTB	physical	26496610
CLH1_HUMAN	CLTC	physical	26496610
DAB2_HUMAN	DAB2	physical	26496610
EPS15_HUMAN	EPS15	physical	26496610
GAK_HUMAN	GAK	physical	26496610
MYO6_HUMAN	MYO6	physical	26496610
P3C2A_HUMAN	PIK3C2A	physical	26496610
SEC13_HUMAN	SEC13	physical	26496610
CLH2_HUMAN	CLTCL1	physical	26496610
HIP1R_HUMAN	HIP1R	physical	26496610
EPN4_HUMAN	CLINT1	physical	26496610
SC16A_HUMAN	SEC16A	physical	26496610
TNR6A_HUMAN	TNRC6A	physical	26496610
GTSE1_HUMAN	GTSE1	physical	26496610
BMP2K_HUMAN	BMP2K	physical	26496610
MICA3_HUMAN	MICAL3	physical	26496610
DEN1A_HUMAN	DENND1A	physical	26496610
WNK1_HUMAN	WNK1	physical	26496610
PRC2B_HUMAN	PRRC2B	physical	26496610
STON2_HUMAN	STON2	physical	26496610
FCHO2_HUMAN	FCHO2	physical	26496610
CLH2_HUMAN	CLTCL1	physical	28514442
BMP2K_HUMAN	BMP2K	physical	28514442
P3C2A_HUMAN	PIK3C2A	physical	28514442
GTSE1_HUMAN	GTSE1	physical	28514442
AP2A1_HUMAN	AP2A1	physical	28514442
RBP1_HUMAN	RALBP1	physical	28514442
EPN4_HUMAN	CLINT1	physical	28514442
EPS15_HUMAN	EPS15	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.