dbPTM

FCHO2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	FCHO2_HUMAN
UniProt AC	Q0JRZ9
Protein Name	F-BAR domain only protein 2
Gene Name	FCHO2
Organism	Homo sapiens (Human).
Sequence Length	810
Subcellular Localization	Membrane, clathrin-coated pit Peripheral membrane protein Cytoplasmic side. Associated with forming but not mature clathrin-coated vesicles. The recruitment to coated-pits precede the one of clathrin and the adaptor protein complex AP-2 (By similar
Protein Description	Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides (Pi(4,5) biphosphate) like the plasma membrane. Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP-2-dependent endocytosis of the transferrin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor..
Protein Sequence	MVMAYFVENFWGEKNSGFDVLYHNMKHGQISTKELADFVRERATIEEAYSRSMTKLAKSASNYSQLGTFAPVWDVFKTSTEKLANCHLDLVRKLQELIKEVQKYGEEQVKSHKKTKEEVAGTLEAVQTIQSITQALQKSKENYNAKCVEQERLKKEGATQREIEKAAVKSKKATDTYKLYVEKYALAKADFEQKMTETAQKFQDIEETHLIHIKEIIGSLSNAIKEIHLQIGQVHEEFINNMANTTVESLIQKFAESKGTGKERPGLIEFEECDTASAVEGIKPRKRKTFALPGIIKKEKDAESVECPDADSLNIPDVDEEGYSIKPETNQNDTKENHFYSSSDSDSEDEEPKKYRIEIKPMHPNNSHHTMASLDELKVSIGNITLSPAISRHSPVQMNRNLSNEELTKSKPSAPPNEKGTSDLLAWDPLFGPSLDSSSSSSLTSSSSARPTTPLSVGTIVPPPRPASRPKLTSGKLSGINEIPRPFSPPVTSNTSPPPAAPLARAESSSSISSSASLSAANTPTVGVSRGPSPVSLGNQDTLPVAVALTESVNAYFKGADPTKCIVKITGDMTMSFPSGIIKVFTSNPTPAVLCFRVKNISRLEQILPNAQLVFSDPSQCDSNTKDFWMNMQAVTVYLKKLSEQNPAASYYNVDVLKYQVSSNGIQSTPLNLATYWKCSASTTDLRVDYKYNPEAMVAPSVLSNIQVVVPVDGGVTNMQSLPPAIWNAEQMKAFWKLSSISEKSENGGSGSLRAKFDLSEGPSKPTTLAVQFLSEGSTLSGVDFELVGTGYRLSLIKKRFATGRYLADC

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
22	Phosphorylation	NSGFDVLYHNMKHGQ CCCCCEEEECCCCCC	7.32	21253578
26	Malonylation	DVLYHNMKHGQISTK CEEEECCCCCCCCHH	50.31	26320211
44	O-linked_Glycosylation	DFVRERATIEEAYSR HHHHHHHHHHHHHHH	34.83	30379171
44	Phosphorylation	DFVRERATIEEAYSR HHHHHHHHHHHHHHH	34.83	18452278
55	Ubiquitination	AYSRSMTKLAKSASN HHHHHHHHHHHHHCC	37.49	22817900
58	Ubiquitination	RSMTKLAKSASNYSQ HHHHHHHHHHCCCHH	57.92	21890473
58	Ubiquitination	RSMTKLAKSASNYSQ HHHHHHHHHHCCCHH	57.92	22817900
58 (in isoform 1)	Ubiquitination	-	57.92	21890473
58 (in isoform 2)	Ubiquitination	-	57.92	21890473
59	Phosphorylation	SMTKLAKSASNYSQL HHHHHHHHHCCCHHC	31.18	28270605
61	Phosphorylation	TKLAKSASNYSQLGT HHHHHHHCCCHHCCC	43.31	28270605
63	Phosphorylation	LAKSASNYSQLGTFA HHHHHCCCHHCCCCC	8.69	28270605
64	Phosphorylation	AKSASNYSQLGTFAP HHHHCCCHHCCCCCC	24.15	28270605
68	Phosphorylation	SNYSQLGTFAPVWDV CCCHHCCCCCCHHHH	25.80	28270605
77	Ubiquitination	APVWDVFKTSTEKLA CCHHHHHHHCHHHHH	41.22	32015554
82	Acetylation	VFKTSTEKLANCHLD HHHHCHHHHHHCCHH	53.65	26051181
99	2-Hydroxyisobutyrylation	RKLQELIKEVQKYGE HHHHHHHHHHHHHCH	65.54	-
99	Acetylation	RKLQELIKEVQKYGE HHHHHHHHHHHHHCH	65.54	178226245
99	Ubiquitination	RKLQELIKEVQKYGE HHHHHHHHHHHHHCH	65.54	29967540
103	Acetylation	ELIKEVQKYGEEQVK HHHHHHHHHCHHHHH	61.47	7711281
103	Ubiquitination	ELIKEVQKYGEEQVK HHHHHHHHHCHHHHH	61.47	33845483
165	Ubiquitination	ATQREIEKAAVKSKK CCHHHHHHHHHHCCC	47.51	-
174	Phosphorylation	AVKSKKATDTYKLYV HHHCCCCCCHHHHHH	37.44	29083192
176	Phosphorylation	KSKKATDTYKLYVEK HCCCCCCHHHHHHHH	20.43	29083192
177	Phosphorylation	SKKATDTYKLYVEKY CCCCCCHHHHHHHHH	11.21	29083192
180	Phosphorylation	ATDTYKLYVEKYALA CCCHHHHHHHHHHHH	11.47	29083192
188	Ubiquitination	VEKYALAKADFEQKM HHHHHHHHCHHHHHH	49.71	29967540
194	Ubiquitination	AKADFEQKMTETAQK HHCHHHHHHHHHHHH	40.36	32015554
223	Ubiquitination	IIGSLSNAIKEIHLQ HHHHHHHHHHHHHHH	15.74	22817900
225	Ubiquitination	GSLSNAIKEIHLQIG HHHHHHHHHHHHHHH	48.48	22817900
227	Ubiquitination	LSNAIKEIHLQIGQV HHHHHHHHHHHHHHH	3.14	21963094
229	Ubiquitination	NAIKEIHLQIGQVHE HHHHHHHHHHHHHHH	4.95	21963094
248	Ubiquitination	NMANTTVESLIQKFA HHCCHHHHHHHHHHH	37.42	23000965
250	Ubiquitination	ANTTVESLIQKFAES CCHHHHHHHHHHHHH	2.81	23000965
251	Ubiquitination	NTTVESLIQKFAESK CHHHHHHHHHHHHHC	6.11	23000965
253	Ubiquitination	TVESLIQKFAESKGT HHHHHHHHHHHHCCC	39.28	23000965
255	Ubiquitination	ESLIQKFAESKGTGK HHHHHHHHHHCCCCC	27.47	23000965
258	Ubiquitination	IQKFAESKGTGKERP HHHHHHHCCCCCCCC	52.58	22817900
262	Acetylation	AESKGTGKERPGLIE HHHCCCCCCCCCCEE	50.84	26051181
262	Ubiquitination	AESKGTGKERPGLIE HHHCCCCCCCCCCEE	50.84	21890473
264	Ubiquitination	SKGTGKERPGLIEFE HCCCCCCCCCCEEEE	33.79	21890473
264	Ubiquitination	SKGTGKERPGLIEFE HCCCCCCCCCCEEEE	33.79	21890473
283	Acetylation	ASAVEGIKPRKRKTF CHHCCCCCCCCCCCC	50.02	26051181
283	Ubiquitination	ASAVEGIKPRKRKTF CHHCCCCCCCCCCCC	50.02	23000965
286	Ubiquitination	VEGIKPRKRKTFALP CCCCCCCCCCCCCCC	67.77	23000965
288	Ubiquitination	GIKPRKRKTFALPGI CCCCCCCCCCCCCCC	51.54	23000965
289	Phosphorylation	IKPRKRKTFALPGII CCCCCCCCCCCCCCE	20.41	27422710
297	Sumoylation	FALPGIIKKEKDAES CCCCCCEECCCCCCC	53.62	28112733
297	Ubiquitination	FALPGIIKKEKDAES CCCCCCEECCCCCCC	53.62	21890473
297 (in isoform 1)	Ubiquitination	-	53.62	21890473
297 (in isoform 2)	Ubiquitination	-	53.62	21890473
304	Phosphorylation	KKEKDAESVECPDAD ECCCCCCCCCCCCHH	25.57	26657352
312	Phosphorylation	VECPDADSLNIPDVD CCCCCHHHCCCCCCC	25.65	25159151
323	Phosphorylation	PDVDEEGYSIKPETN CCCCCCCCCCCCCCC	15.53	20873877
324	Phosphorylation	DVDEEGYSIKPETNQ CCCCCCCCCCCCCCC	34.69	20873877
329	Phosphorylation	GYSIKPETNQNDTKE CCCCCCCCCCCCCCC	51.11	23927012
334	Phosphorylation	PETNQNDTKENHFYS CCCCCCCCCCCCCCC	48.05	23927012
340	Phosphorylation	DTKENHFYSSSDSDS CCCCCCCCCCCCCCC	10.49	24275569
341	Phosphorylation	TKENHFYSSSDSDSE CCCCCCCCCCCCCCC	23.56	25849741
342	Phosphorylation	KENHFYSSSDSDSED CCCCCCCCCCCCCCC	27.13	25849741
343	Phosphorylation	ENHFYSSSDSDSEDE CCCCCCCCCCCCCCC	34.88	25849741
345	Phosphorylation	HFYSSSDSDSEDEEP CCCCCCCCCCCCCCC	45.01	25849741
347	Phosphorylation	YSSSDSDSEDEEPKK CCCCCCCCCCCCCCE	51.39	25849741
354	Phosphorylation	SEDEEPKKYRIEIKP CCCCCCCEEEEEEEC	50.94	32142685
361	Phosphorylation	KYRIEIKPMHPNNSH EEEEEEECCCCCCCC	31.79	32142685
367	Phosphorylation	KPMHPNNSHHTMASL ECCCCCCCCCCCCCH	24.24	28348404
370	Phosphorylation	HPNNSHHTMASLDEL CCCCCCCCCCCHHHC	14.71	32645325
380	O-linked_Glycosylation	SLDELKVSIGNITLS CHHHCEEEECCEEEC	24.16	30379171
380	Phosphorylation	SLDELKVSIGNITLS CHHHCEEEECCEEEC	24.16	25159151
385	Phosphorylation	KVSIGNITLSPAISR EEEECCEEECCCHHH	25.78	30266825
387	Phosphorylation	SIGNITLSPAISRHS EECCEEECCCHHHCC	12.02	19664994
391	O-linked_Glycosylation	ITLSPAISRHSPVQM EEECCCHHHCCCCCC	26.50	30379171
391	Phosphorylation	ITLSPAISRHSPVQM EEECCCHHHCCCCCC	26.50	29255136
394	Phosphorylation	SPAISRHSPVQMNRN CCCHHHCCCCCCCCC	26.17	22167270
403	Phosphorylation	VQMNRNLSNEELTKS CCCCCCCCHHHHHCC	45.83	29255136
408	Phosphorylation	NLSNEELTKSKPSAP CCCHHHHHCCCCCCC	36.23	30266825
410	Phosphorylation	SNEELTKSKPSAPPN CHHHHHCCCCCCCCC	44.56	24275569
444	Phosphorylation	SSSSSSLTSSSSARP CCCCCCCCCCCCCCC	28.63	27251275
445	Phosphorylation	SSSSSLTSSSSARPT CCCCCCCCCCCCCCC	33.25	27251275
446	Phosphorylation	SSSSLTSSSSARPTT CCCCCCCCCCCCCCC	24.07	27251275
447	Phosphorylation	SSSLTSSSSARPTTP CCCCCCCCCCCCCCC	27.99	28348404
448	Phosphorylation	SSLTSSSSARPTTPL CCCCCCCCCCCCCCC	30.19	28348404
452	Phosphorylation	SSSSARPTTPLSVGT CCCCCCCCCCCCCEE	35.18	25850435
453	Phosphorylation	SSSARPTTPLSVGTI CCCCCCCCCCCCEEE	25.55	26657352
455	Phosphorylation	SARPTTPLSVGTIVP CCCCCCCCCCEEECC	6.43	32142685
456	Phosphorylation	ARPTTPLSVGTIVPP CCCCCCCCCEEECCC	21.50	20873877
459	Phosphorylation	TTPLSVGTIVPPPRP CCCCCCEEECCCCCC	19.35	20873877
460	Phosphorylation	TPLSVGTIVPPPRPA CCCCCEEECCCCCCC	3.44	32142685
468	Phosphorylation	VPPPRPASRPKLTSG CCCCCCCCCCCCCCC	52.37	26657352
473	Phosphorylation	PASRPKLTSGKLSGI CCCCCCCCCCCCCCC	41.37	21406692
474	Phosphorylation	ASRPKLTSGKLSGIN CCCCCCCCCCCCCCC	44.35	22496350
478	Phosphorylation	KLTSGKLSGINEIPR CCCCCCCCCCCCCCC	40.90	23927012
488	Phosphorylation	NEIPRPFSPPVTSNT CCCCCCCCCCCCCCC	30.86	29255136
492	Phosphorylation	RPFSPPVTSNTSPPP CCCCCCCCCCCCCCC	23.25	29255136
493	Phosphorylation	PFSPPVTSNTSPPPA CCCCCCCCCCCCCCC	38.04	29255136
495	Phosphorylation	SPPVTSNTSPPPAAP CCCCCCCCCCCCCCC	42.23	29255136
496	Phosphorylation	PPVTSNTSPPPAAPL CCCCCCCCCCCCCCC	39.39	25159151
508	Phosphorylation	APLARAESSSSISSS CCCCCCCCCCCCCCC	34.24	30278072
509	Phosphorylation	PLARAESSSSISSSA CCCCCCCCCCCCCCC	21.43	30278072
510	Phosphorylation	LARAESSSSISSSAS CCCCCCCCCCCCCCC	40.76	30278072
511	Phosphorylation	ARAESSSSISSSASL CCCCCCCCCCCCCCC	28.56	23911959
513	Phosphorylation	AESSSSISSSASLSA CCCCCCCCCCCCCCC	21.86	30278072
514	Phosphorylation	ESSSSISSSASLSAA CCCCCCCCCCCCCCC	28.40	30576142
515	Phosphorylation	SSSSISSSASLSAAN CCCCCCCCCCCCCCC	18.32	30278072
517	Phosphorylation	SSISSSASLSAANTP CCCCCCCCCCCCCCC	25.46	30278072
519	Phosphorylation	ISSSASLSAANTPTV CCCCCCCCCCCCCCE	23.88	30278072
523	Phosphorylation	ASLSAANTPTVGVSR CCCCCCCCCCEECCC	18.66	28857561
525	Phosphorylation	LSAANTPTVGVSRGP CCCCCCCCEECCCCC	28.05	23186163
529	Phosphorylation	NTPTVGVSRGPSPVS CCCCEECCCCCCCCC	26.12	23186163
533	Phosphorylation	VGVSRGPSPVSLGNQ EECCCCCCCCCCCCC	40.53	30278072
536	Phosphorylation	SRGPSPVSLGNQDTL CCCCCCCCCCCCCCC	33.66	30278072
542	Phosphorylation	VSLGNQDTLPVAVAL CCCCCCCCCCHHHHH	24.17	23663014
550	Phosphorylation	LPVAVALTESVNAYF CCHHHHHHHHHHHHH	19.24	25850435
552	Phosphorylation	VAVALTESVNAYFKG HHHHHHHHHHHHHCC	18.55	25850435
556	Phosphorylation	LTESVNAYFKGADPT HHHHHHHHHCCCCCC	10.71	26330541
608	Ubiquitination	ISRLEQILPNAQLVF HHHHHHHCCCCEEEE	2.40	29967540
616	Phosphorylation	PNAQLVFSDPSQCDS CCCEEEECCHHHCCC	40.92	28387310
641	Ubiquitination	AVTVYLKKLSEQNPA HHHHHHHHHHHCCCC	55.43	29967540
651	Phosphorylation	EQNPAASYYNVDVLK HCCCCHHEECEEEEE	8.37	-
676	Phosphorylation	TPLNLATYWKCSAST CCEEEECEEECCCCC	9.39	-
678	Acetylation	LNLATYWKCSASTTD EEEECEEECCCCCCC	14.11	7704225
682	Phosphorylation	TYWKCSASTTDLRVD CEEECCCCCCCCEEE	18.62	22798277
683	Phosphorylation	YWKCSASTTDLRVDY EEECCCCCCCCEEEC	24.85	28348404
684	Phosphorylation	WKCSASTTDLRVDYK EECCCCCCCCEEECC	30.46	24961811
711	Ubiquitination	SNIQVVVPVDGGVTN HCCEEEEECCCCCCC	13.58	32015554
739	Phosphorylation	MKAFWKLSSISEKSE HHHHHHHHHCCCCCC	23.15	27251275
740	Phosphorylation	KAFWKLSSISEKSEN HHHHHHHHCCCCCCC	40.34	27251275
744	Ubiquitination	KLSSISEKSENGGSG HHHHCCCCCCCCCCC	57.17	32015554
795	Phosphorylation	VGTGYRLSLIKKRFA ECCCEEHHHHHHHHC	21.03	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of FCHO2_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of FCHO2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of FCHO2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
EPS15_HUMAN	EPS15	physical	21762413
DAB2_HUMAN	DAB2	physical	22323290

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of FCHO2_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-403, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-488; SER-510 ANDSER-511, AND MASS SPECTROMETRY.	18669648 [Abstract]
"A probability-based approach for high-throughput proteinphosphorylation analysis and site localization."; Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.; Nat. Biotechnol. 24:1285-1292(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-387 AND SER-403, ANDMASS SPECTROMETRY.	16964243 [Abstract]