dbPTM

IBTK_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	IBTK_HUMAN
UniProt AC	Q9P2D0
Protein Name	Inhibitor of Bruton tyrosine kinase
Gene Name	IBTK
Organism	Homo sapiens (Human).
Sequence Length	1353
Subcellular Localization	Cytoplasm. Membrane Peripheral membrane protein. Translocates to the plasma membrane upon IgM stimulation. Isoform 2: Nucleus.
Protein Description	Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription..
Protein Sequence	MSSPMPDCTSKCRSLKHALDVLSVVTKGSENQIKAFLSSHCYNAATIKDVFGRNALHLVSSCGKKGVLDWLIQKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKNTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGIYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLVPRLVEGLNGHNCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQIQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGCLLDPNGEKCVTAPRQVSALHHKDIALSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGHMEYKVDPEHLKENGGQKICILAMDGAGRVFCWRSVNSSLKQCRWAYPRQVFISDIALNRNEILFVTQDGEGFRGRWFEEKRKSSEKKEILSNLHNSSSDVSYVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLREADEMDSIHDVTFQVGNRLFPAHKYILAVHSDFFQKLFLSDGNTSEFTDIYQKDEDSAGCHLFVVEKVHPDMFEYLLQFIYTDTCDFLTHGFKPRIHLNKNPEEYQGTLNSHLNKVNFHEDDNQKSAFEVYKSNQAQTVSERQKSKPKSCKKGKNIREDDPVRMLQTVAKKFDFSNLSSRLDGVRFENEKINVIAKNTGNKLKLSQKKCSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASSCAALEMPIHSDILKVILDYLYTDEAVVIKESQNVDFICSVLVVADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYSAKQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSEFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDIFLKEEINMEQNHSETMFKKAKTKAKKKPRKRSDSSGGYNLSDIIQSPSSTGLLKSGKTNSVESLPELLTSDSEGSYAGVGSPRDLQSPDFTTGFHSDKIEAKVKPYVNGTSPVYSREDLKPWEKSPILKISAPQPIPSNRIDTTSSASWVAGSFSPVSPPVVDLRTIMEIEESRQKCGATPKSHLGKTVSHGVKLSQKQRKMIALTTKENNSGMNSMETVLFTPSKAPKPVNAWASSLHSVSSKSFRDFLLEEKKSVTSHSSGDHVKKVSFKGIENSQAPKIVRCSTHGTPGPEGNHISDLPLLDSPNPWLSSSVTAPSMVAPVTFASIVEEELQQEAALIRSREKPLALIQIEEHAIQDLLVFYEAFGNPEEFVIVERTPQGPLAVPMWNKHGC

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSSPMPDCT ------CCCCCCCCH	55.22	24719451
3	Phosphorylation	-----MSSPMPDCTS -----CCCCCCCCHH	36.76	25627689
11	Ubiquitination	PMPDCTSKCRSLKHA CCCCCHHHHHHHHHH	18.60	-
27	Ubiquitination	DVLSVVTKGSENQIK HHHHHHCCCCHHHHH	49.15	-
64	Ubiquitination	HLVSSCGKKGVLDWL HHHHHCCCCCHHHHH	51.16	-
81	Ubiquitination	KGVDLLVKDKESGWT CCCCEEEECCCCCCC	64.15	-
96	Phosphorylation	ALHRSIFYGHIDCVW HHHHHHHCCHHHHHH	13.21	-
104	Phosphorylation	GHIDCVWSLLKHGVS CHHHHHHHHHHCCCE	11.92	22817900
113	Phosphorylation	LKHGVSLYIQDKEGL HHCCCEEEEECCCCC	6.77	-
128	Ubiquitination	SALDLVMKDRPTHVV CHHHHHCCCCCCEEE	43.01	-
137	Ubiquitination	RPTHVVFKNTDPTDV CCCEEEECCCCCCCC	47.53	-
285	Phosphorylation	AKYLKGRTIIGVAAG HHHHCCCEEEEEEEC	26.14	-
296	Phosphorylation	VAAGRFHTVLWTREA EEECCCEEEEEECHH	18.00	-
300	Phosphorylation	RFHTVLWTREAVYTM CCEEEEEECHHHEEC	17.92	-
305	Phosphorylation	LWTREAVYTMGLNGG EEECHHHEECCCCCC	9.69	22210691
306	Phosphorylation	WTREAVYTMGLNGGQ EECHHHEECCCCCCC	9.73	22210691
375	Ubiquitination	QCKKMASKQLNLKKV HHHHHHHCCCCCCEE	49.63	-
381	Ubiquitination	SKQLNLKKVLVSGGH HCCCCCCEEEEECCC	43.92	-
475	Ubiquitination	KRKSSEKKEILSNLH HCCCHHHHHHHHHHC	46.03	-
479	Phosphorylation	SEKKEILSNLHNSSS HHHHHHHHHHCCCCC	42.90	23879269
484	Phosphorylation	ILSNLHNSSSDVSYV HHHHHCCCCCCCCHH	22.07	23879269
485	Phosphorylation	LSNLHNSSSDVSYVS HHHHCCCCCCCCHHH	35.56	27251275
486	Phosphorylation	SNLHNSSSDVSYVSD HHHCCCCCCCCHHHC	41.68	27251275
490	Phosphorylation	NSSSDVSYVSDINSV CCCCCCCHHHCHHHH	12.11	27642862
505	Ubiquitination	YERIRLEKLTFAHRA HHHHHHHHCCCCEEE	58.37	-
514	Phosphorylation	TFAHRAVSVSTDPSG CCCEEEEEECCCCCC	14.90	30576142
516	Phosphorylation	AHRAVSVSTDPSGCN CEEEEEECCCCCCCC	21.42	30576142
520	Phosphorylation	VSVSTDPSGCNFAIL EEECCCCCCCCEEEE	59.81	30576142
599	Phosphorylation	LFLSDGNTSEFTDIY HHCCCCCHHHCEEHH	34.85	-
608	Ubiquitination	EFTDIYQKDEDSAGC HCEEHHHCCCCCCCC	46.22	-
630	Phosphorylation	VHPDMFEYLLQFIYT CCHHHHHHHHHHHHH	11.05	17053785
655	Ubiquitination	KPRIHLNKNPEEYQG CCCEECCCCHHHHCH	79.02	-
660	Phosphorylation	LNKNPEEYQGTLNSH CCCCHHHHCHHHHHH	15.58	25627689
663	Phosphorylation	NPEEYQGTLNSHLNK CHHHHCHHHHHHHHC	13.77	28555341
670	Ubiquitination	TLNSHLNKVNFHEDD HHHHHHHCCCCCCCC	44.94	-
680	Ubiquitination	FHEDDNQKSAFEVYK CCCCCCCCCHHHHHH	50.43	-
687	Methylation	KSAFEVYKSNQAQTV CCHHHHHHHHHHCCH	48.37	42353373
687	Ubiquitination	KSAFEVYKSNQAQTV CCHHHHHHHHHHCCH	48.37	2190698
687 (in isoform 1)	Ubiquitination	-	48.37	21906983
687 (in isoform 2)	Ubiquitination	-	48.37	21906983
688	Phosphorylation	SAFEVYKSNQAQTVS CHHHHHHHHHHCCHH	19.25	23403867
695	Phosphorylation	SNQAQTVSERQKSKP HHHHCCHHHHHHCCC	30.37	23403867
725	Ubiquitination	RMLQTVAKKFDFSNL HHHHHHHHHCCCCCH	50.03	-
726	Ubiquitination	MLQTVAKKFDFSNLS HHHHHHHHCCCCCHH	40.19	-
745	Ubiquitination	GVRFENEKINVIAKN CEEECCCEEEEEEEC	51.68	-
751	Ubiquitination	EKINVIAKNTGNKLK CEEEEEEECCCCCEE	43.87	-
765	Phosphorylation	KLSQKKCSFLCDVTM ECCCCCHHHEECCEE	30.08	29449344
771	Phosphorylation	CSFLCDVTMKSVDGK HHHEECCEEEECCCC	12.96	29449344
774	Phosphorylation	LCDVTMKSVDGKEFP EECCEEEECCCCCCC	18.02	29449344
872	Phosphorylation	VALTEKLTLKNAAML HHCCCCCCHHHHHHH	46.91	24719451
886	Phosphorylation	LLEFAAMYSAKQLKL HHHHHHHHCHHHHHH	10.76	26670566
887	Phosphorylation	LEFAAMYSAKQLKLS HHHHHHHCHHHHHHH	18.86	26670566
949	Phosphorylation	YQDGPDISYLEVEDG CCCCCCCEEEEEECC	30.92	-
950	Phosphorylation	QDGPDISYLEVEDGD CCCCCCEEEEEECCC	13.76	-
981	Acetylation	MFKKAKTKAKKKPRK HHHHHHHHCCCCCCC	58.46	69567
984	Acetylation	KAKTKAKKKPRKRSD HHHHHCCCCCCCCCC	73.24	88605
990	Phosphorylation	KKKPRKRSDSSGGYN CCCCCCCCCCCCCCC	44.90	30278072
992	Phosphorylation	KPRKRSDSSGGYNLS CCCCCCCCCCCCCHH	31.93	30278072
993	Phosphorylation	PRKRSDSSGGYNLSD CCCCCCCCCCCCHHH	41.02	30278072
996	Phosphorylation	RSDSSGGYNLSDIIQ CCCCCCCCCHHHHHC	19.22	30278072
999	Phosphorylation	SSGGYNLSDIIQSPS CCCCCCHHHHHCCCC	24.11	23927012
1004	Phosphorylation	NLSDIIQSPSSTGLL CHHHHHCCCCCCCCC	19.15	23927012
1006	Phosphorylation	SDIIQSPSSTGLLKS HHHHCCCCCCCCCCC	45.86	23927012
1007	Phosphorylation	DIIQSPSSTGLLKSG HHHCCCCCCCCCCCC	29.85	23927012
1008	Phosphorylation	IIQSPSSTGLLKSGK HHCCCCCCCCCCCCC	35.87	23927012
1013	Phosphorylation	SSTGLLKSGKTNSVE CCCCCCCCCCCCCCH	45.55	22617229
1016	Phosphorylation	GLLKSGKTNSVESLP CCCCCCCCCCCHHHH	35.93	20873877
1018	Phosphorylation	LKSGKTNSVESLPEL CCCCCCCCCHHHHHH	32.28	29116813
1021	Phosphorylation	GKTNSVESLPELLTS CCCCCCHHHHHHHHC	47.25	30175587
1027	Phosphorylation	ESLPELLTSDSEGSY HHHHHHHHCCCCCCC	43.01	20873877
1028	Phosphorylation	SLPELLTSDSEGSYA HHHHHHHCCCCCCCC	38.82	25159151
1030	Phosphorylation	PELLTSDSEGSYAGV HHHHHCCCCCCCCCC	43.56	27732954
1033	Phosphorylation	LTSDSEGSYAGVGSP HHCCCCCCCCCCCCC	14.04	27732954
1034	Phosphorylation	TSDSEGSYAGVGSPR HCCCCCCCCCCCCCC	20.45	22115753
1039	Phosphorylation	GSYAGVGSPRDLQSP CCCCCCCCCCCCCCC	17.85	26074081
1045	Phosphorylation	GSPRDLQSPDFTTGF CCCCCCCCCCCCCCC	33.46	29255136
1049	Phosphorylation	DLQSPDFTTGFHSDK CCCCCCCCCCCCCCC	33.13	23927012
1050	Phosphorylation	LQSPDFTTGFHSDKI CCCCCCCCCCCCCCC	37.53	23927012
1054	Phosphorylation	DFTTGFHSDKIEAKV CCCCCCCCCCCEEEE	38.03	23927012
1064	Phosphorylation	IEAKVKPYVNGTSPV CEEEEECCCCCCCCC	10.79	28450419
1068	Phosphorylation	VKPYVNGTSPVYSRE EECCCCCCCCCCCHH	25.34	25159151
1069	Phosphorylation	KPYVNGTSPVYSRED ECCCCCCCCCCCHHH	17.48	25159151
1072	Phosphorylation	VNGTSPVYSREDLKP CCCCCCCCCHHHCCC	12.85	28450419
1073	Phosphorylation	NGTSPVYSREDLKPW CCCCCCCCHHHCCCC	29.45	28450419
1078	Ubiquitination	VYSREDLKPWEKSPI CCCHHHCCCCCCCCC	61.48	-
1082	Ubiquitination	EDLKPWEKSPILKIS HHCCCCCCCCCEEEE	58.63	-
1083	Phosphorylation	DLKPWEKSPILKISA HCCCCCCCCCEEEEC	12.98	23401153
1087	Ubiquitination	WEKSPILKISAPQPI CCCCCCEEEECCCCC	35.02	-
1096	Phosphorylation	SAPQPIPSNRIDTTS ECCCCCCCCCCCCCC	39.19	-
1101	Phosphorylation	IPSNRIDTTSSASWV CCCCCCCCCCCCCCC	26.63	28176443
1102	Phosphorylation	PSNRIDTTSSASWVA CCCCCCCCCCCCCCC	19.55	28176443
1103	Phosphorylation	SNRIDTTSSASWVAG CCCCCCCCCCCCCCC	26.46	30576142
1104	Phosphorylation	NRIDTTSSASWVAGS CCCCCCCCCCCCCCC	24.99	30576142
1106	Phosphorylation	IDTTSSASWVAGSFS CCCCCCCCCCCCCCC	24.83	20873877
1111	Phosphorylation	SASWVAGSFSPVSPP CCCCCCCCCCCCCCC	16.89	22496350
1113	Phosphorylation	SWVAGSFSPVSPPVV CCCCCCCCCCCCCEE	26.53	28176443
1116	Phosphorylation	AGSFSPVSPPVVDLR CCCCCCCCCCEECHH	27.20	28176443
1138	Phosphorylation	SRQKCGATPKSHLGK HHHHHCCCCHHHHCC	19.28	-
1140	Methylation	QKCGATPKSHLGKTV HHHCCCCHHHHCCHH	46.26	115971303
1141	Phosphorylation	KCGATPKSHLGKTVS HHCCCCHHHHCCHHH	25.75	-
1145	Methylation	TPKSHLGKTVSHGVK CCHHHHCCHHHHCCC	52.71	115971311
1145	Ubiquitination	TPKSHLGKTVSHGVK CCHHHHCCHHHHCCC	52.71	-
1152	Ubiquitination	KTVSHGVKLSQKQRK CHHHHCCCCCHHHHH	47.19	-
1154	Phosphorylation	VSHGVKLSQKQRKMI HHHCCCCCHHHHHHE	29.61	28555341
1181	Phosphorylation	SMETVLFTPSKAPKP CCCEEEECCCCCCCC	23.61	21815630
1183	Phosphorylation	ETVLFTPSKAPKPVN CEEEECCCCCCCCCC	38.09	27050516
1195	Phosphorylation	PVNAWASSLHSVSSK CCCHHHHHCHHCCCH	23.50	25627689
1198	Phosphorylation	AWASSLHSVSSKSFR HHHHHCHHCCCHHHH	29.05	25159151
1200	Phosphorylation	ASSLHSVSSKSFRDF HHHCHHCCCHHHHHH	34.75	21482705
1202	Ubiquitination	SLHSVSSKSFRDFLL HCHHCCCHHHHHHHH	46.22	-
1203	Phosphorylation	LHSVSSKSFRDFLLE CHHCCCHHHHHHHHH	27.71	21482705
1212	Ubiquitination	RDFLLEEKKSVTSHS HHHHHHHHHCCCCCC	40.76	-
1213	Ubiquitination	DFLLEEKKSVTSHSS HHHHHHHHCCCCCCC	54.13	-
1225	2-Hydroxyisobutyrylation	HSSGDHVKKVSFKGI CCCCCCCEEEEECCC	44.00	-
1230	Methylation	HVKKVSFKGIENSQA CCEEEEECCCCCCCC	52.01	115971319
1230	Acetylation	HVKKVSFKGIENSQA CCEEEEECCCCCCCC	52.01	26051181
1239	Ubiquitination	IENSQAPKIVRCSTH CCCCCCCCEEEEECC	57.77	-
1350	Ubiquitination	LAVPMWNKHGC---- CCCCCCCCCCC----	26.34	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
1200	S	Phosphorylation	Kinase	PRKCB	P05771	GPS
1203	S	Phosphorylation	Kinase	PRKCB	P05771	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of IBTK_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of IBTK_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
BTK_HUMAN	BTK	physical	11577348
BTK_HUMAN	BTK	physical	21482705
LTOR3_HUMAN	LAMTOR3	physical	21988832
CUL3_HUMAN	CUL3	physical	25882842
RBX1_HUMAN	RBX1	physical	25882842
IBTK_HUMAN	IBTK	physical	25882842
CAND1_HUMAN	CAND1	physical	25882842
CSN2_HUMAN	COPS2	physical	25882842
CSN4_HUMAN	COPS4	physical	25882842
UBA1_HUMAN	UBA1	physical	25882842
UBC_HUMAN	UBC	physical	25882842
CSN3_HUMAN	COPS3	physical	25882842
PDCD4_HUMAN	PDCD4	physical	25882842
IF4A1_HUMAN	EIF4A1	physical	26496610
RFC1_HUMAN	RFC1	physical	26496610
RFC3_HUMAN	RFC3	physical	26496610
RFC4_HUMAN	RFC4	physical	26496610
RL6_HUMAN	RPL6	physical	26496610
RLA0_HUMAN	RPLP0	physical	26496610
RLA1_HUMAN	RPLP1	physical	26496610
RS3_HUMAN	RPS3	physical	26496610
RS8_HUMAN	RPS8	physical	26496610
RS9_HUMAN	RPS9	physical	26496610
RS27_HUMAN	RPS27	physical	26496610
SRP72_HUMAN	SRP72	physical	26496610
UBP10_HUMAN	USP10	physical	26496610
DDX21_HUMAN	DDX21	physical	26496610
ESPL1_HUMAN	ESPL1	physical	26496610
POP7_HUMAN	POP7	physical	26496610
DHX30_HUMAN	DHX30	physical	26496610
WDR74_HUMAN	WDR74	physical	26496610
NAT10_HUMAN	NAT10	physical	26496610
TSR1_HUMAN	TSR1	physical	26496610
LACTB_HUMAN	LACTB	physical	26496610
RP25L_HUMAN	RPP25L	physical	26496610
MSL1_HUMAN	MSL1	physical	26496610

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of IBTK_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-990; SER-992; SER-993;SER-1004; SER-1045 AND SER-1054, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1045, AND MASSSPECTROMETRY.	17081983 [Abstract]
"Tyrosine phosphorylated Par3 regulates epithelial tight junctionassembly promoted by EGFR signaling."; Wang Y., Du D., Fang L., Yang G., Zhang C., Zeng R., Ullrich A.,Lottspeich F., Chen Z.; EMBO J. 25:5058-5070(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-630, AND MASSSPECTROMETRY.	17053785 [Abstract]