dbPTM

RAP1B_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RAP1B_HUMAN
UniProt AC	P61224
Protein Name	Ras-related protein Rap-1b
Gene Name	RAP1B
Organism	Homo sapiens (Human).
Sequence Length	184
Subcellular Localization	Cell membrane. Cytoplasm, cytosol. Cell junction. May shuttle between plasma membrane and cytosol. Presence of KRIT1 and CDH5 is required for its localization to the cell junction.
Protein Description	GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function..
Protein Sequence	MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSCQLL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Ubiquitination	---MREYKLVVLGSG ---CCEEEEEEECCC	30.01	21890473
5	Ubiquitination	---MREYKLVVLGSG ---CCEEEEEEECCC	30.01	21890473
5	Acetylation	---MREYKLVVLGSG ---CCEEEEEEECCC	30.01	25953088
5	Ubiquitination	---MREYKLVVLGSG ---CCEEEEEEECCC	30.01	21906983
11	Phosphorylation	YKLVVLGSGGVGKSA EEEEEECCCCCCCHH	28.36	25159151
17	Phosphorylation	GSGGVGKSALTVQFV CCCCCCCHHHHHHEE	23.68	20068231
31	Ubiquitination	VQGIFVEKYDPTIED EEEEEEEECCCCCCH	50.04	21906983
32	Phosphorylation	QGIFVEKYDPTIEDS EEEEEEECCCCCCHH	17.41	20860994
35	Phosphorylation	FVEKYDPTIEDSYRK EEEECCCCCCHHHHH	33.82	23403867
39	Phosphorylation	YDPTIEDSYRKQVEV CCCCCCHHHHHHHEE	17.66	23401153
39	ADP-ribosylation	YDPTIEDSYRKQVEV CCCCCCHHHHHHHEE	17.66	3141412
39	ADP-ribosylation	YDPTIEDSYRKQVEV CCCCCCHHHHHHHEE	17.66	-
40	Phosphorylation	DPTIEDSYRKQVEVD CCCCCHHHHHHHEEC	33.86	23403867
42	Ubiquitination	TIEDSYRKQVEVDAQ CCCHHHHHHHEECHH	50.92	-
58	Phosphorylation	CMLEILDTAGTEQFT HHHHHHHHCCCHHHH	24.44	27732954
61	Phosphorylation	EILDTAGTEQFTAMR HHHHHCCCHHHHHHH	24.93	27732954
65	Phosphorylation	TAGTEQFTAMRDLYM HCCCHHHHHHHHHHH	21.16	27732954
88	Phosphorylation	VYSITAQSTFNDLQD EEEEEECCCCCCHHH	32.28	26657352
117	Ubiquitination	PMILVGNKCDLEDER CEEEECCCCCCCCCE	24.17	-
128	Ubiquitination	EDERVVGKEQGQNLA CCCEECCHHHHHHHH	34.94	-
134	Methylation	GKEQGQNLARQWNNC CHHHHHHHHHHHHCC	2.98	2123345
139	Methylation	QNLARQWNNCAFLES HHHHHHHHCCHHHHH	25.93	2123345
149	Ubiquitination	AFLESSAKSKINVNE HHHHHHCCCCCCHHH	54.98	-
151	Ubiquitination	LESSAKSKINVNEIF HHHHCCCCCCHHHHH	37.28	-
159	Phosphorylation	INVNEIFYDLVRQIN CCHHHHHHHHHHHHC	17.65	23917254
162	Methylation	NEIFYDLVRQINRKT HHHHHHHHHHHCCCC	3.75	2123345
169	Phosphorylation	VRQINRKTPVPGKAR HHHHCCCCCCCCCCC	26.70	28060719
174	Ubiquitination	RKTPVPGKARKKSSC CCCCCCCCCCCCCCC	38.66	-
179	Phosphorylation	PGKARKKSSCQLL-- CCCCCCCCCCCCC--	39.11	12089143
180	Phosphorylation	GKARKKSSCQLL--- CCCCCCCCCCCC---	18.11	8463283
181	Geranylgeranylation	KARKKSSCQLL---- CCCCCCCCCCC----	4.22	2123345
181	Methylation	KARKKSSCQLL---- CCCCCCCCCCC----	4.22	2123345
181	Geranylgeranylation	KARKKSSCQLL---- CCCCCCCCCCC----	4.22	2123345

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
179	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
179	S	Phosphorylation	Kinase	PRKG1	Q13976	GPS
179	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
179	S	Phosphorylation	Kinase	PKG-FAMILY	-	GPS
179	S	Phosphorylation	Kinase	PKA	-	Uniprot
179	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
180	S	Phosphorylation	Kinase	PKACA	P17612	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	SMURF2	Q9HAU4	PMID:17318188

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RAP1B_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RAP1B_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SCG1_HUMAN	CHGB	physical	21900206
A2MG_HUMAN	A2M	physical	21900206
P53_HUMAN	TP53	physical	21900206
CSAD_HUMAN	CSAD	physical	21900206
KPYM_HUMAN	PKM	physical	21900206
ODPB_HUMAN	PDHB	physical	21900206
CAB45_HUMAN	SDF4	physical	21900206
DDAH2_HUMAN	DDAH2	physical	21900206
APLP1_HUMAN	APLP1	physical	21900206
U119A_HUMAN	UNC119	physical	21900206
HEM1_HUMAN	ALAS1	physical	21900206
ZN135_HUMAN	ZNF135	physical	21900206
KMT2B_HUMAN	KMT2B	physical	21900206
TLE1_HUMAN	TLE1	physical	21900206
TCPH_HUMAN	CCT7	physical	21900206
LRIF1_HUMAN	LRIF1	physical	21900206
FAF1_HUMAN	FAF1	physical	21900206
A4_HUMAN	APP	physical	21832049
RGS2_HUMAN	RGS2	physical	21988832
RGL2_HUMAN	RGL2	physical	21988832
RPTOR_HUMAN	RPTOR	physical	24337580
RASF5_HUMAN	RASSF5	physical	25416956
CDC42_HUMAN	CDC42	physical	26344197
RAB5A_HUMAN	RAB5A	physical	26344197
RAB5B_HUMAN	RAB5B	physical	26344197
RAB5C_HUMAN	RAB5C	physical	26344197
RAB7A_HUMAN	RAB7A	physical	26344197
RAB8A_HUMAN	RAB8A	physical	26344197
RAB8B_HUMAN	RAB8B	physical	26344197
STX12_HUMAN	STX12	physical	26344197
STX7_HUMAN	STX7	physical	26344197
QCR2_HUMAN	UQCRC2	physical	26344197
DMXL1_HUMAN	DMXL1	physical	26496610
KLC1_HUMAN	KLC1	physical	26496610
M3K4_HUMAN	MAP3K4	physical	26496610
UBR4_HUMAN	UBR4	physical	26496610
DICER_HUMAN	DICER1	physical	26496610
AP3M1_HUMAN	AP3M1	physical	26496610
GAR1_HUMAN	GAR1	physical	26496610
WAP53_HUMAN	WRAP53	physical	26496610
P33MX_HUMAN	KIAA1191	physical	26496610
SYAM_HUMAN	AARS2	physical	26496610
NCK5L_HUMAN	NCKAP5L	physical	26496610
RT35_HUMAN	MRPS35	physical	26496610
SEN2_HUMAN	TSEN2	physical	26496610
UBP32_HUMAN	USP32	physical	26496610
NRBP2_HUMAN	NRBP2	physical	26496610

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RAP1B_HUMAN

Related Literatures of Post-Translational Modification

Prenylation
Reference	PubMed
"Posttranslationally processed structure of the human platelet proteinsmg p21B: evidence for geranylgeranylation and carboxyl methylation ofthe C-terminal cysteine."; Kawata M., Farnsworth C.C., Yoshida Y., Gelb M.H., Glomset J.A.,Takai Y.; Proc. Natl. Acad. Sci. U.S.A. 87:8960-8964(1990). Cited for: ISOPRENYLATION AT CYS-181.	2123345 [Abstract]