dbPTM

M3K4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	M3K4_HUMAN
UniProt AC	Q9Y6R4
Protein Name	Mitogen-activated protein kinase kinase kinase 4
Gene Name	MAP3K4
Organism	Homo sapiens (Human).
Sequence Length	1608
Subcellular Localization	Cytoplasm, perinuclear region. Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles..
Protein Description	Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6..
Protein Sequence	MREAAAALVPPPAFAVTPAAAMEEPPPPPPPPPPPPEPETESEPECCLAARQEGTLGDSACKSPESDLEDFSDETNTENLYGTSPPSTPRQMKRMSTKHQRNNVGRPASRSNLKEKMNAPNQPPHKDTGKTVENVEEYSYKQEKKIRAALRTTERDRKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIARPARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSGFWLNRSNELIWLELQAWHAGRTINDQDFFLYTARQAIPDIINEILTFKVDYGSFAFVRDRAGFNGTSVEGQCKATPGTKIVGYSTHHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEYEGDDTEGELKELESSTDESEEEQISDPRVPEIRQPIDNSFDIQSRDCISKKLERLESEDDSLGWGAPDWSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKNDRPVEFSEFPDPMWGSDYVQLSRTPPSSEEKCSAVSWEELKAMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLQEVLEDLEKPDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSSASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFRLSAPVRDLLDVLKSKQYVKVQIPGLENLQMFVPDTLAEEKSIILQLLNAAAGKDCSKDSDDVLIDAYLLLTKHGDRARDSEDSWGTWEAQPVKVVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTLCQEQTSSQPVIAKALQQLKNDALELCNRISNAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPMKVPRCHSDPPNPHLIIPTPEGFSTRSMPSDARSHGSPAAAAAAAAAAVAASRPSPSGGDSVLPKSISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRGDSSGSTRRSWELRTLISQSKDTASKLGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
59	Phosphorylation	QEGTLGDSACKSPES CCCCCCCCCCCCCCC	33.49	21815630
63	Phosphorylation	LGDSACKSPESDLED CCCCCCCCCCCCHHC	33.51	30576142
66	Phosphorylation	SACKSPESDLEDFSD CCCCCCCCCHHCCCC	51.37	22115753
72	Phosphorylation	ESDLEDFSDETNTEN CCCHHCCCCCCCCCC	47.03	22115753
75	Phosphorylation	LEDFSDETNTENLYG HHCCCCCCCCCCCCC	53.05	22199227
77	Phosphorylation	DFSDETNTENLYGTS CCCCCCCCCCCCCCC	33.40	22199227
81	Phosphorylation	ETNTENLYGTSPPST CCCCCCCCCCCCCCC	29.91	22199227
83	Phosphorylation	NTENLYGTSPPSTPR CCCCCCCCCCCCCHH	25.22	22199227
84	Phosphorylation	TENLYGTSPPSTPRQ CCCCCCCCCCCCHHH	30.37	25159151
87	Phosphorylation	LYGTSPPSTPRQMKR CCCCCCCCCHHHHHH	55.20	22199227
88	Phosphorylation	YGTSPPSTPRQMKRM CCCCCCCCHHHHHHH	28.41	22199227
98	Acetylation	QMKRMSTKHQRNNVG HHHHHCHHHHHCCCC	30.36	24232467
109	Phosphorylation	NNVGRPASRSNLKEK CCCCCCCCHHHHHHH	38.64	28102081
138	Phosphorylation	TVENVEEYSYKQEKK CCCCHHHHCHHHHHH	12.09	22817900
140	Phosphorylation	ENVEEYSYKQEKKIR CCHHHHCHHHHHHHH	18.78	22817900
152	Phosphorylation	KIRAALRTTERDRKK HHHHHHHCCHHHHHH	33.58	26074081
153	Phosphorylation	IRAALRTTERDRKKN HHHHHHCCHHHHHHC	23.69	26074081
173	Phosphorylation	MLDSVGGSLPKKSIP EEECCCCCCCCCCCC	35.25	20860994
201	O-linked_Glycosylation	SNAKLPVSVPMPIAR CCCCCCCCCCCCCCC	20.68	23301498
213	Phosphorylation	IARPARQTSRTDCPA CCCCCCCCCCCCCCH	18.02	28450419
214	Phosphorylation	ARPARQTSRTDCPAD CCCCCCCCCCCCCHH	25.50	28450419
216	Phosphorylation	PARQTSRTDCPADRL CCCCCCCCCCCHHHH	41.54	28450419
253	Phosphorylation	QRGQENTSGFWLNRS HHCCCCCCCEEECCC	43.50	28555341
300	O-linked_Glycosylation	DIINEILTFKVDYGS HHHHHHHCEECCCCC	27.37	23301498
420	Phosphorylation	VLGIKNLSDIGWPVF HHCCCCHHHCCCCEE	36.25	28176443
431	Phosphorylation	WPVFEIPSPRPSKGN CCEEECCCCCCCCCC	38.92	25159151
435	Phosphorylation	EIPSPRPSKGNEPEY ECCCCCCCCCCCCCC	55.25	28176443
442	Phosphorylation	SKGNEPEYEGDDTEG CCCCCCCCCCCCCHH	35.61	28796482
447	Phosphorylation	PEYEGDDTEGELKEL CCCCCCCCHHHHCHH	51.10	28355574
456	Phosphorylation	GELKELESSTDESEE HHHCHHHCCCCCCHH	51.68	20363803
457	Phosphorylation	ELKELESSTDESEEE HHCHHHCCCCCCHHH	30.42	20363803
458	Phosphorylation	LKELESSTDESEEEQ HCHHHCCCCCCHHHH	53.73	20363803
461	Phosphorylation	LESSTDESEEEQISD HHCCCCCCHHHHHCC	53.79	20363803
467	Phosphorylation	ESEEEQISDPRVPEI CCHHHHHCCCCCCCC	41.05	23909892
481	Phosphorylation	IRQPIDNSFDIQSRD CCCCCCCCCCCCCHH	22.13	21815630
499	Phosphorylation	KKLERLESEDDSLGW HHHHHHHCCCCCCCC	51.41	22617229
503	Phosphorylation	RLESEDDSLGWGAPD HHHCCCCCCCCCCCC	40.89	28102081
512	Phosphorylation	GWGAPDWSTEAGFSR CCCCCCCCCCCCCCH	24.61	26074081
513	Phosphorylation	WGAPDWSTEAGFSRH CCCCCCCCCCCCCHH	26.12	26074081
518	Phosphorylation	WSTEAGFSRHCLTSI CCCCCCCCHHHHHHH	21.87	26074081
582	Phosphorylation	DPMWGSDYVQLSRTP CCCCCCCEEECCCCC	7.56	-
647	Phosphorylation	AGEPSLLSIKQLVRE CCCCCHHHHHHHHHH	32.79	24719451
668	Phosphorylation	GGLLMKQYYQFMLQE HCHHHHHHHHHHHHH	8.17	26657352
669	Phosphorylation	GLLMKQYYQFMLQEV CHHHHHHHHHHHHHH	7.87	26657352
798	Phosphorylation	RRSVIEISRALKELF HHHHHHHHHHHHHHH	9.94	22496350
835	Phosphorylation	IAAEFRLSAPVRDLL HHHHHCCCCCHHHHH	26.69	21406692
890	Acetylation	AAGKDCSKDSDDVLI HCCCCCCCCCCHHHH	68.53	20167786
979	Phosphorylation	LCQEQTSSQPVIAKA HHHHCCCCCHHHHHH	41.72	-
1068	Phosphorylation	KIGDKYISFARKWMN HHHHHHHHHHHHHHH	15.12	-
1076	Phosphorylation	FARKWMNYVLTKCES HHHHHHHHHHHHCCC	4.82	-
1135	Phosphorylation	HVIGKPHSPVTGLYL HHHCCCCCCCCCEEE	29.59	25850435
1138	Phosphorylation	GKPHSPVTGLYLAIH CCCCCCCCCEEEEEC	25.50	23312004
1148	Phosphorylation	YLAIHRNSPRPMKVP EEEECCCCCCCCCCC	23.42	-
1159 (in isoform 2)	Phosphorylation	-	56.27	26657352
1159	Phosphorylation	MKVPRCHSDPPNPHL CCCCCCCCCCCCCCE	56.27	24719451
1178	Phosphorylation	PEGFSTRSMPSDARS CCCCCCCCCCCCHHH	34.82	27251275
1188	Phosphorylation	SDARSHGSPAAAAAA CCHHHCCCHHHHHHH	13.12	22817901
1201	Phosphorylation	AAAAAAVAASRPSPS HHHHHHHHHCCCCCC	8.59	24719451
1217	Phosphorylation	GDSVLPKSISSAHDT CCCCCCCCCCCCCCC	26.26	23403867
1219	Phosphorylation	SVLPKSISSAHDTRG CCCCCCCCCCCCCCC	28.67	23403867
1220	Phosphorylation	VLPKSISSAHDTRGS CCCCCCCCCCCCCCC	28.14	23403867
1224	Phosphorylation	SISSAHDTRGSSVPE CCCCCCCCCCCCCCC	27.36	24719451
1227	Phosphorylation	SAHDTRGSSVPENDR CCCCCCCCCCCCCHH	25.52	26699800
1228	Phosphorylation	AHDTRGSSVPENDRL CCCCCCCCCCCCHHH	44.51	26699800
1232	Phosphorylation	RGSSVPENDRLASIA CCCCCCCCHHHHHHH	33.36	24719451
1246	Phosphorylation	AAELQFRSLSRHSSP HHHHHHHHHHCCCCC	31.80	24850871
1248	Phosphorylation	ELQFRSLSRHSSPTE HHHHHHHHCCCCCCC	29.24	23186163
1251	Phosphorylation	FRSLSRHSSPTEERD HHHHHCCCCCCCCCC	36.58	30266825
1252	Phosphorylation	RSLSRHSSPTEERDE HHHHCCCCCCCCCCC	29.71	30266825
1254	Phosphorylation	LSRHSSPTEERDEPA HHCCCCCCCCCCCCC	53.04	30266825
1262	Phosphorylation	EERDEPAYPRGDSSG CCCCCCCCCCCCCCC	12.27	23403867
1267	Phosphorylation	PAYPRGDSSGSTRRS CCCCCCCCCCCCHHH	39.15	29038488
1268	Phosphorylation	AYPRGDSSGSTRRSW CCCCCCCCCCCHHHH	41.31	16674116
1270	Phosphorylation	PRGDSSGSTRRSWEL CCCCCCCCCHHHHHH	22.23	16674116
1271	Phosphorylation	RGDSSGSTRRSWELR CCCCCCCCHHHHHHH	33.36	29038488
1274	Phosphorylation	SSGSTRRSWELRTLI CCCCCHHHHHHHHHH	22.93	23401153
1279	Phosphorylation	RRSWELRTLISQSKD HHHHHHHHHHHCCCC	40.86	23312004
1282	Phosphorylation	WELRTLISQSKDTAS HHHHHHHHCCCCHHH	31.36	28857561
1284	Phosphorylation	LRTLISQSKDTASKL HHHHHHCCCCHHHHH	26.21	23312004
1287	Phosphorylation	LISQSKDTASKLGPI HHHCCCCHHHHHCHH	36.25	23312004
1324	Phosphorylation	IIGQVCDTPKSYDNV CCCCCCCCCCCCCCE	27.73	28555341
1328	Phosphorylation	VCDTPKSYDNVMHVG CCCCCCCCCCEEEEE	20.05	27642862
1405	Phosphorylation	KHPNLVRYFGVELHR CCCCHHHHCEEEECH	9.37	23312004
1494	Phosphorylation	KLKNNAQTMPGEVNS EEECCCCCCCCCCHH	23.95	27050516
1501	Phosphorylation	TMPGEVNSTLGTAAY CCCCCCHHHHHHHHH	30.13	28348404
1502	Phosphorylation	MPGEVNSTLGTAAYM CCCCCHHHHHHHHHH	24.81	28348404
1505	Phosphorylation	EVNSTLGTAAYMAPE CCHHHHHHHHHHCHH	15.95	19060867
1508	Phosphorylation	STLGTAAYMAPEVIT HHHHHHHHHCHHHHH	7.43	24043423
1515	Phosphorylation	YMAPEVITRAKGEGH HHCHHHHHHHCCCCC	29.74	24043423
1529	Phosphorylation	HGRAADIWSLGCVVI CCHHHHHHHHEEEEE	6.51	24719451
1534	Phosphorylation	DIWSLGCVVIEMVTG HHHHHEEEEEEEHHC	4.63	24719451
1548	Phosphorylation	GKRPWHEYEHNFQIM CCCCCCCCCCCEEEE	15.03	18083107
1556	Phosphorylation	EHNFQIMYKVGMGHK CCCEEEEEEECCCCC	12.26	27259358
1571	Phosphorylation	PPIPERLSPEGKDFL CCCCCCCCCCCCHHH	27.42	24260401
1575	Ubiquitination	ERLSPEGKDFLSHCL CCCCCCCCHHHHHHH	43.58	-
1579	Phosphorylation	PEGKDFLSHCLESDP CCCCHHHHHHHHCCH	16.49	24719451
1584	Phosphorylation	FLSHCLESDPKMRWT HHHHHHHCCHHHCCC	46.38	24719451
1591	Phosphorylation	SDPKMRWTASQLLDH CCHHHCCCHHHHHCC	13.18	22210691

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
1494	T	Phosphorylation	Kinase	MAP3K4	Q9Y6R4	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of M3K4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of M3K4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MP2K1_HUMAN	MAP2K1	physical	9841871
MP2K4_HUMAN	MAP2K4	physical	9841871
MP2K3_HUMAN	MAP2K3	physical	9841871
MP2K6_HUMAN	MAP2K6	physical	9841871
GA45G_HUMAN	GADD45G	physical	9827804
GA45B_HUMAN	GADD45B	physical	9827804
GA45A_HUMAN	GADD45A	physical	9827804
SH3K1_HUMAN	SH3KBP1	physical	16256071
GA45B_HUMAN	GADD45B	physical	16256071
TPR_HUMAN	TPR	physical	20936779
1433Z_HUMAN	YWHAZ	physical	20936779
CNTRL_HUMAN	CNTRL	physical	20936779
SH3K1_HUMAN	SH3KBP1	physical	20221403
TRAF4_HUMAN	TRAF4	physical	16157600
MP2K6_HUMAN	MAP2K6	physical	16157600
MP2K3_HUMAN	MAP2K3	physical	15866172
MP2K6_HUMAN	MAP2K6	physical	15866172
TRAF4_HUMAN	TRAF4	physical	21097508
RIPK2_HUMAN	RIPK2	physical	21097508
MK08_HUMAN	MAPK8	physical	21152872
MP2K6_HUMAN	MAP2K6	physical	17726008
M3K4_HUMAN	MAP3K4	physical	17726008
AXIN1_HUMAN	AXIN1	physical	17726008

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of M3K4_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-499, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-499, AND MASSSPECTROMETRY.	19369195 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-447, AND MASSSPECTROMETRY.	19413330 [Abstract]