NCBP3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NCBP3_HUMAN
• UniProt AC: Q53F19
• Protein Name: Nuclear cap-binding protein subunit 3 {ECO:0000312|HGNC:HGNC:24612}
• Gene Name: NCBP3 {ECO:0000312|HGNC:HGNC:24612}
• Organism: Homo sapiens (Human).
• Sequence Length: 620
• Subcellular Localization: Nucleus . Cytoplasm .
• Protein Description: Associates with NCBP1/CBP80 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export. NCBP3 serves as adapter protein linking the capped RNAs (m7GpppG-capped RNA) to NCBP1/CBP80. Unlike the conventional CBC with NCBP2 which binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus, the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role in only mRNA export. The alternative CBC is particularly important in cellular stress situations such as virus infections and the NCBP3 activity is critical to inhibit virus growth. [PubMed: 26382858]
• Protein Sequence: MAAVRGLRVSVKAEAPAGPALGLPSPEAESGVDRGEPEPMEVEEGELEIVPVRRSLKELIPDTSRRYENKAGSFITGIDVTSKEAIEKKEQRAKRFHFRSEVNLAQRNVALDRDMMKKAIPKVRLETIYICGVDEMSTQDVFSYFKEYPPAHIEWLDDTSCNVVWLDEMTATRALINMSSLPAQDKIRSRDASEDKSAEKRKKDKQEDSSDDDEAEEGEVEDENSSDVELDTLSQVEEESLLRNDLRPANKLAKGNRLFMRFATKDDKKELGAARRSQYYMKYGNPNYGGMKGILSNSWKRRYHSRRIQRDVIKKRALIGDDVGLTSYKHRHSGLVNVPEEPIEEEEEEEEEEEEEEEEDQDMDADDRVVVEYHEELPALKQPRERSASRRSSASSSDSDEMDYDLELKMISTPSPKKSMKMTMYADEVESQLKNIRNSMRADSVSSSNIKNRIGNKLPPEKFADVRHLLDEKRQHSRPRPPVSSTKSDIRQRLGKRPHSPEKAFSSNPVVRREPSSDVHSRLGVPRQDSKGLYADTREKKSGNLWTRLGSAPKTKEKNTKKVDHRAPGAEEDDSELQRAWGALIKEKEQSRQKKSRLDNLPSLQIEVSRESSSGSEAES

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
10	Phosphorylation	AVRGLRVSVKAEAPA CCCCEEEEEEEECCC		27067055
12	Sumoylation	RGLRVSVKAEAPAGP CCEEEEEEEECCCCC		-
12	Sumoylation	RGLRVSVKAEAPAGP CCEEEEEEEECCCCC		28112733
25	Phosphorylation	GPALGLPSPEAESGV CCCCCCCCCCHHCCC		29255136
30	Phosphorylation	LPSPEAESGVDRGEP CCCCCHHCCCCCCCC		30278072
55	Phosphorylation	EIVPVRRSLKELIPD EEEECCHHHHHHCCC		29214152
64	Phosphorylation	KELIPDTSRRYENKA HHHCCCCCHHHCCCC		24719451
67	Phosphorylation	IPDTSRRYENKAGSF CCCCCHHHCCCCCCC		24719451
70	Sumoylation	TSRRYENKAGSFITG CCHHHCCCCCCCCCC		-
70	Sumoylation	TSRRYENKAGSFITG CCHHHCCCCCCCCCC		28112733
70	Ubiquitination	TSRRYENKAGSFITG CCHHHCCCCCCCCCC		29967540
73	Phosphorylation	RYENKAGSFITGIDV HHCCCCCCCCCCCCC		29978859
89	Acetylation	SKEAIEKKEQRAKRF CHHHHHHHHHHHHHH		7708313
94	Acetylation	EKKEQRAKRFHFRSE HHHHHHHHHHHHHHH		7708325
100	Phosphorylation	AKRFHFRSEVNLAQR HHHHHHHHHHHHHHH		27251275
116	Ubiquitination	VALDRDMMKKAIPKV HHCCHHHHHHHCCCC		24816145
133	Ubiquitination	ETIYICGVDEMSTQD EEEEEEECCCCCHHH		24816145
137	Ubiquitination	ICGVDEMSTQDVFSY EEECCCCCHHHHHHH		24816145
154	Ubiquitination	EYPPAHIEWLDDTSC HCCHHHEEECCCCCC		24816145
171	Ubiquitination	VWLDEMTATRALINM EEECHHHHHHHHHCH		24816145
179	Phosphorylation	TRALINMSSLPAQDK HHHHHCHHCCCHHHH		21406692
180	Phosphorylation	RALINMSSLPAQDKI HHHHCHHCCCHHHHH		21406692
182	Ubiquitination	LINMSSLPAQDKIRS HHCHHCCCHHHHHHC		24816145
186	Sumoylation	SSLPAQDKIRSRDAS HCCCHHHHHHCCCCC		28112733
186	Ubiquitination	SSLPAQDKIRSRDAS HCCCHHHHHHCCCCC		29967540
193	Phosphorylation	KIRSRDASEDKSAEK HHHCCCCCCCCHHHH		-
197	Phosphorylation	RDASEDKSAEKRKKD CCCCCCCHHHHHHHH		28985074
207	Ubiquitination	KRKKDKQEDSSDDDE HHHHHHCCCCCCCHH		24816145
209	Phosphorylation	KKDKQEDSSDDDEAE HHHHCCCCCCCHHHH		25137130
210	Phosphorylation	KDKQEDSSDDDEAEE HHHCCCCCCCHHHHH		20363803
225	Phosphorylation	GEVEDENSSDVELDT CCCCCCCCCCCCCHH		20363803
226	Phosphorylation	EVEDENSSDVELDTL CCCCCCCCCCCCHHH		25137130
232	Phosphorylation	SSDVELDTLSQVEEE CCCCCCHHHHHHHHH		18669648
234	Phosphorylation	DVELDTLSQVEEESL CCCCHHHHHHHHHHH		21406692
240	Phosphorylation	LSQVEEESLLRNDLR HHHHHHHHHHCCCCC		21406692
251	Ubiquitination	NDLRPANKLAKGNRL CCCCHHHHHHHCCEE		24816145
268	Ubiquitination	RFATKDDKKELGAAR EECCCCCHHHHHHHH		24816145
281	Ubiquitination	ARRSQYYMKYGNPNY HHHHHHHHHHCCCCC		24816145
292	Methylation	NPNYGGMKGILSNSW CCCCCCCHHHCCCHH		-
296	Phosphorylation	GGMKGILSNSWKRRY CCCHHHCCCHHHHHH		30108239
298	Phosphorylation	MKGILSNSWKRRYHS CHHHCCCHHHHHHHH		29970186
303	Phosphorylation	SNSWKRRYHSRRIQR CCHHHHHHHHHHHHH		22817900
316	Ubiquitination	QRDVIKKRALIGDDV HHHHHHHHHCCCCCC		24816145
326	Phosphorylation	IGDDVGLTSYKHRHS CCCCCCCCCCCCCCC		28555341
327	Phosphorylation	GDDVGLTSYKHRHSG CCCCCCCCCCCCCCC		28555341
327	Ubiquitination	GDDVGLTSYKHRHSG CCCCCCCCCCCCCCC		24816145
329	Acetylation	DVGLTSYKHRHSGLV CCCCCCCCCCCCCCC		25953088
333	Phosphorylation	TSYKHRHSGLVNVPE CCCCCCCCCCCCCCC		17081983
337	Ubiquitination	HRHSGLVNVPEEPIE CCCCCCCCCCCCCCC		24816145
348	Ubiquitination	EPIEEEEEEEEEEEE CCCCHHHHHHHHHHH		24816145
352	Ubiquitination	EEEEEEEEEEEEEEE HHHHHHHHHHHHHHH		24816145
373	Phosphorylation	DDRVVVEYHEELPAL HHCEEEEEHHHCHHH		27642862
373	Ubiquitination	DDRVVVEYHEELPAL HHCEEEEEHHHCHHH		24816145
387	Phosphorylation	LKQPRERSASRRSSA HCCCCHHCHHCCCCC		25137130
389	Phosphorylation	QPRERSASRRSSASS CCCHHCHHCCCCCCC		25137130
392	Phosphorylation	ERSASRRSSASSSDS HHCHHCCCCCCCCCC		25137130
393	Phosphorylation	RSASRRSSASSSDSD HCHHCCCCCCCCCCC		25137130
395	Phosphorylation	ASRRSSASSSDSDEM HHCCCCCCCCCCCCC		25137130
396	Phosphorylation	SRRSSASSSDSDEMD HCCCCCCCCCCCCCC		25137130
397	Phosphorylation	RRSSASSSDSDEMDY CCCCCCCCCCCCCCH		25137130
399	Phosphorylation	SSASSSDSDEMDYDL CCCCCCCCCCCCHHH		33259812
412	Phosphorylation	DLELKMISTPSPKKS HHEEEEECCCCCCHH		23401153
413	Phosphorylation	LELKMISTPSPKKSM HEEEEECCCCCCHHH		29255136
415	Phosphorylation	LKMISTPSPKKSMKM EEEECCCCCCHHHEE		29255136
419	Phosphorylation	STPSPKKSMKMTMYA CCCCCCHHHEEEHHH		26074081
421	Sumoylation	PSPKKSMKMTMYADE CCCCHHHEEEHHHHH		-
421	Sumoylation	PSPKKSMKMTMYADE CCCCHHHEEEHHHHH		-
423	Phosphorylation	PKKSMKMTMYADEVE CCHHHEEEHHHHHHH		26074081
425	Phosphorylation	KSMKMTMYADEVESQ HHHEEEHHHHHHHHH		26074081
426	Ubiquitination	SMKMTMYADEVESQL HHEEEHHHHHHHHHH		24816145
431	Phosphorylation	MYADEVESQLKNIRN HHHHHHHHHHHHHHH		20068231
439	Phosphorylation	QLKNIRNSMRADSVS HHHHHHHHHCCCCCC		30576142
444	Phosphorylation	RNSMRADSVSSSNIK HHHHCCCCCCCHHHH		30576142
446	Phosphorylation	SMRADSVSSSNIKNR HHCCCCCCCHHHHHC		23403867
447	Phosphorylation	MRADSVSSSNIKNRI HCCCCCCCHHHHHCC		21712546
447	Ubiquitination	MRADSVSSSNIKNRI HCCCCCCCHHHHHCC		24816145
448	Phosphorylation	RADSVSSSNIKNRIG CCCCCCCHHHHHCCC		21815630
451	Sumoylation	SVSSSNIKNRIGNKL CCCCHHHHHCCCCCC		-
451	Sumoylation	SVSSSNIKNRIGNKL CCCCHHHHHCCCCCC		-
451	Ubiquitination	SVSSSNIKNRIGNKL CCCCHHHHHCCCCCC		24816145
462	Ubiquitination	GNKLPPEKFADVRHL CCCCCHHHHHCHHHH		24816145
484	Phosphorylation	SRPRPPVSSTKSDIR CCCCCCCCCCHHHHH		22210691
485	Phosphorylation	RPRPPVSSTKSDIRQ CCCCCCCCCHHHHHH		22210691
486	Phosphorylation	PRPPVSSTKSDIRQR CCCCCCCCHHHHHHH		22210691
487	Ubiquitination	RPPVSSTKSDIRQRL CCCCCCCHHHHHHHH		24816145
488	Phosphorylation	PPVSSTKSDIRQRLG CCCCCCHHHHHHHHC		29214152
500	Phosphorylation	RLGKRPHSPEKAFSS HHCCCCCCHHHHHCC		23401153
506	Phosphorylation	HSPEKAFSSNPVVRR CCHHHHHCCCCCEEC		28152594
507	Phosphorylation	SPEKAFSSNPVVRRE CHHHHHCCCCCEECC		28464451
516	Phosphorylation	PVVRREPSSDVHSRL CCEECCCCCCHHHHC		-
517	Phosphorylation	VVRREPSSDVHSRLG CEECCCCCCHHHHCC		-
530	Phosphorylation	LGVPRQDSKGLYADT CCCCCCCCCCCCCCC		28655764
534	Phosphorylation	RQDSKGLYADTREKK CCCCCCCCCCCCCCC		28796482
537	Phosphorylation	SKGLYADTREKKSGN CCCCCCCCCCCCCCC		24702127
541	Sumoylation	YADTREKKSGNLWTR CCCCCCCCCCCHHHC		-
541	Sumoylation	YADTREKKSGNLWTR CCCCCCCCCCCHHHC		28112733
554	Acetylation	TRLGSAPKTKEKNTK HCCCCCCCCCCCCCC		7674127
555	Phosphorylation	RLGSAPKTKEKNTKK CCCCCCCCCCCCCCC		28509920
556	Acetylation	LGSAPKTKEKNTKKV CCCCCCCCCCCCCCC		7674139
561	Ubiquitination	KTKEKNTKKVDHRAP CCCCCCCCCCCCCCC		24816145
575	Phosphorylation	PGAEEDDSELQRAWG CCCCCCHHHHHHHHH		20873877
586	Ubiquitination	RAWGALIKEKEQSRQ HHHHHHHHHHHHHHH		-
596	Phosphorylation	EQSRQKKSRLDNLPS HHHHHHHHHHHCCCC		29523821
603	Phosphorylation	SRLDNLPSLQIEVSR HHHHCCCCCEEEEEC		29523821
609	Phosphorylation	PSLQIEVSRESSSGS CCCEEEEECCCCCCC		22817900
612	Phosphorylation	QIEVSRESSSGSEAE EEEEECCCCCCCCCC		25137130
613	Phosphorylation	IEVSRESSSGSEAES EEEECCCCCCCCCCC		25137130
614	Phosphorylation	EVSRESSSGSEAES- EEECCCCCCCCCCC-		25137130
616	Phosphorylation	SRESSSGSEAES--- ECCCCCCCCCCC---		25137130
620	Phosphorylation	SSGSEAES------- CCCCCCCC-------		25218447

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
100	S	Phosphorylation	Kinase	CHEK1	O14757	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NCBP3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NCBP3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
A4_HUMAN	APP	physical	21832049
NCBP1_HUMAN	NCBP1	physical	22365833
SNIP1_HUMAN	SNIP1	physical	22365833
HNRH3_HUMAN	HNRNPH3	physical	22365833
PLEC_HUMAN	PLEC	physical	26344197
RL6_HUMAN	RPL6	physical	26496610
RL27_HUMAN	RPL27	physical	26496610
SRP19_HUMAN	SRP19	physical	26496610
SPT5H_HUMAN	SUPT5H	physical	26496610
DX39B_HUMAN	DDX39B	physical	26496610
THOC5_HUMAN	THOC5	physical	26496610
ZC11A_HUMAN	ZC3H11A	physical	26496610
THOC1_HUMAN	THOC1	physical	26496610
THOC4_HUMAN	ALYREF	physical	26496610
DX39A_HUMAN	DDX39A	physical	26496610
CKAP2_HUMAN	CKAP2	physical	26496610
HP1B3_HUMAN	HP1BP3	physical	26496610
WDR74_HUMAN	WDR74	physical	26496610
PNO1_HUMAN	PNO1	physical	26496610
THOC2_HUMAN	THOC2	physical	26496610
THOC6_HUMAN	THOC6	physical	26496610
THOC7_HUMAN	THOC7	physical	26496610
UIF_HUMAN	FYTTD1	physical	26496610
CS047_HUMAN	C19orf47	physical	26496610

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.