dbPTM

CDKL5_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	CDKL5_HUMAN
UniProt AC	O76039
Protein Name	Cyclin-dependent kinase-like 5 {ECO:0000305}
Gene Name	CDKL5 {ECO:0000312\|HGNC:HGNC:11411}
Organism	Homo sapiens (Human).
Sequence Length	1030
Subcellular Localization	Nucleus . Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome .
Protein Description	Mediates phosphorylation of MECP2. [PubMed: 15917271]
Protein Sequence	MKIPNIGNVMNKFEILGVVGEGAYGVVLKCRHKETHEIVAIKKFKDSEENEEVKETTLRELKMLRTLKQENIVELKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPPEKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFGFARNLSEGNNANYTEYVATRWYRSPELLLGAPYGKSVDMWSVGCILGELSDGQPLFPGESEIDQLFTIQKVLGPLPSEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILNSVLLDLMKNLLKLDPADRYLTEQCLNHPTFQTQRLLDRSPSRSAKRKPYHVESSTLSNRNQAGKSTALQSHHRSNSKDIQNLSVGLPRADEGLPANESFLNGNLAGASLSPLHTKTYQASSQPGSTSKDLTNNNIPHLLSPKEAKSKTEFDFNIDPKPSEGPGTKYLKSNSRSQQNRHSFMESSQSKAGTLQPNEKQSRHSYIDTIPQSSRSPSYRTKAKSHGALSDSKSVSNLSEARAQIAEPSTSRYFPSSCLDLNSPTSPTPTRHSDTRTLLSPSGRNNRNEGTLDSRRTTTRHSKTMEELKLPEHMDSSHSHSLSAPHESFSYGLGYTSPFSSQQRPHRHSMYVTRDKVRAKGLDGSLSIGQGMAARANSLQLLSPQPGEQLPPEMTVARSSVKETSREGTSSFHTRQKSEGGVYHDPHSDDGTAPKENRHLYNDPVPRRVGSFYRVPSPRPDNSFHENNVSTRVSSLPSESSSGTNHSKRQPAFDPWKSPENISHSEQLKEKEKQGFFRSMKKKKKKSQTVPNSDSPDLLTLQKSIHSASTPSSRPKEWRPEKISDLQTQSQPLKSLRKLLHLSSASNHPASSDPRFQPLTAQQTKNSFSEIRIHPLSQASGGSSNIRQEPAPKGRPALQLPDGGCDGRRQRHHSGPQDRRFMLRTTEQQGEYFCCGDPKKPHTPCVPNRALHRPISSPAPYPVLQVRGTSMCPTLQVRGTDAFSCPTQQSGFSFFVRHVMREALIHRAQVNQAALLTYHENAALTGK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MKIPNIGNV ------CCCCCHHHH	77.50	68381
12	Acetylation	NIGNVMNKFEILGVV CHHHHHHHHEEEEEE	26.69	68385
33	Acetylation	VVLKCRHKETHEIVA EEEEEECCCCEEEEE	44.98	11925771
54	Ubiquitination	SEENEEVKETTLREL HHCCHHHHHHHHHHH	53.26	29967540
68	Ubiquitination	LKMLRTLKQENIVEL HHHHHHHHHCCHHHH	55.76	30230243
128	Ubiquitination	KAIHWCHKNDIVHRD HHHHHHHHCCCCCCC	53.86	29967540
137	Ubiquitination	DIVHRDIKPENLLIS CCCCCCCCHHHEEEC	51.04	30230243
161	Phosphorylation	FGFARNLSEGNNANY HCCCCCCCCCCCCCH	47.18	26356563
168	Phosphorylation	SEGNNANYTEYVATR CCCCCCCHHHHEECC	9.87	28152594
169	Phosphorylation	EGNNANYTEYVATRW CCCCCCHHHHEECCC	22.20	28152594
171	Phosphorylation	NNANYTEYVATRWYR CCCCHHHHEECCCCC	6.38	27273156
174	Phosphorylation	NYTEYVATRWYRSPE CHHHHEECCCCCCHH	16.22	28152594
177	Phosphorylation	EYVATRWYRSPELLL HHEECCCCCCHHHHC	9.64	22817900
188	Phosphorylation	ELLLGAPYGKSVDMW HHHCCCCCCCCHHHH	36.33	22817900
236	Ubiquitination	PLPSEQMKLFYSNPR CCCHHHHHHHHCCCC	34.83	30230243
262	Phosphorylation	PQSLERRYLGILNSV CHHHHHHHHHHHHHH	18.59	22817900
279	Ubiquitination	DLMKNLLKLDPADRY HHHHHHCCCCHHHHH	54.75	30230243
286	Phosphorylation	KLDPADRYLTEQCLN CCCHHHHHHHHHHHC	20.65	21394647
306	Phosphorylation	TQRLLDRSPSRSAKR HHHHHHCCCCCCCCC	27.22	30266825
308	Phosphorylation	RLLDRSPSRSAKRKP HHHHCCCCCCCCCCC	39.86	30266825
314	Ubiquitination	PSRSAKRKPYHVESS CCCCCCCCCCCCCCC	49.58	29967540
316	Phosphorylation	RSAKRKPYHVESSTL CCCCCCCCCCCCCCC	22.87	23663014
320	Phosphorylation	RKPYHVESSTLSNRN CCCCCCCCCCCCCCC	27.77	23663014
321	Phosphorylation	KPYHVESSTLSNRNQ CCCCCCCCCCCCCCC	21.62	23663014
322	Phosphorylation	PYHVESSTLSNRNQA CCCCCCCCCCCCCCC	43.41	23663014
324	Phosphorylation	HVESSTLSNRNQAGK CCCCCCCCCCCCCCH	33.56	23663014
341	Phosphorylation	ALQSHHRSNSKDIQN HHHHHHCCCCHHHHC	40.89	29978859
343	Phosphorylation	QSHHRSNSKDIQNLS HHHHCCCCHHHHCCC	32.71	21712546
350	Phosphorylation	SKDIQNLSVGLPRAD CHHHHCCCCCCCCCC	22.99	27080861
365	Phosphorylation	EGLPANESFLNGNLA CCCCCCHHHCCCCCC	34.54	25850435
375	Phosphorylation	NGNLAGASLSPLHTK CCCCCCCCCCCCCCC	28.58	30266825
377	Phosphorylation	NLAGASLSPLHTKTY CCCCCCCCCCCCCEE	23.95	30266825
381	Phosphorylation	ASLSPLHTKTYQASS CCCCCCCCCEEECCC	32.53	30266825
388	Phosphorylation	TKTYQASSQPGSTSK CCEEECCCCCCCCCC	43.33	28674419
392	Phosphorylation	QASSQPGSTSKDLTN ECCCCCCCCCCCCCC	35.87	25627689
393	Phosphorylation	ASSQPGSTSKDLTNN CCCCCCCCCCCCCCC	45.79	25627689
398	Phosphorylation	GSTSKDLTNNNIPHL CCCCCCCCCCCCCCC	46.14	26074081
407	Phosphorylation	NNIPHLLSPKEAKSK CCCCCCCCHHHHCCC	40.04	30266825
440	Phosphorylation	YLKSNSRSQQNRHSF CCCCCCCCHHHHHHH	35.62	24719451
446	Phosphorylation	RSQQNRHSFMESSQS CCHHHHHHHHHHHHH	24.05	30576142
468	Phosphorylation	NEKQSRHSYIDTIPQ CCHHCCCCCCCCCCC	23.75	28674419
469	Phosphorylation	EKQSRHSYIDTIPQS CHHCCCCCCCCCCCC	9.19	27642862
472	Phosphorylation	SRHSYIDTIPQSSRS CCCCCCCCCCCCCCC	25.62	27251275
476	Phosphorylation	YIDTIPQSSRSPSYR CCCCCCCCCCCCCHH	23.51	25159151
477	Phosphorylation	IDTIPQSSRSPSYRT CCCCCCCCCCCCHHH	31.37	29116813
479	O-linked_Glycosylation	TIPQSSRSPSYRTKA CCCCCCCCCCHHHHH	21.83	30379171
479	Phosphorylation	TIPQSSRSPSYRTKA CCCCCCCCCCHHHHH	21.83	25159151
481	Phosphorylation	PQSSRSPSYRTKAKS CCCCCCCCHHHHHHH	28.61	23312004
482	Phosphorylation	QSSRSPSYRTKAKSH CCCCCCCHHHHHHHC	26.19	23312004
484	Phosphorylation	SRSPSYRTKAKSHGA CCCCCHHHHHHHCCC	27.41	29449344
488	Phosphorylation	SYRTKAKSHGALSDS CHHHHHHHCCCCCCC	31.82	28060719
493	Phosphorylation	AKSHGALSDSKSVSN HHHCCCCCCCCCCCC	39.03	28060719
495	Phosphorylation	SHGALSDSKSVSNLS HCCCCCCCCCCCCHH	24.37	28857561
497	Phosphorylation	GALSDSKSVSNLSEA CCCCCCCCCCCHHHH	34.43	28857561
502	Phosphorylation	SKSVSNLSEARAQIA CCCCCCHHHHHHHHC	33.20	24719451
512	Phosphorylation	RAQIAEPSTSRYFPS HHHHCCCCCCCCCCH	30.44	19664994
516	Phosphorylation	AEPSTSRYFPSSCLD CCCCCCCCCCHHHCC	21.43	29116813
519	Phosphorylation	STSRYFPSSCLDLNS CCCCCCCHHHCCCCC	24.63	27080861
520	Phosphorylation	TSRYFPSSCLDLNSP CCCCCCHHHCCCCCC	20.95	27251275
526	Phosphorylation	SSCLDLNSPTSPTPT HHHCCCCCCCCCCCC	36.53	17192257
528	Phosphorylation	CLDLNSPTSPTPTRH HCCCCCCCCCCCCCC	47.35	30266825
529	Phosphorylation	LDLNSPTSPTPTRHS CCCCCCCCCCCCCCC	30.11	30266825
531	Phosphorylation	LNSPTSPTPTRHSDT CCCCCCCCCCCCCCC	36.74	30266825
533	Phosphorylation	SPTSPTPTRHSDTRT CCCCCCCCCCCCCCE	43.42	30266825
540	Phosphorylation	TRHSDTRTLLSPSGR CCCCCCCEEECCCCC	33.86	22210691
543	O-linked_Glycosylation	SDTRTLLSPSGRNNR CCCCEEECCCCCCCC	21.82	30379171
543	Phosphorylation	SDTRTLLSPSGRNNR CCCCEEECCCCCCCC	21.82	30266825
545	Phosphorylation	TRTLLSPSGRNNRNE CCEEECCCCCCCCCC	47.19	27251275
554	Phosphorylation	RNNRNEGTLDSRRTT CCCCCCCCCCCCCCC	22.63	22210691
557	Phosphorylation	RNEGTLDSRRTTTRH CCCCCCCCCCCCHHC	27.12	22210691
565	Phosphorylation	RRTTTRHSKTMEELK CCCCHHCCCHHHHHC	26.91	29759185
599	Phosphorylation	FSYGLGYTSPFSSQQ CCCCCCCCCCCCCCC	28.29	24275569
603	Phosphorylation	LGYTSPFSSQQRPHR CCCCCCCCCCCCCCC	30.53	24275569
628	Phosphorylation	RAKGLDGSLSIGQGM HHCCCCCCCCHHHHH	20.71	28555341
641	Phosphorylation	GMAARANSLQLLSPQ HHHHHCCCCEEECCC	19.26	27050516
646	Phosphorylation	ANSLQLLSPQPGEQL CCCCEEECCCCCCCC	30.04	17192257
658	Phosphorylation	EQLPPEMTVARSSVK CCCCCCCEEECCCCC	14.89	24275569
681	Phosphorylation	SFHTRQKSEGGVYHD CCEEEEECCCCCCCC	32.89	25159151
686	Phosphorylation	QKSEGGVYHDPHSDD EECCCCCCCCCCCCC	12.09	28450419
704	Phosphorylation	PKENRHLYNDPVPRR CCCCCCCCCCCCCCC	16.05	27642862
714	Phosphorylation	PVPRRVGSFYRVPSP CCCCCCCCEEECCCC	19.08	24719451
720	Phosphorylation	GSFYRVPSPRPDNSF CCEEECCCCCCCCCC	30.59	19369195
726	Phosphorylation	PSPRPDNSFHENNVS CCCCCCCCCCCCCCC	35.05	19369195
760	Ubiquitination	QPAFDPWKSPENISH CCCCCCCCCHHHCCC	62.61	29967540
761	Phosphorylation	PAFDPWKSPENISHS CCCCCCCCHHHCCCH	33.31	30266825
766	Phosphorylation	WKSPENISHSEQLKE CCCHHHCCCHHHHHH	32.32	30266825
768	Phosphorylation	SPENISHSEQLKEKE CHHHCCCHHHHHHHH	21.90	30266825
790	Phosphorylation	MKKKKKKSQTVPNSD HHHHCCCCCCCCCCC	39.71	30622161
792	Phosphorylation	KKKKKSQTVPNSDSP HHCCCCCCCCCCCCC	45.05	30622161
796	Phosphorylation	KSQTVPNSDSPDLLT CCCCCCCCCCCCHHH	33.14	30622161
798	Phosphorylation	QTVPNSDSPDLLTLQ CCCCCCCCCCHHHHH	21.82	30622161
803	Phosphorylation	SDSPDLLTLQKSIHS CCCCCHHHHHHHHHH	34.15	30622161
812	Phosphorylation	QKSIHSASTPSSRPK HHHHHHCCCCCCCCC	43.81	25627689
813	Phosphorylation	KSIHSASTPSSRPKE HHHHHCCCCCCCCCC	27.23	28348404
831	Phosphorylation	EKISDLQTQSQPLKS HHHCHHHHCCCHHHH	37.17	-
833	Phosphorylation	ISDLQTQSQPLKSLR HCHHHHCCCHHHHHH	36.83	-
847	Phosphorylation	RKLLHLSSASNHPAS HHHHHHHHHCCCCCC	41.92	28555341
870	Phosphorylation	TAQQTKNSFSEIRIH CHHHHCCCCCEEEEE	31.53	27251275
983	Phosphorylation	PTLQVRGTDAFSCPT CCEEEECCCCCCCCC	17.37	-
987	Phosphorylation	VRGTDAFSCPTQQSG EECCCCCCCCCCCCC	22.40	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
169	T	Phosphorylation	Kinase	CDKL5	O76039	PSP
171	Y	Phosphorylation	Kinase	CDKL5	O76039	PSP
308	S	Phosphorylation	Kinase	DYRK1A	Q13627	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of CDKL5_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of CDKL5_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MECP2_HUMAN	MECP2	physical	15917271
CDKL5_HUMAN	CDKL5	physical	16935860
SKIV2_HUMAN	SKIV2L	physical	21988832
CDKL5_HUMAN	CDKL5	physical	23602568
KGP2_HUMAN	PRKG2	physical	23602568
SPB8_HUMAN	SERPINB8	physical	26186194
HDGR2_HUMAN	HDGFRP2	physical	26186194
DHX16_HUMAN	DHX16	physical	26186194
MACF1_HUMAN	MACF1	physical	26186194
GPAM1_HUMAN	GPALPP1	physical	26186194
KLH20_HUMAN	KLHL20	physical	26186194
SAR1B_HUMAN	SAR1B	physical	26186194
RABL6_HUMAN	RABL6	physical	26186194
UBAP2_HUMAN	UBAP2	physical	26186194
PRP16_HUMAN	DHX38	physical	26186194
PRP16_HUMAN	DHX38	physical	28514442
MACF1_HUMAN	MACF1	physical	28514442
SPB8_HUMAN	SERPINB8	physical	28514442
DHX16_HUMAN	DHX16	physical	28514442
GPAM1_HUMAN	GPALPP1	physical	28514442
KLH20_HUMAN	KLHL20	physical	28514442
HDGR3_HUMAN	HDGFRP3	physical	28514442
RABL6_HUMAN	RABL6	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
	Note=Chromosomal aberrations involving CDKL5 are found in patients manifesting early-onset seizures and spams and psychomotor impairment. Translocation t(X
	6)(p22.3
	q14)
	translocation t(X
	7)(p22.3
	p15).
300672
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of CDKL5_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-171; SER-306; SER-375;SER-377; SER-407; SER-476; SER-488; SER-529; SER-681; SER-720 ANDSER-726, AND MASS SPECTROMETRY.	19369195 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407, AND MASSSPECTROMETRY.	18669648 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-171; SER-306; SER-407;SER-646; SER-720; SER-726 AND SER-761, AND MASS SPECTROMETRY.	18691976 [Abstract]