MISP_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MISP_HUMAN
• UniProt AC: Q8IVT2
• Protein Name: Mitotic interactor and substrate of PLK1 {ECO:0000303|PubMed:23574715}
• Gene Name: MISP {ECO:0000312|HGNC:HGNC:27000}
• Organism: Homo sapiens (Human).
• Sequence Length: 679
• Subcellular Localization: Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Predominantly localizes to cortical actin structures during interphase and mitosis. Present in retraction fibers, which are formed at former adhesion sites during mitosis
• Protein Description: Plays a role in mitotic spindle orientation and mitotic progression. Regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning. May link microtubules to the actin cytospkeleton and focal adhesions. May be required for directed cell migration and centrosome orientation. May also be necessary for proper stacking of the Golgi apparatus..
• Protein Sequence: MDRVTRYPILGIPQAHRGTGLVLDGDTSYTYHLVCMGPEASGWGQDEPQTWPTDHRAQQGVQRQGVSYSVHAYTGQPSPRGLHSENREDEGWQVYRLGARDAHQGRPTWALRPEDGEDKEMKTYRLDAGDADPRRLCDLERERWAVIQGQAVRKSSTVATLQGTPDHGDPRTPGPPRSTPLEENVVDREQIDFLAARQQFLSLEQANKGAPHSSPARGTPAGTTPGASQAPKAFNKPHLANGHVVPIKPQVKGVVREENKVRAVPTWASVQVVDDPGSLASVESPGTPKETPIEREIRLAQEREADLREQRGLRQATDHQELVEIPTRPLLTKLSLITAPRRERGRPSLYVQRDIVQETQREEDHRREGLHVGRASTPDWVSEGPQPGLRRALSSDSILSPAPDARAADPAPEVRKVNRIPPDAYQPYLSPGTPQLEFSAFGAFGKPSSLSTAEAKAATSPKATMSPRHLSESSGKPLSTKQEASKPPRGCPQANRGVVRWEYFRLRPLRFRAPDEPQQAQVPHVWGWEVAGAPALRLQKSQSSDLLERERESVLRREQEVAEERRNALFPEVFSPTPDENSDQNSRSSSQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPVDSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Phosphorylation	---MDRVTRYPILGI ---CCCCCCCCCCCC		21945579
6	Dimethylation	--MDRVTRYPILGIP --CCCCCCCCCCCCC		-
6	Methylation	--MDRVTRYPILGIP --CCCCCCCCCCCCC		54554861
7	Phosphorylation	-MDRVTRYPILGIPQ -CCCCCCCCCCCCCC		21945579
27	Phosphorylation	GLVLDGDTSYTYHLV CEEECCCCCEEEEEE		27642862
28	Phosphorylation	LVLDGDTSYTYHLVC EEECCCCCEEEEEEE		27642862
29	Phosphorylation	VLDGDTSYTYHLVCM EECCCCCEEEEEEEE		27642862
30	Phosphorylation	LDGDTSYTYHLVCMG ECCCCCEEEEEEEEC		27642862
31	Phosphorylation	DGDTSYTYHLVCMGP CCCCCEEEEEEEECC		22817900
67	Phosphorylation	GVQRQGVSYSVHAYT CHHHCCCEEEEEEEC		21945579
68	Phosphorylation	VQRQGVSYSVHAYTG HHHCCCEEEEEEECC		21945579
69	Phosphorylation	QRQGVSYSVHAYTGQ HHCCCEEEEEEECCC		21945579
73	Phosphorylation	VSYSVHAYTGQPSPR CEEEEEEECCCCCCC		26462736
74	Phosphorylation	SYSVHAYTGQPSPRG EEEEEEECCCCCCCC		21945579
78	Phosphorylation	HAYTGQPSPRGLHSE EEECCCCCCCCCCCC		21945579
84	Phosphorylation	PSPRGLHSENREDEG CCCCCCCCCCCCCCC		26657352
95	Phosphorylation	EDEGWQVYRLGARDA CCCCCEEEECCCCCC		24732914
124	Phosphorylation	EDKEMKTYRLDAGDA CCCCCEEEECCCCCC		-
154	Ubiquitination	IQGQAVRKSSTVATL EECCEEECCCCEEEE		29967540
155	Phosphorylation	QGQAVRKSSTVATLQ ECCEEECCCCEEEEC		30266825
156	Phosphorylation	GQAVRKSSTVATLQG CCEEECCCCEEEECC		30266825
157	Phosphorylation	QAVRKSSTVATLQGT CEEECCCCEEEECCC		30266825
160	Phosphorylation	RKSSTVATLQGTPDH ECCCCEEEECCCCCC		30266825
164	Phosphorylation	TVATLQGTPDHGDPR CEEEECCCCCCCCCC		30266825
172	Phosphorylation	PDHGDPRTPGPPRST CCCCCCCCCCCCCCC		27273156
178	O-linked_Glycosylation	RTPGPPRSTPLEENV CCCCCCCCCCCHHCC		30379171
178	Phosphorylation	RTPGPPRSTPLEENV CCCCCCCCCCCHHCC		30266825
179	Phosphorylation	TPGPPRSTPLEENVV CCCCCCCCCCHHCCC		30266825
202	Phosphorylation	AARQQFLSLEQANKG HHHHHHHCHHHHHCC		24732914
208	Acetylation	LSLEQANKGAPHSSP HCHHHHHCCCCCCCC		26051181
208	Ubiquitination	LSLEQANKGAPHSSP HCHHHHHCCCCCCCC		29967540
213	Phosphorylation	ANKGAPHSSPARGTP HHCCCCCCCCCCCCC		25159151
214	Phosphorylation	NKGAPHSSPARGTPA HCCCCCCCCCCCCCC		25159151
219	Phosphorylation	HSSPARGTPAGTTPG CCCCCCCCCCCCCCC		25159151
223	Phosphorylation	ARGTPAGTTPGASQA CCCCCCCCCCCHHHC		25159151
224	Phosphorylation	RGTPAGTTPGASQAP CCCCCCCCCCHHHCC		25159151
228	Phosphorylation	AGTTPGASQAPKAFN CCCCCCHHHCCHHHC		24732914
236	Acetylation	QAPKAFNKPHLANGH HCCHHHCCCCCCCCE		26051181
248	Acetylation	NGHVVPIKPQVKGVV CCEEEECCCCCEEEE		26051181
266	Phosphorylation	NKVRAVPTWASVQVV CEEEEECEEEEEEEE		24732914
269	Phosphorylation	RAVPTWASVQVVDDP EEECEEEEEEEECCC		24732914
278	Phosphorylation	QVVDDPGSLASVESP EEECCCCCCCCEECC		24732914
281	Phosphorylation	DDPGSLASVESPGTP CCCCCCCCEECCCCC		30175587
284	Phosphorylation	GSLASVESPGTPKET CCCCCEECCCCCCCC		30266825
287	Phosphorylation	ASVESPGTPKETPIE CCEECCCCCCCCCCH		30266825
291	Phosphorylation	SPGTPKETPIEREIR CCCCCCCCCCHHHHH		22210691
327	Phosphorylation	QELVEIPTRPLLTKL HHHHHCCCHHHHHHH		24719451
332	Phosphorylation	IPTRPLLTKLSLITA CCCHHHHHHHHEEEC		24732914
333	Acetylation	PTRPLLTKLSLITAP CCHHHHHHHHEEECC		26051181
335	Phosphorylation	RPLLTKLSLITAPRR HHHHHHHHEEECCCH		30266825
338	Phosphorylation	LTKLSLITAPRRERG HHHHHEEECCCHHCC		30266825
348	Phosphorylation	RRERGRPSLYVQRDI CHHCCCCCEEEEHHH		30266825
350	Phosphorylation	ERGRPSLYVQRDIVQ HCCCCCEEEEHHHHH		24732914
376	Phosphorylation	GLHVGRASTPDWVSE CCCCCCCCCCHHHHC		30266825
377	Phosphorylation	LHVGRASTPDWVSEG CCCCCCCCCHHHHCC		22167270
382	Phosphorylation	ASTPDWVSEGPQPGL CCCCHHHHCCCCCCH		22167270
394	Phosphorylation	PGLRRALSSDSILSP CCHHHHCCCCCCCCC		25159151
395	Phosphorylation	GLRRALSSDSILSPA CHHHHCCCCCCCCCC		22167270
397	Phosphorylation	RRALSSDSILSPAPD HHHCCCCCCCCCCCC		22167270
400	Phosphorylation	LSSDSILSPAPDARA CCCCCCCCCCCCCCC		23927012
425	Phosphorylation	NRIPPDAYQPYLSPG CCCCCCCCCCCCCCC		24732914
428	Phosphorylation	PPDAYQPYLSPGTPQ CCCCCCCCCCCCCCC		24732914
430	Phosphorylation	DAYQPYLSPGTPQLE CCCCCCCCCCCCCEE		25159151
433	Phosphorylation	QPYLSPGTPQLEFSA CCCCCCCCCCEEEEE		25159151
439	Phosphorylation	GTPQLEFSAFGAFGK CCCCEEEEEECCCCC		24732914
448	Phosphorylation	FGAFGKPSSLSTAEA ECCCCCCCCCCHHHH		24732914
449	Phosphorylation	GAFGKPSSLSTAEAK CCCCCCCCCCHHHHH		26657352
451	Phosphorylation	FGKPSSLSTAEAKAA CCCCCCCCHHHHHHC		24732914
452	Phosphorylation	GKPSSLSTAEAKAAT CCCCCCCHHHHHHCC		24732914
456	Ubiquitination	SLSTAEAKAATSPKA CCCHHHHHHCCCCCC		29967540
459	Phosphorylation	TAEAKAATSPKATMS HHHHHHCCCCCCCCC		24732914
460	Phosphorylation	AEAKAATSPKATMSP HHHHHCCCCCCCCCH		24732914
464	Phosphorylation	AATSPKATMSPRHLS HCCCCCCCCCHHHHH		24732914
466	O-linked_Glycosylation	TSPKATMSPRHLSES CCCCCCCCHHHHHCC		30379171
466	Phosphorylation	TSPKATMSPRHLSES CCCCCCCCHHHHHCC		25159151
471	Phosphorylation	TMSPRHLSESSGKPL CCCHHHHHCCCCCCC		23927012
473	Phosphorylation	SPRHLSESSGKPLST CHHHHHCCCCCCCCC		30266825
474	Phosphorylation	PRHLSESSGKPLSTK HHHHHCCCCCCCCCC		23927012
476	Acetylation	HLSESSGKPLSTKQE HHHCCCCCCCCCCCH		26051181
479	Phosphorylation	ESSGKPLSTKQEASK CCCCCCCCCCCHHCC		25159151
480	Phosphorylation	SSGKPLSTKQEASKP CCCCCCCCCCHHCCC		29632367
485	Phosphorylation	LSTKQEASKPPRGCP CCCCCHHCCCCCCCC		24732914
540	Ubiquitination	APALRLQKSQSSDLL CCHHCCCCCCCCHHH		-
541	Phosphorylation	PALRLQKSQSSDLLE CHHCCCCCCCCHHHH		25159151
543	Phosphorylation	LRLQKSQSSDLLERE HCCCCCCCCHHHHHH		30266825
544	Phosphorylation	RLQKSQSSDLLERER CCCCCCCCHHHHHHH		30266825
553	Phosphorylation	LLERERESVLRREQE HHHHHHHHHHHHHHH		23401153
575	Phosphorylation	ALFPEVFSPTPDENS CCCCCCCCCCCCCCC		22167270
577	Phosphorylation	FPEVFSPTPDENSDQ CCCCCCCCCCCCCCC		30266825
582	Phosphorylation	SPTPDENSDQNSRSS CCCCCCCCCCCCCCC		30266825
586	Phosphorylation	DENSDQNSRSSSQAS CCCCCCCCCCCHHCC		26055452
590	Phosphorylation	DQNSRSSSQASGITG CCCCCCCHHCCCCCC		29496963
598	Phosphorylation	QASGITGSYSVSESP HCCCCCCCEECCCCC		28348404
600	Phosphorylation	SGITGSYSVSESPFF CCCCCCEECCCCCCC		28348404
602	Phosphorylation	ITGSYSVSESPFFSP CCCCEECCCCCCCCC		28348404
604	Phosphorylation	GSYSVSESPFFSPIH CCEECCCCCCCCCEE		28348404
608	Phosphorylation	VSESPFFSPIHLHSN CCCCCCCCCEEEECC		19007248
614	Phosphorylation	FSPIHLHSNVAWTVE CCCEEEECCEEEEEC		28348404
619	Phosphorylation	LHSNVAWTVEDPVDS EECCEEEEECCCCCC		28348404
634	Ubiquitination	APPGQRKKEQWYAGI CCCCCCCCCEECCCC		29967540
638	Phosphorylation	QRKKEQWYAGINPSD CCCCCEECCCCCCCC		25394399
673	Phosphorylation	ERWESRIYASEEDD- HHHHHHCCCCCCCC-		27251275
675	Phosphorylation	WESRIYASEEDD--- HHHHCCCCCCCC---		25159151

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
78	S	Phosphorylation	Kinase	CDK1	P06493	Uniprot
164	T	Phosphorylation	Kinase	CDK1	P06493	Uniprot
172	T	Phosphorylation	Kinase	CDK1	P06493	Uniprot
214	S	Phosphorylation	Kinase	CDK1	P06493	Uniprot
224	T	Phosphorylation	Kinase	CDK1	P06493	Uniprot
284	S	Phosphorylation	Kinase	CDK1	P06493	Uniprot
287	T	Phosphorylation	Kinase	CDK1	P06493	Uniprot
348	S	Phosphorylation	Kinase	AURKB	Q96GD4	GPS
377	T	Phosphorylation	Kinase	CDK1	P06493	Uniprot
382	S	Phosphorylation	Kinase	CDK1	P06493	Uniprot
394	S	Phosphorylation	Kinase	PLK1	P53350	Uniprot
395	S	Phosphorylation	Kinase	PLK1	P53350	Uniprot
397	S	Phosphorylation	Kinase	PLK1	P53350	Uniprot
471	S	Phosphorylation	Kinase	PLK1	P53350	Uniprot
575	S	Phosphorylation	Kinase	CDK1	P06493	Uniprot
582	S	Phosphorylation	Kinase	PLK1	P53350	Uniprot
586	S	Phosphorylation	Kinase	PLK1	P53350	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MISP_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MISP_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
E41L1_HUMAN	EPB41L1	physical	28514442
KS6A3_HUMAN	RPS6KA3	physical	28514442
KS6A1_HUMAN	RPS6KA1	physical	28514442
KS6A2_HUMAN	RPS6KA2	physical	28514442
OBSL1_HUMAN	OBSL1	physical	28514442
DYR1B_HUMAN	DYRK1B	physical	28514442
KLDC3_HUMAN	KLHDC3	physical	28514442
E41L3_HUMAN	EPB41L3	physical	28514442
FARP2_HUMAN	FARP2	physical	28514442
RABEK_HUMAN	RABEPK	physical	28514442
DCAM_HUMAN	AMD1	physical	28514442
EZRI_HUMAN	EZR	physical	28514442
MOES_HUMAN	MSN	physical	28514442
RADI_HUMAN	RDX	physical	28514442
HS12A_HUMAN	HSPA12A	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.