UniProt ID | RSP5_YEAST | |
---|---|---|
UniProt AC | P39940 | |
Protein Name | E3 ubiquitin-protein ligase RSP5 | |
Gene Name | RSP5 | |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
Sequence Length | 809 | |
Subcellular Localization | Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, actin patch . | |
Protein Description | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other cell surface proteins like GAP1, FUR4, MAL61, PMA1 and STE2. The RSP5-BUL1/2 complex is also necessary for the heat-shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription, pressure-induced differential regulation of the two tryptophan permeases TAT1 and TAT2 and sorting efficiency into multivesicular bodies. Also acts on RBP1. Plays a role in tolerance to o-dinitrobenzene. Involved in actin cytoskeleton organization and dynamics. Ubiquitinates the LAS17-binding proteins LSB1 and PIN3/LSB2 without directing them for degradation and affects LAS17 levels in a SLA1-dependent and LSB1/2-independent manner.. | |
Protein Sequence | MPSSISVKLVAAESLYKRDVFRSPDPFAVLTIDGYQTKSTSAAKKTLNPYWNETFKFDDINENSILTIQVFDQKKFKKKDQGFLGVVNVRVGDVLGHLDEDTATSSGRPREETITRDLKKSNDGMAVSGRLIVVLSKLPSSSPHSQAPSGHTASSSTNTSSTTRTNGHSTSSTRNHSTSHPSRGTAQAVESTLQSGTTAATNTATTSHRSTNSTSSATRQYSSFEDQYGRLPPGWERRTDNFGRTYYVDHNTRTTTWKRPTLDQTEAERGNQLNANTELERRQHRGRTLPGGSSDNSSVTVQVGGGSNIPPVNGAAAAAFAATGGTTSGLGELPSGWEQRFTPEGRAYFVDHNTRTTTWVDPRRQQYIRTYGPTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNVPQYKRDFRRKVIYFRSQPALRILPGQCHIKVRRKNIFEDAYQEIMRQTPEDLKKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEYSAYDNYTIQINPNSGINPEHLNYFKFIGRVVGLGVFHRRFLDAFFVGALYKMMLRKKVVLQDMEGVDAEVYNSLNWMLENSIDGVLDLTFSADDERFGEVVTVDLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQFKAFMDGFNELIPEDLVTVFDERELELLIGGIAEIDIEDWKKHTDYRGYQESDEVIQWFWKCVSEWDNEQRARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAGEVQQLPKSHTCFNRVDLPQYVDYDSMKQKLTLAVEETIGFGQE | |
Overview of Protein Modification Sites with Functional and Structural Information | ||
* ASA = Accessible Surface Area
Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
---|---|---|---|---|---|
14 | Phosphorylation | VKLVAAESLYKRDVF EEEEEEHHHHHCCCC | 32.07 | 22369663 | |
16 | Phosphorylation | LVAAESLYKRDVFRS EEEEHHHHHCCCCCC | 17.16 | 22369663 | |
17 | Acetylation | VAAESLYKRDVFRSP EEEHHHHHCCCCCCC | 47.63 | 24489116 | |
17 | Succinylation | VAAESLYKRDVFRSP EEEHHHHHCCCCCCC | 47.63 | 23954790 | |
45 | Acetylation | KSTSAAKKTLNPYWN CCCCHHHHHCCCHHC | 54.22 | 22865919 | |
45 | Ubiquitination | KSTSAAKKTLNPYWN CCCCHHHHHCCCHHC | 54.22 | 23749301 | |
78 | Acetylation | FDQKKFKKKDQGFLG ECHHHCCCCCCCEEE | 65.79 | 24489116 | |
78 | Ubiquitination | FDQKKFKKKDQGFLG ECHHHCCCCCCCEEE | 65.79 | 22106047 | |
79 | Ubiquitination | DQKKFKKKDQGFLGV CHHHCCCCCCCEEEE | 56.74 | 23749301 | |
79 | Acetylation | DQKKFKKKDQGFLGV CHHHCCCCCCCEEEE | 56.74 | 24489116 | |
105 | Phosphorylation | LDEDTATSSGRPREE CCCCCCCCCCCCCHH | 28.47 | 27214570 | |
121 | Phosphorylation | ITRDLKKSNDGMAVS HHHHHHHCCCCCEEC | 38.27 | 22369663 | |
128 | Phosphorylation | SNDGMAVSGRLIVVL CCCCCEECCEEEEEE | 14.52 | 22369663 | |
136 | Phosphorylation | GRLIVVLSKLPSSSP CEEEEEEECCCCCCC | 22.12 | 19823750 | |
140 | Phosphorylation | VVLSKLPSSSPHSQA EEEECCCCCCCCCCC | 54.61 | 22890988 | |
141 | Phosphorylation | VLSKLPSSSPHSQAP EEECCCCCCCCCCCC | 46.05 | 22890988 | |
142 | Phosphorylation | LSKLPSSSPHSQAPS EECCCCCCCCCCCCC | 30.85 | 22890988 | |
145 | Phosphorylation | LPSSSPHSQAPSGHT CCCCCCCCCCCCCCC | 31.27 | 22890988 | |
149 | Phosphorylation | SPHSQAPSGHTASSS CCCCCCCCCCCCCCC | 46.34 | 22890988 | |
152 | Phosphorylation | SQAPSGHTASSSTNT CCCCCCCCCCCCCCC | 31.59 | 22890988 | |
154 | Phosphorylation | APSGHTASSSTNTSS CCCCCCCCCCCCCCC | 26.44 | 22890988 | |
155 | Phosphorylation | PSGHTASSSTNTSST CCCCCCCCCCCCCCC | 38.42 | 22890988 | |
156 | Phosphorylation | SGHTASSSTNTSSTT CCCCCCCCCCCCCCC | 24.15 | 22890988 | |
157 | Phosphorylation | GHTASSSTNTSSTTR CCCCCCCCCCCCCCC | 44.45 | 22890988 | |
159 | Phosphorylation | TASSSTNTSSTTRTN CCCCCCCCCCCCCCC | 24.92 | 22890988 | |
160 | Phosphorylation | ASSSTNTSSTTRTNG CCCCCCCCCCCCCCC | 27.97 | 22890988 | |
161 | Phosphorylation | SSSTNTSSTTRTNGH CCCCCCCCCCCCCCC | 31.59 | 22890988 | |
162 | Phosphorylation | SSTNTSSTTRTNGHS CCCCCCCCCCCCCCC | 21.77 | 22890988 | |
163 | Phosphorylation | STNTSSTTRTNGHST CCCCCCCCCCCCCCC | 37.13 | 28889911 | |
177 | Phosphorylation | TSSTRNHSTSHPSRG CCCCCCCCCCCCCHH | 34.84 | 28889911 | |
179 | Phosphorylation | STRNHSTSHPSRGTA CCCCCCCCCCCHHHH | 36.09 | 28889911 | |
182 | Phosphorylation | NHSTSHPSRGTAQAV CCCCCCCCHHHHHHH | 37.61 | 30377154 | |
185 | Phosphorylation | TSHPSRGTAQAVEST CCCCCHHHHHHHHHH | 17.93 | 19779198 | |
191 | Phosphorylation | GTAQAVESTLQSGTT HHHHHHHHHHHCCCE | 28.02 | 23749301 | |
192 | Phosphorylation | TAQAVESTLQSGTTA HHHHHHHHHHCCCEE | 18.47 | 19779198 | |
195 | Phosphorylation | AVESTLQSGTTAATN HHHHHHHCCCEEECC | 41.02 | 23749301 | |
197 | Phosphorylation | ESTLQSGTTAATNTA HHHHHCCCEEECCCC | 20.64 | 28889911 | |
198 | Phosphorylation | STLQSGTTAATNTAT HHHHCCCEEECCCCC | 20.16 | 28889911 | |
201 | Phosphorylation | QSGTTAATNTATTSH HCCCEEECCCCCCCC | 30.55 | 23749301 | |
203 | Phosphorylation | GTTAATNTATTSHRS CCEEECCCCCCCCCC | 22.49 | 28889911 | |
206 | Phosphorylation | AATNTATTSHRSTNS EECCCCCCCCCCCCC | 21.77 | 30377154 | |
207 | Phosphorylation | ATNTATTSHRSTNST ECCCCCCCCCCCCCC | 16.73 | 25752575 | |
210 | Phosphorylation | TATTSHRSTNSTSSA CCCCCCCCCCCCCCC | 26.90 | 22369663 | |
211 | Phosphorylation | ATTSHRSTNSTSSAT CCCCCCCCCCCCCCC | 32.90 | 22369663 | |
213 | Phosphorylation | TSHRSTNSTSSATRQ CCCCCCCCCCCCCCC | 29.74 | 22369663 | |
214 | Phosphorylation | SHRSTNSTSSATRQY CCCCCCCCCCCCCCC | 28.86 | 22369663 | |
215 | Phosphorylation | HRSTNSTSSATRQYS CCCCCCCCCCCCCCC | 20.14 | 22369663 | |
216 | Phosphorylation | RSTNSTSSATRQYSS CCCCCCCCCCCCCCC | 33.47 | 22369663 | |
218 | Phosphorylation | TNSTSSATRQYSSFE CCCCCCCCCCCCCCH | 21.91 | 22369663 | |
221 | Phosphorylation | TSSATRQYSSFEDQY CCCCCCCCCCCHHHC | 11.73 | 22369663 | |
222 | Phosphorylation | SSATRQYSSFEDQYG CCCCCCCCCCHHHCC | 21.88 | 22369663 | |
223 | Phosphorylation | SATRQYSSFEDQYGR CCCCCCCCCHHHCCC | 27.93 | 22369663 | |
228 | Phosphorylation | YSSFEDQYGRLPPGW CCCCHHHCCCCCCCC | 19.28 | 22369663 | |
258 | Ubiquitination | NTRTTTWKRPTLDQT CCCEEECCCCCCCHH | 46.54 | 23749301 | |
258 | Acetylation | NTRTTTWKRPTLDQT CCCEEECCCCCCCHH | 46.54 | 24489116 | |
261 | Phosphorylation | TTTWKRPTLDQTEAE EEECCCCCCCHHHHH | 47.18 | 28889911 | |
411 | Ubiquitination | YFVDHNTKTTTWDDP EEEECCCCCCCCCCC | 49.55 | 23749301 | |
432 | Ubiquitination | DQNVPQYKRDFRRKV CCCCCHHHHHHHHHE | 40.00 | 23749301 | |
623 | Phosphorylation | ERFGEVVTVDLKPDG CCCEEEEEEEECCCC | 17.30 | 22369663 | |
640 | Ubiquitination | IEVTDGNKKEYVELY EEECCCCEEEEEEEH | 53.14 | 23749301 | |
749 | Acetylation | RIPVNGFKDLQGSDG CCCCCCCCCCCCCCC | 59.95 | 24489116 | |
764 | Ubiquitination | PRRFTIEKAGEVQQL CCEEEEEECCCEEEC | 58.82 | 24961812 | |
773 | Ubiquitination | GEVQQLPKSHTCFNR CCEEECCCCCCCCCC | 64.56 | 23749301 | |
793 | Acetylation | YVDYDSMKQKLTLAV CCCHHHHHHHHHHEH | 48.51 | 24489116 |
Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of RSP5_YEAST !! |
Modified Location | Modified Residue | Modification | Function | Reference | ||
---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of RSP5_YEAST !! |
* Distance = the distance between SAP position and PTM sites.
Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of RSP5_YEAST !! |
Kegg Drug | ||||||
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DrugBank | ||||||
There are no disease associations of PTM sites. |
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