RPAB4_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RPAB4_YEAST
• UniProt AC: P40422
• Protein Name: DNA-directed RNA polymerases I, II, and III subunit RPABC4
• Gene Name: RPC10
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 70
• Subcellular Localization: Nucleus, nucleolus .
• Protein Description: DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively. RNA polymerases are composed of mobile elements that move relative to each other. In Pol II, the core element with the central large cleft comprises RPB3, RBP10, RPB11, RPB12 and regions of RPB1 and RPB2 forming the active center..
• Protein Sequence: MSREGFQIPTNLDAAAAGTSQARTATLKYICAECSSKLSLSRTDAVRCKDCGHRILLKARTKRLVQFEAR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSREGFQIP ------CCCCCCCCC	38.75	22369663
10	Phosphorylation	REGFQIPTNLDAAAA CCCCCCCCCHHHHHC	50.47	22369663
19	Phosphorylation	LDAAAAGTSQARTAT HHHHHCCCHHHHHHH	16.97	22369663
20	Phosphorylation	DAAAAGTSQARTATL HHHHCCCHHHHHHHH	22.41	22369663
26	Phosphorylation	TSQARTATLKYICAE CHHHHHHHHHHHHHH	24.14	21440633
28	Ubiquitination	QARTATLKYICAECS HHHHHHHHHHHHHCC	28.37	23749301
28	Acetylation	QARTATLKYICAECS HHHHHHHHHHHHHCC	28.37	24489116
35	Phosphorylation	KYICAECSSKLSLSR HHHHHHCCCCCCCCC	23.24	28889911
36	Phosphorylation	YICAECSSKLSLSRT HHHHHCCCCCCCCCC	49.64	21440633
39	Phosphorylation	AECSSKLSLSRTDAV HHCCCCCCCCCCCCE	28.21	30377154
41	Phosphorylation	CSSKLSLSRTDAVRC CCCCCCCCCCCCEEC	29.74	30377154
43	Phosphorylation	SKLSLSRTDAVRCKD CCCCCCCCCCEECCC	25.57	21440633

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RPAB4_YEAST !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RPAB4_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RPAB4_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RPA2_YEAST	RPA135	physical	10531351
PAN3_YEAST	PAN3	physical	10531351
TFC4_YEAST	TFC4	physical	10559229
RPC2_YEAST	RET1	genetic	10531351
RPB2_YEAST	RPB2	genetic	10531351
RPA2_YEAST	RPA135	genetic	10531351
DED1_YEAST	DED1	genetic	10531351
PABP_YEAST	PAB1	genetic	10531351
PBP2_YEAST	PBP2	genetic	10531351
TFC4_YEAST	TFC4	genetic	10559229
INA22_YEAST	INA22	physical	16554755
MSH2_YEAST	MSH2	physical	16554755
RPA43_YEAST	RPA43	physical	16554755
RPA1_YEAST	RPA190	physical	16554755
RPA2_YEAST	RPA135	physical	16554755
RPAC1_YEAST	RPC40	physical	16554755
SEC23_YEAST	SEC23	genetic	20101242
TCPB_YEAST	CCT2	genetic	20101242
RS5_YEAST	RPS5	genetic	20101242

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"A multidimensional chromatography technology for in-depthphosphoproteome analysis.";
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
Mol. Cell. Proteomics 7:1389-1396(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20, AND MASSSPECTROMETRY.
PubMed: 18407956