dbPTM

ALY2_YEAST - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ALY2_YEAST
UniProt AC	P47029
Protein Name	Arrestin-related trafficking adapter 3
Gene Name	ALY2
Organism	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Sequence Length	1046
Subcellular Localization	Cytoplasm .
Protein Description	May regulate endocytosis by recruiting RSP5 ubiquitin ligase activity to specific plasma membrane proteins in response to extracellular stimuli..
Protein Sequence	MPMDQSISSPLFPMEKDIDIPLDATPLAQSSSLQLFIHLAEPVVFLQGFDPQKTEYPSVVLRGCLVVRILKPTKLKSISLSFKGYSRTEWPEGIPPKRQEFVEIKDIVDHTWALYPPTEQKSKKKMDASAPNESNNAANNFLTKESGASLYRTLSDNETITSRKNSISGLSSLNLSPLGAPGNSSVNVKDRESRQRSRSSSVTSSNGPSRNLSPINLLKRATSPSVSHHNYKPTTTSIFSDLLNNTFTHNDAASHHGHHIPTSSNHLAMTSNNFTSGSGGEFFVFQPGDYIYAFEELIPQAYPESIKADFGFVEYFLFASIERPGAFKSNISARQVVNIVRTQAHNSVEESEPIIISRDWENQLYYDIVIASKDIILDAFLPITFKFAPLDKVTLHRIRIYVTETMEYYCREKKVHRMEPTKKFLLTEQKGPKLPNLPNDANLSKAKNMGNLLQDPKNGDLVNKEYEYQIFIPSRFNNHQQLHPDTSYENIKANHWIKICLRLSRVVDNKRKHYEISIDSPIHVLHRLCSHANTLLPSYDGHPASFPKETDSSISSILESSDDNINLYHNSNIFFPKEVLSSPVLSPNVQPLDILIPHLPSTSLTRNSRQFNRNSKSHPSDNTIFNSAKLKSNIYQPESLQRELASPQAIPLSPITSPMSNMEVPPPDFDFSSDFISDAASGTTTTEVSSSESSILPRDPPSYKDTVLHDNNQKRRPNSKHPTPPSLKASHPNKNSDKNSSETLNKKESMSKIEENKHKRETTPKKRENRDVKSLSTPQREESKDSTSTGNQSNEKNRKRVLSLSSSLHSSPNNSGFAHSALGNLSNESLRSLNRRESVQDNLPSTIRHDNPFFTDLNQVLIEDELKNHDKNELNRHSTNTSSTPASARSSFDYSGINISKDKLNMEPLLSKTETLTNKVNEDSFLRPNDSYVDLLEPSVDTTIDITAPYARNSSAWHPLQNDNDNNQFSPLLGSNENFLNAANAQNSAESDHNNDIFTQGSGLTESSKNSDSEERFISRLSSPEKVLINTLDNESGLQSINESTL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Phosphorylation	--MPMDQSISSPLFP --CCCCCCCCCCCCC	21.85	29136822
8	Phosphorylation	MPMDQSISSPLFPME CCCCCCCCCCCCCCC	31.02	29136822
9	Phosphorylation	PMDQSISSPLFPMEK CCCCCCCCCCCCCCC	24.77	29136822
146	Phosphorylation	NNFLTKESGASLYRT HHCCCCCCCCCCEEE	41.01	19823750
149	Phosphorylation	LTKESGASLYRTLSD CCCCCCCCCEEECCC	29.47	19823750
151	Phosphorylation	KESGASLYRTLSDNE CCCCCCCEEECCCCC	10.08	19823750
153	Phosphorylation	SGASLYRTLSDNETI CCCCCEEECCCCCCC	19.63	22369663
155	Phosphorylation	ASLYRTLSDNETITS CCCEEECCCCCCCCC	37.46	22369663
159	Phosphorylation	RTLSDNETITSRKNS EECCCCCCCCCCCCC	35.49	22369663
161	Phosphorylation	LSDNETITSRKNSIS CCCCCCCCCCCCCCC	29.87	22369663
162	Phosphorylation	SDNETITSRKNSISG CCCCCCCCCCCCCCC	37.09	22369663
164	Ubiquitination	NETITSRKNSISGLS CCCCCCCCCCCCCHH	55.61	24961812
166	Phosphorylation	TITSRKNSISGLSSL CCCCCCCCCCCHHCC	22.59	22369663
168	Phosphorylation	TSRKNSISGLSSLNL CCCCCCCCCHHCCCC	33.24	22369663
171	Phosphorylation	KNSISGLSSLNLSPL CCCCCCHHCCCCCCC	35.98	22369663
172	Phosphorylation	NSISGLSSLNLSPLG CCCCCHHCCCCCCCC	26.73	22369663
176	Phosphorylation	GLSSLNLSPLGAPGN CHHCCCCCCCCCCCC	19.60	22369663
184	Phosphorylation	PLGAPGNSSVNVKDR CCCCCCCCCCCCCCH	41.39	22369663
185	Phosphorylation	LGAPGNSSVNVKDRE CCCCCCCCCCCCCHH	23.01	22369663
189	Ubiquitination	GNSSVNVKDRESRQR CCCCCCCCCHHHHHH	45.95	24961812
197	Phosphorylation	DRESRQRSRSSSVTS CHHHHHHHHHCCCCC	28.33	27017623
199	Phosphorylation	ESRQRSRSSSVTSSN HHHHHHHHCCCCCCC	28.36	23749301
200	Phosphorylation	SRQRSRSSSVTSSNG HHHHHHHCCCCCCCC	27.93	28889911
201	Phosphorylation	RQRSRSSSVTSSNGP HHHHHHCCCCCCCCC	30.57	25752575
204	Phosphorylation	SRSSSVTSSNGPSRN HHHCCCCCCCCCCCC	21.46	23749301
205	Phosphorylation	RSSSVTSSNGPSRNL HHCCCCCCCCCCCCC	36.12	19684113
213	Phosphorylation	NGPSRNLSPINLLKR CCCCCCCCHHHHHHH	27.55	22369663
222	Phosphorylation	INLLKRATSPSVSHH HHHHHHCCCCCCCCC	45.24	19795423
223	Phosphorylation	NLLKRATSPSVSHHN HHHHHCCCCCCCCCC	17.75	19795423
225	Phosphorylation	LKRATSPSVSHHNYK HHHCCCCCCCCCCCC	35.70	19795423
227	Phosphorylation	RATSPSVSHHNYKPT HCCCCCCCCCCCCCC	24.30	19795423
231	Phosphorylation	PSVSHHNYKPTTTSI CCCCCCCCCCCHHHH	17.62	19795423
307	Ubiquitination	QAYPESIKADFGFVE HHCCHHHHCCCCCCH	51.70	17644757
328	Ubiquitination	IERPGAFKSNISARQ CCCCCHHCCCCCHHH	41.90	17644757
392	Ubiquitination	FKFAPLDKVTLHRIR EEECCCCCEEEEEEE	44.81	23749301
430	Ubiquitination	KFLLTEQKGPKLPNL CCEEECCCCCCCCCC	71.58	17644757
433	Ubiquitination	LTEQKGPKLPNLPND EECCCCCCCCCCCCC	82.39	17644757
445	Ubiquitination	PNDANLSKAKNMGNL CCCCCHHHHHHHHHH	67.55	17644757
447	Acetylation	DANLSKAKNMGNLLQ CCCHHHHHHHHHHCC	51.72	24489116
464	Ubiquitination	KNGDLVNKEYEYQIF CCCCCCCCEEEEEEE	54.94	23749301
492	Ubiquitination	DTSYENIKANHWIKI CCCHHHHHHCHHHHH	55.51	17644757
512	Ubiquitination	RVVDNKRKHYEISID HHHCCCCCEEEEECC	52.74	17644757
545	Phosphorylation	SYDGHPASFPKETDS CCCCCCCCCCCCCCC	46.67	28132839
550	Phosphorylation	PASFPKETDSSISSI CCCCCCCCCCCHHHH	47.51	23749301
552	Phosphorylation	SFPKETDSSISSILE CCCCCCCCCHHHHHH	36.67	21551504
553	Phosphorylation	FPKETDSSISSILES CCCCCCCCHHHHHHC	29.33	21440633
555	Phosphorylation	KETDSSISSILESSD CCCCCCHHHHHHCCC	17.18	21440633
556	Phosphorylation	ETDSSISSILESSDD CCCCCHHHHHHCCCC	29.53	23749301
577	Ubiquitination	NSNIFFPKEVLSSPV CCCEECCHHHHCCCC	56.13	17644757
581	Phosphorylation	FFPKEVLSSPVLSPN ECCHHHHCCCCCCCC	37.46	22369663
582	Phosphorylation	FPKEVLSSPVLSPNV CCHHHHCCCCCCCCC	18.18	22369663
586	Phosphorylation	VLSSPVLSPNVQPLD HHCCCCCCCCCCCCC	18.05	22369663
601	Phosphorylation	ILIPHLPSTSLTRNS EEECCCCCCCCCCCC	36.67	23749301
602	Phosphorylation	LIPHLPSTSLTRNSR EECCCCCCCCCCCCC	26.30	22369663
603	Phosphorylation	IPHLPSTSLTRNSRQ ECCCCCCCCCCCCCC	31.89	21440633
605	Phosphorylation	HLPSTSLTRNSRQFN CCCCCCCCCCCCCCC	27.80	23749301
608	Phosphorylation	STSLTRNSRQFNRNS CCCCCCCCCCCCCCC	24.94	21440633
615	Phosphorylation	SRQFNRNSKSHPSDN CCCCCCCCCCCCCCC	32.09	21440633
616	Ubiquitination	RQFNRNSKSHPSDNT CCCCCCCCCCCCCCC	57.23	23749301
617	Phosphorylation	QFNRNSKSHPSDNTI CCCCCCCCCCCCCCC	40.21	22369663
620	Phosphorylation	RNSKSHPSDNTIFNS CCCCCCCCCCCCCCC	37.76	22369663
629	Ubiquitination	NTIFNSAKLKSNIYQ CCCCCCHHHHCCCCC	57.16	23749301
631	Acetylation	IFNSAKLKSNIYQPE CCCCHHHHCCCCCCH	40.53	25381059
631	Ubiquitination	IFNSAKLKSNIYQPE CCCCHHHHCCCCCCH	40.53	23749301
723	Phosphorylation	RPNSKHPTPPSLKAS CCCCCCCCCCCCCCC	47.77	20377248
726	Phosphorylation	SKHPTPPSLKASHPN CCCCCCCCCCCCCCC	43.64	29136822
740	Phosphorylation	NKNSDKNSSETLNKK CCCCCCCHHHHHCHH	34.98	28889911
741	Phosphorylation	KNSDKNSSETLNKKE CCCCCCHHHHHCHHH	43.95	28889911
773	Ubiquitination	KRENRDVKSLSTPQR HHCCCCCCCCCCCCC	50.39	23749301
774	Phosphorylation	RENRDVKSLSTPQRE HCCCCCCCCCCCCCH	27.66	21551504
776	Phosphorylation	NRDVKSLSTPQREES CCCCCCCCCCCCHHH	45.17	19779198
777	Phosphorylation	RDVKSLSTPQREESK CCCCCCCCCCCHHHC	28.83	25521595
783	Phosphorylation	STPQREESKDSTSTG CCCCCHHHCCCCCCC	36.69	19779198
786	Phosphorylation	QREESKDSTSTGNQS CCHHHCCCCCCCCCC	28.17	19779198
788	Phosphorylation	EESKDSTSTGNQSNE HHHCCCCCCCCCCCH	37.96	27017623
789	Phosphorylation	ESKDSTSTGNQSNEK HHCCCCCCCCCCCHH	39.99	19779198
793	Phosphorylation	STSTGNQSNEKNRKR CCCCCCCCCHHHHHH	51.46	28889911
803	Phosphorylation	KNRKRVLSLSSSLHS HHHHHHHHHHHHHCC	24.29	19779198
805	Phosphorylation	RKRVLSLSSSLHSSP HHHHHHHHHHHCCCC	17.95	19779198
807	Phosphorylation	RVLSLSSSLHSSPNN HHHHHHHHHCCCCCC	27.07	23749301
810	Phosphorylation	SLSSSLHSSPNNSGF HHHHHHCCCCCCCCC	53.43	23749301
811	Phosphorylation	LSSSLHSSPNNSGFA HHHHHCCCCCCCCCC	22.39	19779198
815	Phosphorylation	LHSSPNNSGFAHSAL HCCCCCCCCCCCHHH	41.76	19779198
820	Phosphorylation	NNSGFAHSALGNLSN CCCCCCCHHHHCCCH	23.04	28889911
826	Phosphorylation	HSALGNLSNESLRSL CHHHHCCCHHHHHHH	42.42	25752575
829	Phosphorylation	LGNLSNESLRSLNRR HHCCCHHHHHHHCCH	33.08	23749301
838	Phosphorylation	RSLNRRESVQDNLPS HHHCCHHHHHHCCCH	24.22	17330950
878	Phosphorylation	KNELNRHSTNTSSTP CCCHHHCCCCCCCCC	21.81	23749301
879	Phosphorylation	NELNRHSTNTSSTPA CCHHHCCCCCCCCCC	36.07	28889911
881	Phosphorylation	LNRHSTNTSSTPASA HHHCCCCCCCCCCHH	24.92	28889911
882	Phosphorylation	NRHSTNTSSTPASAR HHCCCCCCCCCCHHH	33.99	17563356
883	Phosphorylation	RHSTNTSSTPASARS HCCCCCCCCCCHHHH	36.05	17563356
884	Phosphorylation	HSTNTSSTPASARSS CCCCCCCCCCHHHHC	23.73	17563356
887	Phosphorylation	NTSSTPASARSSFDY CCCCCCCHHHHCCCC	25.97	28889911
890	Phosphorylation	STPASARSSFDYSGI CCCCHHHHCCCCCCC	34.76	22369663
891	Phosphorylation	TPASARSSFDYSGIN CCCHHHHCCCCCCCC	19.31	22369663
894	Phosphorylation	SARSSFDYSGINISK HHHHCCCCCCCCCCC	13.54	19779198
895	Phosphorylation	ARSSFDYSGINISKD HHHCCCCCCCCCCCC	34.73	28889911
900	Phosphorylation	DYSGINISKDKLNME CCCCCCCCCCCCCCH	30.25	22369663
903	Ubiquitination	GINISKDKLNMEPLL CCCCCCCCCCCHHHC	45.56	23749301
911	Phosphorylation	LNMEPLLSKTETLTN CCCHHHCCCHHHHCC	45.26	22369663
912	Ubiquitination	NMEPLLSKTETLTNK CCHHHCCCHHHHCCC	50.80	24961812
913	Phosphorylation	MEPLLSKTETLTNKV CHHHCCCHHHHCCCC	30.72	21440633
915	Phosphorylation	PLLSKTETLTNKVNE HHCCCHHHHCCCCCC	44.08	25752575
919	Ubiquitination	KTETLTNKVNEDSFL CHHHHCCCCCCCCCC	41.00	24961812
924	Phosphorylation	TNKVNEDSFLRPNDS CCCCCCCCCCCCCCC	21.72	23749301
931	Phosphorylation	SFLRPNDSYVDLLEP CCCCCCCCCCCCCCC	33.42	21440633
932	Phosphorylation	FLRPNDSYVDLLEPS CCCCCCCCCCCCCCC	10.80	23749301
1019	Phosphorylation	DSEERFISRLSSPEK CHHHHHHHHHCCCCE	25.18	21440633
1022	Phosphorylation	ERFISRLSSPEKVLI HHHHHHHCCCCEEEE	42.61	22369663
1023	Phosphorylation	RFISRLSSPEKVLIN HHHHHHCCCCEEEEE	41.49	22369663
1036	Phosphorylation	INTLDNESGLQSINE EECCCCCCHHHHHHH	51.57	28152593
1040	Phosphorylation	DNESGLQSINESTL- CCCCHHHHHHHCCC-	32.21	28152593
1044	Phosphorylation	GLQSINESTL----- HHHHHHHCCC-----	30.44	19779198
1045	Phosphorylation	LQSINESTL------ HHHHHHCCC------	30.24	21440633

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	RSP5	P39940	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ALY2_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ALY2_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AP2A_YEAST	APL3	physical	16554755
RV167_YEAST	RVS167	physical	16554755
RSP5_YEAST	RSP5	physical	16554755
AP2B_YEAST	APL1	physical	16554755
HSP71_YEAST	SSA1	physical	19536198
GAP1_YEAST	GAP1	physical	20739461
LST4_YEAST	LST4	genetic	20739461
CLH_YEAST	CHC1	physical	20739461
AP2B_YEAST	APL1	physical	20739461
AP1B1_YEAST	APL2	physical	20739461
AP1B1_YEAST	APL2	genetic	20739461
TAT1_YEAST	TAT1	genetic	21908639
RSP5_YEAST	RSP5	physical	23824189
BMH1_YEAST	BMH1	physical	23824189
BMH2_YEAST	BMH2	physical	23824189
RHO1_YEAST	RHO1	physical	26459639
LDB19_YEAST	LDB19	genetic	26459639
THRC_YEAST	THR4	genetic	27708008
RM01_YEAST	MRPL1	genetic	27708008

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ALY2_YEAST

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9; SER-155; SER-162;SER-166; SER-168; SER-201; SER-213; SER-820; SER-826; SER-838;SER-890; SER-891; SER-900; SER-1022; SER-1023 AND SER-1036, AND MASSSPECTROMETRY.	18407956 [Abstract]
"Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166; SER-172; SER-838;SER-882; SER-883; THR-884; SER-1022 AND SER-1023, AND MASSSPECTROMETRY.	17563356 [Abstract]
"Large-scale phosphorylation analysis of alpha-factor-arrestedSaccharomyces cerevisiae."; Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,Elias J.E., Gygi S.P.; J. Proteome Res. 6:1190-1197(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-162 ANDSER-838, AND MASS SPECTROMETRY.	17330950 [Abstract]
"Quantitative phosphoproteomics applied to the yeast pheromonesignaling pathway."; Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J.,Mann M., Jensen O.N.; Mol. Cell. Proteomics 4:310-327(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166 AND SER-213, ANDMASS SPECTROMETRY.	15665377 [Abstract]
"A proteomics approach to understanding protein ubiquitination."; Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D.,Marsischky G., Roelofs J., Finley D., Gygi S.P.; Nat. Biotechnol. 21:921-926(2003). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-900, AND MASSSPECTROMETRY.	12872131 [Abstract]