| UniProt ID | SYA_YEAST | |
|---|---|---|
| UniProt AC | P40825 | |
| Protein Name | Alanine--tRNA ligase, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03133} | |
| Gene Name | ALA1 {ECO:0000255|HAMAP-Rule:MF_03133} | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 983 | |
| Subcellular Localization |
Isoform Cytoplasmic: Cytoplasm. Isoform Mitochondrial: Mitochondrion. |
|
| Protein Description | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.. | |
| Protein Sequence | MTSTTGLRNLTLSFKKQLTTSTRTIMTIGDKQKWTATNVRNTFLDYFKSKEHKFVKSSPVVPFDDPTLLFANAGMNQYKPIFLGTVDPASDFYTLKRAYNSQKCIRAGGKHNDLEDVGKDSYHHTFFEMLGNWSFGDYFKKEAITYSWTLLTEVYGIPKDRLYVTYFEGDEKLGLEPDTEARELWKNVGVPDDHILPGNAKDNFWEMGDQGPCGPCSEIHYDRIGGRNAASLVNMDDPDVLEVWNLVFIQFNREQDGSLKPLPAKHIDTGMGFERLVSVLQDVRSNYDTDVFTPLFERIQEITSVRPYSGNFGENDKDGIDTAYRVLADHVRTLTFALADGGVPNNEGRGYVLRRILRRGARYARKYMNYPIGNFFSTLAPTLISQVQDIFPELAKDPAFLFEILDEEEASFAKTLDRGERLFEKYASAASKTESKTLDGKQVWRLYDTYGFPVDLTELMAEEQGLKIDGPGFEKAKQESYEASKRGGKKDQSDLIKLNVHELSELNDAKVPKTNDEFKYGSANVEGTILKLHDGTNFVDEITEPGKKYGIILDKTCFYAEQGGQEYDTGKIVIDDAAEFNVENVQLYNGFVFHTGSLEEGKLSVGDKIIASFDELRRFPIKNNHTGTHILNFALKETLGNDVDQKGSLVAPEKLRFDFSHKKAVSNEELKKVEDICNEQIKENLQVFYKEIPLDLAKSIDGVRAVFGETYPDPVRVVSVGKPIEELLANPANEEWTKYSIEFCGGTHVNKTGDIKYFVILEESGIAKGIRRIVAVTGTEAFEAQRLAEQFAADLDAADKLPFSPIKEKKLKELGVKLGQLSISVITKNELKQKFNKIEKAVKDEVKSRAKKENKQTLDEVKTFFETNENAPYLVKFIDISPNAKAITEAINYMKSNDSVKDKSIYLLAGNDPEGRVAHGCYISNAALAKGIDGSALAKKVSSIIGGKAGGKGNVFQGMGDKPAAIKDAVDDLESLFKEKLSI | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 5 | Phosphorylation | ---MTSTTGLRNLTL ---CCCCCCCCCCEE | 33.70 | 30377154 | |
| 11 | Phosphorylation | TTGLRNLTLSFKKQL CCCCCCCEEEEHHCC | 24.73 | 30377154 | |
| 13 | Phosphorylation | GLRNLTLSFKKQLTT CCCCCEEEEHHCCCC | 29.77 | 30377154 | |
| 48 | Acetylation | NTFLDYFKSKEHKFV HHHHHHHHCCCCCCC | 55.15 | 24489116 | |
| 57 | Phosphorylation | KEHKFVKSSPVVPFD CCCCCCCCCCCCCCC | 34.27 | 21551504 | |
| 78 | Ubiquitination | ANAGMNQYKPIFLGT ECCCCCCCCCEEEEE | 17.46 | 23749301 | |
| 103 | Ubiquitination | KRAYNSQKCIRAGGK HHHHHCHHCHHCCCC | 31.30 | - | |
| 110 | Acetylation | KCIRAGGKHNDLEDV HCHHCCCCCCCHHHH | 38.01 | 22865919 | |
| 145 | Phosphorylation | YFKKEAITYSWTLLT HHHHHHHHEEEEHHH | 20.67 | 21082442 | |
| 146 | Phosphorylation | FKKEAITYSWTLLTE HHHHHHHEEEEHHHH | 8.97 | 28889911 | |
| 147 | Phosphorylation | KKEAITYSWTLLTEV HHHHHHEEEEHHHHH | 12.63 | 21082442 | |
| 147 | Ubiquitination | KKEAITYSWTLLTEV HHHHHHEEEEHHHHH | 12.63 | 15699485 | |
| 149 | Phosphorylation | EAITYSWTLLTEVYG HHHHEEEEHHHHHHC | 13.12 | 28889911 | |
| 172 | Acetylation | TYFEGDEKLGLEPDT EEEECCHHCCCCCCH | 53.23 | 24489116 | |
| 186 | Acetylation | TEARELWKNVGVPDD HHHHHHHHHCCCCCC | 55.14 | 24489116 | |
| 265 | Acetylation | SLKPLPAKHIDTGMG CCCCCCCCCCCCCCC | 38.87 | 24489116 | |
| 285 | Phosphorylation | SVLQDVRSNYDTDVF HHHHHHHHCCCCCCC | 39.32 | 19779198 | |
| 287 | Phosphorylation | LQDVRSNYDTDVFTP HHHHHHCCCCCCCHH | 22.82 | 19779198 | |
| 293 | Phosphorylation | NYDTDVFTPLFERIQ CCCCCCCHHHHHHHH | 20.94 | 27214570 | |
| 317 | Acetylation | GNFGENDKDGIDTAY CCCCCCCCCCHHHHH | 70.23 | 24489116 | |
| 425 | Acetylation | RGERLFEKYASAASK HHHHHHHHHHHHCCC | 38.49 | 24489116 | |
| 475 | Acetylation | IDGPGFEKAKQESYE CCCCCHHHHHHHHHH | 59.63 | 22865919 | |
| 479 | Phosphorylation | GFEKAKQESYEASKR CHHHHHHHHHHHHHC | 55.36 | 18407956 | |
| 484 | Phosphorylation | KQESYEASKRGGKKD HHHHHHHHHCCCCCC | 15.79 | 28889911 | |
| 485 | Ubiquitination | QESYEASKRGGKKDQ HHHHHHHHCCCCCCH | 63.21 | 23749301 | |
| 497 | Acetylation | KDQSDLIKLNVHELS CCHHHHHCCCHHHHH | 41.11 | 24489116 | |
| 504 | Phosphorylation | KLNVHELSELNDAKV CCCHHHHHHHCCCCC | 36.73 | 28889911 | |
| 510 | Acetylation | LSELNDAKVPKTNDE HHHHCCCCCCCCCCC | 63.03 | 24489116 | |
| 510 | Ubiquitination | LSELNDAKVPKTNDE HHHHCCCCCCCCCCC | 63.03 | - | |
| 513 | Acetylation | LNDAKVPKTNDEFKY HCCCCCCCCCCCCCC | 64.05 | 24489116 | |
| 519 | Acetylation | PKTNDEFKYGSANVE CCCCCCCCCCCCCEE | 46.80 | 24489116 | |
| 522 | Phosphorylation | NDEFKYGSANVEGTI CCCCCCCCCCEEEEE | 17.36 | 24961812 | |
| 531 | Acetylation | NVEGTILKLHDGTNF CEEEEEEEECCCCCC | 39.67 | 24489116 | |
| 548 | Acetylation | EITEPGKKYGIILDK ECCCCCCEEEEEEEC | 55.41 | 24489116 | |
| 608 | Acetylation | GKLSVGDKIIASFDE CCCCCCCEEEEEHHH | 30.04 | 24489116 | |
| 621 | Ubiquitination | DELRRFPIKNNHTGT HHHHCCCCCCCCCCH | 7.39 | 23749301 | |
| 629 | Ubiquitination | KNNHTGTHILNFALK CCCCCCHHHHHHHHH | 24.71 | 23749301 | |
| 646 | Ubiquitination | LGNDVDQKGSLVAPE HCCCCCCCCCCCCCC | 46.10 | - | |
| 646 | Acetylation | LGNDVDQKGSLVAPE HCCCCCCCCCCCCCC | 46.10 | 24489116 | |
| 646 | Succinylation | LGNDVDQKGSLVAPE HCCCCCCCCCCCCCC | 46.10 | 23954790 | |
| 654 | Ubiquitination | GSLVAPEKLRFDFSH CCCCCCCHHCCCCCC | 43.85 | - | |
| 654 | Acetylation | GSLVAPEKLRFDFSH CCCCCCCHHCCCCCC | 43.85 | 24489116 | |
| 665 | Ubiquitination | DFSHKKAVSNEELKK CCCCCCCCCHHHHHH | 9.67 | 24961812 | |
| 666 | Phosphorylation | FSHKKAVSNEELKKV CCCCCCCCHHHHHHH | 43.30 | 30377154 | |
| 673 | Ubiquitination | SNEELKKVEDICNEQ CHHHHHHHHHHHHHH | 8.37 | 23749301 | |
| 682 | Acetylation | DICNEQIKENLQVFY HHHHHHHHHHHHHHH | 40.00 | 24489116 | |
| 690 | Acetylation | ENLQVFYKEIPLDLA HHHHHHHHHCCHHHH | 37.39 | 24489116 | |
| 698 | Acetylation | EIPLDLAKSIDGVRA HCCHHHHHHCCCCCH | 56.06 | 24489116 | |
| 698 | Ubiquitination | EIPLDLAKSIDGVRA HCCHHHHHHCCCCCH | 56.06 | - | |
| 710 | Phosphorylation | VRAVFGETYPDPVRV CCHHHCCCCCCCEEE | 40.51 | 22369663 | |
| 711 | Phosphorylation | RAVFGETYPDPVRVV CHHHCCCCCCCEEEE | 11.01 | 22369663 | |
| 756 | Acetylation | VNKTGDIKYFVILEE ECCCCCEEEEEEEEC | 37.06 | 24489116 | |
| 800 | Acetylation | ADLDAADKLPFSPIK CCCCHHHCCCCCCCC | 54.02 | 24489116 | |
| 804 | Phosphorylation | AADKLPFSPIKEKKL HHHCCCCCCCCHHHH | 24.63 | 21440633 | |
| 822 | Phosphorylation | GVKLGQLSISVITKN CCCHHCCEEEEEEHH | 12.63 | 22369663 | |
| 824 | Phosphorylation | KLGQLSISVITKNEL CHHCCEEEEEEHHHH | 12.23 | 22369663 | |
| 827 | Phosphorylation | QLSISVITKNELKQK CCEEEEEEHHHHHHH | 26.17 | 22369663 | |
| 843 | Acetylation | NKIEKAVKDEVKSRA HHHHHHHHHHHHHHH | 53.70 | 24489116 | |
| 857 | Phosphorylation | AKKENKQTLDEVKTF HHHHHCCCHHHHHHH | 37.47 | 19684113 | |
| 862 | Acetylation | KQTLDEVKTFFETNE CCCHHHHHHHHHCCC | 37.25 | 24489116 | |
| 863 | Phosphorylation | QTLDEVKTFFETNEN CCHHHHHHHHHCCCC | 38.44 | 22369663 | |
| 867 | Phosphorylation | EVKTFFETNENAPYL HHHHHHHCCCCCCEE | 42.55 | 22369663 | |
| 873 | Phosphorylation | ETNENAPYLVKFIDI HCCCCCCEEEEEEEC | 23.01 | 22369663 | |
| 879 | Phosphorylation | PYLVKFIDISPNAKA CEEEEEEECCCCHHH | 38.14 | 19795423 | |
| 881 | Phosphorylation | LVKFIDISPNAKAIT EEEEEECCCCHHHHH | 14.35 | 21440633 | |
| 885 | Acetylation | IDISPNAKAITEAIN EECCCCHHHHHHHHH | 47.14 | 24489116 | |
| 895 | Acetylation | TEAINYMKSNDSVKD HHHHHHHHCCCCCCC | 35.43 | 24489116 | |
| 899 | Phosphorylation | NYMKSNDSVKDKSIY HHHHCCCCCCCCEEE | 35.00 | 30377154 | |
| 901 | Succinylation | MKSNDSVKDKSIYLL HHCCCCCCCCEEEEE | 64.49 | 23954790 | |
| 903 | Acetylation | SNDSVKDKSIYLLAG CCCCCCCCEEEEEEC | 32.79 | 24489116 | |
| 904 | Phosphorylation | NDSVKDKSIYLLAGN CCCCCCCEEEEEECC | 26.97 | 19795423 | |
| 905 | Ubiquitination | DSVKDKSIYLLAGND CCCCCCEEEEEECCC | 3.28 | 17644757 | |
| 906 | Phosphorylation | SVKDKSIYLLAGNDP CCCCCEEEEEECCCC | 11.62 | 19823750 | |
| 924 | Phosphorylation | VAHGCYISNAALAKG CCCCEEECHHHHHCC | 8.24 | 30377154 | |
| 930 | Acetylation | ISNAALAKGIDGSAL ECHHHHHCCCCHHHH | 58.19 | 24489116 | |
| 939 | Succinylation | IDGSALAKKVSSIIG CCHHHHHHHHHHHHC | 55.33 | 23954790 | |
| 942 | Phosphorylation | SALAKKVSSIIGGKA HHHHHHHHHHHCCCC | 25.38 | 21440633 | |
| 943 | Phosphorylation | ALAKKVSSIIGGKAG HHHHHHHHHHCCCCC | 22.63 | 23749301 | |
| 950 | Phosphorylation | SIIGGKAGGKGNVFQ HHHCCCCCCCCCCCC | 41.66 | 19779198 | |
| 975 | Phosphorylation | DAVDDLESLFKEKLS HHHHHHHHHHHHHHC | 46.53 | 22369663 | |
| 978 | Acetylation | DDLESLFKEKLSI-- HHHHHHHHHHHCC-- | 60.27 | 24489116 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of SYA_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of SYA_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of SYA_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-504 AND SER-975, ANDMASS SPECTROMETRY. | |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-975, AND MASSSPECTROMETRY. | |
