PFKA2_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PFKA2_YEAST
• UniProt AC: P16862
• Protein Name: ATP-dependent 6-phosphofructokinase subunit beta {ECO:0000255|HAMAP-Rule:MF_03184}
• Gene Name: PFK2
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 959
• Subcellular Localization: Cytoplasm . Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side .
• Protein Description: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis..
• Protein Sequence: MTVTTPFVNGTSYCTVTAYSVQSYKAAIDFYTKFLSLENRSSPDENSTLLSNDSISLKILLRPDEKINKNVEAHLKELNSITKTQDWRSHATQSLVFNTSDILAVKDTLNAMNAPLQGYPTELFPMQLYTLDPLGNVVGVTSTKNAVSTKPTPPPAPEASAESGLSSKVHSYTDLAYRMKTTDTYPSLPKPLNRPQKAIAVMTSGGDAPGMNSNVRAIVRSAIFKGCRAFVVMEGYEGLVRGGPEYIKEFHWEDVRGWSAEGGTNIGTARCMEFKKREGRLLGAQHLIEAGVDALIVCGGDGSLTGADLFRSEWPSLIEELLKTNRISNEQYERMKHLNICGTVGSIDNDMSTTDATIGAYSALDRICKAIDYVEATANSHSRAFVVEVMGRNCGWLALLAGIATSADYIFIPEKPATSSEWQDQMCDIVSKHRSRGKRTTIVVVAEGAIAADLTPISPSDVHKVLVDRLGLDTRITTLGHVQRGGTAVAYDRILATLQGLEAVNAVLESTPDTPSPLIAVNENKIVRKPLMESVKLTKAVAEAIQAKDFKRAMSLRDTEFIEHLNNFMAINSADHNEPKLPKDKRLKIAIVNVGAPAGGINSAVYSMATYCMSQGHRPYAIYNGWSGLARHESVRSLNWKDMLGWQSRGGSEIGTNRVTPEEADLGMIAYYFQKYEFDGLIIVGGFEAFESLHQLERARESYPAFRIPMVLIPATLSNNVPGTEYSLGSDTALNALMEYCDVVKQSASSTRGRAFVVDCQGGNSGYLATYASLAVGAQVSYVPEEGISLEQLSEDIEYLAQSFEKAEGRGRFGKLILKSTNASKALSATKLAEVITAEADGRFDAKPAYPGHVQQGGLPSPIDRTRATRMAIKAVGFIKDNQAAIAEARAAEENFNADDKTISDTAAVVGVKGSHVVYNSIRQLYDYETEVSMRMPKVIHWQATRLIADHLVGRKRVD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
13	Phosphorylation	PFVNGTSYCTVTAYS CCCCCCCEEEEEEEE		23749301
15	Phosphorylation	VNGTSYCTVTAYSVQ CCCCCEEEEEEEECC		23749301
19	Phosphorylation	SYCTVTAYSVQSYKA CEEEEEEEECCHHHH		23749301
20	Phosphorylation	YCTVTAYSVQSYKAA EEEEEEEECCHHHHH		28889911
23	Phosphorylation	VTAYSVQSYKAAIDF EEEEECCHHHHHHHH		23749301
31	Phosphorylation	YKAAIDFYTKFLSLE HHHHHHHHHHHHCCC		28889911
32	Phosphorylation	KAAIDFYTKFLSLEN HHHHHHHHHHHCCCC		28889911
33	Ubiquitination	AAIDFYTKFLSLENR HHHHHHHHHHCCCCC		23749301
33	Acetylation	AAIDFYTKFLSLENR HHHHHHHHHHCCCCC		24489116
36	Phosphorylation	DFYTKFLSLENRSSP HHHHHHHCCCCCCCC		22369663
41	Phosphorylation	FLSLENRSSPDENST HHCCCCCCCCCCCCC		22369663
42	Phosphorylation	LSLENRSSPDENSTL HCCCCCCCCCCCCCC		22369663
47	Phosphorylation	RSSPDENSTLLSNDS CCCCCCCCCCCCCCC		22369663
48	Phosphorylation	SSPDENSTLLSNDSI CCCCCCCCCCCCCCE		22369663
51	Phosphorylation	DENSTLLSNDSISLK CCCCCCCCCCCEEEE		22369663
54	Phosphorylation	STLLSNDSISLKILL CCCCCCCCEEEEEEE		22369663
56	Phosphorylation	LLSNDSISLKILLRP CCCCCCEEEEEEECC		22369663
66	Acetylation	ILLRPDEKINKNVEA EEECCCHHHCHHHHH		24489116
66	Ubiquitination	ILLRPDEKINKNVEA EEECCCHHHCHHHHH		22817900
69	Ubiquitination	RPDEKINKNVEAHLK CCCHHHCHHHHHHHH		22817900
69	Acetylation	RPDEKINKNVEAHLK CCCHHHCHHHHHHHH		24489116
76	Acetylation	KNVEAHLKELNSITK HHHHHHHHHHHCCCC		24489116
80	Phosphorylation	AHLKELNSITKTQDW HHHHHHHCCCCCCHH		28889911
82	Phosphorylation	LKELNSITKTQDWRS HHHHHCCCCCCHHHH		28889911
83	Acetylation	KELNSITKTQDWRSH HHHHCCCCCCHHHHH		24489116
144	Ubiquitination	VVGVTSTKNAVSTKP EEEEECCCCCCCCCC		17644757
148	Phosphorylation	TSTKNAVSTKPTPPP ECCCCCCCCCCCCCC		22890988
149	Phosphorylation	STKNAVSTKPTPPPA CCCCCCCCCCCCCCC		22890988
150	Ubiquitination	TKNAVSTKPTPPPAP CCCCCCCCCCCCCCC		23749301
152	Phosphorylation	NAVSTKPTPPPAPEA CCCCCCCCCCCCCCC		22369663
160	Phosphorylation	PPPAPEASAESGLSS CCCCCCCHHHCCCCC		25521595
163	Phosphorylation	APEASAESGLSSKVH CCCCHHHCCCCCHHE		22369663
166	Phosphorylation	ASAESGLSSKVHSYT CHHHCCCCCHHEECC		22369663
167	Phosphorylation	SAESGLSSKVHSYTD HHHCCCCCHHEECCC		22369663
168	Acetylation	AESGLSSKVHSYTDL HHCCCCCHHEECCCC		24489116
168	Ubiquitination	AESGLSSKVHSYTDL HHCCCCCHHEECCCC		17644757
171	Phosphorylation	GLSSKVHSYTDLAYR CCCCHHEECCCCCHH		22369663
172	Phosphorylation	LSSKVHSYTDLAYRM CCCHHEECCCCCHHC		25521595
173	Phosphorylation	SSKVHSYTDLAYRMK CCHHEECCCCCHHCC		22369663
177	Phosphorylation	HSYTDLAYRMKTTDT EECCCCCHHCCCCCC		22890988
180	Methylation	TDLAYRMKTTDTYPS CCCCHHCCCCCCCCC		23161681
180	Ubiquitination	TDLAYRMKTTDTYPS CCCCHHCCCCCCCCC		17287358
180	Succinylation	TDLAYRMKTTDTYPS CCCCHHCCCCCCCCC		23954790
181	Phosphorylation	DLAYRMKTTDTYPSL CCCHHCCCCCCCCCC		22369663
182	Phosphorylation	LAYRMKTTDTYPSLP CCHHCCCCCCCCCCC		22369663
184	Phosphorylation	YRMKTTDTYPSLPKP HHCCCCCCCCCCCCC		22369663
185	Phosphorylation	RMKTTDTYPSLPKPL HCCCCCCCCCCCCCC		28889911
187	Phosphorylation	KTTDTYPSLPKPLNR CCCCCCCCCCCCCCC		22369663
190	Acetylation	DTYPSLPKPLNRPQK CCCCCCCCCCCCCCC		24489116
203	Phosphorylation	QKAIAVMTSGGDAPG CCEEEEEECCCCCCC		28889911
204	Phosphorylation	KAIAVMTSGGDAPGM CEEEEEECCCCCCCC		28889911
213	Phosphorylation	GDAPGMNSNVRAIVR CCCCCCCHHHHHHHH		28889911
248	Ubiquitination	RGGPEYIKEFHWEDV CCCHHHHHEECHHHC		24961812
248	Acetylation	RGGPEYIKEFHWEDV CCCHHHHHEECHHHC		24489116
323	Acetylation	SLIEELLKTNRISNE HHHHHHHHHCCCCHH		24489116
343	Phosphorylation	KHLNICGTVGSIDND HHCCCCCCCCCCCCC		19779198
346	Phosphorylation	NICGTVGSIDNDMST CCCCCCCCCCCCCCC		19779198
354	Phosphorylation	IDNDMSTTDATIGAY CCCCCCCCHHHHHHH		19779198
369	Acetylation	SALDRICKAIDYVEA HHHHHHHHHHCHHHH		24489116
369	Ubiquitination	SALDRICKAIDYVEA HHHHHHHHHHCHHHH		23749301
373	Phosphorylation	RICKAIDYVEATANS HHHHHHCHHHHHCCC		19779198
380	Phosphorylation	YVEATANSHSRAFVV HHHHHCCCCCCEEEE		28889911
477	Phosphorylation	LGLDTRITTLGHVQR CCCCCEEEEEECHHC		28889911
514	Phosphorylation	VLESTPDTPSPLIAV HHHCCCCCCCCEEEE		28889911
529	Ubiquitination	NENKIVRKPLMESVK CCCCCCCCHHHHHHH		23749301
536	Ubiquitination	KPLMESVKLTKAVAE CHHHHHHHHHHHHHH		23749301
539	Acetylation	MESVKLTKAVAEAIQ HHHHHHHHHHHHHHH		22865919
539	Ubiquitination	MESVKLTKAVAEAIQ HHHHHHHHHHHHHHH		23749301
548	Acetylation	VAEAIQAKDFKRAMS HHHHHHHHHHHHHHC		24489116
548	Ubiquitination	VAEAIQAKDFKRAMS HHHHHHHHHHHHHHC		23749301
555	Phosphorylation	KDFKRAMSLRDTEFI HHHHHHHCCCCHHHH		28889911
559	Phosphorylation	RAMSLRDTEFIEHLN HHHCCCCHHHHHHHH		19779198
580	Acetylation	SADHNEPKLPKDKRL CCCCCCCCCCCCCCC		24489116
637	Phosphorylation	ARHESVRSLNWKDML HCCCCHHCCCHHHHH		21440633
641	Ubiquitination	SVRSLNWKDMLGWQS CHHCCCHHHHHCCCC		23749301
641	Acetylation	SVRSLNWKDMLGWQS CHHCCCHHHHHCCCC		24489116
652	Phosphorylation	GWQSRGGSEIGTNRV CCCCCCCCCCCCCCC		28889911
803	Phosphorylation	DIEYLAQSFEKAEGR HHHHHHHHHHHHHCC		28889911
815	Ubiquitination	EGRGRFGKLILKSTN HCCCCHHHHHHHCCC		23749301
815	Acetylation	EGRGRFGKLILKSTN HCCCCHHHHHHHCCC		24489116
819	Ubiquitination	RFGKLILKSTNASKA CHHHHHHHCCCHHHH		23749301
819	Acetylation	RFGKLILKSTNASKA CHHHHHHHCCCHHHH		25381059
820	Phosphorylation	FGKLILKSTNASKAL HHHHHHHCCCHHHHC		19795423
821	Phosphorylation	GKLILKSTNASKALS HHHHHHCCCHHHHCC		19795423
825	Ubiquitination	LKSTNASKALSATKL HHCCCHHHHCCHHHH		23749301
831	Ubiquitination	SKALSATKLAEVITA HHHCCHHHHHHHHHH		23749301
831	Acetylation	SKALSATKLAEVITA HHHCCHHHHHHHHHH		24489116
847	Acetylation	ADGRFDAKPAYPGHV CCCCCCCCCCCCCCC		24489116
847	Ubiquitination	ADGRFDAKPAYPGHV CCCCCCCCCCCCCCC		23749301
861	Phosphorylation	VQQGGLPSPIDRTRA CCCCCCCCCCCHHHH		30377154
901	Acetylation	ENFNADDKTISDTAA HHCCCCCCCHHCCEE		24489116
902	Phosphorylation	NFNADDKTISDTAAV HCCCCCCCHHCCEEE		19779198
904	Phosphorylation	NADDKTISDTAAVVG CCCCCCHHCCEEEEE		19779198
906	Phosphorylation	DDKTISDTAAVVGVK CCCCHHCCEEEEECC		23749301
913	Ubiquitination	TAAVVGVKGSHVVYN CEEEEECCCHHHHHH		24961812
915	Phosphorylation	AVVGVKGSHVVYNSI EEEECCCHHHHHHCH		21440633
938	Acetylation	EVSMRMPKVIHWQAT HHHHCCCCCHHHHHH		24489116
938	Ubiquitination	EVSMRMPKVIHWQAT HHHHCCCCCHHHHHH		23749301

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PFKA2_YEAST !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PFKA2_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PFKA2_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PFKA1_YEAST	PFK1	physical	11805826
IF4A_YEAST	TIF2	physical	11805837
PFKA1_YEAST	PFK1	physical	11805837
RS10A_YEAST	RPS10A	physical	16429126
RS22A_YEAST	RPS22A	physical	16429126
PFKA1_YEAST	PFK1	physical	16429126
IPK1_YEAST	IPK1	genetic	19269370
BAS1_YEAST	BAS1	genetic	19269370
ELO3_YEAST	ELO3	genetic	22808036
PFKA1_YEAST	PFK1	genetic	27811238
PMP1_YEAST	PMP1	physical	26404137

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.