dbPTM

RPB7_YEAST - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RPB7_YEAST
UniProt AC	P34087
Protein Name	DNA-directed RNA polymerase II subunit RPB7
Gene Name	RPB7
Organism	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Sequence Length	171
Subcellular Localization	Nucleus. Cytoplasm. Cytoplasm, P-body. Seems to shuttle between nucleus and cytoplasm in a complex with RPB4.
Protein Description	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. The RPB4-RPB7 subcomplex recruits FCP1 to Pol II..
Protein Sequence	MFFIKDLSLNITLHPSFFGPRMKQYLKTKLLEEVEGSCTGKFGYILCVLDYDNIDIQRGRILPTDGSAEFNVKYRAVVFKPFKGEVVDGTVVSCSQHGFEVQVGPMKVFVTKHLMPQDLTFNAGSNPPSYQSSEDVITIKSRIRVKIEGCISQVSSIHAIGSIKEDYLGAI

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
27	Ubiquitination	PRMKQYLKTKLLEEV HHHHHHHHHHHHHHH	38.38	22817900
29	Ubiquitination	MKQYLKTKLLEEVEG HHHHHHHHHHHHHHC	49.87	23749301
80	Ubiquitination	KYRAVVFKPFKGEVV EEEEEEEECCCCEEE	37.62	23749301
83	Ubiquitination	AVVFKPFKGEVVDGT EEEEECCCCEEECCE	63.79	22817900
112	Acetylation	PMKVFVTKHLMPQDL CEEEEEECCCCCCCC	29.46	24489116
120	Phosphorylation	HLMPQDLTFNAGSNP CCCCCCCEECCCCCC	24.11	19823750
125	Phosphorylation	DLTFNAGSNPPSYQS CCEECCCCCCCCCCC	43.69	19823750
129	Phosphorylation	NAGSNPPSYQSSEDV CCCCCCCCCCCCCCE	36.74	19823750
130	Phosphorylation	AGSNPPSYQSSEDVI CCCCCCCCCCCCCEE	20.42	19823750
132	Phosphorylation	SNPPSYQSSEDVITI CCCCCCCCCCCEEEE	27.71	19823750
133	Phosphorylation	NPPSYQSSEDVITIK CCCCCCCCCCEEEEC	23.45	19823750
138	Phosphorylation	QSSEDVITIKSRIRV CCCCCEEEECEEEEE	23.38	19823750

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	RSP5	P39940	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RPB7_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RPB7_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RPB3_YEAST	RPB3	physical	11805826
RHC31_YEAST	AOS1	physical	11805826
RPB11_YEAST	RPB11	physical	11805826
RPB2_YEAST	RPB2	physical	11805826
RPB4_YEAST	RPB4	physical	11805826
RPAB1_YEAST	RPB5	physical	11805826
RPAB3_YEAST	RPB8	physical	11805826
RPB1_YEAST	RPO21	physical	11805826
SPT4_YEAST	SPT4	physical	11805826
SPT5_YEAST	SPT5	physical	11805826
T2FA_YEAST	TFG1	physical	11805826
T2FB_YEAST	TFG2	physical	11805826
RPB1_YEAST	RPO21	physical	10082533
RPB2_YEAST	RPB2	physical	10082533
RPB4_YEAST	RPB4	physical	14530281
NRD1_YEAST	NRD1	physical	12907709
RPB4_YEAST	RPB4	physical	7579693
RPB4_YEAST	RPB4	physical	15913559
RPB4_YEAST	RPB4	physical	1985924
RHC31_YEAST	AOS1	physical	16429126
RPB11_YEAST	RPB11	physical	16429126
RPB4_YEAST	RPB4	physical	16429126
RPAB1_YEAST	RPB5	physical	16429126
SPT4_YEAST	SPT4	physical	16429126
SPT5_YEAST	SPT5	physical	16429126
T2FA_YEAST	TFG1	physical	16429126
RPB2_YEAST	RPB2	physical	16429126
RPB3_YEAST	RPB3	physical	16429126
RPAB3_YEAST	RPB8	physical	16429126
RPB1_YEAST	RPO21	physical	16429126
T2FB_YEAST	TFG2	physical	16429126
XRN1_YEAST	XRN1	genetic	17875743
RPB4_YEAST	RPB4	genetic	17875743
LSM2_YEAST	LSM2	physical	17875743
PAT1_YEAST	PAT1	physical	17875743
RPB1_YEAST	RPO21	physical	19394294
RPB2_YEAST	RPB2	physical	19394294
RPB4_YEAST	RPB4	physical	19394294
RPAB1_YEAST	RPB5	physical	19394294
RPAB2_YEAST	RPO26	physical	19394294
RPB9_YEAST	RPB9	physical	19394294
RPAB5_YEAST	RPB10	physical	19394294
RPB11_YEAST	RPB11	physical	19394294
RPAB4_YEAST	RPC10	physical	19394294
RTR1_YEAST	RTR1	physical	19394294
RPAB3_YEAST	RPB8	physical	19394294
RPB3_YEAST	RPB3	physical	19394294
RPB4_YEAST	RPB4	physical	17056745
RPAB1_YEAST	RPB5	physical	20720002
RPAB2_YEAST	RPO26	physical	20720002
RPB4_YEAST	RPB4	physical	20720002
CET1_YEAST	CET1	physical	20720002
MCE1_YEAST	CEG1	physical	20720002
RPB3_YEAST	RPB3	physical	20720002
RPB2_YEAST	RPB2	physical	20720002
RPB1_YEAST	RPO21	physical	20720002
EIF3J_YEAST	HCR1	genetic	21074047
RPB4_YEAST	RPB4	physical	16818233
MED2_YEAST	MED2	genetic	17339209
RPAB4_YEAST	RPC10	physical	23418395
RPB9_YEAST	RPB9	physical	23418395
RPB11_YEAST	RPB11	physical	23418395
RPB4_YEAST	RPB4	physical	23418395
RPB3_YEAST	RPB3	physical	23418395
RPAB5_YEAST	RPB10	physical	23418395
RPAB2_YEAST	RPO26	physical	23418395
RPB1_YEAST	RPO21	physical	23418395
RPAB1_YEAST	RPB5	physical	23418395
RPB2_YEAST	RPB2	physical	23418395
RPAB3_YEAST	RPB8	physical	23418395
SPT5_YEAST	SPT5	physical	23418395
SPT4_YEAST	SPT4	physical	23418395
GPN1_YEAST	NPA3	physical	23418395
DBP5_YEAST	DBP5	genetic	22963203
RPB7_YEAST	RPB7	physical	24358479
RPB4_YEAST	RPB4	physical	24358479
RPAB1_YEAST	RPB5	physical	24358479
RPAB2_YEAST	RPO26	physical	24358479
RPAB3_YEAST	RPB8	physical	24358479
RPB3_YEAST	RPB3	physical	24358479
RTR1_YEAST	RTR1	physical	24358479
RPB1_YEAST	RPO21	physical	24358479
DGR2_YEAST	DGR2	physical	24358479
SPT5_YEAST	SPT5	physical	24358479
SWI3_YEAST	SWI3	physical	24358479
RPB2_YEAST	RPB2	physical	24358479
BRE2_YEAST	BRE2	physical	24358479
TFB2_YEAST	TFB2	physical	24358479
KAPB_YEAST	TPK2	physical	24358479
RPH1_YEAST	RPH1	physical	24358479
PAT1_YEAST	PAT1	physical	24358479
EIF3A_YEAST	RPG1	physical	24358479
RPB1_YEAST	RPO21	physical	24513203
RPB2_YEAST	RPB2	physical	24513203
SPT5_YEAST	SPT5	physical	24513203
SWI3_YEAST	SWI3	physical	24513203
DGR2_YEAST	DGR2	physical	24513203
RPH1_YEAST	RPH1	physical	24513203
PAT1_YEAST	PAT1	physical	24513203
EIF3A_YEAST	RPG1	physical	24513203
BRE2_YEAST	BRE2	physical	24513203
TFB2_YEAST	TFB2	physical	24513203
KAPB_YEAST	TPK2	physical	24513203
RPB3_YEAST	RPB3	physical	24513203
RPB4_YEAST	RPB4	physical	24513203
RPAB1_YEAST	RPB5	physical	24513203
RPAB2_YEAST	RPO26	physical	24513203
RPAB3_YEAST	RPB8	physical	24513203
RTR1_YEAST	RTR1	physical	24513203
POP2_YEAST	POP2	physical	25315781
SUB1_YEAST	SUB1	physical	27924005

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RPB7_YEAST

Related Literatures of Post-Translational Modification