| UniProt ID | RPB4_YEAST | |
|---|---|---|
| UniProt AC | P20433 | |
| Protein Name | DNA-directed RNA polymerase II subunit RPB4 | |
| Gene Name | RPB4 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 221 | |
| Subcellular Localization | Nucleus. Cytoplasm. Cytoplasm, P-body. Seems to shuttle between nucleus and cytoplasm in a complex with RPB7. | |
| Protein Description | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. The RPB4-RPB7 subcomplex is necessary for promoter-directed transcription initiation but is not required for recruitment of Pol II to active preinitiation complexes and seems to be dispensable for transcription elongation and termination. The RPB4-RPB7 subcomplex recruits FCP1 to Pol II. Involved in DNA repair of damage in the transcribed strand. RPB4 is dispensable under optimal growth conditions, but becomes essential during heat or cold shock and under nutrient depletion. Suppresses the RBP9-mediated transcription-coupled repair (TCR) subpathway of nucleotide excision repair (NER) but facilitates the RAD26-mediated TCR subpathway. Under stress conditions only, involved in mRNA export to the cytoplasm. Involved in mRNA decay. Promotes or enhances the deadenylation process of specific mRNAs and may recruit PAT1 and the LSM1-7 complex to these mRNAs, thus stimulating their decapping and further decay.. | |
| Protein Sequence | MNVSTSTFQTRRRRLKKVEEEENAATLQLGQEFQLKQINHQGEEEELIALNLSEARLVIKEALVERRRAFKRSQKKHKKKHLKHENANDETTAVEDEDDDLDEDDVNADDDDFMHSETREKELESIDVLLEQTTGGNNKDLKNTMQYLTNFSRFRDQETVGAVIQLLKSTGLHPFEVAQLGSLACDTADEAKTLIPSLNNKISDDELERILKELSNLETLY | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 1 | Acetylation | -------MNVSTSTF -------CCCCHHHH | 11.45 | 22814378 | |
| 91 | Phosphorylation | HENANDETTAVEDED CCCCCCCCCCCCCCC | 23.95 | 28889911 | |
| 92 | Phosphorylation | ENANDETTAVEDEDD CCCCCCCCCCCCCCC | 27.02 | 28889911 | |
| 125 | Phosphorylation | TREKELESIDVLLEQ HHHHHHHHHHHHHHH | 36.39 | 22369663 | |
| 133 | Phosphorylation | IDVLLEQTTGGNNKD HHHHHHHCCCCCCHH | 20.31 | 22369663 | |
| 134 | Phosphorylation | DVLLEQTTGGNNKDL HHHHHHCCCCCCHHH | 42.67 | 25521595 | |
| 142 | Ubiquitination | GGNNKDLKNTMQYLT CCCCHHHHHHHHHHH | 61.58 | 17644757 | |
| 144 | Phosphorylation | NNKDLKNTMQYLTNF CCHHHHHHHHHHHHH | 12.22 | 27017623 | |
| 147 | Phosphorylation | DLKNTMQYLTNFSRF HHHHHHHHHHHHHHH | 12.50 | 27017623 | |
| 149 | Phosphorylation | KNTMQYLTNFSRFRD HHHHHHHHHHHHHCC | 29.32 | 27017623 | |
| 152 | Phosphorylation | MQYLTNFSRFRDQET HHHHHHHHHHCCHHH | 32.36 | 27017623 | |
| 168 | Ubiquitination | GAVIQLLKSTGLHPF HHHHHHHHHCCCCHH | 55.34 | 17644757 | |
| 193 | Phosphorylation | DTADEAKTLIPSLNN CCHHHHHHHHHHHCC | 36.65 | 19823750 | |
| 197 | Phosphorylation | EAKTLIPSLNNKISD HHHHHHHHHCCCCCH | 36.41 | 19795423 | |
| 212 | Ubiquitination | DELERILKELSNLET HHHHHHHHHHHCCCC | 54.99 | 23749301 | |
| 215 | Phosphorylation | ERILKELSNLETLY- HHHHHHHHCCCCCC- | 40.22 | 30377154 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of RPB4_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of RPB4_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of RPB4_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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