| UniProt ID | NOT4_YEAST | |
|---|---|---|
| UniProt AC | P34909 | |
| Protein Name | General negative regulator of transcription subunit 4 | |
| Gene Name | MOT2 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 587 | |
| Subcellular Localization | Cytoplasm . Nucleus. | |
| Protein Description | Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.. | |
| Protein Sequence | MMNPHVQENLQAIHNALSNFDTSFLSEDEEDYCPLCIEPMDITDKNFFPCPCGYQICQFCYNNIRQNPELNGRCPACRRKYDDENVRYVTLSPEELKMERAKLARKEKERKHREKERKENEYTNRKHLSGTRVIQKNLVYVVGINPPVPYEEVAPTLKSEKYFGQYGKINKIVVNRKTPHSNNTTSEHYHHHSPGYGVYITFGSKDDAARCIAQVDGTYMDGRLIKAAYGTTKYCSSYLRGLPCPNPNCMFLHEPGEEADSFNKRELHNKQQAQQQSGGTAFTRSGIHNNISTSTAGSNTNLLSENFTGTPSPAAMRAQLHHDSHTNAGTPVLTPAPVPAGSNPWGVTQSATPVTSINLSKNSSSINLPTLNDSLGHHTTPTTENTITSTTTTTNTNATSHSHGSKKKQSLAAEEYKDPYDALGNAVDFLDARLHSLSNYQKRPISIKSNIIDEETYKKYPSLFSWDKIEASKKSDNTLANKLVEILAIKPIDYTASVVQFLQSVNVGVNDNITITDNTKTPTQPIRLQTVSQQIQPPLNVSTPPPGIFGPQHKVPIQQQQMGDTSSRNSSDLLNQLINGRKIIAGN | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 45 | Ubiquitination | EPMDITDKNFFPCPC CCCCCCCCCCCCCCC | 47.18 | 17644757 | |
| 88 | Phosphorylation | YDDENVRYVTLSPEE CCCCCCEEEECCHHH | 8.10 | 28889911 | |
| 90 | Phosphorylation | DENVRYVTLSPEELK CCCCEEEECCHHHHH | 16.75 | 25521595 | |
| 92 | Phosphorylation | NVRYVTLSPEELKME CCEEEECCHHHHHHH | 22.39 | 22369663 | |
| 97 | Ubiquitination | TLSPEELKMERAKLA ECCHHHHHHHHHHHH | 41.83 | 23749301 | |
| 97 | Acetylation | TLSPEELKMERAKLA ECCHHHHHHHHHHHH | 41.83 | 24489116 | |
| 129 | Phosphorylation | YTNRKHLSGTRVIQK HCCCHHCCCCEEEEC | 37.53 | 21440633 | |
| 168 | Ubiquitination | KYFGQYGKINKIVVN CHHCCCCCEEEEEEC | 37.87 | 23749301 | |
| 171 | Ubiquitination | GQYGKINKIVVNRKT CCCCCEEEEEECCCC | 40.11 | 23749301 | |
| 177 | Ubiquitination | NKIVVNRKTPHSNNT EEEEECCCCCCCCCC | 62.29 | 22817900 | |
| 205 | Ubiquitination | VYITFGSKDDAARCI EEEEECCHHHHHHHE | 61.07 | 23749301 | |
| 226 | Ubiquitination | YMDGRLIKAAYGTTK EECCEEHHHHHCCHH | 31.40 | 23749301 | |
| 264 | Ubiquitination | EEADSFNKRELHNKQ CCCCCCCHHHHHHHH | 45.28 | 23749301 | |
| 270 | Ubiquitination | NKRELHNKQQAQQQS CHHHHHHHHHHHHHH | 32.80 | 23749301 | |
| 277 | Phosphorylation | KQQAQQQSGGTAFTR HHHHHHHHCCCCCCC | 34.55 | 28889911 | |
| 285 | Phosphorylation | GGTAFTRSGIHNNIS CCCCCCCCCCCCCCC | 38.76 | 22369663 | |
| 292 | Phosphorylation | SGIHNNISTSTAGSN CCCCCCCCCCCCCCC | 20.59 | 22369663 | |
| 293 | Phosphorylation | GIHNNISTSTAGSNT CCCCCCCCCCCCCCC | 26.71 | 22369663 | |
| 294 | Phosphorylation | IHNNISTSTAGSNTN CCCCCCCCCCCCCCC | 14.96 | 22369663 | |
| 295 | Phosphorylation | HNNISTSTAGSNTNL CCCCCCCCCCCCCCC | 34.82 | 22369663 | |
| 298 | Phosphorylation | ISTSTAGSNTNLLSE CCCCCCCCCCCCCCC | 37.71 | 22369663 | |
| 300 | Phosphorylation | TSTAGSNTNLLSENF CCCCCCCCCCCCCCC | 29.01 | 22369663 | |
| 304 | Phosphorylation | GSNTNLLSENFTGTP CCCCCCCCCCCCCCC | 33.39 | 22369663 | |
| 308 | Phosphorylation | NLLSENFTGTPSPAA CCCCCCCCCCCCHHH | 52.29 | 22369663 | |
| 310 | Phosphorylation | LSENFTGTPSPAAMR CCCCCCCCCCHHHHH | 19.96 | 22369663 | |
| 312 | Phosphorylation | ENFTGTPSPAAMRAQ CCCCCCCCHHHHHHH | 27.32 | 22369663 | |
| 324 | Phosphorylation | RAQLHHDSHTNAGTP HHHHCCCCCCCCCCC | 28.05 | 28889911 | |
| 326 | Phosphorylation | QLHHDSHTNAGTPVL HHCCCCCCCCCCCCC | 30.62 | 21440633 | |
| 330 | Phosphorylation | DSHTNAGTPVLTPAP CCCCCCCCCCCCCCC | 14.07 | 19779198 | |
| 334 | Phosphorylation | NAGTPVLTPAPVPAG CCCCCCCCCCCCCCC | 20.03 | 20386698 | |
| 342 | Phosphorylation | PAPVPAGSNPWGVTQ CCCCCCCCCCCCCCC | 41.65 | 20386698 | |
| 348 | Phosphorylation | GSNPWGVTQSATPVT CCCCCCCCCCCCCCC | 16.93 | 19779198 | |
| 350 | Phosphorylation | NPWGVTQSATPVTSI CCCCCCCCCCCCCEE | 25.41 | 21440633 | |
| 352 | Phosphorylation | WGVTQSATPVTSINL CCCCCCCCCCCEEEC | 24.44 | 28889911 | |
| 360 | Phosphorylation | PVTSINLSKNSSSIN CCCEEECCCCCCCCC | 25.88 | 23749301 | |
| 363 | Phosphorylation | SINLSKNSSSINLPT EEECCCCCCCCCCCC | 29.24 | 21551504 | |
| 364 | Phosphorylation | INLSKNSSSINLPTL EECCCCCCCCCCCCC | 44.63 | 21551504 | |
| 365 | Phosphorylation | NLSKNSSSINLPTLN ECCCCCCCCCCCCCC | 18.47 | 23749301 | |
| 370 | Phosphorylation | SSSINLPTLNDSLGH CCCCCCCCCCCCCCC | 41.16 | 28889911 | |
| 374 | Phosphorylation | NLPTLNDSLGHHTTP CCCCCCCCCCCCCCC | 34.87 | 30377154 | |
| 379 | Phosphorylation | NDSLGHHTTPTTENT CCCCCCCCCCCCCCE | 28.88 | 21440633 | |
| 380 | Phosphorylation | DSLGHHTTPTTENTI CCCCCCCCCCCCCEE | 17.80 | 23749301 | |
| 382 | Phosphorylation | LGHHTTPTTENTITS CCCCCCCCCCCEEEE | 44.65 | 30377154 | |
| 383 | Phosphorylation | GHHTTPTTENTITST CCCCCCCCCCEEEEE | 28.57 | 21551504 | |
| 408 | Ubiquitination | HSHGSKKKQSLAAEE CCCCCHHHHCCCHHH | 49.18 | 17644757 | |
| 417 | Ubiquitination | SLAAEEYKDPYDALG CCCHHHHCCHHHHHH | 56.35 | 22106047 | |
| 436 | Phosphorylation | FLDARLHSLSNYQKR HHHHHHHHCCCCCCC | 37.78 | 21440633 | |
| 521 | Phosphorylation | TITDNTKTPTQPIRL EECCCCCCCCCCEEE | 29.54 | 20377248 | |
| 523 | Phosphorylation | TDNTKTPTQPIRLQT CCCCCCCCCCEEEEE | 52.81 | 19823750 | |
| 530 | Phosphorylation | TQPIRLQTVSQQIQP CCCEEEEECCCCCCC | 27.07 | 20377248 | |
| 532 | Phosphorylation | PIRLQTVSQQIQPPL CEEEEECCCCCCCCC | 21.54 | 22890988 | |
| 542 | Phosphorylation | IQPPLNVSTPPPGIF CCCCCCCCCCCCCCC | 33.84 | 22369663 | |
| 543 | Phosphorylation | QPPLNVSTPPPGIFG CCCCCCCCCCCCCCC | 35.22 | 22369663 | |
| 565 | Phosphorylation | QQQQMGDTSSRNSSD CCCCCCCCCCCCHHH | 23.91 | 23749301 | |
| 566 | Phosphorylation | QQQMGDTSSRNSSDL CCCCCCCCCCCHHHH | 31.85 | 20377248 | |
| 567 | Phosphorylation | QQMGDTSSRNSSDLL CCCCCCCCCCHHHHH | 37.26 | 21440633 | |
| 570 | Phosphorylation | GDTSSRNSSDLLNQL CCCCCCCHHHHHHHH | 24.90 | 22369663 | |
| 571 | Phosphorylation | DTSSRNSSDLLNQLI CCCCCCHHHHHHHHH | 35.51 | 22369663 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of NOT4_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of NOT4_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of NOT4_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92; THR-300; SER-312;THR-352; SER-542 AND THR-543, AND MASS SPECTROMETRY. | |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-298, AND MASSSPECTROMETRY. | |
| "Large-scale phosphorylation analysis of alpha-factor-arrestedSaccharomyces cerevisiae."; Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,Elias J.E., Gygi S.P.; J. Proteome Res. 6:1190-1197(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-310 AND SER-312, ANDMASS SPECTROMETRY. | |
