| UniProt ID | UBP4_YEAST | |
|---|---|---|
| UniProt AC | P32571 | |
| Protein Name | Ubiquitin carboxyl-terminal hydrolase 4 | |
| Gene Name | DOA4 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 926 | |
| Subcellular Localization |
Cytoplasm. Late endosome membrane Peripheral membrane protein. Recruited to the late endosome by BRO1. |
|
| Protein Description | Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Involved in the ammonium-induced down-regulation of the GAP1 permease and the UME3 destruction in response to oxidative stress. Has a role in the RAD9 checkpoint response to TOP1 poisons. Required for promoting coordination of DNA replication and avoids DNA overreplication.. | |
| Protein Sequence | MEQNIISTIRDECIRHRSKYLTIAQLTAIAEAKINEFIITGKAKDQDLSSLLDKCIDILSIYKKNSKDIKNIISCKNKGAMISSNSVMIIQLNYVYYKVIHIIVTTNIPHLSEFAKIKLHKSTSDEGNGNNNNNEFQLMNIYNTLLETLLKDENIAKIKSFIKSSIKQTKLNHEQEECNLMRTGSYITSNQLNSLISSSANSASSQMEILLIDIRSRLEFNKSHIDTKNIICLEPISFKMSYSDHDLEKKSLITSPNSEIKMFQSRNLFKFIILYTDANEYNVKQQSVLLDILVNHSFEKPISDDFTKIFILESGFPGWLKSNYGRQVSSSFPSNNNIKDDSVYINGNTSGLSLQHLPKMSPSIRHSMDDSMKEMLVAPTPLNHLQQQQQQQSDNDHVLKRSSSFKKLFSNYTSPNPKNSNSNLYSISSLSISSSPSPLPLHSPDPVKGNSLPINYPETPHLWKNSETDFMTNQREQLNHNSFAHIAPINTKAITSPSRTATPKLQRFPQTISMNLNMNSNGHSSATSTIQPSCLSLSNNDSLDHTDVTPTSSHNYDLDFAVGLENLGNSCYMNCIIQCILGTHELTQIFLDDSYAKHININSKLGSKGILAKYFARLVHMMYKEQVDGSKKISISPIKFKLACGSVNSLFKTASQQDCQEFCQFLLDGLHEDLNQCGSNPPLKELSQEAEARREKLSLRIASSIEWERFLTTDFSVIVDLFQGQYASRLKCKVCSHTSTTYQPFTVLSIPIPKKNSRNNITIEDCFREFTKCENLEVDEQWLCPHCEKRQPSTKQLTITRLPRNLIVHLKRFDNLLNKNNDFVIYPFLLDLTPFWANDFDGVFPPGVNDDELPIRGQIPPFKYELYGVACHFGTLYGGHYTAYVKKGLKKGWLYFDDTKYKPVKNKADAINSNAYVLFYHRVYGV | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 44 | Acetylation | FIITGKAKDQDLSSL EEEECCCCCCCHHHH | 60.45 | 24489116 | |
| 251 | Phosphorylation | DHDLEKKSLITSPNS CCCCHHCCCCCCCCH | 35.75 | 19823750 | |
| 254 | Phosphorylation | LEKKSLITSPNSEIK CHHCCCCCCCCHHHH | 43.34 | 19823750 | |
| 255 | Phosphorylation | EKKSLITSPNSEIKM HHCCCCCCCCHHHHH | 18.32 | 19823750 | |
| 258 | Phosphorylation | SLITSPNSEIKMFQS CCCCCCCHHHHHHHH | 44.05 | 19823750 | |
| 265 | Phosphorylation | SEIKMFQSRNLFKFI HHHHHHHHCCCEEEE | 16.33 | 19823750 | |
| 275 | Phosphorylation | LFKFIILYTDANEYN CEEEEEEECCCCCCC | 7.68 | 19823750 | |
| 276 | Phosphorylation | FKFIILYTDANEYNV EEEEEEECCCCCCCC | 25.78 | 19823750 | |
| 281 | Phosphorylation | LYTDANEYNVKQQSV EECCCCCCCCCHHHH | 25.68 | 19823750 | |
| 334 | Phosphorylation | QVSSSFPSNNNIKDD CCCCCCCCCCCCCCC | 50.62 | 21440633 | |
| 342 | Phosphorylation | NNNIKDDSVYINGNT CCCCCCCEEEECCCC | 27.27 | 22369663 | |
| 344 | Phosphorylation | NIKDDSVYINGNTSG CCCCCEEEECCCCCC | 7.82 | 22369663 | |
| 349 | Phosphorylation | SVYINGNTSGLSLQH EEEECCCCCCCCHHH | 26.10 | 22369663 | |
| 350 | Phosphorylation | VYINGNTSGLSLQHL EEECCCCCCCCHHHC | 41.56 | 22369663 | |
| 353 | Phosphorylation | NGNTSGLSLQHLPKM CCCCCCCCHHHCCCC | 29.52 | 22369663 | |
| 361 | Phosphorylation | LQHLPKMSPSIRHSM HHHCCCCCHHHHHCC | 22.94 | 21440633 | |
| 363 | Phosphorylation | HLPKMSPSIRHSMDD HCCCCCHHHHHCCCH | 25.58 | 21440633 | |
| 367 | Phosphorylation | MSPSIRHSMDDSMKE CCHHHHHCCCHHHHH | 17.66 | 21440633 | |
| 404 | Phosphorylation | HVLKRSSSFKKLFSN HHHHHHHHHHHHHCC | 42.19 | 28889911 | |
| 412 | Phosphorylation | FKKLFSNYTSPNPKN HHHHHCCCCCCCCCC | 13.85 | 29136822 | |
| 413 | Phosphorylation | KKLFSNYTSPNPKNS HHHHCCCCCCCCCCC | 41.79 | 29136822 | |
| 414 | Phosphorylation | KLFSNYTSPNPKNSN HHHCCCCCCCCCCCC | 16.60 | 29136822 | |
| 443 | Phosphorylation | PSPLPLHSPDPVKGN CCCCCCCCCCCCCCC | 38.39 | 17376168 | |
| 451 | Phosphorylation | PDPVKGNSLPINYPE CCCCCCCCCCCCCCC | 44.16 | 21440633 | |
| 459 | Phosphorylation | LPINYPETPHLWKNS CCCCCCCCCCCCCCC | 16.11 | 21440633 | |
| 495 | Phosphorylation | PINTKAITSPSRTAT CCCCCCCCCCCCCCC | 39.17 | 22369663 | |
| 496 | Phosphorylation | INTKAITSPSRTATP CCCCCCCCCCCCCCH | 18.11 | 22369663 | |
| 498 | Phosphorylation | TKAITSPSRTATPKL CCCCCCCCCCCCHHH | 41.87 | 22369663 | |
| 500 | Phosphorylation | AITSPSRTATPKLQR CCCCCCCCCCHHHCC | 38.94 | 22369663 | |
| 502 | Phosphorylation | TSPSRTATPKLQRFP CCCCCCCCHHHCCCC | 21.97 | 20377248 | |
| 613 | Acetylation | GSKGILAKYFARLVH CCHHHHHHHHHHHHH | 37.14 | 24489116 | |
| 636 | Phosphorylation | GSKKISISPIKFKLA CCCEEEECCCCHHHH | 17.49 | 28889911 | |
| 646 | Phosphorylation | KFKLACGSVNSLFKT CHHHHHCCHHHHHHC | 20.29 | 21126336 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of UBP4_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of UBP4_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of UBP4_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-414 AND SER-443, ANDMASS SPECTROMETRY. | |
