| UniProt ID | NOT3_YEAST | |
|---|---|---|
| UniProt AC | P06102 | |
| Protein Name | General negative regulator of transcription subunit 3 | |
| Gene Name | NOT3 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 836 | |
| Subcellular Localization | Cytoplasm . Nucleus. | |
| Protein Description | Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.. | |
| Protein Sequence | MAHRKLQQEVDRVFKKINEGLEIFNSYYERHESCTNNPSQKDKLESDLKREVKKLQRLREQIKSWQSSPDIKDKDSLLDYRRSVEIAMEKYKAVEKASKEKAYSNISLKKSETLDPQERERRDISEYLSQMIDELERQYDSLQVEIDKLLLLNKKKKTSSTTNDEKKEQYKRFQARYRWHQQQMELALRLLANEELDPQDVKNVQDDINYFVESNQDPDFVEDETIYDGLNLQSNEAIAHEVAQYFASQNAEDNNTSDANESLQDISKLSKKEQRKLEREAKKAAKLAAKNATGAAIPVAGPSSTPSPVIPVADASKETERSPSSSPIHNATKPEEAVKTSIKSPRSSADNLLPSLQKSPSSATPETPTNVHTHIHQTPNGITGATTLKPATLPAKPAGELKWAVAASQAVEKDRKVTSASSTISNTSTKTPTTAAATTTSSNANSRIGSALNTPKLSTSSLSLQPDNTGASSSAATAAAVLAAGAAAVHQNNQAFYRNMSSSHHPLVSLATNPKSEHEVATTVNQNGPENTTKKVMEQKEEESPEERNKLQVPTFGVFDDDFESDRDSETEPEEEEQPSTPKYLSLEQREAKTNEIKKEFVSDFETLLLPSGVQEFIMSSELYNSQIESKITYKRSRDMCEISRLVEVPQGVNPPSPLDAFRSTQQWDVMRCSLRDIIIGSERLKEDSSSIYAKILENFRTLEMFSLFYNYYFAITPLEREIAYKILNERDWKVSKDGTMWFLRQGEVKFFNEICEVGDYKIFKLDDWTVIDKINFRLDYSFLQPPVDTASEVRDVSVDNNNVNDQSNVTLEQQKQEISHGKQLLKQLKQGKISV | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 72 | Acetylation | WQSSPDIKDKDSLLD HHCCCCCCCCHHHHH | 67.13 | 24489116 | |
| 83 | Phosphorylation | SLLDYRRSVEIAMEK HHHHHHHHHHHHHHH | 18.33 | 27017623 | |
| 90 | Acetylation | SVEIAMEKYKAVEKA HHHHHHHHHHHHHHH | 38.68 | 24489116 | |
| 107 | Phosphorylation | EKAYSNISLKKSETL HHHHHCCCCCCCCCC | 38.08 | 15665377 | |
| 110 | Ubiquitination | YSNISLKKSETLDPQ HHCCCCCCCCCCCHH | 59.84 | 23749301 | |
| 111 | Phosphorylation | SNISLKKSETLDPQE HCCCCCCCCCCCHHH | 33.94 | 28889911 | |
| 113 | Phosphorylation | ISLKKSETLDPQERE CCCCCCCCCCHHHHH | 43.48 | 23749301 | |
| 257 | Phosphorylation | NAEDNNTSDANESLQ CCCCCCCCCHHHHHH | 36.53 | 28889911 | |
| 290 | Ubiquitination | KAAKLAAKNATGAAI HHHHHHHHHCCCCCC | 41.32 | 23749301 | |
| 293 | Phosphorylation | KLAAKNATGAAIPVA HHHHHHCCCCCCCCC | 36.43 | 22369663 | |
| 303 | Phosphorylation | AIPVAGPSSTPSPVI CCCCCCCCCCCCCEE | 46.02 | 22369663 | |
| 304 | Phosphorylation | IPVAGPSSTPSPVIP CCCCCCCCCCCCEEE | 47.69 | 22369663 | |
| 305 | Phosphorylation | PVAGPSSTPSPVIPV CCCCCCCCCCCEEEC | 31.93 | 22369663 | |
| 307 | Phosphorylation | AGPSSTPSPVIPVAD CCCCCCCCCEEECCC | 31.71 | 22369663 | |
| 316 | Phosphorylation | VIPVADASKETERSP EEECCCCCCCCCCCC | 31.41 | 22369663 | |
| 317 | Ubiquitination | IPVADASKETERSPS EECCCCCCCCCCCCC | 71.08 | 23749301 | |
| 319 | Phosphorylation | VADASKETERSPSSS CCCCCCCCCCCCCCC | 40.20 | 22369663 | |
| 322 | Phosphorylation | ASKETERSPSSSPIH CCCCCCCCCCCCCCC | 23.93 | 22369663 | |
| 324 | Phosphorylation | KETERSPSSSPIHNA CCCCCCCCCCCCCCC | 44.70 | 22369663 | |
| 325 | Phosphorylation | ETERSPSSSPIHNAT CCCCCCCCCCCCCCC | 43.44 | 22369663 | |
| 326 | Phosphorylation | TERSPSSSPIHNATK CCCCCCCCCCCCCCC | 32.01 | 22369663 | |
| 332 | Phosphorylation | SSPIHNATKPEEAVK CCCCCCCCCHHHHHH | 53.07 | 20377248 | |
| 340 | Phosphorylation | KPEEAVKTSIKSPRS CHHHHHHHHCCCCCC | 29.14 | 22369663 | |
| 341 | Phosphorylation | PEEAVKTSIKSPRSS HHHHHHHHCCCCCCC | 23.46 | 21440633 | |
| 343 | Ubiquitination | EAVKTSIKSPRSSAD HHHHHHCCCCCCCHH | 54.92 | 23749301 | |
| 344 | Phosphorylation | AVKTSIKSPRSSADN HHHHHCCCCCCCHHH | 24.71 | 22369663 | |
| 347 | Phosphorylation | TSIKSPRSSADNLLP HHCCCCCCCHHHCCH | 33.01 | 22369663 | |
| 348 | Phosphorylation | SIKSPRSSADNLLPS HCCCCCCCHHHCCHH | 40.70 | 22369663 | |
| 355 | Phosphorylation | SADNLLPSLQKSPSS CHHHCCHHHCCCCCC | 43.32 | 22369663 | |
| 359 | Phosphorylation | LLPSLQKSPSSATPE CCHHHCCCCCCCCCC | 19.63 | 20377248 | |
| 361 | Phosphorylation | PSLQKSPSSATPETP HHHCCCCCCCCCCCC | 39.43 | 20377248 | |
| 362 | Phosphorylation | SLQKSPSSATPETPT HHCCCCCCCCCCCCC | 39.28 | 20377248 | |
| 364 | Phosphorylation | QKSPSSATPETPTNV CCCCCCCCCCCCCCE | 24.49 | 20377248 | |
| 367 | Phosphorylation | PSSATPETPTNVHTH CCCCCCCCCCCEEEC | 36.29 | 21440633 | |
| 369 | Phosphorylation | SATPETPTNVHTHIH CCCCCCCCCEEECEE | 58.24 | 20377248 | |
| 373 | Phosphorylation | ETPTNVHTHIHQTPN CCCCCEEECEEECCC | 20.45 | 20377248 | |
| 378 | Phosphorylation | VHTHIHQTPNGITGA EEECEEECCCCCCCC | 12.45 | 20377248 | |
| 383 | Phosphorylation | HQTPNGITGATTLKP EECCCCCCCCCCCCC | 23.35 | 20377248 | |
| 386 | Phosphorylation | PNGITGATTLKPATL CCCCCCCCCCCCCCC | 33.72 | 19779198 | |
| 387 | Phosphorylation | NGITGATTLKPATLP CCCCCCCCCCCCCCC | 32.05 | 19779198 | |
| 392 | Phosphorylation | ATTLKPATLPAKPAG CCCCCCCCCCCCCCC | 41.63 | 19823750 | |
| 413 | Ubiquitination | AASQAVEKDRKVTSA HHHHHHHHHHCCCCC | 57.12 | 23749301 | |
| 416 | Ubiquitination | QAVEKDRKVTSASST HHHHHHHCCCCCCCC | 61.06 | 23749301 | |
| 418 | Phosphorylation | VEKDRKVTSASSTIS HHHHHCCCCCCCCCC | 22.93 | 21440633 | |
| 419 | Phosphorylation | EKDRKVTSASSTISN HHHHCCCCCCCCCCC | 29.19 | 22369663 | |
| 421 | Phosphorylation | DRKVTSASSTISNTS HHCCCCCCCCCCCCC | 27.99 | 23749301 | |
| 422 | Phosphorylation | RKVTSASSTISNTST HCCCCCCCCCCCCCC | 29.49 | 22369663 | |
| 423 | Phosphorylation | KVTSASSTISNTSTK CCCCCCCCCCCCCCC | 26.40 | 22369663 | |
| 425 | Phosphorylation | TSASSTISNTSTKTP CCCCCCCCCCCCCCC | 33.86 | 22369663 | |
| 427 | Phosphorylation | ASSTISNTSTKTPTT CCCCCCCCCCCCCCC | 30.86 | 22369663 | |
| 428 | Phosphorylation | SSTISNTSTKTPTTA CCCCCCCCCCCCCCE | 31.96 | 22369663 | |
| 429 | Phosphorylation | STISNTSTKTPTTAA CCCCCCCCCCCCCEE | 36.58 | 23749301 | |
| 430 | Ubiquitination | TISNTSTKTPTTAAA CCCCCCCCCCCCEEE | 52.88 | 23749301 | |
| 431 | Phosphorylation | ISNTSTKTPTTAAAT CCCCCCCCCCCEEEE | 26.42 | 22890988 | |
| 433 | Phosphorylation | NTSTKTPTTAAATTT CCCCCCCCCEEEEEC | 34.01 | 22890988 | |
| 434 | Phosphorylation | TSTKTPTTAAATTTS CCCCCCCCEEEEECC | 18.59 | 22890988 | |
| 438 | Phosphorylation | TPTTAAATTTSSNAN CCCCEEEEECCCCHH | 26.36 | 22890988 | |
| 439 | Phosphorylation | PTTAAATTTSSNANS CCCEEEEECCCCHHH | 21.69 | 22890988 | |
| 440 | Phosphorylation | TTAAATTTSSNANSR CCEEEEECCCCHHHH | 26.29 | 22369663 | |
| 441 | Phosphorylation | TAAATTTSSNANSRI CEEEEECCCCHHHHC | 21.36 | 22369663 | |
| 442 | Phosphorylation | AAATTTSSNANSRIG EEEEECCCCHHHHCH | 37.56 | 22369663 | |
| 446 | Phosphorylation | TTSSNANSRIGSALN ECCCCHHHHCHHCCC | 23.90 | 22369663 | |
| 450 | Phosphorylation | NANSRIGSALNTPKL CHHHHCHHCCCCCCC | 27.49 | 22369663 | |
| 454 | Phosphorylation | RIGSALNTPKLSTSS HCHHCCCCCCCCCCC | 23.73 | 22369663 | |
| 458 | Phosphorylation | ALNTPKLSTSSLSLQ CCCCCCCCCCCCCCC | 32.16 | 22369663 | |
| 459 | Phosphorylation | LNTPKLSTSSLSLQP CCCCCCCCCCCCCCC | 32.54 | 22369663 | |
| 460 | Phosphorylation | NTPKLSTSSLSLQPD CCCCCCCCCCCCCCC | 26.38 | 22369663 | |
| 461 | Phosphorylation | TPKLSTSSLSLQPDN CCCCCCCCCCCCCCC | 23.28 | 22369663 | |
| 463 | Phosphorylation | KLSTSSLSLQPDNTG CCCCCCCCCCCCCCC | 27.23 | 22369663 | |
| 469 | Phosphorylation | LSLQPDNTGASSSAA CCCCCCCCCCCHHHH | 41.32 | 22369663 | |
| 472 | Phosphorylation | QPDNTGASSSAATAA CCCCCCCCHHHHHHH | 26.60 | 22369663 | |
| 473 | Phosphorylation | PDNTGASSSAATAAA CCCCCCCHHHHHHHH | 24.38 | 22369663 | |
| 474 | Phosphorylation | DNTGASSSAATAAAV CCCCCCHHHHHHHHH | 21.30 | 22369663 | |
| 477 | Phosphorylation | GASSSAATAAAVLAA CCCHHHHHHHHHHHH | 19.29 | 22369663 | |
| 497 | Phosphorylation | HQNNQAFYRNMSSSH HHCCHHHHHCCCCCC | 12.23 | 22369663 | |
| 501 | Phosphorylation | QAFYRNMSSSHHPLV HHHHHCCCCCCCCCE | 32.03 | 28889911 | |
| 502 | Phosphorylation | AFYRNMSSSHHPLVS HHHHCCCCCCCCCEE | 23.95 | 21440633 | |
| 503 | Phosphorylation | FYRNMSSSHHPLVSL HHHCCCCCCCCCEEC | 20.64 | 19779198 | |
| 516 | Phosphorylation | SLATNPKSEHEVATT ECCCCCCCHHCHHCC | 45.50 | 28889911 | |
| 534 | Ubiquitination | NGPENTTKKVMEQKE CCCCHHHHHHHHHHH | 41.13 | 23749301 | |
| 535 | Ubiquitination | GPENTTKKVMEQKEE CCCHHHHHHHHHHHH | 44.73 | 22817900 | |
| 540 | Ubiquitination | TKKVMEQKEEESPEE HHHHHHHHHHCCHHH | 54.51 | 23749301 | |
| 544 | Phosphorylation | MEQKEEESPEERNKL HHHHHHCCHHHHHCC | 40.67 | 23749301 | |
| 555 | Phosphorylation | RNKLQVPTFGVFDDD HHCCCCCCCCCCCCC | 33.88 | 21440633 | |
| 565 | Phosphorylation | VFDDDFESDRDSETE CCCCCCCCCCCCCCC | 37.63 | 19823750 | |
| 569 | Phosphorylation | DFESDRDSETEPEEE CCCCCCCCCCCCHHH | 47.51 | 19823750 | |
| 571 | Phosphorylation | ESDRDSETEPEEEEQ CCCCCCCCCCHHHCC | 61.87 | 20377248 | |
| 580 | Phosphorylation | PEEEEQPSTPKYLSL CHHHCCCCCCCCCCH | 57.97 | 20377248 | |
| 581 | Phosphorylation | EEEEQPSTPKYLSLE HHHCCCCCCCCCCHH | 30.40 | 23749301 | |
| 657 | Phosphorylation | PQGVNPPSPLDAFRS CCCCCCCCHHHHHHC | 39.07 | 17330950 | |
| 686 | Ubiquitination | IIGSERLKEDSSSIY HHCCHHHCCCCCHHH | 66.74 | 23749301 | |
| 737 | Acetylation | ERDWKVSKDGTMWFL HCCCEECCCCCEEEE | 64.26 | 24489116 | |
| 798 | Phosphorylation | ASEVRDVSVDNNNVN HHHEEEEECCCCCCC | 28.21 | 25752575 | |
| 808 | Phosphorylation | NNNVNDQSNVTLEQQ CCCCCCCCCCCHHHH | 35.81 | 28889911 | |
| 816 | Ubiquitination | NVTLEQQKQEISHGK CCCHHHHHHHHHHHH | 49.98 | 23749301 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of NOT3_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of NOT3_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of NOT3_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "A multidimensional chromatography technology for in-depthphosphoproteome analysis."; Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; Mol. Cell. Proteomics 7:1389-1396(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-257; SER-344; SER-347;SER-348; SER-442; SER-450; THR-454; SER-460; SER-565 AND SER-657, ANDMASS SPECTROMETRY. | |
| "Proteome-wide identification of in vivo targets of DNA damagecheckpoint kinases."; Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-322; SER-324; SER-326;SER-344; SER-347 AND SER-348, AND MASS SPECTROMETRY. | |
| "Analysis of phosphorylation sites on proteins from Saccharomycescerevisiae by electron transfer dissociation (ETD) massspectrometry."; Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.; Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-322; SER-326; SER-344AND THR-373, AND MASS SPECTROMETRY. | |
| "Large-scale phosphorylation analysis of alpha-factor-arrestedSaccharomyces cerevisiae."; Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,Elias J.E., Gygi S.P.; J. Proteome Res. 6:1190-1197(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-450; THR-454 ANDSER-657, AND MASS SPECTROMETRY. | |
| "Phosphoproteome analysis by mass spectrometry and its application toSaccharomyces cerevisiae."; Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M.,Shabanowitz J., Hunt D.F., White F.M.; Nat. Biotechnol. 20:301-305(2002). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442; SER-446; SER-450AND THR-454, AND MASS SPECTROMETRY. | |
| "Quantitative phosphoproteomics applied to the yeast pheromonesignaling pathway."; Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J.,Mann M., Jensen O.N.; Mol. Cell. Proteomics 4:310-327(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-454, AND MASSSPECTROMETRY. | |
