| UniProt ID | GCN5_YEAST | |
|---|---|---|
| UniProt AC | Q03330 | |
| Protein Name | Histone acetyltransferase GCN5 | |
| Gene Name | GCN5 | |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). | |
| Sequence Length | 439 | |
| Subcellular Localization | Nucleus. | |
| Protein Description | Acetylates histone H2B to form H2BK11ac and H2BK16ac, histone H3 to form H3K9ac, H3K14ac, H3K18ac, H3K23ac, H3K27ac and H3K36ac, with a lower preference histone H4 to form H4K8ac and H4K16ac, and contributes to H2A.Z acetylation. Acetylation of histones gives a specific tag for epigenetic transcription activation. Operates in concert with certain DNA-binding transcriptional activators such as GCN4 or HAP2/3/4. Its acetyltransferase activity seems to be dependent on the association in different multisubunit complexes. Functions as histone acetyltransferase component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. The ADA histone acetyltransferase complex preferentially acetylates nucleosomal histones H3 (to form H3K14ac and H3K18ac) and H2B, leading to transcription regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus.. | |
| Protein Sequence | MVTKHQIEEDHLDGATTDPEVKRVKLENNVEEIQPEQAETNKQEGTDKENKGKFEKETERIGGSEVVTDVEKGIVKFEFDGVEYTFKERPSVVEENEGKIEFRVVNNDNTKENMMVLTGLKNIFQKQLPKMPKEYIARLVYDRSHLSMAVIRKPLTVVGGITYRPFDKREFAEIVFCAISSTEQVRGYGAHLMNHLKDYVRNTSNIKYFLTYADNYAIGYFKKQGFTKEITLDKSIWMGYIKDYEGGTLMQCSMLPRIRYLDAGKILLLQEAALRRKIRTISKSHIVRPGLEQFKDLNNIKPIDPMTIPGLKEAGWTPEMDALAQRPKRGPHDAAIQNILTELQNHAAAWPFLQPVNKEEVPDYYDFIKEPMDLSTMEIKLESNKYQKMEDFIYDARLVFNNCRMYNGENTSYYKYANRLEKFFNNKVKEIPEYSHLID | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 16 | Phosphorylation | EDHLDGATTDPEVKR CCCCCCCCCCHHHHE | 36.94 | 28889911 | |
| 17 | Phosphorylation | DHLDGATTDPEVKRV CCCCCCCCCHHHHEE | 49.36 | 28889911 | |
| 22 | Acetylation | ATTDPEVKRVKLENN CCCCHHHHEEEEECC | 50.22 | 24489116 | |
| 25 | Sumoylation | DPEVKRVKLENNVEE CHHHHEEEEECCCEE | 54.86 | - | |
| 42 | Acetylation | PEQAETNKQEGTDKE HHHHHHHHCCCCCCC | 58.09 | 24489116 | |
| 64 | Phosphorylation | ETERIGGSEVVTDVE HHHEECCEEEEEECC | 23.23 | 28889911 | |
| 130 | Acetylation | IFQKQLPKMPKEYIA HHHHHCCCCCHHHHH | 76.15 | 24489116 | |
| 203 | Phosphorylation | LKDYVRNTSNIKYFL HHHHHHCCCCCCEEE | 16.76 | 19841091 | |
| 204 | Phosphorylation | KDYVRNTSNIKYFLT HHHHHCCCCCCEEEE | 39.86 | 19841091 | |
| 208 | Phosphorylation | RNTSNIKYFLTYADN HCCCCCCEEEEECCH | 10.46 | 19779198 | |
| 211 | Phosphorylation | SNIKYFLTYADNYAI CCCCEEEEECCHHEE | 13.53 | 19841091 | |
| 212 | Phosphorylation | NIKYFLTYADNYAIG CCCEEEEECCHHEEE | 17.69 | 19841091 | |
| 385 | Acetylation | EIKLESNKYQKMEDF EEEECCCCCCCHHHH | 59.34 | 25381059 | |
| 415 | Acetylation | GENTSYYKYANRLEK CCCCHHHHHHHHHHH | 29.65 | 24489116 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
Oops, there are no upstream regulatory protein records of GCN5_YEAST !! | ||||||
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of GCN5_YEAST !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of GCN5_YEAST !! | ||||||
| Kegg Drug | ||||||
|---|---|---|---|---|---|---|
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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